Abstract: In order to achieve all information about enzyme in Tilapia organ, it's necessary to make a comprehensive study on enzyme. Enzyme activity and enzyme quantity were used as parameters to evaluate enzyme score. Protease, chymosin, urease, superoxide dismutase (SOD), cholinesterase, acetylcholinesterase, alkaline phosphatase and acidic phosphatase in Tilapia organs were studied in this paper. The results showed that the enzyme activity and enzyme quantity of protease, chymosin, SOD and alkaline phosphatase were higher than others, and it's worthy to be studied further.