Volume 14 Issue 4
Jul.  2018
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WU Yanyan, ZHAO Zhixia, LI Laihao, HAO Shuxian, DENG Jianchao, HU Xiao. Effect of adding exogenous enzymes on quality of pickled tilapia[J]. South China Fisheries Science, 2018, 14(4): 102-111. DOI: 10.3969/j.issn.2095-0780.2018.04.013
Citation: WU Yanyan, ZHAO Zhixia, LI Laihao, HAO Shuxian, DENG Jianchao, HU Xiao. Effect of adding exogenous enzymes on quality of pickled tilapia[J]. South China Fisheries Science, 2018, 14(4): 102-111. DOI: 10.3969/j.issn.2095-0780.2018.04.013
shu

Effect of adding exogenous enzymes on quality of pickled tilapia

  • 1.

    Key Laboratory of Aquatic Product Processing, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

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  • Received Date: February 10, 2018
  • Revised Date: April 03, 2018
  • Available Online: December 04, 2018
    Graphical Abstract
    • Abstract
  • To explore the effects of adding flavor protease, neutral protease and lipase on the quality of pickled tilapia (Oreochromis mossambicus), we studied their amino acid nitrogen content, fatty acid composition and sensory analysis by comparing the volatile flavor substances in fresh tilapia fillets, pickled tilapia fillets, low salt and adding exogenous enzymes tilapia fillets. The results show that the optimal concentration of flavor protease was 0.010 0%, and the amino nitrogen concentration was 0.75 mg·g–1, 19.05% higher than that of the control, and the relative contents of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) were 35.65%, 23.78% and 40.57%, respectively, and the sensory score was 89, 10.56% higher than that of the control. The optimal concentration of neutral protease was 0.010 0%, and the amino nitrogen concentration was 0.81 mg·g–1, 28.57% higher than that of the control, and the relative contents of SFA, MUFA and PUFA were 34.24%, 24.82% and 40.94%, respectively, and the sensory score was 88.5, 10.63% higher than that of the control. The optimal concentration of lipase was 0.010 0%, and the amino nitrogen concentration was 0.67 mg·g–1, 4.69% higher than that of the control, and the relative contents of SFA, MUFA and PUFA were 33.97%, 22.20% and 43.85%, respectively, and the sensory score was 88.5, 11.32% higher than that of the control. It is proved that the mix salted of the three optimal concentrations of exogenous enzymes can increase the formation of flavor substances, especially alcohols and ketones, increase the efficiency of curing effectively, and improve the flavor of salted fish.
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    • References (31)
  • [1]
    赵志霞, 吴燕燕, 李来好, 等. 我国罗非鱼加工研究现状[J]. 食品工业科技, 2017, 38(9): 363-367, 373.
    [2]
    KEMP C M, SENSKY P L, BARDSLEY R G, et al. Tenderness: an enzymatic view[J]. Meat Sci, 2010, 84(2): 248-256.
    [3]
    封莉, 邓绍林, 黄明, 等. 脂肪酶对中式香肠脂肪降解、氧化和风味的影响[J]. 食品科学, 2015, 36(1): 51-58.
    [4]
    朱建军, 王晓宇, 胡萍, 等. 外源蛋白酶对黔式腊肉风味的影响[J]. 肉类研究, 2013, 27(8): 16-20.
    [5]
    ANSORENA D, ASTIASARÁN I, BELLO J. Influence of the simultaneous addition of the protease flavourzyme and the lipase novozym 677BG on dry fermented sausage compounds extracted by SDE and analyzed by GC-MS[J]. J Agric Food Chem, 2000, 48(6): 2395-2400.
    [6]
    FENG L, QIAO Y, ZOU Y, et al. Effect of flavourzyme on proteolysis, antioxidant capacity and sensory attributes of Chinese sausage[J]. Meat Sci, 2014, 98(1): 34-40.
    [7]
    BRONCANO J M, TIMON M L, PARRA V, et al. Use of proteases to improve oxidative stability of fermented sausages by increasing low molecular weight compounds with antioxidant activity[J]. Food Res Int, 2011, 44(9): 2655-2659.
    [8]
    赵志霞, 吴燕燕, 李来好, 等. 低盐罗非鱼片快速腌制的工艺研究[J]. 南方水产科学, 2017, 13(6): 105-114.
    [9]
    杨贤庆, 杨丽芝, 黄卉, 等. 南海鸢乌贼墨汁营养成分分析与评价[J]. 南方水产科学, 2015, 11(5): 138-142.
    [10]
    NEFF M R, BHAVSAR S P, BRAEKEVELT E, et al. Effects of different cooking methods on fatty acid profiles in four freshwater fishes from the Laurentian Great Lakes region[J]. Food Chem, 2014, 164(20): 544-550.
    [11]
    TIMÓN M L, VENTANAS J, CARRAPISO A I, et al. Subcutaneous and intermuscular fat characterisation of dry-cured Iberian hams[J]. Meat Sci, 2001, 58(1): 85-91.
    [12]
    COUTRON G C, GANDEMER G, ROUSSET S, et al. Reducing salt content of dry-cured ham: effect on lipid composition and sensory attributes[J]. Food Chem, 1999, 64(1): 13-19.
    [13]
    ZHANG S, ZHANG C, QIAO Y, et al. Effect of flavourzyme on proteolysis, antioxidant activity and sensory qualities of Cantonese bacon[J]. Food Chem, 2017, 237: 779-785.
    [14]
    曾少葵, 郑琪, 杨思新, 等. 混合乳酸菌发酵改善罗非鱼蛋白酶解液风味的研究[J]. 南方水产, 2010, 6(3): 24-31.
    [15]
    穆建稳, 董全, 李洪军. 腊牛肉加工过程中中性蛋白酶对蛋白质变化影响的研究[J]. 肉类工业, 2011(7): 19-21.
    [16]
    李静, 刘嘉俊, 邓毛程. 低值虾制备呈味肽的酶解工艺[J]. 中国调味品, 2016, 41(1): 112-116.
    [17]
    JIN G F, ZHANG J H, YU X, et al. Lipolysis and lipid oxidation in bacon during curing and drying-ripening[J]. Food Chem, 2010, 123(2): 465-471.
    [18]
    吴燕燕, 曹松敏, 魏涯, 等. 腌制鱼类中内源性酶类对制品品质影响的研究进展[J]. 食品工业科技, 2016, 37(8): 358-363.
    [19]
    吴若娜, 李诚, 付刚, 等. 外源脂肪酶对腊鸭腿品质的影响[J]. 食品工业科技, 2013, 34(19): 161-164, 168.
    [20]
    SELLI S, CAYHAN G G. Analysis of volatile compounds of wild gilthead sea bream (Sparus aurata) by simultaneous distillation-extraction (SDE) and GC-MS[J]. Microchem J, 2009, 93(2): 232-235.
    [21]
    王璐, 王锡昌, 刘源. 草鱼不同部位气味研究[J]. 食品科学, 2010, 31(6): 158-164.
    [22]
    王三丽. 外源酶调控生产火腿风味调味料的研究[D]. 无锡: 江南大学, 2008: 50-55.
    [23]
    蔡瑞康, 吴佳佳, 朱建龙, 等. 大黄鱼糟制过程中风味物质及风味活性物质分析[J]. 中国食品学报, 2017, 17(2): 264-273.
    [24]
    金燕, 杨荣华, 周凌霄, 等. 蟹肉挥发性成分的研究[J]. 中国食品学报, 2011, 11(1): 233-238.
    [25]
    吴燕燕, 王悦齐, 李来好, 等. 基于电子鼻与HS-SPME-GC-MS技术分析不同处理方式腌干带鱼挥发性风味成分[J]. 水产学报, 2016, 40(12): 1931-1940.
    [26]
    FRATINI G, LOIS S, PAZOS M, et al. Volatile profile of Atlantic shellfish species by HS-SPME GC/MS[J]. Food Res Int, 2012, 48(2): 856-865.
    [27]
    潘冰燕, 鲁晓翔, 张鹏, 等. GC-MS结合电子鼻分析1-MCP处理对线椒低温贮藏期挥发性物质的影响[J]. 食品科学, 2016, 37(2): 238-243.
    [28]
    ANDRÉS A I, CAVA R, RUIZ J. Monitoring volatile compounds during dry-cured ham ripening by solid-phase microextraction coupled to a new direct-extraction device[J]. J Chromatogr A, 2002, 963(1/2): 83-88.
    [29]
    FRANK D, BALL A, HUGHES J, et al. Sensory and flavor chemistry characteristics of australian beef: influence of intramuscular fat, feed, and breed[J]. J Agric Food Chem, 2016, 64(21): 4299-4311.
    [30]
    INAGAKI S A Y. Characterization of volatile components causing the characteristic flavor of wagyu beef (Japanese black cattle)[J]. J Agric Food Chem, 2017, 65(39): 8691-8695.
    [31]
    ZHAO Q L, SHEN Q, GUO R, et al. Characterization of flavor properties from fish (Collichthys niveatus) through enzymatic hydrolysis and the maillard reaction[J]. J Aquat Food Prod Technol, 2016, 25(4): 482-495.
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