Synergistic effect of Vitamin E and Schizochytrium sp. on spermatophore regeneration in Penaeus monodon

To investigate the effects of Vitamin E and Schizochytrium sp. on the spermatophore regeneration in Penaeus monodon, we designed four different contents of Vitamin E and Schizochytrium sp. in the basal diet (control group; V_E 200; 1% Schizochytrium sp.; V_E 200+1% Schizochytrium sp.) to feed mature male P.monodon which had been extruded spermatophore for 40 d. The results show that the percentage of viable spermatozoa added in 200 mg·kg^–1 Vitamin E diet group was significantly higher than that in the control group (P<0.05). The sperm quantity of single added in 1% Schizochytrium sp. diet group was not significantly different with control group (P>0.05). The total number of spermatozoa fed with 200 mg·kg^–1 of Vitamin E and 1% Schizochytrium sp. was significantly higher than that of the control group (P<0.05). According to the spermatophore quality, sperm quantity and living sperm percentage, adding 200 mg·kg^–1 of Vitamin E and 1% Schizochytrium sp. in feed can promote the spermatophore regeneration in the male P.monodon and improve the sperm quantity effectively.