Volume 16 Issue 5
Oct.  2020
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HU Yuting, JIANG He, DUAN Guoqing, ZHOU Huaxing, LING Jun, WANG Huan. Genetic diversity analysis of Pelteobagrus fulvidraco from two major drainage systems in Anhui Province based on microsatellite markers[J]. South China Fisheries Science, 2020, 16(5): 33-41. DOI: 10.12131/20200063
Citation: HU Yuting, JIANG He, DUAN Guoqing, ZHOU Huaxing, LING Jun, WANG Huan. Genetic diversity analysis of Pelteobagrus fulvidraco from two major drainage systems in Anhui Province based on microsatellite markers[J]. South China Fisheries Science, 2020, 16(5): 33-41. DOI: 10.12131/20200063
shu

Genetic diversity analysis of Pelteobagrus fulvidraco from two major drainage systems in Anhui Province based on microsatellite markers

  • Fishery Institute of Anhui Academy of Agricultural Sciences/Anhui Province Key Laboratory of Aquaculture & Stock Enhancement, Hefei 230031, China

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  • Received Date: April 02, 2020
  • Revised Date: May 10, 2020
  • Accepted Date: May 29, 2020
  • Available Online: September 27, 2020
    Graphical Abstract
    • Abstract

  • We collected 254 individuals of nine wild Pelteobagrus fulvidraco populations from Yangtze River and Huaihe River in Anhui Province, then genotyped them by using ten microsatellite markers. The result shows a high level of genetic diversity. Altogether 245 alleles had been detected in ten loci with an average of effective allele number (Ne) of 9.75 per locus. In all P. fulvidraco populations, the allele number (Na) was 5.20−14.80 and the Ne was 2.64−8.93; the observed heterozygosity (Ho) was 0.496−0.671 and the expected heterozygosity (He) was 0.557−0.818; the polymorphism information content (PIC) was 0.500−0.790. The molecular variance analysis (AMOVA) exhibited only 8.15% genetic variation among the populations and showed a low and middle level genetic differentiation (FST: 0.006−0.236). The UPGMA tree of P. fulvidraco based on Nei's genetic distance (0.079−0.640) and population genetic structure analysis based on Structure software both indicate that the nine populations belonged to four or five genetic lineages; ST, FN and MC populations were all independent groups which were genetically distantly related to the others. The results reveal that there is genetic differentiation among the nine P. fulvidraco populations and some populations have experienced bottleneck effects, but the wild resources still have high genetic diversity.

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