Sequence analysis of the internal transcribed spacer 1(ITS-1） of ribosomal DNA gene of Lutjanus fulviflamma

The PCR technique was used to amplify rDNA-ITS-1 of Lutjanus fulviflamma, then the purified PCR productions were cloned into T-vector and sequenced by M13+/-primers. As a result, 566 bp nucleotide sequences of rDNA-ITS1 were obtained. The average contents of A, T, G and C were 14.1%, 16.1%, 30.2% and 39.6%, respectively, the contents of GC (69.8%） were obviously higher than those of AT. Using these primers, ITS-1 region can be amplified in other four Lutjanus species, but the lengths of ITS-1 were largely different between different species.