Volume 12 Issue 3
Jun.  2016
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WANG Zhengbin, LIU Yongtao, AI Xiaohui, YANG Tao, LIU Tianqiang. Microbiological inhibition method for determination of colistin residues in muscles of aquatic products[J]. South China Fisheries Science, 2016, 12(3): 98-105. DOI: 10.3969/j.issn.2095-0780.2016.03.013
Citation: WANG Zhengbin, LIU Yongtao, AI Xiaohui, YANG Tao, LIU Tianqiang. Microbiological inhibition method for determination of colistin residues in muscles of aquatic products[J]. South China Fisheries Science, 2016, 12(3): 98-105. DOI: 10.3969/j.issn.2095-0780.2016.03.013
shu

Microbiological inhibition method for determination of colistin residues in muscles of aquatic products

  • 1.

    Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China

  • 2.

    Tianmen Aquatic Product Technology Promotion Department, Tianman 431700, China

  • 3.

    Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China

  • 4.

    Tongwei Co., Ltd, Chengdu 610041, China

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  • Received Date: June 17, 2015
  • Revised Date: October 08, 2015
    Graphical Abstract
    • Abstract

  • Bordetella bronchiseptica were extracted with methanol-0.1%formic acid water (volume ratio 3:7) under ultrasonication, purified by solid phase extraction column, and blowed with nitrogen gas. Then we used B.bronchiseptica to detect the residues after the concentration of samples. Results show that colistin in the muscle tissue of aquatic products ranged from 0.4 μg·mL-1 to 2.4 μg·mL-1, with good linearity between drug concentration logarithm and the diameter of bacteriostatic (R2> 0.990 0). Colistin in muscle was fortified at 0.20 μg·g-1, 0.40 μg·g-1 and 0.60 μg·g-1, and the average recovery was 70.59%~70.59%, while the coefficient of variation was 3.22%~14.01%. The limits of determination were 100 μg·kg-1 in grass carp and prawn muscles, 75 μg·kg-1 in turtle and eel muscles, and 125 μg·kg-1 in crab muscle. The cylinder plate method for determination of colistin residues is good. All minimum detection of drugs meet the national standard of residue limits issued by Ministry of Agriculture of China.

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    • References (31)
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