Volume 15 Issue 2
Apr.  2019
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SUN Chengfei, XIE Wenfeng, HU Jie, DONG Junjian, TIAN Yuanyuan, WU Zhaohe, YE Xing. Genetic diversity analysis of three cultured populations of Micropterus salmoides[J]. South China Fisheries Science, 2019, 15(2): 64-71. DOI: 10.12131/20180203
Citation: SUN Chengfei, XIE Wenfeng, HU Jie, DONG Junjian, TIAN Yuanyuan, WU Zhaohe, YE Xing. Genetic diversity analysis of three cultured populations of Micropterus salmoides[J]. South China Fisheries Science, 2019, 15(2): 64-71. DOI: 10.12131/20180203
shu

Genetic diversity analysis of three cultured populations of Micropterus salmoides

  • 1.

    Key Laboratory of Tropical & Subtropical Fisheries Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Pearl River Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

  • 2.

    Shanghai Ocean University, Shanghai 201306, China

  • 3.

    Zhongkai University of Agriculture and Engineering, Guangzhou 510380, China

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  • Received Date: September 10, 2018
  • Revised Date: October 31, 2018
  • Accepted Date: December 16, 2018
  • Available Online: December 19, 2018
    Graphical Abstract
    • Abstract

  • We selected 12 microsatellite primers with specificity and polymorphism from 51 microsatellite primers from GenBank by using an automatic nucleic acid protein analysis system. Fluorescent-labeled primers were synthesized to amplify the three cultured populations of M. salmoides from the main culture area of Guangdong. The results show that among the 12 microsatellite loci, three of them were highly polymorphic, four were moderately polymorphic, and the remaining five showed low polymorphic. The genetic diversity of these three populations was low, and the expected heterozygosity (He) was 0.312 5, 0.360 6 and 0.328 4, respectively. Genetic differentiation index and AMOVA analysis among populations show that the genetic differentiation among populations was low, and 85.83% of genetic variation came from difference among individuals within populations. Genetic distance analysis shows that the genetic distance was small and genetic similarity was rather high among the three cultured populations. Genetic structure analysis shows that the three cultured populations originated from the same subgroup. The results suggest that the genetic diversity of the cultured population of M. salmoides in China has decreased significantly at present. Therefore, great attention should be paid to improving and maintaining genetic diversity of the population during the breeding process.

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