Volume 3 Issue 1
Feb.  2007
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FAN Wujiang, WANG Xiaoqing, YANG Pinhong, XIE Chunhua. Comparison of genomic DNA extraction of Aristichthys nobilis[J]. South China Fisheries Science, 2007, 3(1): 44-47.
Citation: FAN Wujiang, WANG Xiaoqing, YANG Pinhong, XIE Chunhua. Comparison of genomic DNA extraction of Aristichthys nobilis[J]. South China Fisheries Science, 2007, 3(1): 44-47.
shu

Comparison of genomic DNA extraction of Aristichthys nobilis

  • 1.

    The Animal Science and Technology Institute of Hunan Agricultural University, Changsha 410008, China

  • 2.

    Aquatic Engineering Technology Research Center of Hunan Province, Changde 415000, China

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  • Received Date: September 19, 2006
  • Revised Date: October 19, 2006
    Graphical Abstract
    • Abstract

  • Two methods (Genomic DNA extraction kit and Phe/Chl)were used to extract genomic DNA from fresh muscles, livers and caudal fins, and alcohol-preserved ones of bighead carps. The results showed that both of the methods can extract high-quality DNA from the three organizations under different conditions. The genomic DNA can be used for the further molecular biology research. The DNA samples of caudal fins preserved in alcohol are the best quality, followed by muscle, liver the worst. The results showed the DNA extraction kit could obtain high purity genomic DNA with low density, and high concentration, good quality and relatively low cost by Phe/Chl method. The phenol extraction of genomic DNA from alcohol-preserved caudal fin is an effective method of promoting and using in molecular studies on fish.

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    • References (10)
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