Volume 11 Issue 4
Aug.  2015
Turn off MathJax
Article Contents
Abstract
References
Related Articles
XIAO Wei, LI Dayu, XU Yang, ZOU Zhiying, ZHU Jinglin, HAN Jue, YANG Hong. Effects of chronic external ammonia stress on growth, immunity and metabolism of juvenile GIFT tilapia (Oreochromis niloticus)[J]. South China Fisheries Science, 2015, 11(4): 81-87. DOI: 10.3969/j.issn.2095-0780.2015.04.012
Citation: XIAO Wei, LI Dayu, XU Yang, ZOU Zhiying, ZHU Jinglin, HAN Jue, YANG Hong. Effects of chronic external ammonia stress on growth, immunity and metabolism of juvenile GIFT tilapia (Oreochromis niloticus)[J]. South China Fisheries Science, 2015, 11(4): 81-87. DOI: 10.3969/j.issn.2095-0780.2015.04.012
shu

Effects of chronic external ammonia stress on growth, immunity and metabolism of juvenile GIFT tilapia (Oreochromis niloticus)

  • 1.

    Key Lab. of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Wuxi 214081, China

  • 2.

    Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

More Information
  • Received Date: October 20, 2014
  • Revised Date: December 15, 2014
    Graphical Abstract
    • Abstract

  • To investigate the effects of chronic external ammonia stress on growth, immunity and metabolism of juvenile GIFT tilapia (Oreochromis niloticus), we designed five concentrations of ammonia, including 0 mg · L-1(A0), 1.63 mg · L-1(A1), 3.25 mg · L-1(A2), 6.51 mg · L-1(A3) and 13.01 mg · L-1(A4). The results show that after 30-day feeding, the juveniles′ weight gain rate (WGR) and specific growth rate (SGR) decreased significantly with increasing concentration of ammonia. For immunity, the number of white blood cells (WBC) increased but that of hemoglobin (HGB) decreased with increasing concentration of ammonia. Serum albumin(ALB) concentration and superoxide dismutase(SOD) activities of A3 and A4 were significantly less than A0, while alkaline phosphatase (AKP) concentration of A4 was significantly less than A0. For metabolization, serum triglycerides(TG) concentration of A3 and A4 were significantly less than A0, while total cholesterol (TC) concentration of A1~A4 were significantly less than A0. Serum alanine transaminase(ALT) activities of A3 and A4 were significantly less than A1 and A2. Meanwhile, only aspartate transaminase (AST) activities of A4 was less than A0. It is revealed that the growth, nonspecific immunity and metabolism of juvenile GIFT tilapia would be inhibited under chronic ammonia stress; change of ammonia concentration in tilapia aquaculture should be paid more attention to.

    • FullText(HTML)
    • References (31)
  • [1]
    Worldfish Center. GIFT technology manual: an aid to tilapia selective breeding[R]. Penang, Malaysia: Worldfish Center, 2004. https://xueshu.baidu.com/usercenter/paper/show?paperid=2cd2fbbf9f7c2fdd70cb57088a049a48&site=xueshu_se
    [2]
    刘华丽, 曹秀云, 宋春雷, 等. 水产养殖池塘沉积物有机质富集的环境效应与修复策略[J]. 水生态学杂志, 2011, 32(6): 130-134. doi: 10.3969/j.issn.1003-1278.2011.06.025
    [3]
    RANDALL D J, TSUI T K N. Ammonia toxicity in fish[J]. Mar Poll Bull, 2002, 45(1): 17-23. doi: 10.1016/s0025-326x(02)00227-8
    [4]
    强俊, 徐跑, 何杰, 等. 氨氮与拥挤胁迫对吉富品系尼罗罗非鱼幼鱼生长和肝脏抗氧化指标的联合影响[J]. 水产学报, 2011, 35(12): 1837-1848. doi: 10.3724/SP.J.1231.2011.17530
    [5]
    SUANYUK N, KONG F, KO D, et al. Occurrence of rare genotypes of Streptococcus agalactiae in cultured red tilapia Oreochromis sp. and Nile tilapia O. niloticus in Thailand relationship to human isolates[J]. Aquaculture, 2008, 284(1): 35-40. doi: 10.1016/J.AQUACULTURE.2008.07.034
    [6]
    祝璟琳, 杨弘, 邹芝英, 等. 海南养殖罗非鱼(Oreochromis niloticus)致病链球菌的分离、鉴定及其药敏试验[J]. 海洋与湖沼, 2010, 41(4): 590-596. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg5oeXloejIwMTAwNDAxORoIM3hzdzFkY2Y%3D
    [7]
    卢迈新. 罗非鱼病害防治和养殖技术进展[J]. 科学养鱼, 2010, 251: 110-112.
    [8]
    IP Y K, CHEW S F. Ammonia production, excretion, toxicity, and defense in fish: a review[J]. Front Physiol, 2010, 134(1): 1-20. doi: 10.3389/fphys.2010.00134
    [9]
    陈家长, 臧学磊, 胡庚东, 等. 氨氮胁迫下罗非鱼(GIFT Oreochromis niloticus)机体免疫力的变化及其对海豚链球菌易感性的影响[J]. 生态环境学报, 2011, 20(4): 629-634. doi: 10.3969/j.issn.1674-5906.2011.04.007
    [10]
    韩春艳, 郑清梅, 陈桂丹, 等. 氨氮胁迫对奥尼罗非鱼非特异性免疫的影响[J]. 南方水产科学, 2014, 10(3): 47-52. doi: 10.3969/j.issn.2095-0780.2014.03.007
    [11]
    中国环境保护局. 水和废水监测分析方法[M]. 北京: 中国环境科学出版社, 2002: 276-281.
    [12]
    黄建华, 李永, 杨其彬, 等. 斑节对虾家系氨氮耐受性的比较[J]. 南方水产科学, 2012, 8(6): 37-43. doi: 10.3969/j.issn.2095-0780.2012.06.006
    [13]
    FOSS A, SIIKAVUOPIO S I, SAETHER B S, et al. Effect of chronic ammonia exposure on growth in juvenile Atlantic cod[J]. Aquaculture, 2004, 237(1): 179-189. doi: 10.1016/J.AQUACULTURE.2004.03.013
    [14]
    PINTO W, ARAGAO C, SOARES F, et al. Growth, stress response and free amino acid levels in Senegalese sole (Solea senegalensis Kaup 1858) chronically exposed to exogenous ammonia[J]. Aquac Res, 2007, 38(11): 1198-1204. doi: 10.1111/j.1365-2109.2007.01788.x
    [15]
    FOSS A, IMSLAND A K, ROTH B, et al. Effects of chronic and periodic exposure to ammonia on growth and blood physiology in juvenile turbot (Scophthalmus maximus)[J]. Aquaculture, 2009, 296(1): 45-50. doi: 10.1016/J.AQUACULTURE.2009.07.013
    [16]
    PAUST L O, FOSS A, IMSLAND A K. Effects of chronic and periodic exposure to ammonia on growth, food conversion efficiency and blood physiology in juvenile Atlantic halibut (Hippoglossus hippoglossus L. )[J]. Aquaculture, 2011, 315(3): 400-406. doi: 10.1016/J.AQUACULTURE.2011.03.008
    [17]
    赵海鹏. 长吻血液生理、生化指标和血流变特性初步研究[D]. 重庆: 西南师范大学, 2005.
    [18]
    陶健芳, 刘来亭, 牛慧军, 等. 白头翁对草鱼免疫功能指标的影响[J]. 饲料研究, 2007(3): 52-54. doi: 10.3969/j.issn.1002-2813.2007.03.018
    [19]
    崔红红, 刘波, 戈贤平, 等. 肌醇对氨氮应激下团头鲂幼鱼免疫的影响[J]. 水产学报, 2014, 38(2): 228-236. https://d.wanfangdata.com.cn/conference/ChxDb25mZXJlbmNlTmV3UzIwMjQxMTEzMTU1MjI0Egc4Njk2MDA3Gghud3M2YWo4YQ%3D%3D
    [20]
    JENSEN F B. The role of nitrite in nitric oxide homeostasis: a comparative perspective[J]. Biochimica et Biophysica Acta, 2009, 1787: 841-848. doi: 10.1016/j.bbabio.2009.02.010
    [21]
    赵海涛. 氨氮对南方鲶幼鱼血液生理、生化及非特异性免疫指标的影响[D]. 重庆: 西南大学, 2006: 13-16.
    [22]
    班同, 尹晶, 吴垠. 氨氮浓度对工厂化养殖漠斑牙鲆血液指标的影响[J]. 河北渔业, 2013(8): 1-5. doi: 10.3969/j.issn.1004-6755.2013.08.001
    [23]
    廖金花, 陈巧, 林丽蓉, 等. 鲍鱼碱性磷酸酶的分离纯化和性质研究[J]. 厦门大学学报: 自然科学版, 2005, 44(2): 272-275. doi: 10.3321/j.issn:0438-0479.2005.02.030
    [24]
    李波. 氨氮和亚硝酸盐对黄颡鱼的毒性研究[D]. 武汉: 华中农业大学, 2010: 26-28.
    [25]
    牟海津, 江晓路, 刘树青, 等. 免疫多糖对栉孔扇贝酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶活性的影响[J]. 青岛海洋大学学报, 1999, 29(3): 463-468. doi: 10.1017/S0266078400010713
    [26]
    SUN H, WANG W, LI J, et al. Growth, oxidative stress responses, and gene transcription of juvenile bighead carp (Hypophthalmichthys nobilis) under chronic-term exposure of ammonia[J]. Environ Toxicol Chem, 2014, 33(8): 1726-1731. doi: 10.1002/etc.2613
    [27]
    蒋琦辰, 顾曙余, 张文逸, 等. 氨氮急性胁迫及其毒后恢复对红螯光壳螯虾幼虾相关免疫和代谢指标的影响[J]. 水产学报, 2013, 37(7): 1066-1072. doi: 10.3724/SP.J.1231.2013.38563
    [28]
    尹飞, 孙鹏, 彭士明, 等. 亚硝态氮和氨态氮急性胁迫下曼氏无针乌贼幼体血液的生化指标[J]. 生态毒理学报, 2011, 6(3): 289-295. http://hjhx.rcees.ac.cn/article/id/21567311-9245-4fff-a2bf-9f47fd7ebf57?viewType=HTML
    [29]
    LEMARIE G, DOSDAT A, COVES D, et al. Effect of chronic ammonia exposure on growth of European seabass(Dicentrarchus labrax) juveniles[J]. Aquaculture, 2004, 229(12/3/4): 479-491. doi: 10.1016/S0044-8486(03)00392-2
    [30]
    RUYET J P, LAMES A, ROUX A L, et al. Long-term ammonia exposure of turbot: effects on plasma parameters[J]. J Fish Biol, 2003, 62(4): 879-894. doi: 10.1046/j.1095-8649.2003.00073.x
    [31]
    DEWES L J, SANDRINI J Z, MONSERRAT J M, et al. Biochemical and physiological responses after exposure to microcystins in the crab Chasmagnathus granulatus(Decapoda Brachyura)[J]. Ecotox Environ Safe, 2006, 65(2) : 201-208. doi: 10.1016/j.ecoenv.2005.07.013. Epub 2005 Aug 31
    • Related Articles

  • Related Articles

    [1]HUANG Hao, FAN Sigang, WANG Pengfei, CHEN Jia, ZHAO Chao, YAN Lulu, QIU Lihua, PAN Ying. Genetic diversity analysis of six geographical populations of Lateolabrax maculatus based on microsatellite markers[J]. South China Fisheries Science, 2022, 18(1): 99-106. DOI: 10.12131/20210126
    [2]PENG Min, HAN Yaoquan, WANG Dapeng, SHI Jun, WU Weijun, LI Yusen, LEI Jianjun, HE Anyou. Genetic diversity analysis of Ptychidio jordani in Xijiang River flowing through Guangxi Province based on mitochondrial Cytb gene sequence[J]. South China Fisheries Science, 2020, 16(5): 10-18. DOI: 10.12131/20200041
    [3]KUANG Tianxu, SHUAI Fangmin, CHEN Weitao, LI Xinhui. Genetic diversity and population structure of Carassius auratus in Xijiang River[J]. South China Fisheries Science, 2018, 14(5): 29-35. DOI: 10.3969/j.issn.2095-0780.2018.05.004
    [4]CHEN Xinghan, GUO Liang, LI Mingming, MENG Zining, LIN Haoran. Analysis of RAPD and mitochondrial Cyt b gene sequences of three cultured stocks of Epinephelus coioides from Guangdong Province[J]. South China Fisheries Science, 2014, 10(4): 27-33. DOI: 10.3969/j.issn.2095-0780.2014.04.005
    [5]MO Yanxiu, WANG Xiaoqing, MO Yongliang. RAPD analysis of genetic diversity of Leiocassis longirostris[J]. South China Fisheries Science, 2010, 6(6): 77-80. DOI: 10.3969/j.issn.1673-2227.2010.06.014
    [6]YUAN Julin, ZHU Junjie, WANG Gaoxue. Analysis of the genetic diversity in Heihe River and Xushuihe River populations of Qinling Brachymystax lenok[J]. South China Fisheries Science, 2009, 5(3): 63-66. DOI: 10.3969/j.issn.1673-2227.2009.03.011
    [7]QU Youjun, WU Yong, LI Jiaer, LIU Chuwu. RAPD analysis of genetic difference among five species of grouper[J]. South China Fisheries Science, 2008, 4(2): 56-62.
    [8]LIU Jinli, CHEN Xinjun, XU Qianghua. Population genetic structure of Sthenoteuthis oualaniensis in the northwestern Indian Ocean by RAPD analysis[J]. South China Fisheries Science, 2008, 4(2): 43-49.
    [9]WANG Xiaoyu, YU Dahui, HUANG Guiju, GUO Yihui, DU Bo, GONG Shiyuan, WANG Aimin. Genetic segregation of AFLP markers and genetic diversity in three F1 families of pearl oyster Pinctada fucata[J]. South China Fisheries Science, 2007, 3(5): 54-60.
    [10]LI Lihao, YU Dahui, HUANG Guiju, DU Bo, FU Yun, TONG Xin, GUO Yihui, YE Wei. Genetic diversity of selected GIFT strains of Oreochromis niloticus[J]. South China Fisheries Science, 2007, 3(5): 40-48.

  • Cited by

    Periodical cited type(5)

    1. 申亚阳，杨铿，马红玲，冯娟，苏友禄，郭志勋. 广东沿海地区拟穴青蟹呼肠孤病毒和双顺反子病毒-1的分子流行病学调查. 生态科学. 2017(01): 17-24 .
    2. 崔亚婷，倪军，马红玲，许海东，冯娟，申亚阳，郭志勋. 青蟹双顺反子病毒-1原位杂交检测方法的建立与应用. 广东农业科学. 2015(11): 130-134+193 .
    3. 李淑娟，马洪雨，马春艳，蒋伟，冯娜娜，徐真，刘月星，乔振国，马凌波. AFLP分子标记在拟穴青蟹子一代家系中的遗传规律分析. 生物技术通报. 2013(11): 123-129 .
    4. 宋忠魁，李梦芸，聂振平，孙奉玉，赵鹏，苏琼. 北部湾拟穴青蟹(Scylla paramamosain)群体遗传结构及其扩张分析. 海洋与湖沼. 2012(04): 828-836 .
    5. 宋忠魁，孙奉玉，李梦芸，赵鹏，聂振平，苏琼. 北部湾6个拟穴青蟹群体遗传多样性的ISSR分析. 生态学杂志. 2012(10): 2585-2590 .

    Other cited types(4)

Catalog

    Get Citation
    PDF
    XML
    Article views (3402) PDF downloads (1845) Cited by(9)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return