Abstract: To determine the appropriate anesthetic method and dose for juvenile Micropterus salmoides in laboratory, we compared the hydrostatic anesthesia effects of two common fish anesthetics (MS-222 and eugenol) on the juveniles with different concentrations (40 mg·L^–1, 50 mg·L^–1, 60 mg·L^–1, 70 mg·L^–1, 80 mg·L^–1, 90 mg·L^–1, 100 mg·L^–1 and 8 mg·L^–1, 10 mg·L^–1, 12 mg·L^–1, 14 mg·L^–1, 16 mg·L^–1, 18 mg·L^–1, 20 mg·L^–1). The results show: 1. With increasing concentrations of the two anesthetics, the anesthetic time shortened but the recovery time prolonged. 2. The optimal anesthetic concentrations of MS-222 and eugenol were 70 mg·L^–1 and 16 mg·L^–1, respectively, while the optimal anesthetic transportation concentrations of the two anesthetics were 50 mg·L^–1 and 10 mg·L^–1, respectively. Then we carried out an experiment of 10-hour anesthesia transport under the optimal anesthetic concentrations of MS-222 and eugenol , and the survival rate during the transportation and after 24 h of recovery for the anesthesia group were both 100%, significantly higher than those for the non-anesthetic transport group (P<0.05). The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels increased significantly in the anesthesia transport group compared with those in the non-anesthetic transport group and hydrostatic control group (P<0.05). The ALT and AST levels in the MS-222 anesthesia group were significantly higher than those in the eugenol anesthesia group (P<0.05).