Volume 4 Issue 3
Jun.  2008
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REN Jie, LIN Weitie, LUO Xiaochun, XIE Mingquan. Statistical optimization of fermentation media for nitrite oxidizing bacteria[J]. South China Fisheries Science, 2008, 4(3): 7-14.
Citation: REN Jie, LIN Weitie, LUO Xiaochun, XIE Mingquan. Statistical optimization of fermentation media for nitrite oxidizing bacteria[J]. South China Fisheries Science, 2008, 4(3): 7-14.
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Statistical optimization of fermentation media for nitrite oxidizing bacteria

  • College of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, China

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  • Author Bio:

    REN Jie (1977-), male, Ph.D.candidate, mainly researches on industrial microbe and enviromental microbe. E-mail: renjiescut2001@163.com

  • Corresponding author:

    LIN Weitie, E-mail: 13902202933@139.com

  • Received Date: February 27, 2008
  • Revised Date: March 13, 2008
    Graphical Abstract
    • Abstract

  • The statistical experimental design (Plackett-Burman and Box-Behnken design) was applied to optimize the culture medium of nitrite oxidizing bacteria for improving the nitrite oxidizing rate. Estimated optimum medium composition of the nitrite oxidizing rate was as follows: NaHCO3 2.0 g · L-1; NaNO2 2.36 g · L-1; Na2CO3 0.37 g · L-1; NaCl 0.34 g ·L-1; KH2PO4 0.05 g · L-1; MgSO4 · 7H2O 0.05 g · L-1; and FeSO4 · 7H2O 0.03 g · L-1. The nitrite oxidizing rate reached a maximum at 905.0 mg NO2-N · (g MLSS · d)-1(mixed liquor suspended solids, MLSS).In the field trial, 50 L of nitrite oxidizing bacteria concentrate (1.99 g VSS · L-1)(volatile solid, VSS) with 850 mg NO2-N · (g MLSS · d)-1 were added to 0.6 hectares of the aquaculture water. Nitrite level in all treated ponds remained very low compared to the steady increase observed in all of the control ponds during 7 days.

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    • References (13)
  • [1]
    FRANCES J, ALLAN G L, NOWAK B F. The effects of nitrite on the shortterm growth of silver perch (Bidyanus bidyanus) [J]. Aquac, 1998, 163(1/2): 63-72. doi: 10.1016/S0044-8486(98)00219-1
    [2]
    SHAN H, OBBARD J P. Ammonia removal from prawn aquaculture water using immobilized nitrifying bacteria[J]. Appl Microbiol Biotechnol, 2001, 57(17): 791-798. doi: 10.1007/s00253-001-0835-1
    [3]
    任杰, 林炜铁, 罗小春, 等. 硝化菌保藏特性及衰减动力学研究[J]. 中国生物工程杂志, 2007, 27(12): 61-65. doi: 10.3969/j.issn.1671-8135.2007.12.013
    [4]
    李卓佳, 郭志勋, 冯娟, 等. 应用芽孢杆菌调控虾池微生态的初步研究[J]. 海洋科学, 2006, 30(11): 28-31. doi: 10.3969/j.issn.1000-3096.2006.11.007
    [5]
    李卓佳, 林亮, 杨莺莺, 等. 芽孢杆菌制剂对凡纳滨对虾Litopenaeus vannamei肠道微生物群落的影响[J]. 南方水产, 2005, 1(3): 54-59. doi: 10.3969/j.issn.2095-0780.2005.03.009
    [6]
    林影, 张聚宝, 向柱方. 假丝酵母Candida sp. 木糖发酵生产乙醇[J]. 华南理工大学学报: 自然科学版, 2006, 34(5): 33-37. doi: 10.3321/j.issn:1000-565X.2006.05.007
    [7]
    ANBU P, GOPINATH S C B, HILDA A, et al. Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis[J]. Bioresour Technol, 2006, 98(6): 1 298-1 303. doi: 10.1016/j.biortech.2006.05.047
    [8]
    MAO Xiangzhao, SHEN Yaling, YANG Liang, et al. Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods[J]. Process Biochem, 2007, 42(5): 878-883. doi: 10.1016/j.procbio.2007.01.004
    [9]
    JOHN R P, SUKUMARAN R K, NAMPOOTHIRI M K, et al. Statistical optimization of simultaneous saccharification and L (+)lactic acid fermentation from cassava bagasse using mixed culture of lactobacilli by response surface methodology[J]. Biochem Eng J, 2007, 36(3): 262-267. doi: 10.1016/j.bej.2007.02.028
    [10]
    ISAR J, AGARWAL L, SARAN S, et al. A statistical method for enhancing the production of succinic acid from Escherichia coli under anaerobic conditions[J]. Bioresour Technol, 2006, 97(13): 1 443-1 448. doi: 10.1016/j.biortech.2005.07.014
    [11]
    WILEN B M, JIN B, LANT P. The influence of key chemical constituents in activated sludge on surface and flocculating properties[J]. Water Res, 2003, 37(9): 2 127-2 139. doi: 10.1016/S0043-1354(02)00629-2
    [12]
    SALEM S, MOUSSA M S, VAN LOOSDRECHT M C. Determination of the decay rate of nitrifying bacteria[J]. Biotechnol Bioeng, 2006, 94(2): 252-262. doi: 10.1002/bit.20822
    [13]
    GROMMEN R, VAN HAUTEGHEM I, VAN WAMBEKE, et al. An improved nitrifying enrichment to remove ammonium and nitrite from freshwater aquaria systems[J]. Aquac, 2002, 211(1/4): 115-124. doi: 10.1016/S0044-8486(01)00883-3
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