Volume 3 Issue 1
Feb.  2007
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MU Xidong, BAI Junjie, YE Xing, WANG Xuejie, HU Yinchang. Effects of exogenous estradiol-17β on pituitary-thyroid axis of Carassius auratus[J]. South China Fisheries Science, 2007, 3(1): 31-36.
Citation: MU Xidong, BAI Junjie, YE Xing, WANG Xuejie, HU Yinchang. Effects of exogenous estradiol-17β on pituitary-thyroid axis of Carassius auratus[J]. South China Fisheries Science, 2007, 3(1): 31-36.
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Effects of exogenous estradiol-17β on pituitary-thyroid axis of Carassius auratus

  • College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China

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  • Received Date: October 31, 2006
  • Revised Date: November 06, 2006
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    • Abstract

  • In this study, SYBR Green Ⅰ fluorescence quantitative reverse transcription polymerase chain reaction and radioimmunoassay (RIA) methods were used to investigate the effects of exogenous estradiol-17β (E2) on pituitary-thyroid axis of Carassius auratus. The study was carried out in 10 weeks during which E2 was injected every two weeks for experiment groups at a dose of 0.5 mg per kilogram body weight (resolved in 57% ethanol), while the same dose of 57% ethanol was injected for control groups. The fish were not fed throughout the experimental period. The results showed that compared with control group, thyroid-stimulating hormone β subunit mRNA levels did not have significant changes after both 3 and 5 times of E2 injection, but have an increasing trend. On the other hand, RIA showed that after 3 times of E2 injection, thyroid hormone T3 and T4 concentration levels increased significantly compared with control group (P < 0.05); while after 5 times of E2 injection, T3 and T4 levels showed extremely significant differences (P < 0.01). All of the above demonstrated that exogenous E2 could influence the normal physiological functions of C. auratus.

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    • References (26)
  • [1]
    LAM T J, SHARMA R. Effects of salinity and thyroxine on larval survival, growth and development in the carp, Cyprinus carpio[J]. Aquac, 1985, 44(3): 201-212. doi: 10.1016/0044-8486(85)90244-3
    [2]
    PLISETSKAYA E, WOO N Y S, MURAT J C. Thyroid hormones in cyclostomes and fish and their role in regulation of intermediary metabolism[J]. Comp Biochem Physiol A, 1983, 74(2): 179-187. doi: 10.1016/0300-9629(83)90586-8
    [3]
    TAGAWA M, OGASAWARA T, SAKAMOTO T, et al. Thyrod hormone concentration in the gonads of wild chum salmon during maturation[J]. Fish Physiol Biochem, 1994, 13(3): 233-240. doi: 10.1007/BF00004361
    [4]
    MARTIN S A M, WALLNER W, YOUNGSON A F, et al. Differental expression of Atlantic salmon thyrotropin β subunit mRNA and its cDNA sequence[J]. J Fish Biol, 1999, 54(4): 756-766. doi: 10.1111/j.1095-8649.1999.tb02031.x
    [5]
    赵维信. 鱼类生理学[M]. 北京: 高等教育出版社, 1993: 190.
    [6]
    KORSGAARD E B, ETERSEN I M. Natural occurrence, and experimental induction by estradiol-17β of a lipophosphoprotein (vitellogenin) in flounder (Platichthys flesus L.)[J]. Comp Biochem Physiol B, 1976, 54(4): 443-446. https://pubmed.ncbi.nlm.nih.gov/939084/
    [7]
    ELLIOTT J A K, BROMAGE N R, WHITEHEAD C. Effects of estradiol-17 on serum calcium and vitellogenin levels in rainbow trout[J]. Endocrinol, 1979, 83(1): 54-55.
    [8]
    PIFERRER F, DONALDSON E M. Uptake and clearance of exogenous estradiol-17 beta and testosterone during the early development of coho salmon (Oncorhynchus kisutch), including eggs, alevins and fry[J]. Fish Physiol Biochem, 1994, 13(3): 219-232. doi: 10.1007/BF00004360
    [9]
    PAPOULIAS D M, NOLTIE D B, TILLITT D E. An in vivo model fish system to test chemical effects on sexual differentiation and development: exposure to ethinyl estradiol[J]. Aquat Toxicol, 2000, 48(1): 37-50. doi: 10.1016/S0166-445X(99)00026-0
    [10]
    KRISFALUSI M, EROSCHENKO V P, CLOUD J G. Methoxychlor and estradiol-17 beta affect alevin rainbow trout (Oncorhynchus mykiss) mortality, growth, and pigmention[J]. Bull Environ Contan Toxicol, 1998, 61(4): 519-526. doi: 10.1007/s001289900793
    [11]
    HOU Y Y, SUZUKI Y, AIDA K. Effects of steroid hormones on immunoglobulin M (IgM) in rainbow trout Oncorhynchus mykiss[J]. Fish Physiol Biochem, 1999, 20(2): 155-162. doi: 10.1023/A:1007799617597
    [12]
    PERSSON P, JOHANNSSON S H, TAKAGI Y, et al. Estradiol-17 beta and nutritional status affect calcium balance, scale and bone resorption, and bone formation in rainbow trout, Oncorhynchus mykiss[J]. Comp Physiol, 1997, 167(7): 468-473. doi: 10.1007/s003600050098
    [13]
    KOBAYASHI H, IWAMATSU T. Sex reversal in the medaka Oryzias latipes by brief exposure of early embryos to estradiol-17 beta[J]. Zool Sci, 2005, 22(10): 1163-1167. doi: 10.2108/zsj.22.1163
    [14]
    BJOERNSSON B T, HAUX C, BERN H A, et al. 17 beta-estradiol increases plasma calcitonin levels in salmonid fish[J]. Endocrinology, 1989, 125(4): 1754-1760. doi: 10.1210/endo-125-4-1754
    [15]
    BEGAY V, VALOTAIRE Y, RAVAULT J P, et al. Detection of estrogen receptor mRNA in trout pineal and retina: estrodiol-17 beta modulates melatonin production by cultured pineal photoreceptor cells[J]. Gen Comp Endocrinol, 1994, 93(1): 61-69. doi: 10.1006/gcen.1994.1008
    [16]
    TYLER C R, JOLBING S, SUMPTER T P. Endocrine disruption in wildlife: A critical review of the evidence[J]. Critical Rev Toxicol, 1998, 28(4): 319-361. doi: 10.1080/10408449891344236
    [17]
    RUDEL R. Predicting health effects of exposure to compounds with estrogenic activity: methodological issues[J]. Environ Health Perspect, 1997, 105(suppl. 3): 655-663. https://pubmed.ncbi.nlm.nih.gov/9168010/
    [18]
    曲宪成, 杨艳红, 刘颖, 等. 异育银鲫促甲状腺激素β亚基基因的克隆及序列分析[J]. 上海水产大学学报, 2006, 15(2): 129-135. http://shhydxxb.ijournals.cn/shhy/article/abstract/20060234
    [19]
    YOUNG G, BALL J N. Effects of ovariectomy and oestrogen treatment on the ultrastructure of the adenohypophysis in the teleost Poecilia latipinna[J]. Endocrinol, 1979, 83(1): 30. https://pubmed.ncbi.nlm.nih.gov/521709/
    [20]
    OLIVEREAU M, LELOUP J, DE LUZE A, et al. Effect of estradiol on the hypophyseal-thyroidal axis of the eel[J]. Gen Comp Endocrinol, 1981, 43(3): 352-363. doi: 10.1016/0016-6480(81)90295-1
    [21]
    QU X, NAGAE M, ADACHI S, et al. Effect of estradiol-17 beta on pituitary-thyroidal axis of Japanese eel (Anguilla japonica)[J]. Acta Oceanol Sin, 2001, 20(4): 585-596. https://www.semanticscholar.org/paper/Effect-of-estradiol-17%CE%B2-on-pituitary-thyroidal-axis-Qu-Xiancheng/7e4c7c71dae1fe917923267a0ecd68c3526ed13f
    [22]
    BANDYOPADHYAY S, BANERJEE P P, BHATTACHARYA S. 17 beta-estradiol releases thyroxine from the thyroid follicles of a teleost, Channa gachua (Ham.)[J]. Gen Comp Endocrinol, 1991, 81(2): 227-233. doi: 10.1016/0016-6480(91)90007-S
    [23]
    LEATHERLAND J F. Studies of the correlation between stress-response, osmoregulation and thyroid physiology in rainbow trout, Salmo gairdneri (Richardson)[J]. Comp Biochem Physiol A, 1985, 80(4): 523-531. doi: 10.1016/0300-9629(85)90407-4
    [24]
    WIENS S C, EALES J G. The effects of 17 beta-estradiol injections on thyroid hormone deiodination pathways in liver and other tissues of female and male rainbow trout (Oncorhynchus mykiss) at different stages of sexual maturity[J]. Rev Can Zool, 2005, 83(4): 596-603. doi: 10.1139/z05-042
    [25]
    张镜如. 生理学[M]. 北京: 人民卫生出版社, 2000: 393-394.
    [26]
    NAGAE M, TODO T, GEN K, et al. Molecular cloning of the cDNAs encoding pituitary glycoprotein hormone α- and gonadotropin Ⅱβ- subunits of the Japanese eel, Anguilla japonica, and increase in their mRNAs during ovarian development induced by injection of chum salmon pituitary homogenate[J]. J Mol Endocrinol, 1996, 16(2): 171-181. https://pubmed.ncbi.nlm.nih.gov/9156520/
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