Volume 8 Issue 3
Jun.  2012
Turn off MathJax
Article Contents
Abstract
References
Related Articles
SUN Xiumei, MEI Guangming, CHEN Xuechan, GUO Yuanming, CHEN Peng. Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence[J]. South China Fisheries Science, 2012, 8(3): 48-53. DOI: 10.3969/j.issn.2095-0780.2012.03.007
Citation: SUN Xiumei, MEI Guangming, CHEN Xuechan, GUO Yuanming, CHEN Peng. Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence[J]. South China Fisheries Science, 2012, 8(3): 48-53. DOI: 10.3969/j.issn.2095-0780.2012.03.007
shu

Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence

  • 1.

    Fishery Environment and Aquatic Products Quality Supervision Testing Center(Zhoushan), Ministry of Agriculture; Marine Fishery Institute of Zhejiang Province, Zhoushan 316100, China

  • 2.

    Sinomega Biotech Engineering Company, Zhoushan 316101, China

More Information
  • Received Date: November 15, 2011
  • Revised Date: January 30, 2012
    Graphical Abstract
    • Abstract

  • We used high performance liquid chromatography (HPLC) coupled to a fluorescence detector (FLD) to determine 15 polycyclic aromatic hydrocarbons (PAHs) in aquatic products and realized complete base-line separation of the 15 PAHs and high sensitive fluorescence determination based on optimization of liquid chromatographic conditions, excitation and emission wavelength. Under optimizing conditions, the detection limit of naphthalene, acenaphthene and fluorene is 0.5 μg · kg-1; that of benzo (b) fluoranthene, dibenzo (a, h) anthracene and benzo (g, h, i) perylene is 2.0 μg · kg-1; that of the others is 1.0 μg · kg-1. PHE, AN, PY, BaA, CHR, BaP and IP are in the range of 0.001~0.500 mg · L-1, and the others are in the range of 0.002~1.000 mg · L-1, having good linearity (R2>0.995). The recovery at the spiked amount of 2.0 μg · kg-1, 10.0 μg · kg-1 and 100.0 μg · kg-1 is 71.1%~98.4% (RSD < 10%). The reproducible, sensitive and accurate method is suitable for quality control of the 15 PAHs in aquatic products.

    • FullText(HTML)
    • References (20)
  • [1]
    冯承莲, 夏星辉, 周追, 等. 长江武汉段水体中多环芳烃的分布及来源分析[J]. 环境科学学报, 2007, 27(11): 1900-1908. doi: 10.3321/j.issn:0253-2468.2007.11.025

    FENG Chenglian, XIA Xinghui, ZHOU Zhui, et al. Distribution and sources of polycyclic aromatic hydrocarbons in the Wuhan section of the Yangtze River[J]. Acta Scientiae Circumstantiae, 2007, 27(11): 1900-1908. (in Chinese) doi: 10.3321/j.issn:0253-2468.2007.11.025
    [2]
    钱薇, 倪进治, 骆永明, 等. 高效液相色谱-荧光检测法测定土壤中的多环芳烃[J]. 色谱, 2007, 25(2): 221-225. doi: 10.3321/j.issn:1000-8713.2007.02.020

    QIAN Wei, NI Jinzhi, LUO Yongming, et al. Determination of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with fluorescence detection[J]. Chin J Chromatogr, 2007, 25(2): 221-225. (in Chinese) doi: 10.3321/j.issn:1000-8713.2007.02.020
    [3]
    丁潇, 白志鹏, 韩斌, 等. 鞍山市大气PM10中多环芳烃(PAHs)的污染特征及其来源[J]. 环境科学研究, 2011, 24(2): 162-171. https://www.zhangqiaokeyan.com/academic-journal-cn_research-environmental-sciences_thesis/0201217566688.html

    DING Xiao, BAI Zhipeng, HAN Bin, et al. Pollution characteristics and source analysis of PAHs in PM10 in Anshan city[J]. Res Environ Sci, 2011, 24(2): 162-171. (in Chinese) https://www.zhangqiaokeyan.com/academic-journal-cn_research-environmental-sciences_thesis/0201217566688.html
    [4]
    冯克良. 概述多环芳烃在自然环境中的分布与监测[J]. 海洋环境科学, 1991, 10(4): 74-76. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg5RSzAwMDAwMTUzNDU4MBoIc3g4emxnNm4%3D

    FENG Keliang. Distribution and monitoring of polycyclic aromatic hydrocarbons in natural environment[J]. Mar Environ Sci, 1991, 10(4): 74-76. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg5RSzAwMDAwMTUzNDU4MBoIc3g4emxnNm4%3D
    [5]
    BOLS N C, BRUBACHER J L, GANASSIN R C, et al. Ecotoxicology and innate immunity in fish[J]. Dev Comp Immunol, 2001, 25(8/9): 853-873. doi: 10.1016/S0145-305X(01)00040-4
    [6]
    VIGANÒ L, CAMOIRANO A, IZZOTTI A, et al. Mutagenicity of sediments along the Po River and genotoxicity biomarkers in fish from polluted areas[J] Mutat Res, 2002, 515(1/2): 125-134. doi: 10.1016/s1383-5718(02)00002-5
    [7]
    CLARK B W, MATSON C W, JUNQ D, et al. AHR2 mediates cardiac teratogenesis of polycyclic aromatic hydrocarbons and PCB-126 in Atlantic killifish (Fundulus heteroclitus)[J]. Aquat Toxicol, 2010, 99(2): 232-240. doi: 10.1016/j.aquatox.2010.05.004
    [8]
    廖倩, 高振江, 张世湘, 等. 高效液相荧光法测定"北京烤鸭"鸭皮中的多环芳烃[J]. 食品科学, 2009, 30(10): 149-152. doi: 10.7506/spkx1002-6630-200910029

    LIAO Qian, GAO Zhenjiang, ZHANG Shixiang, et al. Determination of polycyclic aromatic hydrocarbons in skin of "Peking roast duck" by high performance chromatography-fluorescence detection[J]. Food Sci, 2009, 30(10): 149-152. (in Chinese) doi: 10.7506/spkx1002-6630-200910029
    [9]
    SARRAZIN L, DIANA C, SCHEMBRI T, et al. HPLC determination of polycyclic aromatic hydrocarbons (PAHs) in the littoral urban sewage area of Cortiou (Marseille, France)[J]. Int J Environ Stud, 2004, 61(4): 413-425. doi: 10.1080/00207230410001693110
    [10]
    SIMKO P. Determination of polycyclic aromatic hydrocarbons in smoked meat products and smoke flavouring food additives[J]. J Chromatogr B, 2002, 770(1/2): 3-18. doi: 10.1016/S0378-4347(01)00438-8
    [11]
    CHUNG M K, RIBY J, LI He, et al. A sandwich enzyme-linked immunosorbent assay for adducts of polycyclic aromatic hydrocarbons with human serum albumin[J]. Anal Biochem, 2010, 400(1): 123-129. doi: 10.1016/j.ab.2010.01.018
    [12]
    饶竹, 李松, 何淼, 等. 高效液相色谱-荧光-紫外串联测定土壤中16种多环芳烃[J]. 分析化学, 2007, 35(7): 954-958. doi: 10.3321/j.issn:0253-3820.2007.07.005

    RAO Zhu, LI Song, HE Miao, et al. Determination of 16 polycyclic aromatic hydrocarbaons in soil by high performance liquid chromatography with fluorescence and ultraviolet detection[J]. Chin J Analyt Chem, 2007, 35(7): 954-958. (in Chinese) doi: 10.3321/j.issn:0253-3820.2007.07.005
    [13]
    郭丽, 惠亚梅, 郑明辉, 等. 气相色谱-质谱联用测定土壤及底泥样品中的多环芳烃和硝基多环芳烃[J]. 环境化学, 2007, 26(2): 192-196. http://hjhx.rcees.ac.cn/article/id/hjhx_1111

    GUO Li, HUI Yamei, ZHENG Minghui, et al. Determination of polycyclic aromatic hydrocarbons and nitro-polycyclic aromatic hydrocarbons in soil and sediment by gas chromatography-mass spectrometry[J]. Environ Chem, 2007, 26(2): 192-196. (in Chinese) http://hjhx.rcees.ac.cn/article/id/hjhx_1111
    [14]
    MIRSADEGHI S A, ZAKARIA M P, YAP C K, et al. Risk assessment for the daily intake of polycyclic aromatic hydrocarbons from the ingestion of cockle (Anadara granosa) and exposure to contaminated water and sediments along the west coast of Peninsular Malaysia[J]. J Environ Sci, 2011, 23(2): 336-345. doi: 10.1016/S1001-0742(10)60411-1
    [15]
    杨家锋, 钟惠英, 段青源, 等. 气相色谱-质谱联用法测定水产品中16种多环芳烃[J]. 中国卫生检验杂志, 2009, 19(10): 2291-2295. https://d.wanfangdata.com.cn/periodical/zgwsjyzz200910033

    YANG Jiafeng, ZHONG Huiying, DUAN Qingyuan, et al. Determination of 16 polycyclic aromatic hydrocarbons in aquatic products by GC/MS[J]. Chin J Health Lab Tech, 2009, 19(10): 2291-2295. (in Chinese) https://d.wanfangdata.com.cn/periodical/zgwsjyzz200910033
    [16]
    钟惠英, 郑丹, 杨家锋, 等. 中华人民共和国水产行业标准——SC/T 3042-2008水产品中16种多环芳烃的测定: 气相色谱-质谱法[S]. 北京: 中国农业出版社, 2008. http://down.foodmate.net/standard/sort/7/17534.html

    ZHONG Huiying, ZHENG Dan, YANG Jiafeng, et al, Aquaculture industry standards of People's Republic of China: SC/T 3042-2008 determination of 16 polycyclic aromatic hydrocarbons in aquatic products gas chromatography-mass spectrometry[S]. Beijing: China Agriculture Press, 2008. (in Chinese) http://down.foodmate.net/standard/sort/7/17534.html
    [17]
    方杰, 王凯雄. 气象色谱-离子阱质谱法测定海洋贝类中多残留有机氯农药、多氯联苯和多环芳烃[J]. 分析化学, 2007, 35(11): 1607-1613, doi: 10.3321/j.issn:0253-3820.2007.11.013

    FANG Jie, WANG Kaixiong. Multiresidual analysis of organochlorine pesticides, polychlorinated biphenyls and polycyclic aromatic hydrocarbons in marine shellfishes by gas chromatography-ion trap mass spectrometry[J]. Chin J Analyt Chem, 2007, 35(11): 1607-1613. (in Chinese) doi: 10.3321/j.issn:0253-3820.2007.11.013
    [18]
    芮昶, 李康, 李玮, 等. 气相色谱-质谱法测定烤鱼片中16种多环芳烃[J]. 质谱学报, 2008, 29(1): 30-35. doi: 10.3724/SP.J.1011.2008.00529

    RUI Chang, LI Kang, LI Wei, et al. Determination of polycylic aromatic hydrocarbons in baked fillet by GC/MS[J]. J Chin Mass Spectro Soc, 2008, 29(1): 30-35. (in Chinese) doi: 10.3724/SP.J.1011.2008.00529
    [19]
    吴雪美, 张虹, 戴志远, 等. 高效液相色谱法分析食品中多环芳香类物质(PAHs)[J]. 中国粮油学报, 2008, 23(3): 189-193. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg96Z2x5eGIyMDA4MDMwNDQaCGZ0cjIydHEz

    WU Xuemei, ZHANG Hong, DAI Zhiyuan, et al. Determination of PAHs in food by high performance liquid chromatography[J]. J Chin Cereals Oil Assoc, 2008, 23(3): 189-193. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg96Z2x5eGIyMDA4MDMwNDQaCGZ0cjIydHEz
    [20]
    ZHANG Hong, XUE Ming, DAI Zhiyuan. Determination of polycyclic aromatic hydrocarbons in aquatic products by HPLC-fluorescence[J]. J Food Comp Anal, 2010, 23(5): 469-474. doi: 10.1016/j.jfca.2009.12.016
    • Related Articles

  • Related Articles

    [1]LI Xue, LI Yafang, CHEN Qinghua, NING Jiajia, WANG Lianggen, XU Lei, HUANG Delian, LIU Shuangshuang, ZHONG Chao, ZHANG Jian, WANG Xuehui, DU Feiyan. Structural characteristics and relative influencing factors of macrobenthos community in intertidal zone of Daya Bay[J]. South China Fisheries Science, 2023, 19(3): 42-50. DOI: 10.12131/20220202
    [2]WANG Teng, LIU Yong, QUAN Qiumei, LIN Lin, XIAO Yayuan, LI Chunhou, LI Hong. Community structure characteristics of zooplankton in main freshwater rivers of Jiangmen City, Guangdong Province[J]. South China Fisheries Science, 2021, 17(4): 9-17. DOI: 10.12131/20210019
    [3]CHEN Zilin, LI Chunhou, XIAO Yayuan, LIU Yong, LIN Lin, WANG Jiujiang, QUAN Qiumei. Distribution of structure of macrobenthic communities in coastal waters of Jiangmen, Guangdong[J]. South China Fisheries Science, 2020, 16(4): 18-27. DOI: 10.12131/20190248
    [4]GONG Yuyan, XIAO Yayuan, XU Shannan, LIU Yong, YANG Yutao, HUANG Zirong, LI Chunhou. Zooplankton community structure in Hailing Bay and its relationship with primary environmental factors[J]. South China Fisheries Science, 2019, 15(6): 49-55. DOI: 10.12131/20180220
    [5]WANG Lianggen, LI Yafang, DU Feiyan, NING Jiajia, XU Lei, XIAO Yayuan, LI Chunhou. Zooplankton community characteristics and impact on ichthyoplankton in artificial reefs and island reefs of Daya Bay[J]. South China Fisheries Science, 2018, 14(2): 41-50. DOI: 10.3969/j.issn.2095-0780.2018.02.006
    [6]LÜ Shaoliang, WANG Xuefeng, ZENG Jiawei, CHEN Haigang, WANG Lifei, JIA Xiaoping. Phytoplankton community structure and its environmental adaptation in Fangcheng coastal waters[J]. South China Fisheries Science, 2017, 13(4): 17-25. DOI: 10.3969/j.issn.2095-0780.2017.04.003
    [7]DENG Bangping, XU Ren, LIU Caicai, CAI Peng, YE Shufeng. Distribution characteristics of zooplankton communities in offshore waters of the Southern Yellow Sea and the East China Sea in summer[J]. South China Fisheries Science, 2015, 11(4): 11-19. DOI: 10.3969/j.issn.2095-0780.2015.04.002
    [8]HU Chengye, XU Heng, SHUI Bonian, HAN Zhiqiang, DU Xiao, WANG Fengli, ZHENG Wei, LI Liang. Community structure and biodiversity of nekton near Oufei Beach of Wenzhou[J]. South China Fisheries Science, 2015, 11(3): 7-15. DOI: 10.3969/j.issn.2095-0780.2015.03.002
    [9]DENG Bangping, CHEN Dong, JI Huanhong, WANG Jinhui, XU Ren. Ecological response of zooplankton community to tidal movement in Fengxian coastal area in autumn[J]. South China Fisheries Science, 2011, 7(1): 69-72. DOI: 10.3969/j.issn.2095-0780.2011.01.011
    [10]GAO Yuan, LAI Zini, WANG Chao, PANG Shixun, WEI Taili, XIE Wenping, YANG Wanling. Community characteristics of zooplankton in Pearl River Estuary in summer of 2006[J]. South China Fisheries Science, 2008, 4(1): 10-15.

  • Cited by

    Periodical cited type(30)

    1. 初建松，郑卫东，孙利元，彭道民，索安宁，朱玉贵. 基于生物资源的万山海洋牧场生境适宜性评估. 海洋科学. 2024(01): 75-84 .
    2. 田思泉，柳晓雪，花传祥，王寅，杜涣洋. 南海渔业资源状况及其管理挑战. 上海海洋大学学报. 2024(03): 786-798 .
    3. 李炳楠，胡梓阳，董建宇，吕少梁，陈宁，初庆柱，王学锋. 海南东部海域甲壳类群落结构特征及其环境影响因子. 广东海洋大学学报. 2024(06): 50-58 .
    4. 蔡研聪，孙铭帅，许友伟，陈作志. 南海北部近海中国枪乌贼分布与海洋环境关系的空间异质研究. 南方水产科学. 2023(03): 1-10 . 本站查看
    5. 刘锴，马嘉昕. 中国海洋渔业碳汇的时空演变及发展态势. 资源开发与市场. 2023(07): 778-785 .
    6. 刘彩莲，许庆，王林龙，邢衍阔，宋稼豪，林柏岸，康斌，刘敏. 闽东近海春秋季游泳动物多样性、密度及群落特征. 生物多样性. 2023(07): 115-131 .
    7. 粟丽，许友伟，张魁，陈作志. 南海区拖网渔业发展趋势及其对渔业资源的影响. 南方水产科学. 2023(04): 41-48 . 本站查看
    8. 冯瑞玉，郭禹，李金明，孙金辉，于刚，吴一桂，秦传新. 基于EnhanceFish模型的鱼类增殖放流策略研究:以中山市南朗水域黄鳍棘鲷增殖放流为例. 渔业科学进展. 2023(05): 1-10 .
    9. 罗峥力，杨长平，王良明，刘岩，单斌斌，刘蔓婷，孙典荣. 北部湾北部沿岸海域鱼类资源时空分布特征及多样性变化. 南方农业学报. 2023(06): 1847-1857 .
    10. 裴精花，陈清华，范金金，刘伟杰，郭照良，隋昊志. 珠江口海域游泳动物群落结构及多样性特征. 南方农业学报. 2023(12): 3727-3738 .
    11. 麦广铭，陈志劼，王学锋，肖雅元，李纯厚. 南海北部沿岸鱼类分类学多样性的空间格局. 南方水产科学. 2022(03): 38-47 . 本站查看
    12. 潘士远，林坤，吕少梁，王学锋. 种间互作对雷州湾海域鱼类群落结构的影响. 生态学报. 2022(22): 9383-9393 .
    13. 周卫国，丁德文，索安宁，何伟宏，田涛. 珠江口海洋牧场渔业资源关键功能群的遴选方法. 水产学报. 2021(03): 433-443 .
    14. 刘前，王学锋，吕少梁，林坤，张静. 湛江港海域游泳动物群落结构及多样性分析. 广东海洋大学学报. 2021(02): 103-110 .
    15. 张羽翔，刘维，赵海龙，吴程宏，陈敏. 海南岛近岸春秋季游泳动物多样性分析. 渔业研究. 2021(02): 133-142 .
    16. 徐鹏，谢木娇，周卫国，孙英婷，丁德文，索安宁. 近30年珠江口海域游泳动物经济物种群落结构变化特征. 应用海洋学学报. 2021(02): 239-250 .
    17. 易晓英，邱康文，周霄，招春旭，邓裕坚，何雄波，颜云榕. 北部湾斑鳍白姑鱼渔业生物学分析. 上海海洋大学学报. 2021(03): 515-524 .
    18. 陆丽仪，曾嘉维，林坤，张静，王学锋. 海南东部外海海域鱼类群落结构季节变动及其影响因素. 广东海洋大学学报. 2021(03): 28-35 .
    19. 于杰，周艳波，唐振朝，陈国宝，曾雷. 基于北斗卫星船位监控系统数据的南海双拖渔船作业特征研究. 海洋科学. 2021(10): 40-48 .
    20. 余文全. 南海渔业冲突的不同情境与政策选择. 东南亚研究. 2021(04): 106-123+154 .
    21. 李敏，孔啸兰，许友伟，陈作志. 基于线粒体控制区序列的花斑蛇鲻遗传多态性分析. 热带海洋学报. 2020(04): 42-49 .
    22. 于南京，俞存根，菅康康，许永久，孙蓓蓓，张佩怡，刘坤，王慧君. 嵊泗列岛邻近海域鱼类种类组成及多样性分析. 渔业研究. 2020(04): 293-301 .
    23. 蔡研聪，孙铭帅，许友伟，李佳俊，黄梓荣，陈作志，杨长平，刘维达，张衡. 南海北部近海头足类优势种及其生态位特征. 应用生态学报. 2020(08): 2793-2803 .
    24. 彭露，徐姗楠，蔡研聪，许友伟，孙铭帅，陈作志，张魁，粟丽. 北部湾金线鱼的数量分布及变化趋势. 海洋湖沼通报. 2020(04): 120-126 .
    25. 蔡研聪，黄梓荣，李佳俊，许友伟，孙铭帅，陈作志，刘维达. 南海北部近海新记录种——苏门答腊金线鱼资源分布特征. 南方水产科学. 2020(06): 1-11 . 本站查看
    26. 曾嘉维，林坤，王学锋，李纯厚. 雷州湾附近海域鱼类群落结构及其与环境因子的关系. 中国水产科学. 2019(01): 108-117 .
    27. 刘祝楠，余景，陈丕茂，于杰，王言丰. 粤西海域灯光罩网渔场时空分布与海洋环境的关系. 南方农业学报. 2019(04): 867-874 .
    28. 蔡研聪，黄梓荣，许友伟，孙铭帅，徐姗楠，张魁，陈作志. 南海北部近海渔业资源密度概率分布特征. 应用生态学报. 2019(07): 2426-2436 .
    29. 王言丰，余景，陈丕茂，于杰，刘祝楠. 北部湾灯光罩网渔场时空分布与海洋环境关系分析. 热带海洋学报. 2019(05): 68-76 .
    30. Liu Zhunan，Yu Jing. Response of Spatio-temporal Distribution of Fishery Resources to Mairne Environment in the Northern South China Sea. Animal Husbandry and Feed Science. 2018(Z1): 311-317+320 .

    Other cited types(16)

Catalog

    Get Citation
    PDF
    XML
    Article views (4055) PDF downloads (2702) Cited by(46)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return