Volume 3 Issue 2
Apr.  2007
Turn off MathJax
Article Contents
Abstract
References
Related Articles
ZHU Caiyan, YE Wei, XIA Junhong, FU Yun, SU Tianfeng, JIANG Shigui. Identification of microsatellite DNA primers in mud carp Cirrhinus molitorella and applicability detection[J]. South China Fisheries Science, 2007, 3(2): 15-19.
Citation: ZHU Caiyan, YE Wei, XIA Junhong, FU Yun, SU Tianfeng, JIANG Shigui. Identification of microsatellite DNA primers in mud carp Cirrhinus molitorella and applicability detection[J]. South China Fisheries Science, 2007, 3(2): 15-19.
shu

Identification of microsatellite DNA primers in mud carp Cirrhinus molitorella and applicability detection

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Original Species Field of C.molitorella of Guangdong Province, Guangzhou 511453, China

More Information
  • Received Date: September 14, 2006
  • Revised Date: October 17, 2006
    Graphical Abstract
    • Abstract

  • A group of wild mud carp samples collected from Xijiang River was amplified by PCR using 14 pairs of microsatellite primers isolated from four species of Cyprinidae. The bands were cloned and then sequenced. The products of primer pairs MFW1, MFW2, MFW15, MFW17, Cc7, Cc11 and Bgon22 contained microsatellite sequences. The primers for loci MFW1, MFW2, and Cc7 were redesigned and named as Cm1, Cm2 and Cm3, respectively. The new primers gave more specific amplification in mud carp. The applicability of primer pairs Cm1, Cm2, Cm3, Cc7, MFW15, MFW17 and Bgon22 on wild mud carp samples from Xijiang River was explored, and the genetic diversity of the wild samples was analyzed. The results showed that the detected numbers of alleles within the samples for each primer pair ranged from 2 to 16. Except for the primer pairs MFW15, Cc11, the amplified profiles of the other five (Cm1, Cm2, Cm3, MFW17 and Bgon22) showed high polymorphism.The range of observed heterozygosities and expected heterozygosities were from 0 to 1 (Ho) and 0 to 0.9038(He) with an average of 0.7772(SD=0.1931)and 0.6881(SD=0.1819), respectively.Significant (P < 0.05)linkage disequilibrium(LD) exhibited in the sample only between locus Cm1 and Bgon22. The average genetic diversity of the samples was 0.6823, showing the level of polymorphism within the samples was high relatively. This study showed that the seven primer pairs of microsatellite locis screened were suitable for genetic diversity analysis of mud carp.

    • FullText(HTML)
    • References (15)
  • [1]
    郑光明, 朱新平, 张跃, 等. 珠江流域不同江段鲮鱼遗传差异研究[J]. 农业生物技术学报, 2001, 9(2): 178-182. doi: 10.3969/j.issn.1674-7968.2001.02.020
    [2]
    朱彩艳, 叶卫, 夏军红, 等. 广东1个鲮原种群体的种质特征及遗传多样性分析[J]. 南方水产, 2005, 1(4): 1-5. doi: 10.3969/j.issn.2095-0780.2005.04.001
    [3]
    HAMADA H, PITRINO M, KAKUAGA T. Anovel reper telement with Z-DNA-forming potentialis widely found inevolutionarily diverse evkaryotic genomes[J]. Pron Nat1 Acad Sci, USA, 1982, 79(2): 6465-6469.
    [4]
    刘志毅, 相建海. 微卫星DNA分子遗传标记在海洋动物遗传分析中的应用[J]. 海洋科学, 2001, 25(6): 11-13. doi: 10.3969/j.issn.1000-3096.2001.06.004
    [5]
    CROOIJMANS R P M A, BIERBOOMS V A F, KOMEN J, et al. Microsatellite markers in common carp(Cyprinus carpio L.)[J]. Animal Genet, 1997, 28(2): 129-134. doi: 10.1111/j.1365-2052.1997.00097.x
    [6]
    NAISH K A, SKIBINSKI D O F. Tetranucleotide microsatellite loci for Indian major carp[J]. Fish Biol, 1998, 53(3): 886-889.
    [7]
    CHENUIL A, GALTIER N, BERREBI P. A test of the hypothesis of an autopolyploid vs. allopolyploid origin of a tetraploid lineage: application of genus Barbus(Cyprinidae)[J]. Heredity, 1999, 82(4): 373-380. doi: 10.1038/sj.hdy.6884890
    [8]
    BY K K Lal, CHAUHAN T, MANDAL A, et al. Identification of microsatellite DNA markers for population structure analysis in Indian major carp, Cirrhinus mrgala(Hamton-Buchanan, 1882)[J]. Appl Ichthyol, 2004, 20(2): 87-91. doi: 10.1046/j.1439-0426.2003.00538.x
    [9]
    YEH Francis C, YANG R C, BOYLE T B J, et al. Popgene, the user-friendly share ware for population genetic analysis[P]. Molecular Biology and Biotechnology Centre, University of Alberta, Canada, 1997. https://www.researchgate.net/publication/292771160_POPGENE_the_user-friendly_shareware_for_population_genetic_analysis_Molecular_Biology_and_Biotechnology_Centre_University_of_Alberta
    [10]
    RAYMOND M, ROUSSET F. Genepop (version 1.2): population genetics software for exact tests and ecumenicism[J]. Heredity, 1995, 86(3): 248-249. doi: 10.1093/oxfordjournals.jhered.a111573
    [11]
    江世贵, 张殿昌, 吕俊霖, 等. 3种野鲮亚科鱼类16S rRNA基因序列分析[J]. 南方水产, 2005, 1(1): 1-5. doi: 10.3969/j.issn.2095-0780.2005.01.001
    [12]
    MADELEINE J H O, HANS K, MARTIN G. Assessing the limits of RAPDs in seaweed biogeography[J]. Phycol, 1996, 32(1): 433-444. https://www.cnki.com.cn/Article/CJFDTotal-NFSC200501002.htm
    [13]
    张小谷, 童金苟, 熊邦喜. 微卫星标记在鱼类遗传及育种研究中的应用[J]. 农业生物技术学报, 2006, 14(1): 117-121. doi: 10.3969/j.issn.1674-7968.2006.01.023
    [14]
    ZANE L, BARGELLONI L, PATARNELLO T. Invited review: Strategies for microsatellite isolation: a review[J]. Mol Ecol, 2002, 11(1): 1-16. doi: 10.1046/j.0962-1083.2001.01418.x
    [15]
    周莉, 刘静霞, 桂建芳, 等. 应用微卫星标记对雌核发育银鲫的遗传多样性初探[J]. 动物学研究, 2001, 22(4): 257-264. doi: 10.3321/j.issn:0254-5853.2001.04.001
    • Related Articles

  • Related Articles

    [1]PENG Ye, LI Jie, WANG Tao, ZHANG Kai, NING Xianhui, JI Jie, YIN Shaowu. Preliminary study on distribution characteristics and positioning of microsatellites in whole genome of Pelteobagrus vachelli[J]. South China Fisheries Science, 2022, 18(1): 90-98. DOI: 10.12131/20210168
    [2]HU Yuting, JIANG He, DUAN Guoqing, ZHOU Huaxing, LING Jun, WANG Huan. Genetic diversity analysis of Pelteobagrus fulvidraco from two major drainage systems in Anhui Province based on microsatellite markers[J]. South China Fisheries Science, 2020, 16(5): 33-41. DOI: 10.12131/20200063
    [3]TANG Shoujie, BI Xiang, WANG Chenghui, ZHANG Feiming, ZHANG Youliang, XIE Zhiqiang. Genetic potential analysis of three selective breeding populations of blunt snout bream (Megalobrama amblycephala) using microsatellite markers[J]. South China Fisheries Science, 2017, 13(2): 59-68. DOI: 10.3969/j.issn.2095-0780.2017.02.008
    [4]ZHU Bing, FAN Jiajia, BAI Junjie, JIANG Peng. Gold grass carp microsatellite polymorphism and its comparative analysis with four grass carp populations from China[J]. South China Fisheries Science, 2017, 13(2): 51-58. DOI: 10.3969/j.issn.2095-0780.2017.02.007
    [5]YANG Bing, LIN Lin, LI Chunhou, XU Shannan, LIU Yong, XIAO Yayuan, CHEN Zuozhi. Development and evaluation of microsatellite markers in Parargyrops edita[J]. South China Fisheries Science, 2015, 11(4): 116-120. DOI: 10.3969/j.issn.2095-0780.2015.04.017
    [6]SUN Chengfei, YE Xing, DONG Junjian, TIAN Yuanyuan, LIANG Jianhui. Genetic diversity analysis of six cultured populations of Macrobrachium rosenbergii using microsatellite markers[J]. South China Fisheries Science, 2015, 11(2): 20-26. DOI: 10.3969/j.issn.2095-0780.2015.02.003
    [7]LIU Wei, SU Shengyan, DONG Zaijie, ZHANG Jianqiao, MA Liangxiao, LI Lingling, QV Jiangqi, YUAN Xinhua. Correlation analysis of microsatellite DNA markers with growth trait among 3 breeding populations of common carp[J]. South China Fisheries Science, 2012, 8(3): 17-24. DOI: 10.3969/j.issn.2095-0780.2012.03.003
    [8]ZHU Lijing, SUN Zhongxiang, CHEN Shuyin, JI Hongjiu, XU Xiaofeng, SHEN Songdong. Microsatellite enrichment by magnetic beads in Coelomactra antiquate[J]. South China Fisheries Science, 2011, 7(4): 10-15. DOI: 10.3969/j.issn.2095-0780.2011.04.002
    [9]XIA Jun-hong, ZHU Cai-yan, SU Tian-feng, ZHOU Fa-lin, JIANG Shi-gui. Isolation and characterization of microsatellites from the tiger shrimp Penaeus monodon[J]. South China Fisheries Science, 2006, 2(6): 1-7.
    [10]ZHU Cai-yan, YE Wei, XIA Jun-hong, FU Yun, ZHOU Fa-lin, JIANG Shi-gui. Germplasm characteristics and genetic diversity analysis of an original species population of mud carp (Cirrhinus molitorella) from Guangdong Province[J]. South China Fisheries Science, 2005, 1(4): 1-5.

Catalog

    Get Citation
    PDF
    XML
    Article views (5354) PDF downloads (3608) Cited by()
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return