Cloning and expression pattern of Siglec-4b like in Oreochromis niloticus

DU Juanjuan; DONG Junjian; YE Xing; SUN Chengfei; TIAN Yuanyuan; LU Maixin

doi:10.3969/j.issn.2095-0780.2016.01.010

We cloned the sialic acid-binding immunoglobulin superfamily lectin Siglec-4b like gene from neutrophilic granulocyte of Oreochromis niloticus, and then analyzed its sequence by bioinformatics approach and detected its mRNA expression levels during embryonic development and in tissues before and after artificial infection of Streptococcus agalactiae (GBS) by qPCR. The analysis shows that Siglec-4b like had specific domains and conservative amino acid residues similar to mammalian Siglec-4. During the embryonic development, a certain amount of Siglec-4b like expressed in eggs, and the expression increased after fertilization. As embryo developed, the expression continued to rise and reached the maximum amount at 7 hpf and then decreased gradually. Siglec-4b like mRNA was ubiquitously expressed in all the tested tissues of tilapia, and the expression levels were relatively high in gill, muscle, skin, head kidneys and intestines but very low in the other tested tissues. After being infected with GBS, the expression of Siglec-4b like in gill, head kidneys, intestines, muscle and skin decreased. The results provide references for further research on immune escape mechanism of GBS and regulation effect of Siglec-4b like on tilapia immune system.

[1]	卢迈新, 黄樟翰. 罗非鱼遗传育种研究[J]. 上海水产大学学报, 2005, 14(2): 186-191. doi: 10.3969/j.issn.1004-7271.2005.02.016
[2]	卢迈新. 罗非鱼链球菌病研究进展[J]. 南方水产, 2010, 6(1): 75-79. doi: 10.3969/j.issn.1673-2227.2010.01.013
[3]	卢迈新, 黎炯, 叶星, 等. 广东与海南养殖罗非鱼无乳链球菌的分离、鉴定与特性分析[J]. 微生物学通报, 2010, 37(5): 766-774. https://wswxtb.ijournals.cn/wswxtbcn/article/abstract/tb10050766
[4]	SLOTVED H C, KONG F R, LAMBERTSEN L A, et al. Serotype Ⅸ, a proposed new Streptococcus agalactiae serotype[J]. J Clin Microbiol, 2007, 45(9): 2929-2936. doi: 10.1128/JCM.00117-07
[5]	YE X, LI J, LU M X, et al. Identification and molecular typing of Streptococcus agalactiae isolated from pond-cultured tilapia in China[J]. Fish Sci, 2011, 77(4): 623-632. doi: 10.1007/s12562-011-0365-4
[6]	MARQUES M B, KASPER D L, PANGBURN M K, et al. Prevention of C3 deposition by capsular polysaccharide is a virulence mechanism of type Ⅲ group B Streptococci[J]. Infect Immun, 1992, 60(10): 3986-3993. doi: 10.1128/iai.60.10.3986-3993.1992
[7]	OETKE C, VINSON M C, JONES C, et al. Sialoadhesin deficient mice exhibit subtle changes in B- and T-cell populations and reduced immunoglobulin M levels[J]. Mol Cell Biol, 2006, 26(4): 1549-1557. doi: 10.1128/MCB.26.4.1549-1557.2006
[8]	章晓联. 糖基化与免疫[J]. 生命科学, 2011, 23(7): 685-694. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg1zbWt4MjAxMTA3MDA4GghiOHl6Nzk1dA%3D%3D
[9]	LEHMANN F, GATHJE H, KELM S, et al. Evolution of sialic acid-binding proteins: molecular cloning and expression of fish siglec-4[J]. Glycobiology, 2004, 14(11): 959-968. doi: 10.1093/glycob/cwh120
[10]	ENDO M, ARINLERTAREE C, RUANGPAN L, et al. A new method for collecting neutrophils using swim bladder[J]. Fish Sci, 1997, 63(4): 644-645. doi: 10.2331/fishsci.63.644
[11]	曹建萌, 卢迈新, 叶星, 等. 尼罗罗非鱼整胚原位杂交技术的建立和初步应用[J]. 水产学报, 2014, 38(11): 1847-1854. doi: 10.3724/SP.J.1231.2014.49346
[12]	ZHANG R, ZHANG L L, YE X, et al. Transcriptome profiling and digital gene expression analysis of Nile tilapia (Oreochromis niloticus) infected by Streptococcus agalactiae[J]. Mol Biol Rep, 2013, 40(10): 5657-5668. doi: 10.1007/s11033-013-2667-3
[13]	DONG J J, WU F, YE X, et al. β-Defensin in Nile tilapia (Oreochromis niloticus): sequence, tissue expression, and anti-bacterial activity of synthetic peptides[J]. Gene, 2015, 566(1): 23-31. doi: 10.1016/j.gene.2015.04.025
[14]	PILLAI S, NETRAVALI I A, CARIAPPA A, et al. Siglecs and immune regulation[J]. Annu Rev Immunol, 2012, 30: 357-392. doi: 10.1146/annurev-immunol-020711-075018
[15]	MAY A P, ROBINSON R C, VINSON M, et al. Crystal structure of the N-terminal domain of sialoadhesin in complex with 3' sialyllactose at 1.85: a resolution[J]. Mol Cell, 1998, 1(5): 719-728. doi: 10.1016/S1097-2765(00)80071-4
[16]	ANGATA T, VARKI A. Cloning, characterization, and phylogenetic analysis of Siglec-9, a new member of the CD33-related group of Siglecs. Evidence for co-evolution with sialic acid synthesis pathways[J]. J Biol Chem, 2000, 275(29): 22127-22135. doi: 10.1074/jbc.M002775200
[17]	BORGES L, HSU M L, FANGER N, et al. A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules[J]. J Immunol, 1997, 159(11): 5192-5196. doi: 10.4049/jimmunol.159.11.5192
[18]	Le DREAN E, VELY F, OLCESE L, et al. Inhibition of antigen-induced T cell response and antibody-induced NK cell cytotoxicity by NKG2A: association of NKG2A with SHP-1 and SHP-2 protein-tyrosine phosphatases[J]. J Immunol, 1998, 28(3): 264-276. doi: 10.1002/(SICI)1521-4141(199801)28:01<264::AID-IMMU264>3.0.CO;2-O
[19]	MURAILLE E, BRUHNS P, PESESSE X, et al. The SH2 domain containing inositol 5-phosphatase SHIP2 associates to the immunoreceptor tyrosine-based inhibition motif of Fc gammaRⅡB in B cells under negative signaling[J]. Immunol Lett, 2000, 72(1): 7-15. doi: 10.1016/S0165-2478(00)00162-0
[20]	INGRAM G A. Substances involved in the natural resistance of fish to infection: a review[J]. J Fish Biol, 1980, 16(1): 23-60. doi: 10.1111/j.1095-8649.1980.tb03685.x
[21]	TATNER M F, MANNING M J. The ontogenetic development of the reticulo-endothelial system in the rainbow trout, Salmo gairdneri Richardson[J]. J Fish Dis, 1985, 8(2): 189-195. doi: 10.1111/j.1365-2761.1985.tb01214.x
[22]	KANE D A, KIMMEL C B. The zebrafish midblastula transition[J]. Development, 1993, 119(2): 447-456. doi: 10.1242/dev.119.2.447
[23]	FILBIN M T. Myelin-associated inhibitors of axonal regeneration in the adult mammalian CNS[J]. Nat Rev Neurosci, 2003, 4(9): 703-713. doi: 10.1038/nrn1195
[24]	PILLAI S, NETRAVALI I A, CARIAPPA A, et al. Siglecs and immune regulation[J]. Annu Rev Immunol, 2012, 30(3): 357-392. doi: 10.1146/annurev-immunol-020711-075018
[25]	SECOMBES C J. Cellular defences of fish: an update[M] //PIKE A W, LEWIS J W. Parasitic diseases of fish samara. Britain: Dyfed Publishing Limited, 1994: 209-224.
[26]	BABA T, IMAMURA J, IZAWA K, et al. Cell-mediated protection in carp against Aeromonas hydrophila[J]. J Fish Dis, 1998, 11(2): 171-179. doi: 10.1111/j.1365-2761.1988.tb00536.x
[27]	DULAC C, TROPAK M B, CAMERON CURRY P, et al. Molecular characterization of the Schwann cell myelin protein, SMP: structural similarities within the immunoglobulin superfamily[J]. Neuron, 1992, 8(2): 323-334. doi: 10.1016/0896-6273(92)90298-R
[28]	MARTINI R, SCHACHNER M. Immunoelectron microscopic localization of neural cell adhesion molecules (Ll, N-CAM, and MAG) and their shared carbohydrate epitope and myelin basic protein in developing sciatic nerve[J]. J Cell Biol, 1986, 103: 2439-2448. doi: 10.1083/jcb.103.6.2439
[29]	WALTER R B, RADEN B W, ZENG R, et al. ITIM-dependent endocytosis of CD33-related Siglecs: role of intracellular domain, tyrosine phosphorylation, and the tyrosine phosphatases, Shp1 and Shp2[J]. J Leukocyte Biol, 2008, 83(1): 200-211. doi: 10.1189/jlb.0607388
[30]	CARLIN A F, UCHIYAMA S, CHANG Y C, et al. Molecular mimicry of host sialylated glycans allows a bacterial pathogen to engage neutrophil Siglec-9 and dampen the innate immune response[J]. Blood, 2009, 113(14): 3333-3336. doi: 10.1182/blood-2008-11-187302
[31]	CHANG Y C, OLSON J, BEASLEY F C, et al. Group B Streptococcus engages an inhibitory siglec through sialic acid mimicry to blunt innate immune and inflammatory responses in vivo[J]. PLoS Pathog, 2014, 10(1): e1003846. doi: 10.1371/journal.ppat.1003846
[32]	黄锦炉. 罗非鱼无乳链球菌病病原学、病理学及cpsE基因的原核表达研究[D]. 雅安: 四川农业大学, 2012: 97-98. https://cdmd.cnki.com.cn/Article/CDMD-10626-1013171578.htm

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Cloning and expression pattern of Siglec-4b like in Oreochromis niloticus

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Cloning and expression pattern of Siglec-4b like in Oreochromis niloticus

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