Volume 8 Issue 3
Jun.  2012
Turn off MathJax
Article Contents
Abstract
References
Related Articles
SUN Xiumei, MEI Guangming, CHEN Xuechan, GUO Yuanming, CHEN Peng. Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence[J]. South China Fisheries Science, 2012, 8(3): 48-53. DOI: 10.3969/j.issn.2095-0780.2012.03.007
Citation: SUN Xiumei, MEI Guangming, CHEN Xuechan, GUO Yuanming, CHEN Peng. Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence[J]. South China Fisheries Science, 2012, 8(3): 48-53. DOI: 10.3969/j.issn.2095-0780.2012.03.007
shu

Determination of 15 polycyclic aromatic hydrocarbons in aquatic products by HPLC-Fluorescence

  • 1.

    Fishery Environment and Aquatic Products Quality Supervision Testing Center(Zhoushan), Ministry of Agriculture; Marine Fishery Institute of Zhejiang Province, Zhoushan 316100, China

  • 2.

    Sinomega Biotech Engineering Company, Zhoushan 316101, China

More Information
  • Received Date: November 15, 2011
  • Revised Date: January 30, 2012
    Graphical Abstract
    • Abstract

  • We used high performance liquid chromatography (HPLC) coupled to a fluorescence detector (FLD) to determine 15 polycyclic aromatic hydrocarbons (PAHs) in aquatic products and realized complete base-line separation of the 15 PAHs and high sensitive fluorescence determination based on optimization of liquid chromatographic conditions, excitation and emission wavelength. Under optimizing conditions, the detection limit of naphthalene, acenaphthene and fluorene is 0.5 μg · kg-1; that of benzo (b) fluoranthene, dibenzo (a, h) anthracene and benzo (g, h, i) perylene is 2.0 μg · kg-1; that of the others is 1.0 μg · kg-1. PHE, AN, PY, BaA, CHR, BaP and IP are in the range of 0.001~0.500 mg · L-1, and the others are in the range of 0.002~1.000 mg · L-1, having good linearity (R2>0.995). The recovery at the spiked amount of 2.0 μg · kg-1, 10.0 μg · kg-1 and 100.0 μg · kg-1 is 71.1%~98.4% (RSD < 10%). The reproducible, sensitive and accurate method is suitable for quality control of the 15 PAHs in aquatic products.

    • FullText(HTML)
    • References (20)
  • [1]
    冯承莲, 夏星辉, 周追, 等. 长江武汉段水体中多环芳烃的分布及来源分析[J]. 环境科学学报, 2007, 27(11): 1900-1908. doi: 10.3321/j.issn:0253-2468.2007.11.025

    FENG Chenglian, XIA Xinghui, ZHOU Zhui, et al. Distribution and sources of polycyclic aromatic hydrocarbons in the Wuhan section of the Yangtze River[J]. Acta Scientiae Circumstantiae, 2007, 27(11): 1900-1908. (in Chinese) doi: 10.3321/j.issn:0253-2468.2007.11.025
    [2]
    钱薇, 倪进治, 骆永明, 等. 高效液相色谱-荧光检测法测定土壤中的多环芳烃[J]. 色谱, 2007, 25(2): 221-225. doi: 10.3321/j.issn:1000-8713.2007.02.020

    QIAN Wei, NI Jinzhi, LUO Yongming, et al. Determination of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with fluorescence detection[J]. Chin J Chromatogr, 2007, 25(2): 221-225. (in Chinese) doi: 10.3321/j.issn:1000-8713.2007.02.020
    [3]
    丁潇, 白志鹏, 韩斌, 等. 鞍山市大气PM10中多环芳烃(PAHs)的污染特征及其来源[J]. 环境科学研究, 2011, 24(2): 162-171. https://www.zhangqiaokeyan.com/academic-journal-cn_research-environmental-sciences_thesis/0201217566688.html

    DING Xiao, BAI Zhipeng, HAN Bin, et al. Pollution characteristics and source analysis of PAHs in PM10 in Anshan city[J]. Res Environ Sci, 2011, 24(2): 162-171. (in Chinese) https://www.zhangqiaokeyan.com/academic-journal-cn_research-environmental-sciences_thesis/0201217566688.html
    [4]
    冯克良. 概述多环芳烃在自然环境中的分布与监测[J]. 海洋环境科学, 1991, 10(4): 74-76. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg5RSzAwMDAwMTUzNDU4MBoIc3g4emxnNm4%3D

    FENG Keliang. Distribution and monitoring of polycyclic aromatic hydrocarbons in natural environment[J]. Mar Environ Sci, 1991, 10(4): 74-76. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg5RSzAwMDAwMTUzNDU4MBoIc3g4emxnNm4%3D
    [5]
    BOLS N C, BRUBACHER J L, GANASSIN R C, et al. Ecotoxicology and innate immunity in fish[J]. Dev Comp Immunol, 2001, 25(8/9): 853-873. doi: 10.1016/S0145-305X(01)00040-4
    [6]
    VIGANÒ L, CAMOIRANO A, IZZOTTI A, et al. Mutagenicity of sediments along the Po River and genotoxicity biomarkers in fish from polluted areas[J] Mutat Res, 2002, 515(1/2): 125-134. doi: 10.1016/s1383-5718(02)00002-5
    [7]
    CLARK B W, MATSON C W, JUNQ D, et al. AHR2 mediates cardiac teratogenesis of polycyclic aromatic hydrocarbons and PCB-126 in Atlantic killifish (Fundulus heteroclitus)[J]. Aquat Toxicol, 2010, 99(2): 232-240. doi: 10.1016/j.aquatox.2010.05.004
    [8]
    廖倩, 高振江, 张世湘, 等. 高效液相荧光法测定"北京烤鸭"鸭皮中的多环芳烃[J]. 食品科学, 2009, 30(10): 149-152. doi: 10.7506/spkx1002-6630-200910029

    LIAO Qian, GAO Zhenjiang, ZHANG Shixiang, et al. Determination of polycyclic aromatic hydrocarbons in skin of "Peking roast duck" by high performance chromatography-fluorescence detection[J]. Food Sci, 2009, 30(10): 149-152. (in Chinese) doi: 10.7506/spkx1002-6630-200910029
    [9]
    SARRAZIN L, DIANA C, SCHEMBRI T, et al. HPLC determination of polycyclic aromatic hydrocarbons (PAHs) in the littoral urban sewage area of Cortiou (Marseille, France)[J]. Int J Environ Stud, 2004, 61(4): 413-425. doi: 10.1080/00207230410001693110
    [10]
    SIMKO P. Determination of polycyclic aromatic hydrocarbons in smoked meat products and smoke flavouring food additives[J]. J Chromatogr B, 2002, 770(1/2): 3-18. doi: 10.1016/S0378-4347(01)00438-8
    [11]
    CHUNG M K, RIBY J, LI He, et al. A sandwich enzyme-linked immunosorbent assay for adducts of polycyclic aromatic hydrocarbons with human serum albumin[J]. Anal Biochem, 2010, 400(1): 123-129. doi: 10.1016/j.ab.2010.01.018
    [12]
    饶竹, 李松, 何淼, 等. 高效液相色谱-荧光-紫外串联测定土壤中16种多环芳烃[J]. 分析化学, 2007, 35(7): 954-958. doi: 10.3321/j.issn:0253-3820.2007.07.005

    RAO Zhu, LI Song, HE Miao, et al. Determination of 16 polycyclic aromatic hydrocarbaons in soil by high performance liquid chromatography with fluorescence and ultraviolet detection[J]. Chin J Analyt Chem, 2007, 35(7): 954-958. (in Chinese) doi: 10.3321/j.issn:0253-3820.2007.07.005
    [13]
    郭丽, 惠亚梅, 郑明辉, 等. 气相色谱-质谱联用测定土壤及底泥样品中的多环芳烃和硝基多环芳烃[J]. 环境化学, 2007, 26(2): 192-196. http://hjhx.rcees.ac.cn/article/id/hjhx_1111

    GUO Li, HUI Yamei, ZHENG Minghui, et al. Determination of polycyclic aromatic hydrocarbons and nitro-polycyclic aromatic hydrocarbons in soil and sediment by gas chromatography-mass spectrometry[J]. Environ Chem, 2007, 26(2): 192-196. (in Chinese) http://hjhx.rcees.ac.cn/article/id/hjhx_1111
    [14]
    MIRSADEGHI S A, ZAKARIA M P, YAP C K, et al. Risk assessment for the daily intake of polycyclic aromatic hydrocarbons from the ingestion of cockle (Anadara granosa) and exposure to contaminated water and sediments along the west coast of Peninsular Malaysia[J]. J Environ Sci, 2011, 23(2): 336-345. doi: 10.1016/S1001-0742(10)60411-1
    [15]
    杨家锋, 钟惠英, 段青源, 等. 气相色谱-质谱联用法测定水产品中16种多环芳烃[J]. 中国卫生检验杂志, 2009, 19(10): 2291-2295. https://d.wanfangdata.com.cn/periodical/zgwsjyzz200910033

    YANG Jiafeng, ZHONG Huiying, DUAN Qingyuan, et al. Determination of 16 polycyclic aromatic hydrocarbons in aquatic products by GC/MS[J]. Chin J Health Lab Tech, 2009, 19(10): 2291-2295. (in Chinese) https://d.wanfangdata.com.cn/periodical/zgwsjyzz200910033
    [16]
    钟惠英, 郑丹, 杨家锋, 等. 中华人民共和国水产行业标准——SC/T 3042-2008水产品中16种多环芳烃的测定: 气相色谱-质谱法[S]. 北京: 中国农业出版社, 2008. http://down.foodmate.net/standard/sort/7/17534.html

    ZHONG Huiying, ZHENG Dan, YANG Jiafeng, et al, Aquaculture industry standards of People's Republic of China: SC/T 3042-2008 determination of 16 polycyclic aromatic hydrocarbons in aquatic products gas chromatography-mass spectrometry[S]. Beijing: China Agriculture Press, 2008. (in Chinese) http://down.foodmate.net/standard/sort/7/17534.html
    [17]
    方杰, 王凯雄. 气象色谱-离子阱质谱法测定海洋贝类中多残留有机氯农药、多氯联苯和多环芳烃[J]. 分析化学, 2007, 35(11): 1607-1613, doi: 10.3321/j.issn:0253-3820.2007.11.013

    FANG Jie, WANG Kaixiong. Multiresidual analysis of organochlorine pesticides, polychlorinated biphenyls and polycyclic aromatic hydrocarbons in marine shellfishes by gas chromatography-ion trap mass spectrometry[J]. Chin J Analyt Chem, 2007, 35(11): 1607-1613. (in Chinese) doi: 10.3321/j.issn:0253-3820.2007.11.013
    [18]
    芮昶, 李康, 李玮, 等. 气相色谱-质谱法测定烤鱼片中16种多环芳烃[J]. 质谱学报, 2008, 29(1): 30-35. doi: 10.3724/SP.J.1011.2008.00529

    RUI Chang, LI Kang, LI Wei, et al. Determination of polycylic aromatic hydrocarbons in baked fillet by GC/MS[J]. J Chin Mass Spectro Soc, 2008, 29(1): 30-35. (in Chinese) doi: 10.3724/SP.J.1011.2008.00529
    [19]
    吴雪美, 张虹, 戴志远, 等. 高效液相色谱法分析食品中多环芳香类物质(PAHs)[J]. 中国粮油学报, 2008, 23(3): 189-193. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg96Z2x5eGIyMDA4MDMwNDQaCGZ0cjIydHEz

    WU Xuemei, ZHANG Hong, DAI Zhiyuan, et al. Determination of PAHs in food by high performance liquid chromatography[J]. J Chin Cereals Oil Assoc, 2008, 23(3): 189-193. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg96Z2x5eGIyMDA4MDMwNDQaCGZ0cjIydHEz
    [20]
    ZHANG Hong, XUE Ming, DAI Zhiyuan. Determination of polycyclic aromatic hydrocarbons in aquatic products by HPLC-fluorescence[J]. J Food Comp Anal, 2010, 23(5): 469-474. doi: 10.1016/j.jfca.2009.12.016
    • Related Articles

  • Related Articles

    [1]LU Hongyi, TIAN Haifeng, HU Qiaomu, LI Zhong. Parentage assignment of Monopterus albus using multiplex PCR of microsatallites[J]. South China Fisheries Science, 2023, 19(6): 97-106. DOI: 10.12131/20230081
    [2]LI Min, LI Yongfu, HUANG Yuhao, CHEN Zhuojun, MO Zuanlan, ZHONG Qunfang, LI Benwang, ZHANG Xianpeng. Establishment of reverse transcription droplet digital PCR assay for detection of Tilapia Lake Virus[J]. South China Fisheries Science, 2023, 19(1): 75-85. DOI: 10.12131/20220184
    [3]SHEN Fei, ZHAI Yufei, WANG Hao, LYU Liqun. Antimicrobial spectrum, resistance gene detection and ERIC-PCR genotyping of Vibrio scophthalmi[J]. South China Fisheries Science, 2022, 18(1): 118-127. DOI: 10.12131/20210138
    [4]MENG Delong, SHEN Benlong, BAI Wanqiang, XUE Baobao, SHEN Heding. Cloning and tissue expression of heat shock transcription factor 1 (HSF1) gene of Sinonovacula constricta[J]. South China Fisheries Science, 2020, 16(5): 115-122. DOI: 10.12131/20190164
    [5]FU Mingjun, ZHAO Chao, ZHOU Falin, QIU Lihua, JIANG Shigui. Molecular cloning and expression analysis of Ubiquitin-conjugating enzyme gene from black tiger shrimp (Penaeus monodon)[J]. South China Fisheries Science, 2015, 11(6): 41-48. DOI: 10.3969/j.issn.2095-0780.2015.06.006
    [6]WANG Rui, LI Liping, HUANG Ting, LIANG Wanwen, LIANG Cong, LEI Aiying, CHEN Ming. Real-time quantitative PCR for detection of Streptococcus agalactiae from tilapia tissue[J]. South China Fisheries Science, 2015, 11(3): 41-46. DOI: 10.3969/j.issn.2095-0780.2015.03.007
    [7]LONG Minming, HUANG Guiju, ZOU Jixing, GUO Yihui, FAN Sigang, LIU Baosuo, YU Dahui. Effect of pearl culture on N19 and Prismalin-14 genes expression in the pearl oyster Pinctada fucata[J]. South China Fisheries Science, 2013, 9(5): 58-63. DOI: 10.3969/j.issn.2095-0780.2013.05.010
    [8]LAO Haihua, YE Xing, ZOU Weimin, BAI Junjie, TAN Aiping. Detection of infectious spleen and kidney necrosis virus (ISKNV) of mandarin fish (Siniperca chuatsi) by nested PCR[J]. South China Fisheries Science, 2009, 5(4): 69-72. DOI: 10.3969/j.issn.1673-2227.2009.04.013
    [9]JIANG Jufeng, ZHANG Dianchang, SU Tianfeng, XIONG Xiaofei, JIANG Shigui. Recombinant expression and antibody preparation of mud carp growth hormone[J]. South China Fisheries Science, 2008, 4(1): 36-40.
    [10]MU Xidong, BAI Junjie, YE Xing, WANG Xuejie, HU Yinchang. Effects of exogenous estradiol-17β on pituitary-thyroid axis of Carassius auratus[J]. South China Fisheries Science, 2007, 3(1): 31-36.

  • Cited by

    Periodical cited type(15)

    1. 周志希，唐汇娟，柯志新，刘甲星，周伟华. 基于形态学和高通量测序的春季南澳海域浮游植物群落特征及其与环境因子关系. 热带海洋学报. 2025(01): 53-65 .
    2. 柳原，柯志新，李开枝，谭烨辉，梁竣策，周伟华. 人类活动和沿岸流影响下的粤东近海浮游动物群落特征. 热带海洋学报. 2024(04): 98-111 .
    3. 薛宇，于浩林，周广军，赵伟，唐衍力. 渤海小竹山岛海洋牧场区营养盐分布特征及增养殖适宜性探究. 渔业科学进展. 2023(01): 1-12 .
    4. 黎素菊，洪捷娴，陈树鹏. 柘林湾养殖区氮、磷季节分布特征及富营养化评价. 江西水产科技. 2022(04): 45-47+51 .
    5. 匡翠萍，王丹，赵钒，刘会欣，朱磊. 金梦海湾及其邻近海域水环境因子时空分布特征. 同济大学学报(自然科学版). 2021(06): 880-890 .
    6. 任玉正，柯志新，谭烨辉，李开枝. 广东省南澳岛东部海域浮游动物群落结构及其影响因素. 热带海洋学报. 2020(02): 65-76 .
    7. 杨文超，黄道建，陈继鑫，陈晓燕，王宇珊. 大亚湾海域2009—2015年氮、磷营养盐时空分布及富营养化评价. 南方水产科学. 2020(02): 54-61 . 本站查看
    8. 陈丹婷，柯志新，谭烨辉，刘甲星. 汕头南澳—东山海域营养盐季节分布特征及其对浮游植物生长的潜在性限制. 生态科学. 2020(04): 41-50 .
    9. 马欢，秦传新，陈丕茂，林会洁，段丁毓. 柘林湾海洋牧场生态系统服务价值评估. 南方水产科学. 2019(01): 10-19 . 本站查看
    10. 王言丰，胡启伟，余景，陈丕茂，舒黎明. 粤东柘林湾海洋牧场渔业资源增殖效果评估. 南方水产科学. 2019(02): 12-19 . 本站查看
    11. 徐淑敏，齐占会，史荣君，刘永，韩婷婷，黄洪辉. 水产养殖对亚热带海湾氮磷营养盐时空分布的影响——以深澳湾为例. 南方水产科学. 2019(04): 29-38 . 本站查看
    12. 王文杰，陈丕茂，袁华荣，冯雪，张露，龙鑫玲，陈文静，李丹丹. 粤东柘林湾甲壳类群落结构季节变化分析. 南方水产科学. 2018(03): 29-39 . 本站查看
    13. 佟飞，秦传新，余景，陈丕茂. 粤东柘林湾溜牛人工鱼礁建设选址生态基础评价. 南方水产科学. 2016(06): 25-32 . 本站查看
    14. 舒黎明，陈丕茂，秦传新，黎小国，周艳波，冯雪，于杰，袁华荣，李国迎. 柘林湾-南澳岛潮间带冬夏两季大型底栖动物种类组成及优势种. 生态学杂志. 2016(02): 423-430 .
    15. 舒黎明，陈丕茂，黎小国，秦传新，于杰，周艳波，袁华荣. 柘林湾附近海域大型底栖动物物种多样性. 中国水产科学. 2015(03): 501-516 .

    Other cited types(5)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(20)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return