FU Yun, ZHONG Jinxiang, XIE Xiaoyong, YE Wei, LIN Bihai, CHEN Huichong, ZHANG Hanhua. Genetic diversity and specific AFLP bands in three cultured tilapia strains[J]. South China Fisheries Science, 2008, 4(6): 50-55.
Citation: FU Yun, ZHONG Jinxiang, XIE Xiaoyong, YE Wei, LIN Bihai, CHEN Huichong, ZHANG Hanhua. Genetic diversity and specific AFLP bands in three cultured tilapia strains[J]. South China Fisheries Science, 2008, 4(6): 50-55.

Genetic diversity and specific AFLP bands in three cultured tilapia strains

More Information
  • Received Date: June 15, 2008
  • Revised Date: September 02, 2008
  • New Gift tilapia, GenoMar tilapia, and the hybrid tilapia of Oreochromis niloticus ♀ ×O.aureus ♂ were three major cultured tilapia lines in south China. Identifying these lines was important especially at fry stage. Using amplified fragment length polymorphism(AFLP)technique, we analyzed a total of 60 samples with five primer pairs. Out of 245 AFLP bands, 53.02% were polymorphic. Seven bands from primer pair E-ACA/M-CAT and two bands from E-ACA/M-CAG were found to be specific and could be used to distinguish the three cultured tilapia lines. As for genetic diversity of the three tilapia lines, gene diversity index of New Gift tilapia, GenoMar tilapia, and the hybrid were 0.1605, 0.1595, and 0.2214, respectively. Percent of polymorphic bands and Shannon index presented the similar trend in gene diversity index in these three lines, suggesting that New Gift tilapia was genetically stable, gene diversity index of GenoMar tilapia was a little small.

  • [1]
    VOS P, HOGERS R, BLEEKOR M, et al. AFLP a new technique for DNA fingerprinting[J]. Nucleic Res, 1995, 23(21): 4 407-4 414. doi: 10.1093/nar/23.21.4407
    [2]
    张俊彬, 黄良民, 陈真然. AFLP技术在笛鲷的仔鱼鉴定及其分类学上的研究[J]. 海洋学报, 2005, 27(2): 165-171. doi: 10.3321/j.issn:0253-4193.2005.02.022
    [3]
    王志勇, 邱淑贞. 福建官井洋大黄鱼AFLP指纹多态性的研究[J]. 中国水产科学, 2002, 9(3): 198-202. doi: 10.3321/j.issn:1005-8737.2002.03.002
    [4]
    宋君, 宋昭彬, 岳碧松, 等. 长江合江江段岩原鲤种群遗传多样性的AFLP分析[J]. 四川动物, 2005, 24(4): 495-499. doi: 10.3969/j.issn.1000-7083.2005.04.013
    [5]
    LIU Y G, CHEN S L, LI B F, et al. Analysis of genetic variation in selected stocks of hatchery flounder, Paralichthys olivaceus, using AFLP markers[J]. Biochem Systematics Ecol, 2005, 33(10): 993-1 005. doi: 10.1016/j.bse.2005.02.008
    [6]
    FELIP A, MARTINEZ-RODRIGUEZ G, PIFERRER F, et al. AFLP analysis confirms exclusive maternal genomic contribution of meiogynogenetic sea bass (Dicentrarchus labrax L. )[J]. Mar Biotechnol, 2000, 2(3): 301-306. doi: 10.1007/s101260000000
    [7]
    SIMMONS M, MICKETT K, KUCUKTAS H, et al. Comparison of domestic and wild channel catfish (Ictalurus punctatus) populations provides no evidence for genetic impact[J]. Aquac, 2006, 252(2/4): 133-146. doi: 10.1016/j.aquaculture.2005.11.006
    [8]
    KIDD M R, KIDD C E, KOCHER T D. Axes of differentiation in the bower-building cichlids of Lake Malawi[J]. Mol Ecol, 2006, 15 (2): 459-478. doi: 10.1111/j.1365-294X.2005.02787.x
    [9]
    LIU Z J, KARSI A, LI P, et al. An AFLP-based genetic linkage map of channel catfish (Ictalurus punctatus) constructed by using an interspecific hybrid resource family[J]. Genet, 2003, 165(2): 687-694. doi: 10.1093/genetics/165.2.687
    [10]
    NICHOLS K M, YOUNG W P, DANZMANN R G, et al. A consolidated linkage map for rainbow trout (Oncorhynchus mykiss)[J]. Anim Genet, 2003, 34(2): 102-115. doi: 10.1046/j.1365-2052.2003.00957.x
    [11]
    ZIMMERMAN A M, WHEELER P A, RISTOW S S, et al. Composite interval mapping reveals three QTL associated with pyloric caeca number in rainbow trout, Oncorhynchus mykiss[J]. Aquac, 2005, 247(1/4): 85-95. doi: 10.1016/j.aquaculture.2005.02.029
    [12]
    FELIP A, YOUNG W P, WHEELER P A, et al. An AFLP-based approach for the identification of sex-linked markers in rainbow trout (Oncorhynchus mykiss)[J]. Aquac, 2005, 247(1/4): 35-43. doi: 10.1016/j.aquaculture.2005.02.014
    [13]
    AGRESTI J J, SEKI S, CNAANI A, et al. Breeding new strains of tilapia: development of an artificial center of origin and linkage map based on AFLP and microsatellite loci[J]. Aquac, 2000, 185(1/2): 43-56. doi: 10.1016/S0044-8486(99)00335-X
    [14]
    KOCHER T D, LEE W J, SOBOLEWSKA H, et al. A genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus)[J]. Genet, 1998, 148: 1 225-1 232. doi: 10.1093/genetics/148.3.1225
    [15]
    EZAZ M T, HARVEY S C, BOONPHAKDEE C, et al. Isolation and physical mapping of sex-linked AFLP markers in Nile tilapia (Oreochromis niloticus L. ) [J]. Mar Biotechnol, 2004, 6(5): 435-445. doi: 10.1007/s10126-004-3004-6
    [16]
    MOEN T, AGRESTI J J, CNAANI A, et al. A genome scan of a four-way tilapia cross supports the existence of a quantitative trait locus for cold tolerance on linkage group 23[J]. Aquac Res, 2004, 35(9): 893-904. doi: 10.1111/j.1365-2109.2004.01082.x
    [17]
    EZAZ M T, SAYEED S, MCANDREW B J. Use of microsatellite loci and AFLP markers to verify gynogenesis and clonal lines in Nile tilapia Oreochromis niloticus L[J]. Aquac Res, 2004, 35(15): 1 472-1 481. doi: 10.1111/j.1365-2109.2004.01175.x
    [18]
    SAMBROOK J, RUSSELL D W. 分子克隆实验指南[M]. 黄培堂, 金冬雁, 黎孟枫, 等译. 3版. 北京: 科学出版社, 2002: 8.
    [19]
    YEH J C, YANG R, BOYLE T. POPGENE Version 1.31: Microsoft window-based freeware for population genetic analysis[CP]. Edmonton: University of Alberta, 1999. https://xueshu.baidu.com/usercenter/paper/show?paperid=c557481ab723709e6c234f982a00ef01&site=xueshu_se&hitarticle=1
    [20]
    [21]
    [22]
    [23]
    [24]
    李莉好, 喻达辉, 黄桂菊, 等. 吉富罗非鱼不同选育群体的遗传多样性[J]. 南方水产, 2007, 3(5): 40-48. doi: 10.3969/j.issn.2095-0780.2007.05.007
  • Related Articles

    [1]ZHOU Falin, YANG Qibin, JIANG Song, YANG Lishi, LI Yundong, HUANG Jianhua, JIANG Shigui. Analysis of combining ability and heterosis on body mass trait of three Penaeus monodon populations[J]. South China Fisheries Science, 2021, 17(1): 39-44. DOI: 10.12131/20200157
    [2]SUN Minglong, BAI Zhiyi, FU Baicheng, SUN Tianyang. Effects of β-carotene supplement in bait on inner shell color, tissue total carotenoids content and growth of different color strains in Hyriopsis cumingii[J]. South China Fisheries Science, 2020, 16(5): 80-86. DOI: 10.12131/20200075
    [3]WU Shuiqing, ZHENG Leyun, LUO Huiyu, HUANG Zhongchi, LIN Kebing, QIU Fengyan, WU Jingling, LIN Jinbo. Comparison of morphological traits of hybrid groupers (Epinephelus coioides♀×E.akaara♂) and their parents[J]. South China Fisheries Science, 2017, 13(5): 47-54. DOI: 10.3969/j.issn.2095-0780.2017.05.007
    [4]LIN Mingxue, YANG Jie, YUE Wucheng, KANG Wei, LEI Shuangyong, DU Jinxing, WANG Chenghui. Analysis of combining ability, heterosis and genetic correlation on growth traits of GIFT tilapia[J]. South China Fisheries Science, 2016, 12(2): 1-6. DOI: 10.3969/j.issn.2095-0780.2016.02.001
    [5]HU Zhiguo, LIU Jianyong, YUAN Ruipeng, ZHANG Jiachen. Combining ability for resistance of Litopenaeus vannamei to ammonia nitrogen and dissolved oxygen[J]. South China Fisheries Science, 2016, 12(1): 43-49. DOI: 10.3969/j.issn.2095-0780.2016.01.007
    [6]ZOU Keshu, ZHANG Dianchang, GUO Huayang, ZHANG Nan, JIANG Shigui. Comparison of seven metallic elements in prismatic layer and nacreous layer of shells from four shell color strains in pearl oyster (Pinctada fucata)[J]. South China Fisheries Science, 2015, 11(3): 74-79. DOI: 10.3969/j.issn.2095-0780.2015.03.012
    [7]HU Zhiguo, LIU Jianyong, BAO Xiufeng, JIANG Xiang. Combining ability and heterosis of hybridization between cultured and wild stocks of Haliotis diversicolor supertexta[J]. South China Fisheries Science, 2014, 10(1): 43-49. DOI: 10.3969/j.issn.2095-0780.2014.01.007
    [8]LIANG Jian, YAN Xiwu, HUO Zhongming, LIU Zhen, WU Yunxia, YANG Feng. Study on shell length and survival of orange strain of Manila clam (Ruditapes philippinarum) with different inbreeding coefficient[J]. South China Fisheries Science, 2013, 9(4): 87-93. DOI: 10.3969/j.issn.2095-0780.2013.04.015
    [9]LIN Zhuangbing, YOU Weiwei. Heterosis analysis on crossbreeding between different populations of small abalone Haliotis diversicolor[J]. South China Fisheries Science, 2013, 9(4): 28-32. DOI: 10.3969/j.issn.2095-0780.2013.04.005
    [10]JIE Xiaoyong, LI Sifa, CAI Wanqi, YE Wei, CHEN Huichong, YU Dahui. Heterosis analysis of main growth-related traits of tilapia[J]. South China Fisheries Science, 2007, 3(3): 1-7.

Catalog

    Article views (5528) PDF downloads (3424) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return