The influence of temperature, light and nutrient solution on the mature and spore release for Gloiopeltis furcata and G.tenax was investigated in the laboratory. G.furcata could mature and release spores at 10~20℃ while G.tenax could mature and release spores at 12~24℃. This two species of Gloiopeltis could mature and release spores at illuminance 2 500~8 500 lx. For no nutrient addition to the stocking water the two species of Gloiopeltis could mature and release spores, while nutrient addition too high (80 mg·L^-1 NaNO₃ and 8 mg·L^-1 KH₂PO₄) it was not good for the mature and spores release of this two species alga.

A series of culture experiments of the subtidal brown alga Sargassum horneri was performed during 2005~2007, including development of receptacles, discharge of eggs, impact of environmental factors on the development of the zygotes. Artificial seedlings derived from zygotes were obtained from selected individuals sampled from wild populations at Nanji Island, Zhejiang Province, China. Different substrates were tested for settlement of zygotes. Field in situ observation of the growth and reproduction of selected individuals was performed. Based on these results, we concluded that field population of this alga sustains itself principally through sexual reproduction. These results lay the basis of artificial seedling reproduction of this alga.

Changes in fish year-class strength have been attributed to year-to-year variability in environmental conditions, such as sea surface temperature (SST) and phosphate concentration, and spawning stock biomass(SSB).In this study, we examined the relationship between environmental factors, SSB and recruitment for the anchovy (Engraulis japonicas) stock in the Yellow Sea using a traditional stock-recruitment Ricker model. The recruitment fluctuation of the anchovy stock in the Yellow Sea was successfully reproduced by the Ricker stock-recruitment model with environmental factors. The findings suggest that the impacts of SST and phosphate concentration observed at the Qianliyan Marine Environment Monitoring Station in the Yellow Sea on the anchovy stock in the Yellow Sea are very important.

Based on the scientific observer′s surveys in tropical Eastern Pacific Ocean covering areas 03°S~17°S, 96°W~146°W and 05°N~10°S, 134°W~173°W during July~Nov. 2003 and Feb. ~Nov. 2006 respectively, 202 specimens of crocodile sharks (Pseudocarcharias kamoharai) were collected and analysed. The result showed that the relationship between total length and total weight of crocodile is W=9×10^-5TL^2.3116 for female and W=9×10^-6TL^2.9007 for male. The sex ratio of crocodile shark was nearly 1:1. Female crocodile shark′s total gonad weight was increased with the growth of total length, female crocodile shark adult matured at about 89 cm, males adult about 85~100 cm. The brood of crocodile is 3.85, and size at birth is 40 cm.

Based on otter trawl catch rates of five surveys conducted by South China Sea Fisheries Research Institute from 1961 to 1999, changes in demersal trawling fishery resources in the northern South China Sea were analyzed. The results indicated that, in over 30 years from early 1960s to late 1990s, the trawl catch rates have declined noticeably by 72% and 81% separately in the northern shelf and Beibu Gulf of the South China Sea. The catch rates have dropped by 71%, 74% and 70% respectively in the inshore, offshore, and outer shelf of the northern shelf. The demersal fishery resources in all areas of the northern South China Sea have been over-exploited, and more severe overfishing occurred in the inshore and offshore waters than in the outer shelf.

Because of their simple form and relatively undemanding of data, surplus production models were widely used in fishery stock assessment. This paper used a continuous Fox-form production model to analyze 3 simulated fisheries and the North Atlantic swordfish fishery. The results of the simulated fisheries were compared to determine what fishery data condition is most suitable for the continuous Fox-form production model. Monte Carlo simulation analysis showed that the model performance for fishery 3 was better than those for the other two simulated fisheries. This may be because the exploitation process of fishery 3 is more complete and fishery 3 data contains more information. When white noise was less than 10%, the percentage biases were small, less than 3.3%. When the white noise was 50%, the percentage biases were large with the largest of 36.7%.

During the exploitation of purpleback flying squid Sthenoteuthis oualaniensis resources in the northwestern Indian Ocean (13°N~20°N, 59°E~64°E) from September to December in 2004 and from March to May in 2005, 48 individuals muscle samples collected from 12 stations were used for population genetic structure identification by Random Amplified Polymorphic DNA (RAPD) analysis. The results showed that the purpleback flying squid in the northwestern Indian Ocean could be divided into two populations by UPGMA clustering analysis.One population distributed in the area of 13°N~18°N, while another population distributed in the area of 18°N~20°N. The results showed that high genetic variations were found within population and the coefficient of genetic differentiation between the two populations was 0.2150, which indicated that quite different genetic background existed in the two populations. Shannon′s diversity index was 0.3676±0.1801. Genetic distance between the two populations was 0.1338, and genetic similarity was 0.8748.

Expression patterns of IGF-ⅠmRNA in different tissues of mud carp (Cirrhinus molitorella)were studied using semi-quantitative RT-PCR method and the effects of recombinant mud carp growth hormone(rmcGH)on expression of IGF-Ⅰin mud carp were investigated. The IGF-ⅠmRNA was detected successfully in liver, kidney, brain, intestine, gill, spleen, gonad, and heart, and no IGF-ⅠmRNA was detected in skin and muscle. The IGF-Ⅰ mRNA detected was higher in liver than in other tissues. Mud carp were received either intra-peritoneal injections of rmcGH (120 μg·g^-1 body weight) or vehicle, tissue samples and blood were collected 12 hour later. Total RNA was isolated and assayed for IGF-ⅠmRNA using semi-quantitative RT-PCR. Blood was extracted to determine the levels of IGF-Ⅰ in serum. IGF-Ⅰlevels increased from 145.59±21.84 to 247.71±2.83 ng·mL^-1 (P=0.043) in the serum after rmcGH injection.

Genetic diversity and molecular evolution of five species of grouper, viz.Epinephelus lanceolatus, Cromileptes altivelis, E.fuscoguttatus, E.coioides and E.fario, were detected using RAPD technique. A total 120 random primers were evaluated for the usefulness. And thereinto, 19 random primers were selected according to the effect of PCR of genomic DNA of 5 species of grouper. The results showed as the followings: the polymorphic loci ratio in E.lanceolatus, C.altivelis, E.fuscoguttatus, E.coioides and E.fario (P) were 64.23%, 72.61%, 60.34%, 69.97% and 73.94%, respectively. The mean genetic similarity index of intra-species (S) were 0.8238, 0.8110, 0.8345, 0.8277, 0.8064, respectively, the mean genetic distances of intra-species (D) were 0.1762, 0.1890, 0.1655, 0.1723 and 0.1936, respectively, Nei′s gene diversity (H) were 0.1189, 0.1364, 0.1028, 0.1439 and 0.1648, respectively, and Shannon′s information index (H_i) were 0.1801, 0.1992, 0.1530, 0.2100, 0.2434, respectively. The mean genetic distances of inter-species (D_xy) were between 0.3964~0.6085.

Nutritional composition in visceral mass of wild Hemifusus tuba was determined and evaluated. Each of the snails was high-protein and low-fat, and the pattern of essential amino acids was approximately similar to the ideal pattern of FAO/WHO. The content of protein in the viscera was 80.777%, containing 17 kinds of amino acids including 4 kinds of flavour amino acids. The content of amino acids accounted 51.42% of the dry weight. The total point of methionine and cysteine, the essential amino acids, was up to 85.94. There was plenty of flavour amino acids, which accounted 48.62% of the gross amnio acids, and the EAA/TAA was 40.57%, the EAA/NTAA was 68.72%. All kinds of fatty acids could be detected from the crude fats, and there has high level of unsaturated fatty acid in the total fatty acid. The results indicated that H.tuba is characterized by high nutrition and sanitarian compound.

The extraction of natural astaxanthin from shrimp wastes was studied. The extraction technology of astaxanthin with the vacuum cavitation was discussed in this article. The influences of extraction solvent, concentration of solvent, time and ventilation on extracting ratios were studied, and the best extraction parameters were obtained by orthogonal test. The extraction solvent was 80% ethanol, the extraction time was 35 minutes and the ventilation was 0.2 m³·h^-1. The effect and efficiency of the vacuum cavitations were compared with other cell-broken extraction in common use. It showed that the vacuum cavitations extraction is superior to others and it is a new extraction method fitting for industry.

As an important topic of marine environment research, seawater exchange research is helpful to assessment of marine environmental quality and the research of marine ecological changing mechanism of artificial reefs area. In this paper, the advances in the research on seawater exchange ability were summarized. The water exchange models and methods were analyzed and compared, and the research hot spot and tendency of water exchange in the field of embayment and artificial reefs in the future were discussed.