YU Dahui, WANG Xiaoyu, GUO Yihui, HUANG Guiju, WANG Aimin. Segregation of RAPD markers in F1 generation of Pinctada fucata family[J]. South China Fisheries Science, 2005, 1(6): 1-7.
Citation: YU Dahui, WANG Xiaoyu, GUO Yihui, HUANG Guiju, WANG Aimin. Segregation of RAPD markers in F1 generation of Pinctada fucata family[J]. South China Fisheries Science, 2005, 1(6): 1-7.

Segregation of RAPD markers in F1 generation of Pinctada fucata family

More Information
  • Received Date: August 09, 2005
  • Revised Date: August 21, 2005
  • The mode of segregation of random amplified polymorphic DNA (RAPD) markers in 27 F1 full siblings of Pinctada fucata from a single pair of parents was studied using eight 10-mer primers screened from 21. Of the total 49 loci amplified, 47 loci are normal, accounting for 95.9%, and two loci are unexpectedly amplified in F1 but not in parents. There are 44 loci segregating in the F1 individuals, accounting for 93.6%. Chi square tests indicate that the segregation of 12 loci are deviated significantly from Mendelian fashion, while the segregation of 32 loci are in line with Mendelian fashion, among which 1 : 1 segregating loci account for 43.8% and 3 : 1 segregating loci 56.2%. Among the 32 Mendelian segregation loci, paternal specific, maternal specific and biparental loci account for 28.2%, 15.6%和56.2%, respectively, indicating that the number of male specific markers is slightly larger than that of female specific markers. The high percentage of 1 : 1 segregating loci revealed in this study indicates that RAPD markers are useful in construction of linkage map of P.fucata using F1 family materials based on double pseudo-testcross configuration.

  • [1]
    Williams J G, Kubelik A R, Livak K J, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J]. Nucleic Acids Res, 1990, 18(22): 6531-6535. doi: 10.1093/nar/18.22.6531
    [2]
    Welsh J, Michael M. Fingerprinting genomes using PCR with arbitrary primers[J]. Nucleic Acids Res, 1990, 18(24) : 7213-7218. doi: 10.1093/nar/18.24.7213
    [3]
    Klinbunga S, Ampayup P, Tassanakajon A, et al. Development of species-specific markers of the tropical oyster (Crassostrea belcheri) in Thailand[J]. Mar Biotechnol, 2000, 2(5): 476-484. doi: 10.1007/s101260000028
    [4]
    Tassanakajon A, Pongsomboon S, Jarayabhand P, et al. Genetic structure in wild populations of black tiger shrimp (Penaeus monodom) using randomly amplified polymorphic DNA analysis[J]. J Mar Biotechnol, 1998, 6(4): 249-254. doi: 10.2522/ptj.20100163
    [5]
    Yue G, Li Y, Chen F, et al. Comparison of three DNA marker systems for assessing genetic diversity in Asian arowana (Scleropages formosus)[J]. Electrophoresis, 2002, 23(7-8): 1025-1032. doi: 10.1002/1522-2683(200204)23:7/8<1025::AID-ELPS1025>3.0.CO;2-Y
    [6]
    Byrne M, Murreli J C, Allen B. An integrated genetic linkage map for eucalypts using RFLP, RAPD and isozyme markers[J]. Theor Appl Genet, 1995, 91(6-7) : 869-875. doi: 10.1007/BF00223894
    [7]
    Hashizume T, Shimamoto I, Harughima Y, et al. Construction of a linkage map for watermelon[Citrullus lanatus (Thunb)] using random amplified polymorphic DNA (RAPD)[J]. Euphytica, 1996, 90(3) : 265-273. doi: 10.1007/BF00027475
    [8]
    Kubisiak T L, Nelson C D, Nance W L, et al. RAPD linkage mapping in a longleaf pine×slash pine F1 family[J]. Theor Appl Genet, 1995, 90(7): 1199-1127. doi: 10.1007/BF00222931
    [9]
    尹佟明, 朱立煌, 黄敏仁, 等. 利用RAPD标记构建响叶杨和银白杨分子标记连锁图谱[J]. 植物学报, 1999, 41(9) : 956-961. doi: 10.3321/j.issn:1672-9072.1999.09.009
    [10]
    Hemmat M, Weeden N F, Manganaris A G, et al. Molecular marker linkage map for apple[J]. J Hered, 1994, 85(1): 4-11. doi: 10.1046/j.1420-9101.1994.7010097.x
    [11]
    甘四明, 施季森, 白嘉雨, 等. RAPD标记在桉属种间杂交一代的分离方式研究[J]. 林业科学研究, 2001, 14(2) : 125-130. doi: 10.3321/j.issn:1001-1498.2001.02.002
    [12]
    刘孟军, Shin Y U, Yae B W. RAPD标记在苹果属种间杂交一代的分离方式[J]. 园艺学报, 1998, 25(3) : 214-219.
    [13]
    申雪艳, 孔杰, 岳志芹, 等. RAPD标记在中国对虾Fenneropenaeus chinensis单对交配亲本及其子一代的分离方式[J]. 高技术通讯, 2004, 14 (9): 100-105. doi: 10.3321/j.issn:1002-0470.2004.09.022
    [14]
    Liu Z, Li P, Argue B J, et al. Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2 and backcross hybrids[J]. Anim Genet, 1998, 29(1): 58-62. doi: 10.1046/j.1365-2052.1998.00284.x
    [15]
    Appleyard S A, Mather P B. Investigation into the mode of inheritance of allozyme and random amplified polymorphic DNA markers in tilapia Oreochromis mossambicus (Peters)[J]. Aquac Res, 2000, 31(4): 435-445. doi: 10.1046/j.1365-2109.2000.00464.x
    [16]
    Elo K, Ivanoff S, Vuorinen J A, et al. Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and Atlantic salmon[J]. Aquac, 1997, 152(1): 55-65. doi: 10.1016/S0044-8486(96)01529-3
    [17]
    房经贵, 章镇, 马正强, 等. AFLP标记在两个芒果品种间杂交F1代的多态性及分离方式[J]. 中国农业科学, 2000, 33(3) : 19-24. doi: 10.3321/j.issn:0578-1752.2000.03.004
    [18]
    Liu Z, Nichols A, Li P, et al. Inheritance and usefulness of AFLP markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2, and backcross hybrids[J]. Mol Gen Genet, 1998, 258(3): 260-268. doi: 10.1046/j.1365-2052.1998.00284.x
    [19]
    Li Q, Park C, Kobayashi T, et al. Inheritance of microsatellite DNA markers in the Pacific abalone Haliotis discus hannai[J]. Mar Biotechnol, 2003, 5(4): 331-338. doi: 10.1007/s10126-002-0116-8
    [20]
    Spruell P, Pilgrim K L, Greene B A, et al. Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon[J]. J Hered, 1999, 90(2): 289-296.
    [21]
    王爱民, 邓凤姣, 张锡元, 等. 马氏珠母贝遗传多样性的RAPD分析[J]. 武汉大学学报, 2000, 46(4) : 467-470. doi: 10.3321/j.issn:1671-8836.2000.04.017
    [22]
    王爱民, 阎冰, 叶力, 等. 三个野生种群马氏珠母贝遗传多样性的RAPD分析[J]. 农业生物技术学报, 2003, 11(2) : 163-168.
    [23]
    苏天凤, 蔡云川, 张殿昌, 等. 合浦珠母贝3个养殖群体的RAPD分析[J]. 中国水产科学, 2002, 9(2) : 106-109. doi: 10.3321/j.issn:1005-8737.2002.02.003
    [24]
    Liu Z J, Cordes J F. DNA marker technologies and their applications in aquaculture genetics[J]. Aquac, 2004, 238(1): 1-37. doi: 10.1016/j.aquaculture.2004.05.027
    [25]
    Faure S, Noyer J L, Horry J P. A molecular marker-based linkage map of diploid bananas (Musa acuminata)[J]. Theor Appl Genet, 1993, 87(4) : 517-526. doi: 10.1007/BF00215098
    [26]
    甘四明, 施季森, 白嘉雨, 等. 尾叶桉和细叶桉无性系的RAPD指纹图谱构建[J]. 南京林业大学学报, 1999, 23(1) : 11-14. doi: 10.3969/j.issn.1000-2006.1999.01.003
    [27]
    Ayliffe M A, Lawrence G J, Ellis J G, et al. Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands[J]. Nucleic Acid Res, 1994, 22(9) : 1632-1636. doi: 10.1093/nar/22.9.1632
    [28]
    刘萍, 孔杰, 石拓, 等. 中国对虾黄渤海沿岸群亲本及子一代RAPD分析[J]. 海洋水产研究, 2000, 21(1) : 13-21. doi: 10.3969/j.issn.1000-7075.2000.01.003
    [29]
    Van Open M J H. Tracking dispersal rates: phylogeography of the Antarctic disjunct seaweed Acrosiphonia arcta[J]. J Phycol, 1994, 30(1): 67-80. doi: 10.1111/j.0022-3646.1994.00067.x
  • Related Articles

    [1]CAO Liu, MA Jun, LU Yan, WU Yatong, WANG Jun, ZHONG Lijing, SU Shanshan, HUANG Hai. Molecular characterization and SNPs association with growth-related traits of myosin heavy chains in hybrid grouper (Epinephelus fuscoguttatus ♀ × E. polyphekadion ♂)[J]. South China Fisheries Science. DOI: 10.12131/20240294
    [2]LI Bingbu, WANG Guixing, ZHANG Xiaoyan, LIU Yufeng, HE Zhongwei, CAO Wei, REN Jiangong, REN Yuqin, ZHANG Yitong, SAN Lize, WANG Yufen, HOU Jilun. Association analysis of SNPs polymorphisms in mstn gene of Paralichthys olivaceus with growth traits[J]. South China Fisheries Science, 2024, 20(2): 119-128. DOI: 10.12131/20230165
    [3]WANG Lun, WANG Chongyi, LIU Jianyong. Evaluation of genetic parameters for growth and comprehensive stress tolerance traits of Litopenaeus vannamei[J]. South China Fisheries Science, 2022, 18(4): 95-102. DOI: 10.12131/20210252
    [4]CHEN Jian, GUO Dan, ZHAI Ziqing, YU Dahui, BAI Lirong. Correlation analysis of morphological traits and body mass traits of Coelomactra antiquata[J]. South China Fisheries Science, 2021, 17(1): 45-51. DOI: 10.12131/20200180
    [5]LI Zhimin, QIAN Jiahui, LIU Jianyong, AI Jialin, TAN Keqin. Molecular cloning, expression of alpha-amylase gene of Haliotis diversicolor supertexta and correlation with growth traits[J]. South China Fisheries Science, 2017, 13(6): 14-21. DOI: 10.3969/j.issn.2095-0780.2017.06.002
    [6]GUO Huayang, LI Youning, ZHANG Nan, ZHU Kecheng, WANG Yu, ZHANG Dianchang. Study on development rules and growth curve fitting of early cultured winged pearl oyster (Pteria penguin)[J]. South China Fisheries Science, 2016, 12(5): 71-80. DOI: 10.3969/j.issn.2095-0780.2016.05.009
    [7]LIN Mingxue, YANG Jie, YUE Wucheng, KANG Wei, LEI Shuangyong, DU Jinxing, WANG Chenghui. Analysis of combining ability, heterosis and genetic correlation on growth traits of GIFT tilapia[J]. South China Fisheries Science, 2016, 12(2): 1-6. DOI: 10.3969/j.issn.2095-0780.2016.02.001
    [8]NIU Zhikai, LIU Baosuo, ZHANG Dongling, TAN Caigang, ZHANG Bo, CHEN Mingqiang, FAN Sigang, JIANG Song, HUANG Guiju, LI Youning, YU Dahui. Comparative analysis of growth traits and shell-closing strength among hybrid populations from three geographical groups of pearl oyster (Pinctada fucata)[J]. South China Fisheries Science, 2015, 11(1): 26-32. DOI: 10.3969/j.issn.2095-0780.2015.01.004
    [9]LIU Wei, SU Shengyan, DONG Zaijie, ZHANG Jianqiao, MA Liangxiao, LI Lingling, QV Jiangqi, YUAN Xinhua. Correlation analysis of microsatellite DNA markers with growth trait among 3 breeding populations of common carp[J]. South China Fisheries Science, 2012, 8(3): 17-24. DOI: 10.3969/j.issn.2095-0780.2012.03.003
    [10]TONG Xin, GONG Shiyuan, YU Dahui, DU Bo, HUANG Guiju, LI Lihao, GUO Yihui, LI Sedong. Variation of growth traits at different generations of Pacific white shrimp (Litopenaeus vannamei)[J]. South China Fisheries Science, 2007, 3(6): 30-33.

Catalog

    Article views (5324) PDF downloads (3585) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return