Volume 18 Issue 6
Dec.  2022
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GAO Jie, GUO Huayang, LIU Mingjian, LIU Baosuo, ZHU Kecheng, ZHANG Nan, GUO Liang, ZHANG Dianchang. Response of caspase genes to Streptococcus agalactiae in cells of spleen of golden pompano (Trachinotus ovatus)[J]. South China Fisheries Science, 2022, 18(6): 44-51. DOI: 10.12131/20220099
Citation: GAO Jie, GUO Huayang, LIU Mingjian, LIU Baosuo, ZHU Kecheng, ZHANG Nan, GUO Liang, ZHANG Dianchang. Response of caspase genes to Streptococcus agalactiae in cells of spleen of golden pompano (Trachinotus ovatus)[J]. South China Fisheries Science, 2022, 18(6): 44-51. DOI: 10.12131/20220099
shu

Response of caspase genes to Streptococcus agalactiae in cells of spleen of golden pompano (Trachinotus ovatus)

  • 1.

    College of Oceanography, Hebei Agricultural University, Qinhuangdao 066003, China

  • 2.

    Sanya Tropical Fisheries Research Institute, Sanya 572019, China

  • 3.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 4.

    Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China

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  • Received Date: April 10, 2022
  • Revised Date: May 04, 2022
  • Accepted Date: June 12, 2022
  • Available Online: June 17, 2022
    Graphical Abstract
    • Abstract
  • In order to explore the response of apoptosis related genes in the spleen of Trachinotus ovatus infected by Streptococcus agalactiae, we infected the spleen tissues of T. ovatus by S. agalactiae for a pathological observation. The blood routine indexes of the fish before and after infection, as well as different times (0 h, 6 h,12 h, 24 h, 48 h, 72 h, 96 h, 120 h) after infection, the serum lactate dehydrogenase (LDH), spleen alkaline phosphatase (ALP), and acid phosphatase (ACP) and quantitative analysis of Caspase-3, Caspase-8, and Caspase-9 genes. The results reveal that after artificial infection with S. agalactiae, the body of T. ovatus showed obvious pathological changes, mainly manifested as protruding eyes, turbidity and bleeding. Histopathological observation shows that the spleen tissue had inflammatory lesions. Compared with the control group, the number of red blood cells (RBC), hemoglobin concentration (Hgb) and hematocrit (HCT) in the infected group decreased significantly, while the number of white blood cells (WBC), neutrophils (NE) and lymphocytes (LYM) increased, and the mean red blood cell volume (MCV) increased. The activities of serum LDH, spleen ALP and ACP were up-regulated with time. The expressions of Caspase-3, Caspase-8 and Caspase-9 were significantly up-regulated in spleen. The results show that the body of T. ovatus infected by S. agalactiae had an obvious immune response.
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