Volume 2 Issue 6
Dec.  2006
Turn off MathJax
Article Contents
Abstract
References
Related Articles
SHAO Yan-qing, ZHANG Dian-chang, SU Tian-feng, LU: Jun-lin, JIANG Shi-gui. The construction of pituitary gland cDNA library of Nibea coibor[J]. South China Fisheries Science, 2006, 2(6): 8-12.
Citation: SHAO Yan-qing, ZHANG Dian-chang, SU Tian-feng, LU: Jun-lin, JIANG Shi-gui. The construction of pituitary gland cDNA library of Nibea coibor[J]. South China Fisheries Science, 2006, 2(6): 8-12.
shu

The construction of pituitary gland cDNA library of Nibea coibor

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Shanghai Fisheries University, Shanghai 200090, China

More Information
  • Received Date: June 05, 2006
  • Revised Date: September 11, 2006
    Graphical Abstract
    • Abstract

  • A cDNA expression library from the pituitary gland of Nibea coibor was constructed into pcDNA3.1 (+) eukarytical expression plasmid using SMARTTM cDNA library construction protocol. The anchor first strand cDNA containing asymmetrical SfiⅠ restriction enzyme sites (A & B) was synthesized by transcription of total RNA with the SMART technique. The long-distance(LD PCR) was performed using a modified oligo(dT) prime and anchor prime as the prime set, and anchor fist-strand cDNA as the template to enrich the cDNA population for full-length sequences. After digestion with SfiⅠ and size fractionation using CHROM SPIN-400 column, SMART cDNA was ligated into the SfiⅠ-digested pcDNA3.1 (+) -SfiⅠ vector and transferred into the competent cells of Escherichia coli TOP10 strain. The obtained N.coibor cDNA library contains 1.0×105 recombinants, the percentage of vectors with inserts was 90% and the average insert size was between 0.4~3.0 kb. The N.coibor cDNA library gives an ideal base for further study structure and characterization of the gene in relation to growth and development of N.coibor.

    • FullText(HTML)
    • References (9)
  • [1]
    福建鱼类志编写组. 福建鱼类志(下卷)[M]. 福州: 福建科学技术出版社, 1985: 118-119. https://xueshu.baidu.com/usercenter/paper/show?paperid=53a48ef74d12566aabcad12db0e4cdce
    [2]
    沈卓坤, 黄玮, 黄培铃. 浅色黄姑鱼人工繁殖与苗种培育技术[J]. 水产养殖, 2004, 25(5): 6-7. doi: 10.3969/j.issn.1004-2091.2004.05.003
    [3]
    马长艳, 周开亚, 郭豫杰, 等. 中华绒螯蟹卵巢差减cDNA文库的构建[J]. 动物学研究, 2003, 24(1): 53-56. https://xueshu.baidu.com/usercenter/paper/show?paperid=e4ccfb1630b4b4e32cec16bfe2154af9&site=xueshu_se&hitarticle=1
    [4]
    陈慧萍, 姜孝玉, 徐洪滨, 等. 金钱鱼毒腺cDNA表达文库的构建及EST序列分析[J]. 中国生物化学与分子生物学报, 2004, 20(2): 166-170. doi: 10.3969/j.issn.1007-7626.2004.02.005
    [5]
    王艺磊, 张子平. 日本对虾精巢和卵巢全长cDNA文库的构建[J]. 动物学杂志, 2003, 38(2): 9-13. doi: 10.3969/j.issn.0250-3263.2003.02.003
    [6]
    王维新, 史成银, 黄捷. 中国明对虾鳃细胞全长cDNA文库的构建[J]. 海洋水产研究, 2004, 25(5): 6-11. doi: 10.3969/j.issn.1000-7075.2004.05.002
    [7]
    万榕, 宋军, 林旭, 等. 中华眼镜蛇毒腺cDNA表达文库的建立和分析[J]. 第四军医大学学报, 2004, 25(13): 1208-1211. doi: 10.3321/j.issn:1000-2790.2004.13.017
    [8]
    黄英, 张宗玉. cDNA文库构建策略[J]. 国外医学分子生物学分册, 1999, 21(6): 337-340. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg5RSzE5OTkwMDE3NDQzORoINGkxdzkxOTc%3D
    [9]
    傅作申, 程远国, 张玉静, 等. 用长距PCR法构建恶性疟原虫cDNA表达文库[J]. 热带医学杂志, 2002, 2(3): 225-229. doi: 10.3969/j.issn.1672-3619.2002.03.004
    • Related Articles

  • Related Articles

    [1]DAI Yulai, WANG Yuxi, XU Baoqing, DAI Yangxin, LIN Qicun, CAI Lijuan. Study on acute toxicity and histopathology of copper sulfate, trichlorfon and povidone-iodine to GIFT tilapia (Oreochromis niloticus)[J]. South China Fisheries Science, 2023, 19(6): 116-126. DOI: 10.12131/20230093
    [2]HUANG Nanjian, CHEN Haigang, ZHANG Linbao, MA Shengwei, ZHANG Zhe, CAI Wengui, JIA Xiaoping. Acute toxicity of South China Sea LH crude oil and NO.0 diesel to three mariculture and multiplication organisms[J]. South China Fisheries Science, 2015, 11(2): 72-78. DOI: 10.3969/j.issn.2095-0780.2015.02.010
    [3]ZHANG Xincheng, ZHU Xinping, LIU Yihui, HONG Xiaoyou, HUANG Jiancheng, ZENG Minling. Acute toxicity of mebendazole, deltamethrin and copper sulphate to Alosa sapidissima[J]. South China Fisheries Science, 2015, 11(2): 66-71. DOI: 10.3969/j.issn.2095-0780.2015.02.009
    [4]LIU Tianhong, YU Xiaoqing, GUO Pingping, WANG Zhigang, WU Zhihong, SUN Yuanqin, SUN Fuxin, WANG Juan, MA Danping. Effect of different copper salts on acute toxicity and histomorphology of juvenile sea cucumbers[J]. South China Fisheries Science, 2014, 10(6): 58-65. DOI: 10.3969/j.issn.2095-0780.2014.06.008
    [5]YE Le, ZHAO Wang, WU Kaichang. Acute toxicity tests of three antiparasitic drugs to juvenile snail of Babylonia areolata[J]. South China Fisheries Science, 2014, 10(4): 22-26. DOI: 10.3969/j.issn.2095-0780.2014.04.004
    [6]ZHENG Weigang, LI Zhongbao, LI Wenjing, WU Ning. Acute toxicity of Hg2+ to 5 species of genus Anguilla elver[J]. South China Fisheries Science, 2011, 7(4): 16-23. DOI: 10.3969/j.issn.2095-0780.2011.04.003
    [7]SHI Gang, CHEN Gang, ZHANG Jiandong, HUANG Jiansheng, PAN Chuanhao, BAI Lirong. Acute toxicity of 4 aquatic medicines to Lutjanus rivulatus larva[J]. South China Fisheries Science, 2011, 7(3): 50-55. DOI: 10.3969/j.issn.2095-0780.2011.03.009
    [8]WU Xuan, YU Gang, BAI Dong-qing, ZHOU Wen-li. Acute toxic effects of four Chinese herb additives on white cloud mountain fish Tanichthrs altbonubes[J]. South China Fisheries Science, 2010, 6(2): 73-76. DOI: 10.3969/j.issn.1673-2227.2010.02.012
    [9]LI Wenjing, LI Zhongbao, ZHENG Weigang, WU Ning. Acute toxicity of phentriazophos on five species of genus Anguilla elver[J]. South China Fisheries Science, 2009, 5(6): 13-18. DOI: 10.3969/j.issn.1673-2227.2009.06.003
    [10]CHEN Jing, LIU Zhigang, LI Luoying, HE Yongchan. Acute toxicity of three drugs acting on nymph of Sipunculus nudus[J]. South China Fisheries Science, 2009, 5(1): 54-58. DOI: 10.3969/j.issn.1673-2227.2009.01.009

Catalog

    Get Citation
    PDF
    XML
    Article views (5324) PDF downloads (3340) Cited by()
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return