Segregation of RAPD markers in F1 generation of Pinctada fucata family

The mode of segregation of random amplified polymorphic DNA (RAPD) markers in 27 F1 full siblings of Pinctada fucata from a single pair of parents was studied using eight 10-mer primers screened from 21. Of the total 49 loci amplified, 47 loci are normal, accounting for 95.9%, and two loci are unexpectedly amplified in F1 but not in parents. There are 44 loci segregating in the F1 individuals, accounting for 93.6%. Chi square tests indicate that the segregation of 12 loci are deviated significantly from Mendelian fashion, while the segregation of 32 loci are in line with Mendelian fashion, among which 1:1 segregating loci account for 43.8% and 3:1 segregating loci 56.2%. Among the 32 Mendelian segregation loci, paternal specific, maternal specific and biparental loci account for 28.2%，15.6%和56.2%, respectively, indicating that the number of male specific markers is slightly larger than that of female specific markers. The high percentage of 1:1 segregating loci revealed in this study indicates that RAPD markers are useful in construction of linkage map of P.fucata using F1 family materials based on double pseudo-testcross configuration.