Volume 8 Issue 3
Jun.  2012
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Abstract
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SUN Xiumei, MEI Guangming, CHEN Xuechan, GUO Yuanming, CHEN Peng. Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence[J]. South China Fisheries Science, 2012, 8(3): 48-53. DOI: 10.3969/j.issn.2095-0780.2012.03.007
Citation: SUN Xiumei, MEI Guangming, CHEN Xuechan, GUO Yuanming, CHEN Peng. Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence[J]. South China Fisheries Science, 2012, 8(3): 48-53. DOI: 10.3969/j.issn.2095-0780.2012.03.007
shu

Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence

  • 1.

    Fishery Environment and Aquatic Products Quality Supervision Testing Center(Zhoushan), Ministry of Agriculture; Marine Fishery Institute of Zhejiang Province, Zhoushan 316100, China

  • 2.

    Sinomega Biotech Engineering Company, Zhoushan 316101, China

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  • Received Date: November 15, 2011
  • Revised Date: January 30, 2012
    Graphical Abstract
    • Abstract

  • We used high performance liquid chromatography (HPLC) coupled to a fluorescence detector (FLD) to determine 15 polycyclic aromatic hydrocarbons (PAHs) in aquatic products and realized complete base-line separation of the 15 PAHs and high sensitive fluorescence determination based on optimization of liquid chromatographic conditions, excitation and emission wavelength. Under optimizing conditions, the detection limit of naphthalene, acenaphthene and fluorene is 0.5 μg · kg-1; that of benzo (b) fluoranthene, dibenzo (a, h) anthracene and benzo (g, h, i) perylene is 2.0 μg · kg-1; that of the others is 1.0 μg · kg-1. PHE, AN, PY, BaA, CHR, BaP and IP are in the range of 0.001~0.500 mg · L-1, and the others are in the range of 0.002~1.000 mg · L-1, having good linearity (R2>0.995). The recovery at the spiked amount of 2.0 μg · kg-1, 10.0 μg · kg-1 and 100.0 μg · kg-1 is 71.1%~98.4% (RSD < 10%). The reproducible, sensitive and accurate method is suitable for quality control of the 15 PAHs in aquatic products.

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    • References (20)
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