Volume 10 Issue 5
Oct.  2014
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CHEN Yanfeng, ZHOU Aiguo, CHEN Guanfeng, CHEN Jianchou, ZHANG Jiping, ZHANG Huihua. Isolation of Edwardsiella tarda from cultured hybrid snakehead (Channa maculata♀×C.argus♂) and its identification[J]. South China Fisheries Science, 2014, 10(5): 1-7. DOI: 10.3969/j.issn.2095-0780.2014.05.001
Citation: CHEN Yanfeng, ZHOU Aiguo, CHEN Guanfeng, CHEN Jianchou, ZHANG Jiping, ZHANG Huihua. Isolation of Edwardsiella tarda from cultured hybrid snakehead (Channa maculata♀×C.argus♂) and its identification[J]. South China Fisheries Science, 2014, 10(5): 1-7. DOI: 10.3969/j.issn.2095-0780.2014.05.001
shu

Isolation of Edwardsiella tarda from cultured hybrid snakehead (Channa maculata♀×C.argus♂) and its identification

  • 1.

    College of Life Science, Foshan University, Foshan 528231, China

  • 2.

    College of Animal Science, South China Agricultural University, Guangzhou 510642, China

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  • Received Date: January 23, 2014
  • Revised Date: March 18, 2014
    Graphical Abstract
    • Abstract

  • Two pathogenic bacteria (ZS201364-1, ZS201364-2) isolated from diseased hybrid snakeheads (Channa maculata♀×C. argus♂) were identified to be Edwardsiella tarda by analyses of biochemical features and 16S rDNA sequence. The anaerobic, gram negative, bacilliform and motile isolates utilized glucoses and maltose as energy. Both indole and M.R. tests proved positive. The LD50s of the isolates to healthy hybrid snakeheads were 7.1×105 cfu · g-1 and 5.6×105 cfu · g-1, respectively. The optimal growth conditions of strain ZS201364-1, which was highly susceptible to cefotaxime, ciprofloxacin and norfloxacin, were 25~35 ℃ pH 7~8 and 1% NaCl.

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    • References (32)
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