Feasibility of flow cytometry experiments based on fin

Flow cytometry, which is widely used in fish breeding, can detect cell cycle and DNA content of living organisms rapidly. However, the current studies mainly focus on experiments based on fish blood and germ cells, which have limitation in periodicity and operation. We used different tissues of Ctenopharyngodon idellus by flow cytometry to obtain a desired and stable histogram showing DNA content of the fin tissues from four different fishes. All fin tissues exhibited cell cycle phenomenon, and low ratio appeared at time phase of G₀/G₁. Besides, Danio rerio was used as internal calibration to detect the DNA content (C value) of Megalobrama amblycephala (1.25±0.07) pg, and the result has no significant difference with the results of other studies. Therefore, it is concluded that use of fin as alternative for blood and germ cells for flow cytometry research is scientific and feasible.