Volume 1 Issue 6
Dec.  2005
Turn off MathJax
Article Contents
Abstract
References
YU Dahui, WANG Xiaoyu, GUO Yihui, HUANG Guiju, WANG Aimin. Segregation of RAPD markers in F1 generation of Pinctada fucata family[J]. South China Fisheries Science, 2005, 1(6): 1-7.
Citation: YU Dahui, WANG Xiaoyu, GUO Yihui, HUANG Guiju, WANG Aimin. Segregation of RAPD markers in F1 generation of Pinctada fucata family[J]. South China Fisheries Science, 2005, 1(6): 1-7.
shu

Segregation of RAPD markers in F1 generation of Pinctada fucata family

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Fisheries College, Huazhong Agricultural University, Wuhan 430070, China

  • 3.

    Ocean College, Hainan University, Haikou 57000, China

More Information
  • Received Date: August 09, 2005
  • Revised Date: August 21, 2005
    Graphical Abstract
    • Abstract

  • The mode of segregation of random amplified polymorphic DNA (RAPD) markers in 27 F1 full siblings of Pinctada fucata from a single pair of parents was studied using eight 10-mer primers screened from 21. Of the total 49 loci amplified, 47 loci are normal, accounting for 95.9%, and two loci are unexpectedly amplified in F1 but not in parents. There are 44 loci segregating in the F1 individuals, accounting for 93.6%. Chi square tests indicate that the segregation of 12 loci are deviated significantly from Mendelian fashion, while the segregation of 32 loci are in line with Mendelian fashion, among which 1 : 1 segregating loci account for 43.8% and 3 : 1 segregating loci 56.2%. Among the 32 Mendelian segregation loci, paternal specific, maternal specific and biparental loci account for 28.2%, 15.6%和56.2%, respectively, indicating that the number of male specific markers is slightly larger than that of female specific markers. The high percentage of 1 : 1 segregating loci revealed in this study indicates that RAPD markers are useful in construction of linkage map of P.fucata using F1 family materials based on double pseudo-testcross configuration.

    • FullText(HTML)
    • References (29)
  • [1]
    Williams J G, Kubelik A R, Livak K J, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J]. Nucleic Acids Res, 1990, 18(22): 6531-6535. doi: 10.1093/nar/18.22.6531
    [2]
    Welsh J, Michael M. Fingerprinting genomes using PCR with arbitrary primers[J]. Nucleic Acids Res, 1990, 18(24) : 7213-7218. doi: 10.1093/nar/18.24.7213
    [3]
    Klinbunga S, Ampayup P, Tassanakajon A, et al. Development of species-specific markers of the tropical oyster (Crassostrea belcheri) in Thailand[J]. Mar Biotechnol, 2000, 2(5): 476-484. doi: 10.1007/s101260000028
    [4]
    Tassanakajon A, Pongsomboon S, Jarayabhand P, et al. Genetic structure in wild populations of black tiger shrimp (Penaeus monodom) using randomly amplified polymorphic DNA analysis[J]. J Mar Biotechnol, 1998, 6(4): 249-254. doi: 10.2522/ptj.20100163
    [5]
    Yue G, Li Y, Chen F, et al. Comparison of three DNA marker systems for assessing genetic diversity in Asian arowana (Scleropages formosus)[J]. Electrophoresis, 2002, 23(7-8): 1025-1032. doi: 10.1002/1522-2683(200204)23:7/8<1025::AID-ELPS1025>3.0.CO;2-Y
    [6]
    Byrne M, Murreli J C, Allen B. An integrated genetic linkage map for eucalypts using RFLP, RAPD and isozyme markers[J]. Theor Appl Genet, 1995, 91(6-7) : 869-875. doi: 10.1007/BF00223894
    [7]
    Hashizume T, Shimamoto I, Harughima Y, et al. Construction of a linkage map for watermelon[Citrullus lanatus (Thunb)] using random amplified polymorphic DNA (RAPD)[J]. Euphytica, 1996, 90(3) : 265-273. doi: 10.1007/BF00027475
    [8]
    Kubisiak T L, Nelson C D, Nance W L, et al. RAPD linkage mapping in a longleaf pine×slash pine F1 family[J]. Theor Appl Genet, 1995, 90(7): 1199-1127. doi: 10.1007/BF00222931
    [9]
    尹佟明, 朱立煌, 黄敏仁, 等. 利用RAPD标记构建响叶杨和银白杨分子标记连锁图谱[J]. 植物学报, 1999, 41(9) : 956-961. doi: 10.3321/j.issn:1672-9072.1999.09.009
    [10]
    Hemmat M, Weeden N F, Manganaris A G, et al. Molecular marker linkage map for apple[J]. J Hered, 1994, 85(1): 4-11. doi: 10.1046/j.1420-9101.1994.7010097.x
    [11]
    甘四明, 施季森, 白嘉雨, 等. RAPD标记在桉属种间杂交一代的分离方式研究[J]. 林业科学研究, 2001, 14(2) : 125-130. doi: 10.3321/j.issn:1001-1498.2001.02.002
    [12]
    刘孟军, Shin Y U, Yae B W. RAPD标记在苹果属种间杂交一代的分离方式[J]. 园艺学报, 1998, 25(3) : 214-219.
    [13]
    申雪艳, 孔杰, 岳志芹, 等. RAPD标记在中国对虾Fenneropenaeus chinensis单对交配亲本及其子一代的分离方式[J]. 高技术通讯, 2004, 14 (9): 100-105. doi: 10.3321/j.issn:1002-0470.2004.09.022
    [14]
    Liu Z, Li P, Argue B J, et al. Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2 and backcross hybrids[J]. Anim Genet, 1998, 29(1): 58-62. doi: 10.1046/j.1365-2052.1998.00284.x
    [15]
    Appleyard S A, Mather P B. Investigation into the mode of inheritance of allozyme and random amplified polymorphic DNA markers in tilapia Oreochromis mossambicus (Peters)[J]. Aquac Res, 2000, 31(4): 435-445. doi: 10.1046/j.1365-2109.2000.00464.x
    [16]
    Elo K, Ivanoff S, Vuorinen J A, et al. Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and Atlantic salmon[J]. Aquac, 1997, 152(1): 55-65. doi: 10.1016/S0044-8486(96)01529-3
    [17]
    房经贵, 章镇, 马正强, 等. AFLP标记在两个芒果品种间杂交F1代的多态性及分离方式[J]. 中国农业科学, 2000, 33(3) : 19-24. doi: 10.3321/j.issn:0578-1752.2000.03.004
    [18]
    Liu Z, Nichols A, Li P, et al. Inheritance and usefulness of AFLP markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2, and backcross hybrids[J]. Mol Gen Genet, 1998, 258(3): 260-268. doi: 10.1046/j.1365-2052.1998.00284.x
    [19]
    Li Q, Park C, Kobayashi T, et al. Inheritance of microsatellite DNA markers in the Pacific abalone Haliotis discus hannai[J]. Mar Biotechnol, 2003, 5(4): 331-338. doi: 10.1007/s10126-002-0116-8
    [20]
    Spruell P, Pilgrim K L, Greene B A, et al. Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon[J]. J Hered, 1999, 90(2): 289-296.
    [21]
    王爱民, 邓凤姣, 张锡元, 等. 马氏珠母贝遗传多样性的RAPD分析[J]. 武汉大学学报, 2000, 46(4) : 467-470. doi: 10.3321/j.issn:1671-8836.2000.04.017
    [22]
    王爱民, 阎冰, 叶力, 等. 三个野生种群马氏珠母贝遗传多样性的RAPD分析[J]. 农业生物技术学报, 2003, 11(2) : 163-168.
    [23]
    苏天凤, 蔡云川, 张殿昌, 等. 合浦珠母贝3个养殖群体的RAPD分析[J]. 中国水产科学, 2002, 9(2) : 106-109. doi: 10.3321/j.issn:1005-8737.2002.02.003
    [24]
    Liu Z J, Cordes J F. DNA marker technologies and their applications in aquaculture genetics[J]. Aquac, 2004, 238(1): 1-37. doi: 10.1016/j.aquaculture.2004.05.027
    [25]
    Faure S, Noyer J L, Horry J P. A molecular marker-based linkage map of diploid bananas (Musa acuminata)[J]. Theor Appl Genet, 1993, 87(4) : 517-526. doi: 10.1007/BF00215098
    [26]
    甘四明, 施季森, 白嘉雨, 等. 尾叶桉和细叶桉无性系的RAPD指纹图谱构建[J]. 南京林业大学学报, 1999, 23(1) : 11-14. doi: 10.3969/j.issn.1000-2006.1999.01.003
    [27]
    Ayliffe M A, Lawrence G J, Ellis J G, et al. Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands[J]. Nucleic Acid Res, 1994, 22(9) : 1632-1636. doi: 10.1093/nar/22.9.1632
    [28]
    刘萍, 孔杰, 石拓, 等. 中国对虾黄渤海沿岸群亲本及子一代RAPD分析[J]. 海洋水产研究, 2000, 21(1) : 13-21. doi: 10.3969/j.issn.1000-7075.2000.01.003
    [29]
    Van Open M J H. Tracking dispersal rates: phylogeography of the Antarctic disjunct seaweed Acrosiphonia arcta[J]. J Phycol, 1994, 30(1): 67-80. doi: 10.1111/j.0022-3646.1994.00067.x
    • Related Articles

Catalog

    Get Citation
    PDF
    XML
    Article views (5313) PDF downloads (3574) Cited by()
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return