Volume 1 Issue 6
Dec.  2005
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YU Dahui, WANG Xiaoyu, GUO Yihui, HUANG Guiju, WANG Aimin. Segregation of RAPD markers in F1 generation of Pinctada fucata family[J]. South China Fisheries Science, 2005, 1(6): 1-7.
Citation: YU Dahui, WANG Xiaoyu, GUO Yihui, HUANG Guiju, WANG Aimin. Segregation of RAPD markers in F1 generation of Pinctada fucata family[J]. South China Fisheries Science, 2005, 1(6): 1-7.
shu

Segregation of RAPD markers in F1 generation of Pinctada fucata family

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Fisheries College, Huazhong Agricultural University, Wuhan 430070, China

  • 3.

    Ocean College, Hainan University, Haikou 57000, China

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  • Received Date: August 09, 2005
  • Revised Date: August 21, 2005
    Graphical Abstract
    • Abstract

  • The mode of segregation of random amplified polymorphic DNA (RAPD) markers in 27 F1 full siblings of Pinctada fucata from a single pair of parents was studied using eight 10-mer primers screened from 21. Of the total 49 loci amplified, 47 loci are normal, accounting for 95.9%, and two loci are unexpectedly amplified in F1 but not in parents. There are 44 loci segregating in the F1 individuals, accounting for 93.6%. Chi square tests indicate that the segregation of 12 loci are deviated significantly from Mendelian fashion, while the segregation of 32 loci are in line with Mendelian fashion, among which 1 : 1 segregating loci account for 43.8% and 3 : 1 segregating loci 56.2%. Among the 32 Mendelian segregation loci, paternal specific, maternal specific and biparental loci account for 28.2%, 15.6%和56.2%, respectively, indicating that the number of male specific markers is slightly larger than that of female specific markers. The high percentage of 1 : 1 segregating loci revealed in this study indicates that RAPD markers are useful in construction of linkage map of P.fucata using F1 family materials based on double pseudo-testcross configuration.

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    • References (29)
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