CHENG Fei, YE Wei, YE Fuliang, LIU Bo. The establishment of AFLP analysis system in research of armorhead catfish, Cranoglanis bouderius[J]. South China Fisheries Science, 2007, 3(4): 46-51.
Citation: CHENG Fei, YE Wei, YE Fuliang, LIU Bo. The establishment of AFLP analysis system in research of armorhead catfish, Cranoglanis bouderius[J]. South China Fisheries Science, 2007, 3(4): 46-51.

The establishment of AFLP analysis system in research of armorhead catfish, Cranoglanis bouderius

More Information
  • Received Date: March 21, 2007
  • Revised Date: April 11, 2007
  • AFLP (amplified fragment length polymorphism) analysis system was established in research of Pearl River armorhead catfish, Cranoglanis bouderius. The several steps for AFLP analysis were examined, including DNA extraction, double-restriction endonuclease reaction, single-restriction endonuclease reaction, adadpter ligation reaction, preamplification reaction, selective amplification reaction and Ag-NORs reaction. The AFLP fingerprinting experiments of C.bouderius with primer combination, E+AAG/M+CTG and P+ATT/T+ACA, were performed, which had about 50 clear and bright amplification bands.

  • [1]
    周立斌, 刘晓春, 叶卫, 等. 17β-雌二醇和甲基睾酮对离体长臀脑垂体促性腺激素分泌的影响[J]. 中国水产科学, 2005, 12(2): 113-118. doi: 10.3321/j.issn:1005-8737.2005.02.001
    [2]
    乐佩琦, 陈宜瑜. 中国濒危动物红皮书-鱼类[M]. 北京: 科学出版社, 1998: 13-19. http://ir.ihb.ac.cn/handle/342005/10428
    [3]
    ZEBEAU M, VOS R. Selective estriction fragment amplification: a general method for DNA finger printing[P]. European Patent Application 94202629. 7 Paris: European Patent Office, 1993. https://xueshu.baidu.com/usercenter/paper/show?paperid=58aa828824f0e6bca48f4f5f573f0820
    [4]
    万翔, 刘富中, 宋明, 等. 茄子AFLP技术体系的优化与建立[J]. 西南农业大学学报: 自然科学版, 2005, 12(2): 226-229. doi: 10.3969/j.issn.1673-9868.2005.02.021
    [5]
    许绍斌, 陶玉芬, 杨昭庆, 等. 简单快速的DNA银染和胶保存方法[J]. 遗传, 2002, 24(3): 335-336. doi: 10.3321/j.issn:0253-9772.2002.03.027
    [6]
    张桂华, 杜胜利. 黄瓜AFLP反应体系的建立[J]. 华北农学报, 2004, 19(2): 10-12. https://cpfd.cnki.com.cn/Article/CPFDTOTAL-EGYP201005001018.htm
    [7]
    王赞, 高洪文孙, 韩建国. 柠条锦鸡儿DNA提取及AFLP反应体系的建立[J]. 草地学报, 2005, 13(2): 126-129. doi: 10.11733/j.issn.1007-0435.2005.02.009
    [8]
    王文江, 张桂霞, 崔惠英, 等. 柿AFLP银染技术体系的建立[J]. 河北农业大学学报, 2005, 28(2): 9-14. doi: 10.3969/j.issn.1000-1573.2005.02.003
    [9]
    蔡健, 陆娟, 兰伟. 小麦基因组AFLP技术体系建立的研究[J]. 阜阳师范学院学报: 自然科学版, 2005, 22(2): 25-27. doi: 10.3969/j.issn.1004-4329.2005.02.008
    [10]
    李升康, 熊远著, 邓昌彦. 家猪AFLP分析体系的建立[J]. 华中农业大学学报, 2003, 22(2): 110-113. doi: 10.3321/j.issn:1000-2421.2003.02.003
    [11]
    吴俊, 束怀瑞, 张开春, 等. 桃AFLP技术体系的优化及集群分离分析研究[J]. 西北植物学报, 2005, 25(3): 430-435. doi: 10.3321/j.issn:1000-4025.2005.03.002
    [12]
    李珊, 赵桂. 仿AFLP分子标记及其应用[J]. 西北植物学报, 2003, 23(5): 830-836. doi: 10.3321/j.issn:1000-4025.2003.05.025
    [13]
    贾璇, 杨文香, 刘大群. 小麦基因组AFLP反应体系的建立[J]. 河北农业大学学报, 2003, 23(2): 55-59. doi: 10.3969/j.issn.1000-1573.2003.02.015
  • Related Articles

    [1]HOU Diyao, LI Aoxue, LYU Shaoliang, DONG Jianyu, CHEN Ning, WANG Xuefeng. Analysis of phytoplankton community structure and its relationship with environmental factors in coastal waters of eastern Leizhou Peninsula during spring and autumn[J]. South China Fisheries Science, 2025, 21(1): 55-65. DOI: 10.12131/20240183
    [2]SU Li, XU Shannan, LI Chunhou, CHEN Zuozhi. Phytoplankton community composition and its environmental impact factors in Pearl River Estuary during wet season[J]. South China Fisheries Science, 2025, 21(1): 46-54. DOI: 10.12131/20240143
    [3]ZHU Shuli, CHEN Weitao, WU Zhi, XIA Yuguo, YANG Jiping, LI Yuefei, LI Jie. Preliminary investigation of fish diversity in middle and lower reaches of Pearl River based on environmental DNA technology[J]. South China Fisheries Science, 2024, 20(1): 120-129. DOI: 10.12131/20230111
    [4]CHEN Liangdong, ZHAN Jianpo, WANG Qing. Community structure of phytoplankton and their indicative effect on water quality of Pearl River[J]. South China Fisheries Science, 2023, 19(6): 1-10. DOI: 10.12131/20230059
    [5]CHEN Weitao, DUAN Xinbin, GAO Lei, LI Xinhui, YANG Jiping, WANG Dengqiang. Genetic structure analysis of Ochetobius elongatus between Yangtze River and Pearl River using multiple loci[J]. South China Fisheries Science, 2022, 18(6): 19-25. DOI: 10.12131/20220007
    [6]SHUAI Fangmin, LI Zhiquan, LIU Guowen, LI Xinhui, LI Yuefei, YANG Jiping, LI Jie. Resource status of Japanese eel (Anguilla japonica) in the Pearl River Estuary[J]. South China Fisheries Science, 2015, 11(2): 85-89. DOI: 10.3969/j.issn.2095-0780.2015.02.012
    [7]WU Zhi, TAN Xichang, LI Xinhui, TANG Yong. Acoustic monitoring on fish resources in Xijiang section of Pearl River during first closed fishing season[J]. South China Fisheries Science, 2014, 10(3): 24-28. DOI: 10.3969/j.issn.2095-0780.2014.03.004
    [8]LI Jie, LI Xin-hui. A new record of bagridae fishes in Pearl River water system[J]. South China Fisheries Science, 2008, 4(4): 64-66.
    [9]WANG Di, LIN Zhaojin. Spatial and temporal variations of fish community structure in the Pearl River Estuary waters[J]. South China Fisheries Science, 2006, 2(4): 37-45.
    [10]YANG Lin, ZHANG Xufeng, ZHANG Peng, TAN Yongguang. Composition of by-catch of shrimping beam trawl in the Pearl River Estuary, China[J]. South China Fisheries Science, 2005, 1(1): 27-34.

Catalog

    Article views (5647) PDF downloads (2908) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return