Volume 15 Issue 2
Apr.  2019
Turn off MathJax
Article Contents
Abstract
References
Related Articles
SUN Chengfei, XIE Wenfeng, HU Jie, DONG Junjian, TIAN Yuanyuan, WU Zhaohe, YE Xing. Genetic diversity analysis of three cultured populations of Micropterus salmoides[J]. South China Fisheries Science, 2019, 15(2): 64-71. DOI: 10.12131/20180203
Citation: SUN Chengfei, XIE Wenfeng, HU Jie, DONG Junjian, TIAN Yuanyuan, WU Zhaohe, YE Xing. Genetic diversity analysis of three cultured populations of Micropterus salmoides[J]. South China Fisheries Science, 2019, 15(2): 64-71. DOI: 10.12131/20180203
shu

Genetic diversity analysis of three cultured populations of Micropterus salmoides

  • 1.

    Key Laboratory of Tropical & Subtropical Fisheries Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Pearl River Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

  • 2.

    Shanghai Ocean University, Shanghai 201306, China

  • 3.

    Zhongkai University of Agriculture and Engineering, Guangzhou 510380, China

More Information
  • Received Date: September 10, 2018
  • Revised Date: October 31, 2018
  • Accepted Date: December 16, 2018
  • Available Online: December 19, 2018
    Graphical Abstract
    • Abstract

  • We selected 12 microsatellite primers with specificity and polymorphism from 51 microsatellite primers from GenBank by using an automatic nucleic acid protein analysis system. Fluorescent-labeled primers were synthesized to amplify the three cultured populations of M. salmoides from the main culture area of Guangdong. The results show that among the 12 microsatellite loci, three of them were highly polymorphic, four were moderately polymorphic, and the remaining five showed low polymorphic. The genetic diversity of these three populations was low, and the expected heterozygosity (He) was 0.312 5, 0.360 6 and 0.328 4, respectively. Genetic differentiation index and AMOVA analysis among populations show that the genetic differentiation among populations was low, and 85.83% of genetic variation came from difference among individuals within populations. Genetic distance analysis shows that the genetic distance was small and genetic similarity was rather high among the three cultured populations. Genetic structure analysis shows that the three cultured populations originated from the same subgroup. The results suggest that the genetic diversity of the cultured population of M. salmoides in China has decreased significantly at present. Therefore, great attention should be paid to improving and maintaining genetic diversity of the population during the breeding process.

    • FullText(HTML)
    • References (20)
  • [1]
    BAI J J, LUTZ-CARRILLO D J, QUAN Y C, et al. Taxonomic status and genetic diversity of cultured largemouth bass Micropterus salmoides in China[J]. Aquaculture, 2008, 278(1/2/3/4): 27-30.
    [2]
    农业部渔业局. 中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2018: 25-33.
    [3]
    PRESTI F T, WASKO A P. A review of microsatellite markers and their application on genetic diversity studies in parrots[J]. Open J Genet, 2014, 4(2): 69-77. doi: 10.4236/ojgen.2014.42010
    [4]
    董在杰, 刘念, 傅建军, 等. 6个野生与选育鲤群体的微卫星遗传分析[J]. 南方水产科学, 2018, 14(4): 46-55.
    [5]
    张小谷, 童金苟, 熊邦喜. 微卫星标记在鱼类遗传及育种研究中的应用[J]. 农业生物技术学报, 2006, 14(1): 117-121. doi: 10.3969/j.issn.1674-7968.2006.01.023
    [6]
    梁素娴, 孙效文, 白俊杰, 等. 微卫星标记对中国引进加州鲈养殖群体遗传多样性的分析[J]. 水生生物学报, 2008, 32(5): 694-700.
    [7]
    李镕, 白俊杰, 李胜杰, 等. 大口黑鲈选育群体遗传结构的微卫星分析[J]. 广东海洋大学学报, 2010, 30(3): 11-15. doi: 10.3969/j.issn.1673-9159.2010.03.003
    [8]
    樊佳佳, 白俊杰, 李胜杰, 等. 大口黑鲈微卫星DNA指纹图谱的构建和遗传结构分析[J]. 水生生物学报, 2012, 36(4): 600-609.
    [9]
    黄太寿, 白俊杰, 李胜杰, 等. 我国加州鲈养殖现状和绿色发展问题探讨[J]. 中国水产, 2017(12): 44-47.
    [10]
    NEFF B D, FU P, GROSS M R. Microsatellite evolution in sunfish (Centrarchidae)[J]. Can J Fish Aquat Sci, 1999, 56(7): 1198-1205. doi: 10.1139/f99-068
    [11]
    MALLOY T P, van den BUSSCHE R A, COUGHLIN W D, et al. Isolation and characterization of microsatellite loci in smallmouth bass, Micropterus dolomieu (Teleostei: Centrarchidae), and cross-species amplification in spotted bass, M. punctulatus[J]. Mol Ecol, 2000, 9(11): 1946-1948. doi: 10.1046/j.1365-294x.2000.01096-16.x
    [12]
    RICHARD M, THORPE R S. Highly polymorphic microsatellites in the lacertid Gallotia galloti from the western Canary Islands[J]. Mol Ecol, 2000, 9(11): 1919-1920. doi: 10.1046/j.1365-294x.2000.01052.x
    [13]
    LUTZ-CARRILLO D J, NICE C C, BONNER T H, et al. Admixture analysis of Florida largemouth bass and northern largemouth bass using microsatellite loci[J]. Trans Am Fish Soc, 2006, 135(3): 779-791. doi: 10.1577/T04-221.1
    [14]
    LUTZ-CARRILLO D J, HAGEN C, DUECK L A, et al. Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids[J]. Mol Ecol Resour, 2008, 8(1): 178-184. doi: 10.1111/j.1471-8286.2007.01917.x
    [15]
    TAMURA K, PETERSON D, PETERSON N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Mol Biol Evol, 2011, 28(10): 2731-2739. doi: 10.1093/molbev/msr121
    [16]
    KALINOWSKI S T, TAPER M L, MARSHALL T C. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment[J]. Mol Ecol, 2007, 16(5): 1099-1106. doi: 10.1111/j.1365-294X.2007.03089.x
    [17]
    EXCOFFIER L, LISCHER H E. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows[J]. Mol Ecol Resour, 2010, 10(3): 564-567. doi: 10.1111/men.2010.10.issue-3
    [18]
    EVANNO G, REGNAUT S, GOUDET J. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study[J]. Mol Ecol, 2005, 14(8): 2611-2620. doi: 10.1111/mec.2005.14.issue-8
    [19]
    曾庆凯, 孙成飞, 董浚键, 等. 翘嘴鳜3个不同群体的遗传多样性分析[J]. 基因组学与应用生物学, 2017, 36(8): 3241-3250.
    [20]
    孙成飞, 叶星, 董浚键, 等. 罗氏沼虾6个养殖群体遗传多样性的微卫星分析[J]. 南方水产科学, 2015, 11(2): 20-26. doi: 10.3969/j.issn.2095-0780.2015.02.003
    • Related Articles

  • Related Articles

    [1]MAI Guangming, CHEN Zhijie, WANG Xuefeng, XIAO Yayuan, LI Chunhou. Spatial pattern of fish taxonomic diversity along coastal waters in northern South China Sea[J]. South China Fisheries Science, 2022, 18(3): 38-47. DOI: 10.12131/20210117
    [2]PENG Lu, JIANG Yan’e, XU Shannan, CHEN Zuozhi. Otolith morphology of Nemipterus virgatus and its relation to body length and mass in continental shelf of northern South China Sea[J]. South China Fisheries Science, 2018, 14(6): 27-33. DOI: 10.12131/20180049
    [3]FAN Jiangtao, FENG Xue, CHEN Zuozhi. Fishing ground analysis of Japanese horse mackerel in northern South China Sea based on vertical structure of water temperature[J]. South China Fisheries Science, 2018, 14(2): 124-128. DOI: 10.3969/j.issn.2095-0780.2018.02.017
    [4]LIU Huaxue, ZHANG Wenbo, XU Jun, LI Chunhou, HUANG Honghui. Stable carbon isotope of size fractionated zooplankton in northern South China Sea[J]. South China Fisheries Science, 2018, 14(2): 36-40. DOI: 10.3969/j.issn.2095-0780.2018.02.005
    [5]CAI Yancong, XU Shannan, CHEN Zuozhi, XU Youwei, JIANG Yan'e, YANG Changping. Current status of community structure and diversity of fishery resources in offshore northern South China Sea[J]. South China Fisheries Science, 2018, 14(2): 10-18. DOI: 10.3969/j.issn.2095-0780.2018.02.002
    [6]WANG Yan, CHEN Zuozhi, ZHANG Jun, ZHANG Guosheng, TANG Yong, XU Shannan, JIANG Yan′e. Morphologic features of otolith of Myctophum asperum in continental shelf slope of northern South China Sea[J]. South China Fisheries Science, 2017, 13(6): 65-72. DOI: 10.3969/j.issn.2095-0780.2017.06.008
    [7]ZHANG Wenbo, LIU Huaxue, GONG Xiuyu, SHI Rongjun, HUANG Honghui. Stable nitrogen isotope of size fractionated zooplankton in northern South China Sea[J]. South China Fisheries Science, 2017, 13(6): 48-55. DOI: 10.3969/j.issn.2095-0780.2017.06.006
    [8]ZHAO Minghui, HUANG Honghui, QI Zhanhui, LI Chunhou, LIN Zhaojin, CHEN Guizhu. Landscape pattern analysis of plankton in northern South China Sea[J]. South China Fisheries Science, 2010, 6(6): 41-45. DOI: 10.3969/j.issn.1673-2227.2010.06.007
    [9]YANG Lin, ZHANG Xufeng, TAN Yongguang, ZHANG Peng. Analysis on the catch composition of light-purse seine in the northern South China Sea[J]. South China Fisheries Science, 2009, 5(6): 65-70. DOI: 10.3969/j.issn.1673-2227.2009.06.012
    [10]JIANG Yane, LIN Zhaojin, HUANG Zirong. Biodiversity of fishery resources in the continental shelf of northern South China Sea[J]. South China Fisheries Science, 2009, 5(5): 32-37. DOI: 10.3969/j.issn.1673-2227.2009.05.006

Catalog

    Get Citation
    PDF
    XML
    Article views (5029) PDF downloads (67) Cited by()
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return