Effects of several key environmental factors on survival of artificialbreeding of Onchidium struma

Based on simulate natural ecological environment, we conducted an experiment to explore the effects of several environmental factors such as temperature, salinity and relative humidity on the artificialbreeding of Onchidium struma. The results show that the survival reached over 90% and significant difference was observed among the other trail groups (P0.05) under the following conditions: temperature (20~26 ℃ with diurnal temperature range3 ℃), salinity (35), relative humidity (80%), stocking density 50 indtank-1 (333 indm-3), sediment (silty halosols on the surface of intertidal with 3 cm thickness laid at the bottom of the tank), light intensity (70 lx). It is revealed that thebreeding of O.struma needs control of temperature, salinity, relative humidity, stocking density, light intensity and sediment factors. Besides, the survival can be increased and long-term artificialbreeding can be achieved by simulatingbreeding environment and improvingbreeding conditions.