| Citation: |
ZHANG Wenling, MU Xidong, HU Yinchang, SONG Hongmei. Cloning and expression analysis of cytochrome P450 gene CYP3192A1 from Pomacea canaliculata[J]. South China Fisheries Science, 2017, 13(1): 66-75. DOI: 10.3969/j.issn.2095-0780.2017.01.009
|
The study investigates the structure and expression features of Pomacea canaliculata cytochrome P450, and explores the correlation of metaldehyde treatment and cytochrome P450 expression level. A full-length cDNA of CYP3192A1 from P.canaliculata with total length of 2 523 bp was obtained by RACE, including an open reading frame (ORF) encoding 517 amino acids, which had a heme-binding domain F**G*** C*G, helix K(E**R), helix I(AG*ET), helix C(W***R) and trans-membrane helix(12~34 amino acids).Blast and phylogenetic analyses suggest P.canaliculata CYP3192A1 is a member of a novel CYP450 family, with high similarity with CYP3A subfamily. The RT-qPCR suggests that CYP3192A1 has gender-specific and tissue specific expression(P < 0.05). Expression in female P.canaliculata was higher than in males; expression amounts in intestinal and gill were the highest, followed by those in liver and stomach, and were the lowest in heart and pleopod. The influence of metaldehyde to CYP3192A1 expression shows a trend of induction at low concentration, shortened and advanced induction period at high concentration. It is demonstrated that there is close correlation between CYP3192A1 expression and metaldehyde treatment, and the induced expression of CYP3192A1 might play an important role in metaldehyde metalbolism.
| [1] |
LIU X N, LIANG P, GAO X W, et al. Induction of cytochrome P450 activity by plant allelochemicals in the cotton bollworm, Helicoverpa armigera[J].Pestic Biochem Physiol, 2006, 84(2):127-134. doi: 10.1016/j.pestbp.2005.06.002
|
| [2] |
NELSON D R, KAMATAKI T, WAXMAN D J. The P450 super family:update on new sequences, gene mapping, accession numbers, early trivial names of enzymes nomenclature[J].DNA Cell Biol, 1993, 12(1):1-51. doi: 10.1089/dna.1993.12.1
|
| [3] |
郭艳琼. 飞蝗细胞色素P450的分析特性及功能研究[D]. 太原: 山西大学, 2012: 4-6. http://cdmd.cnki.com.cn/Article/CDMD-10108-1012484957.htm
|
| [4] |
郭亭亭, 姜辉, 高希武.昆虫细胞色素P450基因的多样性、进化及表达调控[J].昆虫学报, 2009, 52(2):301-311. doi: 10.7679/j.issn.2095-1353.2009.055
|
| [5] |
NELSON D R. The Cytochrome P450 Homepage[J].Human Genomics, 2009, 4:59-65. doi: 10.1186/1479-7364-4-1-59.pdf
|
| [6] |
TIAN S M, PAN L Q, ZHANG H. Identification of a CYP3A-like gene and CYPs mRNA expression modulation following exposure to benzo[a]pyrene in the bivalve mollusk Chlamys farreri[J].Mar Environ Res, 2014, 94:7-15. doi: 10.1016/j.marenvres.2013.11.001
|
| [7] |
PAN L Q, LIU N, XU C Q, et al. Identification of a novel P450 gene belonging to the CYP4 family in the clam Ruditapes philippinarum, and analysis of basal-and benzo(a)pyrene-induced mRNA expression levels in selected tissues [J].Environ Toxicol Pharm, 2011, 32(3):390-398. doi: 10.1016/j.etap.2011.08.005
|
| [8] |
CHEUNG C C C, ZHENG G J, LAM P K S, et al. Relationships between tissue concentrations of chlorinated hydrocarbons (polychlorinated biphenyls and chlorinated pesticides) and antioxidative responses of marine mussels, Perna viridis[J].Mar Pollut Bull, 2002, 45:181-191. doi: 10.1016/S0025-326X(01)00301-0
|
| [9] |
TOLEDO-SILVA G, SIEBERT M N, MEDEIROS L D, et al. Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas[J].Mar Environ Res, 2008, 66:15-18. doi: 10.1016/j.marenvres.2008.02.010
|
| [10] |
杨叶欣, 胡隐昌, 李小慧, 等.福寿螺在中国的入侵历史、扩散规律和危害的调查分析[J].中国农学通报, 2010, 26(5):245-250. https://www.wenkuxiazai.com/doc/2211e8ebf90f76c661371ad9...
|
| [11] |
叶芳伶, 赖佩瑄, 黄大骏.台湾各地区福寿螺(Pomacea canaliculata)抗药性初步探讨[J].贝类学报, 2010, 34:49-61.
|
| [12] |
刘晓漫. 福寿螺不同地理种群抗药性及其生理生化差异研究[D]. 南宁: 广西大学, 2011: 23-25. http://cdmd.cnki.com.cn/Article/CDMD-10593-1012316196.htm
|
| [13] |
符贵红, 杨先乐, 喻文娟, 等.草鱼肾细胞中细胞色素P450 3A基因诱导表达及其酶活性分析[J].中国水产科学, 2011, 18(4):720-727. http://www.doc88.com/p-5854540580397.html
|
| [14] |
邱星辉.细胞色素P450介导的昆虫抗药性的分子机制[J].昆虫学报, 2014, 57(4):477-482. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper...
|
| [15] |
LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Methods, 2001, 25:402-408. doi: 10.1006/meth.2001.1262
|
| [16] |
杨帆. 赤拟谷盗细胞色素P450 CYP345D3 cDNA基因克隆与序列分析[D]. 重庆: 西南大学, 2008: 3-6. http://cdmd.cnki.com.cn/article/cdmd-10635-2008092690.htm
|
| [17] |
卢业竑, WEST S B, 夏世钧.哺乳动物微粒体细胞色素P450的多样性[J].国外医学分子生物学分册, 1982, 4(2):78-81. http://journals.im.ac.cn/actamicrocn/ch/reader/create_pdf.aspx?...
|
| [18] |
罗浑金, 傅童生, 匡光伟.细胞色素P450的多样性与介导抗性的研究[J].陕西农业科学, 2006(1):24-26. http://www.cqvip.com/QK/96295X/200601/20964616.html
|
| [19] |
胡之璧, 贺丽虹, 赵淑娟.细胞色素P450的研究进展[J].江苏中医药, 2006, 10 (26):81-84. http://journal.9med.net/html/qikan/dxxb/jlyyxyxb/20059263/zs/...
|
| [20] |
艾均文, 龚昕, 薛红, 等.不同鳞翅目昆虫细胞色素P450基因(CYPs)的比较基因组学分析[J].农业生物技术学报, 2015, 23(2):244-252. http://kns.cnki.net/KCMS/detail/detail.aspx?filename=nysb201502012&dbname=CJFD&dbcode=CJFQ
|
| [21] |
崔颖璐. 细胞色素P450酶底物选择性及门控机制的结构特性研究[D]. 长春: 吉林大学, 2015: 7. http://cdmd.cnki.com.cn/Article/CDMD-10183-1015597214.htm
|
| [22] |
YAO M, DAI M H, LIU Z Y, et al. mRNA expression profiles of P450 3A enzymes in the liver and small intestine of the domestic pig[J].Res Vet Sci, 2012, 93(1):360-365. doi: 10.1016/j.rvsc.2011.06.013
|
| [23] |
GIBSON G G, PLANT N J, SWALES K E, et al. Receptor-dependent transcriptional activation of cytochrome P4503A genes:induction mechanisms, species differences and interindividual variation in man[J].Xenobiotica, 2002, 32:165-206. doi: 10.1080/00498250110102674
|
| [24] |
马军国, 周春娥, 李效宇.咪唑类离子液体处理对草鱼CYP3A和PXR基因表达的影响[J].水生态学杂志, 2013, 34 (6):70-75. http://d.wanfangdata.com.cn/Periodical_sstxzz201306012.aspx
|
| [25] |
SCOTT J G, LIU N, WEN Z. Insect cytochrome P450:diversity, insecticide resistance and tolerance to plant toxins[J].Comp Biochem & Physiol C, 1998, 121:147-155. https://www.researchgate.net/publication/251596432_Insect...
|
| [26] |
TREVISAN R, MELLO F D, DELAPEDRA G, et al. Gills as glutathione-dependent metabolic barrier in Pacific oysters Crassostrea gigas:absorption, metabolism and excretion of a model electrophile[J].Aquat Toxicol, 2016, 173:105-119. doi: 10.1016/j.aquatox.2016.01.008
|
| [27] |
RAVERA O. Monitoring of the aquatic environment by species accumulator of pollutants:a review[J]. J Limnol, 2001, 60 (1):63-78. https://www.researchgate.net/publication/228470944_Monitoring_of...
|
| [28] |
杨树, 李燕.细胞色素P450的性别依赖性表达及生长激素对其调控和机制的研究进展[J].中国药理学与毒理学杂志, 2013, 27(5):893 -897. doi: 10.3867/j.issn.1000-3002.2013...
|
| [29] |
ZHAO Y, LIU X Q, QIAN Z Y, et al.Sex-dependent metabolism of drugs [J].Prog Pharm Sci, 2001, 25 (5):289-293. http://en.cnki.com.cn/Article_en/CJFDTOTAL-YXJZ200105007.htm
|
| [30] |
朱磊, 胡晓, 房文红, 等.异育银鲫P450家族CYP3A136基因的克隆与表达[J].水产学报, 2011, 35(10):1450-1457. http://www.oalib.com/paper/1690242
|
| [31] |
张晓璐, 乐江.细胞色素P450的工具药选择及种属差异的研究进展[J].中国药理学与毒理学杂志, 2012, 26(10):697-701. http://www.wenkuxiazai.com/doc/82724b64bb4cf7ec4afed0e7.html
|
| [32] |
韩文素, 王丽红, 孙婳婳, 等.杀虫剂对昆虫的亚致死效应的研究进展[J].中国植保导刊, 2011, 11:15-20. http://www.cqvip.com/QK/97996A/201111/40040594.html
|
| [33] |
傅先源, 王洪全.温度对福寿螺生长发育的影响[J].水产学报, 1999, 23(1):20-26. http://www.cqvip.com/QK/90215X/200803/27731377.html
|
| [34] |
邓婧婷, 庄笑梅, 李桦.小肠首过代谢的研究进展[J].国际药学研究杂志, 2010, 37(4):269-276. https://www.wenkuxiazai.com/doc/cc16e204cc175527072208ef...
|
| [35] |
黄伟, 胡晓.细胞色素P450 3A4代谢机制有关方面的研究进展[J].中国临床药理学与治疗学, 2015, 20(2):223-229. http://d.wanfangdata.com.cn/Periodical/zglcylxyzlx201502020
|
| 1. |
凌慧，赵旺，王瑞旋，黄晟，刘晓靖，吴开畅，王江勇. 方斑东风螺养殖环境中细菌数量变动与环境因子的相关性和主成分分析. 生态科学. 2019(02): 130-137 .
| |
| 2. |
孙永婵，王瑞旋，赵曼曼，曹超，鲁义善，姚托，王江勇. 鲍消化道及其养殖水体异养菌的耐药性研究. 南方水产科学. 2017(03): 58-65 .
本站查看
| |
| 3. |
唐姝，熊金波，王凯，胡常巨，郑嘉来，张德民. 象山港海水网箱养殖区弧菌群落动态变化. 生物学杂志. 2017(04): 76-81 .
|
Supported by: Beijing Renhe Information Technology Co., Ltd.
粤公网安备 44010502001741号
DownLoad: