Volume 4 Issue 6
Dec.  2008
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FU Yun, ZHONG Jinxiang, XIE Xiaoyong, YE Wei, LIN Bihai, CHEN Huichong, ZHANG Hanhua. Genetic diversity and specific AFLP bands in three cultured tilapia strains[J]. South China Fisheries Science, 2008, 4(6): 50-55.
Citation: FU Yun, ZHONG Jinxiang, XIE Xiaoyong, YE Wei, LIN Bihai, CHEN Huichong, ZHANG Hanhua. Genetic diversity and specific AFLP bands in three cultured tilapia strains[J]. South China Fisheries Science, 2008, 4(6): 50-55.
shu

Genetic diversity and specific AFLP bands in three cultured tilapia strains

  • 1.

    Guangdong National Elite Tilapia Farm, Guangzhou 511453, China

  • 2.

    Guangdong Provincial Fishery Technical Extension Center, Guangzhou 510222, China

  • 3.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: June 15, 2008
  • Revised Date: September 02, 2008
    Graphical Abstract
    • Abstract

  • New Gift tilapia, GenoMar tilapia, and the hybrid tilapia of Oreochromis niloticus ♀ ×O.aureus ♂ were three major cultured tilapia lines in south China. Identifying these lines was important especially at fry stage. Using amplified fragment length polymorphism(AFLP)technique, we analyzed a total of 60 samples with five primer pairs. Out of 245 AFLP bands, 53.02% were polymorphic. Seven bands from primer pair E-ACA/M-CAT and two bands from E-ACA/M-CAG were found to be specific and could be used to distinguish the three cultured tilapia lines. As for genetic diversity of the three tilapia lines, gene diversity index of New Gift tilapia, GenoMar tilapia, and the hybrid were 0.1605, 0.1595, and 0.2214, respectively. Percent of polymorphic bands and Shannon index presented the similar trend in gene diversity index in these three lines, suggesting that New Gift tilapia was genetically stable, gene diversity index of GenoMar tilapia was a little small.

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    • References (24)
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