XIA Junhong. Identification standard of Sparid species based on cytochrome b sequences[J]. South China Fisheries Science, 2007, 3(1): 37-43.
Citation: XIA Junhong. Identification standard of Sparid species based on cytochrome b sequences[J]. South China Fisheries Science, 2007, 3(1): 37-43.

Identification standard of Sparid species based on cytochrome b sequences

More Information
  • Received Date: August 14, 2006
  • Revised Date: August 27, 2006
  • Twenty-four Sparid cytochrome (Cyt) b sequences belonging to 10 genus, 12 species were downloaded from the molecular biology database NCBI. Of the 1 070 bp consensus sequences obtained by alignment, 675 sites were monophic, 393 were variable sites, 2 were missing sites, and in which no gap was detected. The species-specific sites ranged from 4 to 24 with an average of 12.42 for each species. The distribution range for the interspecies genetic distances was from 0.105 0.010(SE) to 0.220 0.017(SE), and the mean value for that was 0.180±0.0231(SD), whereas all of the intraspecies genetic distances were equal to or less than 0.018±0.004(SE), and the mean value was 0.007±0.006(SD).The genetic distances between species therefore were far more than those within species. Furthermore, phylogenetic analysis also indicated that all the sequences from the same species were clustered to one group. Such result suggested that the Sparid species could be distinguished clearly using Cyt b sequences. Based on this examination of the reference sequences, we provisionally suggested that the genetic distance less than 0.018 between 2 Sparid individuals indicates positive species identification, whereas more than 0.105 indicates negative species identification. This study provides some guidelines for the molecular species identification of the Sparidae.

  • [1]
    ORRELL T M, CARPENTER K E. A phylogeny of the fish family Sparidae (porgies) inferred from mitochondrial sequence data[J]. Mol Phylogenet Evln, 2004, 32(2): 425-434. doi: 10.1016/j.ympev.2004.01.012
    [2]
    孟庆闻, 苏锦祥, 廖学祖. 鱼类分类学[M]. 北京: 中国农业出版社, 1997: 701-709.
    [3]
    ALARCóN J A, ALVAREZ M C. Genetic identification of sparid species by isozyme markers: application to interspecific hybrids[J]. Aquac, 1999, 173(3): 95-103. doi: 10.1016/S0044-8486(98)00473-6
    [4]
    HANEL R, STURMBAUER C. Multiple recurrent evolution of trophic types in northeastern Atlantic and Mediterranean seabreams(Sparidae, Percoidei)[J]. J Mol Evln, 2000, 50(3): 276-283. doi: 10.1007/s002399910032
    [5]
    刘红艳, 苏天凤, 周发林, 等. 四种鲷科鱼类内细胞色素b基因序列的保守性[J]. 湛江海洋大学学报, 2003, 23(1): 8-12.
    [6]
    WHITMORE D H, THAI T H, CRAFT C M. The largemouth bass cytochrome b gene[J]. J Fish Biol, 1994, 44(4): 637-645. doi: 10.1111/j.1095-8649.1994.tb01240.x
    [7]
    JEFFS P. Multiple sequence alignment with Clustal X[J]. Computer Corner, 1998, 23(10): 78-80. https://pubmed.ncbi.nlm.nih.gov/9810230/
    [8]
    KUMAR S, TAMURA K, JAKOBSEN I B, et al. MEGA2: Molecular evolutionary genetics analysis software[J]. Bioinformatics, 2001, 17(12): 1244-1245. doi: 10.1093/bioinformatics/17.12.1244
    [9]
    ROZAS J, SNCHEZ-DELBARRIO J C, MESSEGUER X, et al. DnaSP, DNA polymorphism analyses by the coalescent and other methods[J]. Bioinformatics, 2003, 19(18): 2496-2497. doi: 10.1093/bioinformatics/btg359
    [10]
    HENSHAW M D, LEDUC R G, CHIVERS S J, et al. Identifying beaked whales (family ziphiidae) using mtDNA sequences[J]. Mar Mammal Sci, 1997, 13(3): 487-495. doi: 10.1111/j.1748-7692.1997.tb00656.x
    [11]
    DALEBOUT M L, MEAD J G, BAKER C S, et al. A new species of beaked whale Mesoplodon perrini SP. N. (Cetacea: ziphiidae) discovered through phylogenetic analyses of mitochondrial DNA sequences[J]. Mar Mammal Sci, 2002, 18(3): 577-608. doi: 10.1111/j.1748-7692.2002.tb01061.x
  • Related Articles

    [1]HUANG Xiaoshuai, XU Yu, HU Xiaojuan, XU Wujie, SU Haochang, WEN Guoliang, YANG Keng, CAO Yucheng. Genetic diversity analysis of first filial generation of seven introduced Litopenaeus vannamei populations using microsatellite DNA markers[J]. South China Fisheries Science, 2019, 15(1): 54-62. DOI: 10.12131/20180135
    [2]ZHANG Jiachen, LIU Jianyong, YUAN Ruipeng, HU Zhiguo. Effect of stocking density on growth and survival of inbred and hybrid offspring of Litopenaeus vannamei[J]. South China Fisheries Science, 2015, 11(4): 53-58. DOI: 10.3969/j.issn.2095-0780.2015.04.008
    [3]CHEN Xinghan, GUO Liang, LI Mingming, MENG Zining, LIN Haoran. Analysis of RAPD and mitochondrial Cyt b gene sequences of three cultured stocks of Epinephelus coioides from Guangdong Province[J]. South China Fisheries Science, 2014, 10(4): 27-33. DOI: 10.3969/j.issn.2095-0780.2014.04.005
    [4]ZHANG Huajun, LI Zhuojia, ZHANG Jiasong, ZHANG Xiaoyang, CAO Yucheng, WEN Guoliang, CHENG Kaimin. Effects of stocking density on immune parameters and growth of juvenile Litopenaeus vannamei[J]. South China Fisheries Science, 2012, 8(4): 43-48. DOI: 10.3969/j.issn.2095-0780.2012.04.007
    [5]LI Zhuojia, LI Shuohan, YANG Yingying, WEN Guoliang, LI Yiwen, YANG Yufeng. Bacteria variation in Litopenaeus vannamei high level ponds and its relation with physical-chemical factors[J]. South China Fisheries Science, 2010, 6(4): 6-12. DOI: 10.3969/j.issn.1673-2227.2010.04.002
    [6]LIU Xiaozhu, LI Zhuojia, CAO Yucheng, WEN Guoliang. Common species composition, quantity variation and dominant species of planktonic microalgae in low salinity culture ponds[J]. South China Fisheries Science, 2009, 5(1): 9-16. DOI: 10.3969/j.issn.1673-2227.2009.01.002
    [7]FU Yun, ZHONG Jinxiang, XIE Xiaoyong, YE Wei, LIN Bihai, CHEN Huichong, ZHANG Hanhua. Genetic diversity and specific AFLP bands in three cultured tilapia strains[J]. South China Fisheries Science, 2008, 4(6): 50-55.
    [8]XIE Xiaoyong, SU Tianfeng, CHEN Wen, ZHANG Zhi, YAN Yuanyi, JIANG Shigui. Analysis on genetic diversity of six cultured stocks of Litopenaeus vannamei[J]. South China Fisheries Science, 2008, 4(6): 42-49.
    [9]CHEN Yancui, GAO Tianxiang, CHEN Siqing, ZHUANG Zhimeng, LIU Zhanglin. Isozyme analysis of a cultured Platichthys stellatus stock[J]. South China Fisheries Science, 2008, 4(1): 48-52.
    [10]DU Bo, GONG Shiyuan, TONG Xin, HUANG Guiju, YU Dahui. Genetic variation of hatchery-produced populations of Haliotis discus hannai and H.discus discus in southern China based on microsatellite DNA[J]. South China Fisheries Science, 2007, 3(6): 22-29.

Catalog

    Article views PDF downloads Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return