Volume 2 Issue 5
Oct.  2006
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XU Tianjun, LIU Chuwu, LIU Li, WU Yong. Sequence analysis of the internal transcribed spacer 1(ITS-1) of ribosomal DNA gene of Lutjanus fulviflamma[J]. South China Fisheries Science, 2006, 2(5): 61-64.
Citation: XU Tianjun, LIU Chuwu, LIU Li, WU Yong. Sequence analysis of the internal transcribed spacer 1(ITS-1) of ribosomal DNA gene of Lutjanus fulviflamma[J]. South China Fisheries Science, 2006, 2(5): 61-64.
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Sequence analysis of the internal transcribed spacer 1(ITS-1) of ribosomal DNA gene of Lutjanus fulviflamma

  • Marine Biological Research Institute, Guangdong Ocean University, Zhanjiang 524025, China

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  • Received Date: June 02, 2006
  • Revised Date: July 16, 2006
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  • The PCR technique was used to amplify rDNA-ITS-1 of Lutjanus fulviflamma, then the purified PCR productions were cloned into T-vector and sequenced by M13+/-primers.As a result, 566 bp nucleotide sequences of rDNA-ITS1 were obtained. The average contents of A, T, G and C were 14.1%, 16.1%, 30.2% and 39.6%, respectively, the contents of GC (69.8%) were obviously higher than those of AT. Using these primers, ITS-1 region can be amplified in other four Lutjanus species, but the lengths of ITS-1 were largely different between different species.

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