Volume 15 Issue 2
Apr.  2019
Turn off MathJax
Article Contents
Abstract
References
SUN Chengfei, XIE Wenfeng, HU Jie, DONG Junjian, TIAN Yuanyuan, WU Zhaohe, YE Xing. Genetic diversity analysis of three cultured populations of Micropterus salmoides[J]. South China Fisheries Science, 2019, 15(2): 64-71. DOI: 10.12131/20180203
Citation: SUN Chengfei, XIE Wenfeng, HU Jie, DONG Junjian, TIAN Yuanyuan, WU Zhaohe, YE Xing. Genetic diversity analysis of three cultured populations of Micropterus salmoides[J]. South China Fisheries Science, 2019, 15(2): 64-71. DOI: 10.12131/20180203
shu

Genetic diversity analysis of three cultured populations of Micropterus salmoides

  • 1.

    Key Laboratory of Tropical & Subtropical Fisheries Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Pearl River Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

  • 2.

    Shanghai Ocean University, Shanghai 201306, China

  • 3.

    Zhongkai University of Agriculture and Engineering, Guangzhou 510380, China

More Information
  • Received Date: September 10, 2018
  • Revised Date: October 31, 2018
  • Accepted Date: December 16, 2018
  • Available Online: December 19, 2018
    Graphical Abstract
    • Abstract

  • We selected 12 microsatellite primers with specificity and polymorphism from 51 microsatellite primers from GenBank by using an automatic nucleic acid protein analysis system. Fluorescent-labeled primers were synthesized to amplify the three cultured populations of M. salmoides from the main culture area of Guangdong. The results show that among the 12 microsatellite loci, three of them were highly polymorphic, four were moderately polymorphic, and the remaining five showed low polymorphic. The genetic diversity of these three populations was low, and the expected heterozygosity (He) was 0.312 5, 0.360 6 and 0.328 4, respectively. Genetic differentiation index and AMOVA analysis among populations show that the genetic differentiation among populations was low, and 85.83% of genetic variation came from difference among individuals within populations. Genetic distance analysis shows that the genetic distance was small and genetic similarity was rather high among the three cultured populations. Genetic structure analysis shows that the three cultured populations originated from the same subgroup. The results suggest that the genetic diversity of the cultured population of M. salmoides in China has decreased significantly at present. Therefore, great attention should be paid to improving and maintaining genetic diversity of the population during the breeding process.

    • FullText(HTML)
    • References (20)
  • [1]
    BAI J J, LUTZ-CARRILLO D J, QUAN Y C, et al. Taxonomic status and genetic diversity of cultured largemouth bass Micropterus salmoides in China[J]. Aquaculture, 2008, 278(1/2/3/4): 27-30.
    [2]
    农业部渔业局. 中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2018: 25-33.
    [3]
    PRESTI F T, WASKO A P. A review of microsatellite markers and their application on genetic diversity studies in parrots[J]. Open J Genet, 2014, 4(2): 69-77. doi: 10.4236/ojgen.2014.42010
    [4]
    董在杰, 刘念, 傅建军, 等. 6个野生与选育鲤群体的微卫星遗传分析[J]. 南方水产科学, 2018, 14(4): 46-55.
    [5]
    张小谷, 童金苟, 熊邦喜. 微卫星标记在鱼类遗传及育种研究中的应用[J]. 农业生物技术学报, 2006, 14(1): 117-121. doi: 10.3969/j.issn.1674-7968.2006.01.023
    [6]
    梁素娴, 孙效文, 白俊杰, 等. 微卫星标记对中国引进加州鲈养殖群体遗传多样性的分析[J]. 水生生物学报, 2008, 32(5): 694-700.
    [7]
    李镕, 白俊杰, 李胜杰, 等. 大口黑鲈选育群体遗传结构的微卫星分析[J]. 广东海洋大学学报, 2010, 30(3): 11-15. doi: 10.3969/j.issn.1673-9159.2010.03.003
    [8]
    樊佳佳, 白俊杰, 李胜杰, 等. 大口黑鲈微卫星DNA指纹图谱的构建和遗传结构分析[J]. 水生生物学报, 2012, 36(4): 600-609.
    [9]
    黄太寿, 白俊杰, 李胜杰, 等. 我国加州鲈养殖现状和绿色发展问题探讨[J]. 中国水产, 2017(12): 44-47.
    [10]
    NEFF B D, FU P, GROSS M R. Microsatellite evolution in sunfish (Centrarchidae)[J]. Can J Fish Aquat Sci, 1999, 56(7): 1198-1205. doi: 10.1139/f99-068
    [11]
    MALLOY T P, van den BUSSCHE R A, COUGHLIN W D, et al. Isolation and characterization of microsatellite loci in smallmouth bass, Micropterus dolomieu (Teleostei: Centrarchidae), and cross-species amplification in spotted bass, M. punctulatus[J]. Mol Ecol, 2000, 9(11): 1946-1948. doi: 10.1046/j.1365-294x.2000.01096-16.x
    [12]
    RICHARD M, THORPE R S. Highly polymorphic microsatellites in the lacertid Gallotia galloti from the western Canary Islands[J]. Mol Ecol, 2000, 9(11): 1919-1920. doi: 10.1046/j.1365-294x.2000.01052.x
    [13]
    LUTZ-CARRILLO D J, NICE C C, BONNER T H, et al. Admixture analysis of Florida largemouth bass and northern largemouth bass using microsatellite loci[J]. Trans Am Fish Soc, 2006, 135(3): 779-791. doi: 10.1577/T04-221.1
    [14]
    LUTZ-CARRILLO D J, HAGEN C, DUECK L A, et al. Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids[J]. Mol Ecol Resour, 2008, 8(1): 178-184. doi: 10.1111/j.1471-8286.2007.01917.x
    [15]
    TAMURA K, PETERSON D, PETERSON N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Mol Biol Evol, 2011, 28(10): 2731-2739. doi: 10.1093/molbev/msr121
    [16]
    KALINOWSKI S T, TAPER M L, MARSHALL T C. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment[J]. Mol Ecol, 2007, 16(5): 1099-1106. doi: 10.1111/j.1365-294X.2007.03089.x
    [17]
    EXCOFFIER L, LISCHER H E. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows[J]. Mol Ecol Resour, 2010, 10(3): 564-567. doi: 10.1111/men.2010.10.issue-3
    [18]
    EVANNO G, REGNAUT S, GOUDET J. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study[J]. Mol Ecol, 2005, 14(8): 2611-2620. doi: 10.1111/mec.2005.14.issue-8
    [19]
    曾庆凯, 孙成飞, 董浚键, 等. 翘嘴鳜3个不同群体的遗传多样性分析[J]. 基因组学与应用生物学, 2017, 36(8): 3241-3250.
    [20]
    孙成飞, 叶星, 董浚键, 等. 罗氏沼虾6个养殖群体遗传多样性的微卫星分析[J]. 南方水产科学, 2015, 11(2): 20-26. doi: 10.3969/j.issn.2095-0780.2015.02.003
    • Related Articles

  • Cited by

    Periodical cited type(29)

    1. 罗金萍，刘培敏，高权新. 罗氏沼虾良种选育研究进展. 生物资源. 2023(01): 16-27 .
    2. 庄振俊，唐美君，张冬冬，陈文彬，罗明，成永旭，吴旭干，陈晓武. 中华绒螯蟹“长荡湖1号”连续3个世代的遗传多样性分析. 水生生物学报. 2023(09): 1523-1533 .
    3. 邹利，王金龙，李传武，王冬武，曾春芳，刘明求，刘丽，谢敏，曾鸣. 稻田适养品种呆鲤的遗传多样性分析. 水产科学. 2023(05): 795-804 .
    4. 李喜莲，顾志敏，慎佩晶，徐洋，张宇飞，高强，程海华，陈雪峰. 基于RNA-seq技术的罗氏沼虾(Macrobrachium rosenbergii)不同组织转录组比较分析. 海洋与湖沼. 2021(01): 231-241 .
    5. 林明辉，朱华平，苏换换，樊佳佳，池金泉，马冬梅. 3个尖塘鳢引进群体繁育后代的遗传多样性分析. 南方农业学报. 2021(01): 213-220 .
    6. 许珊华，章嘉淇，卢婷，符圆，唐潇，唐琼英，夏正龙，蔡缪荧，高权新，李景芬，杨国梁. 基于微卫星标记的不同种质资源罗氏沼虾遗传多样性研究. 江苏农业科学. 2021(06): 45-51 .
    7. 唐芳，温贝妮，刘红. 不同凡纳滨对虾养殖群体的微卫星遗传多样性分析. 南方农业学报. 2021(04): 1108-1115 .
    8. 苏钰玲，李敏，杨永春，刘佳豪，李振海，阮惠婷，戴嘉格，刘丽，邹柯姝. 南方拟微卫星标记筛选及遗传多样性分析. 水产科学. 2020(02): 224-233 .
    9. 周晓敏，戴习林. 罗氏沼虾SNP标记筛选及不同群体的遗传多样性. 水产学报. 2020(08): 1249-1263 .
    10. 唐琼英，谢巨洪，夏正龙，蔡缪荧，吴云明，白鹿淮，杜厚宽，李景芬，杨国梁. 罗氏沼虾不同育种群体遗传多样性研究. 水生生物学报. 2020(05): 1097-1104 .
    11. 李景芬，夏正龙，栾生，蔡缪荧，罗坤，唐琼英，高权新，孔杰，杨国梁. 五个罗氏沼虾群体遗传多样性的微卫星分析. 水生生物学报. 2020(06): 1208-1214 .
    12. 申淑慧，戴习林. 基于生长和抗逆功能基因SNP分子标记的凡纳滨对虾野生及选育群体遗传多样性分析. 南方农业学报. 2020(11): 2836-2845 .
    13. 王林龙，张秀梅，王展，宋娜，高天翔. 青岛近岸金乌贼繁殖群体形态特征及遗传分化. 中国水产科学. 2019(02): 342-352 .
    14. 孙成飞，谢汶峰，胡婕，董浚键，田园园，吴灶和，叶星. 大口黑鲈3个养殖群体的遗传多样性分析. 南方水产科学. 2019(02): 64-71 . 本站查看
    15. 吕敏，黄光华，李旻，杨琼，卢小花，甘晖，阮志德，黄立斌，杨彦豪，卢天和，马华威. 异型雄性罗氏沼虾遗传多样性的微卫星分析. 水产科学. 2019(03): 355-360 .
    16. 段永楠，刘奕，胡隐昌，刘超，宋红梅，汪学杰，孙金辉，牟希东. 美丽硬仆骨舌鱼全基因组微卫星分布规律特征. 中国农学通报. 2019(23): 152-158 .
    17. 赵彦花，区又君，温久福，李加儿，周慧. 基于微卫星标记的黄唇鱼遗传多样性研究. 南方水产科学. 2019(04): 127-132 . 本站查看
    18. 樊佳佳，白俊杰，李胜杰，马冬梅，姜鹏. 驯食配合饲料的大口黑鲈3个选育世代的遗传多样性分析. 渔业科学进展. 2019(04): 57-64 .
    19. 赵彦花，区又君，温久福，李加儿，周慧. 基于转录组测序技术的黄唇鱼SSR分子标记筛选. 南方农业学报. 2019(09): 2078-2087 .
    20. 宋丹丹，王龙，魏红静，史文竞，朱传坤. 克氏原螯虾微卫星文库构建及多态性分析. 水产科学. 2019(06): 819-826 .
    21. 陈静，宋光同，何吉祥，黄龙，吴本丽，汪翔，武松. 安徽省10个日本沼虾群体遗传多样性微卫星分析. 淡水渔业. 2018(03): 7-12 .
    22. 王传聪，唐修阳，项杰，欧江涛. 罗氏沼虾转录组SSR标记信息分析. 江苏农业科学. 2018(22): 56-59 .
    23. Zhu Bing，Fan Jiajia，Bai Junjie，Jiang Peng. Comparative Analysis of Microsatellite DNA Polymorphism in Gold Grass Carp and Four Grass Carp Populations from China. Animal Husbandry and Feed Science. 2017(06): 379-383+397 .
    24. 袁锐，张朝晖，陈辉，方苹，陈静，刘训猛，吴亚锋，王晶晶. 罗氏沼虾“铁壳”现象及其防控研究进展. 水产科学. 2017(03): 383-390 .
    25. 陈静，何吉祥，黄龙，吴本丽，孙和权，宋光同，陈贵生，王晓健. 3个中华鳖群体遗传多样性的微卫星标记分析. 广东农业科学. 2017(07): 141-146 .
    26. 武小斌，穆淑梅，赵玲玉，康现江，薛建民. 日本沼虾(Macrobrachium nipponense)4个野生群体遗传多样性微卫星分析. 河北大学学报(自然科学版). 2017(02): 161-168 .
    27. 朱冰，樊佳佳，白俊杰，姜鹏. 金草鱼与中国4个草鱼群体的微卫星多态性比较分析. 南方水产科学. 2017(02): 51-58 . 本站查看
    28. 刘磊，彭士明，高权新，张晨捷，施兆鸿. 基于银鲳RNA-seq数据中SSR标记的信息分析. 安徽农业科学. 2016(28): 102-105 .
    29. 钟丹丹，林勇，宾石玉，余艳玲，陈忠，曾兰，杜雪松，张永德. 两个罗氏沼虾种群的遗传多样性研究. 广东农业科学. 2015(24): 140-145 .

    Other cited types(17)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(46)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return