Turn off MathJax
Article Contents
Abstract
References
LIU Chenyuan, SHANG Yukun, MAO Huili, LI Saibo, WANG Qiankun, ZHANG Xingxing, ZHU Lixing, ZHANG Ziyi, SHEN Changchun, GUAN Jianyi. Isolation and identification of a pathogenic Nocardia seriolae strain XXLX2 from seabass and comparative genomic analysis[J]. South China Fisheries Science. DOI: 10.12131/20240254
Citation: LIU Chenyuan, SHANG Yukun, MAO Huili, LI Saibo, WANG Qiankun, ZHANG Xingxing, ZHU Lixing, ZHANG Ziyi, SHEN Changchun, GUAN Jianyi. Isolation and identification of a pathogenic Nocardia seriolae strain XXLX2 from seabass and comparative genomic analysis[J]. South China Fisheries Science. DOI: 10.12131/20240254
shu

Isolation and identification of a pathogenic Nocardia seriolae strain XXLX2 from seabass and comparative genomic analysis

  • School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China

More Information
  • Received Date: October 30, 2024
  • Revised Date: February 11, 2025
  • Accepted Date: February 23, 2025
  • Available Online: April 08, 2025
    Graphical Abstract
    • Abstract

  • We isolated and identified the pathogen causing disease in largemouth bass (Micropterus salmoides) from a farm in Xinxiang, and investigated its pathogenicity. We combined the annotation of drug-resistant genes to analyze drug sensitivity results, and compared its genome structure, virulence factors and closely related strains to search for the common immune protective antigen functional genes. By the analyses of colony morphology and physicochemical properties, and based on the identification of isolated bacteria through 16S rRNA gene sequence alignment, we conducted hemolysis test, artificial regression infection test, drug sensitivity test, and whole genome sequencing analysis, then identified the isolated bacteria XXLX2 which was identified as Nocardia seriolae, without hemolysis circle on blood agar plates. The median lethal dose (LD50) for largemouth bass was 1.49×105 CFU·mL−1 through regression infection tests, and the symptoms of infected bass were consistent with those of naturally diseased bass. The XXLX2 strain exhibited resistance to polymyxin B, erythromycin, and β-lactam antibiotics, and the results of drug resistance gene and drug sensitivity test were generally consistent. Through genome comparison analysis, the XXLX2 strain showed close phylogenetic relationship and good collinearity with three strains of N. seriolae from different sources, namely EM150506, NK201610020, and UTF1. There were certain differences in the comparison of virulence factors between the XXLX2 strain and the three strains of N. seriolae, but generally, they exhibited high conservation. Genomic annotation and comparative analysis of the XXLX2 strain provide basic data support for further exploration of the pathogenic mechanism of N. seriolae and research on genetically engineered vaccines.

    • FullText(HTML)
    • References (35)
  • [1]
    张媛, 张媛媛, 李振军, 等. 诺卡氏菌研究进展[J]. 中国人兽共患病报, 2012, 28(6): 628-634.
    [2]
    袁思平, 王国良, 金珊. 养殖鱼类致病诺卡氏菌研究进展[J]. 微生物学通报, 2006, 33(2): 137-141. doi: 10.3969/j.issn.0253-2654.2006.02.028
    [3]
    王文基, 陈建林, 侯素莹, 等. 鰤鱼诺卡氏菌感染乌斑杂交鳢的组织病理学研究[J]. 基因组学与应用生物学, 2019, 38(10): 4439-4446.
    [4]
    夏立群, 汪美, 赖杰彬, 等. 鰤鱼诺卡氏菌感染斑马鱼模型的建立与组织病理学研究[J]. 热带生物学报, 2016, 7(4): 409-416.
    [5]
    MAEKAWA S, YOSHIDA T, WANG P C, et al. Current knowledge of nocardiosis in teleost fish[J]. J Fish Dis, 2018, 41(3): 413-419. doi: 10.1111/jfd.12782
    [6]
    ZHOU Z Y, BAI S J, LU C W, et al. Nocardia seriolae mediates liver granulomatous chronic inflammation in Micropterus salmoides through pyroptosis[J]. J Fish Dis, 2023, 46(4): 321-332. doi: 10.1111/jfd.13746
    [7]
    WANG W J, CHEN J L, LIAO B S, et al. Identification and functional characterization of histone-like DNA-binding protein in Nocardia seriolae (NsHLP) involved in cell apoptosis[J]. J Fish Dis, 2019, 42(5): 657-666. doi: 10.1111/jfd.12962
    [8]
    HOU S Y, CHEN G Q, WANG W J, et al. Identification of a cell-wall peptidase (NlpC/P60) from Nocardia seriolae which induces a-poptosis in fathead minnow cells[J]. J Fish Dis, 2020, 43(5): 571-581. doi: 10.1111/jfd.13154
    [9]
    TENG J, ZHAO Y, MENG Q L, et al. Transcriptome analysis in the spleen of northern snakehead (Channa argus) challenged with Nocardia seriolae[J]. Genomics, 2022, 114(3): 110357. doi: 10.1016/j.ygeno.2022.110357
    [10]
    WANG G L, YUAN S P, JIN S. Nocardiosis in large yellow croaker, Larimichthys crocea (Richardson)[J]. J Fish Dis, 2005, 28(6): 339-345. doi: 10.1111/j.1365-2761.2005.00637.x
    [11]
    LEI X P, ZHAO R X, GENG Y, et al. Nocardia seriolae: a serious threat to the largemouth bass Micropterus salmoides industry in Southwest China[J]. Dis Aquat Organ, 2020, 142: 13-21. doi: 10.3354/dao03517
    [12]
    夏立群, 黄郁葱, 鲁义善. 卵形鲳鲹主要病害及其研究进展[J]. 安徽农学通报 (上半月刊), 2012, 18(23): 140-143,150.
    [13]
    KIM B S, PARK J W, KANG G S, et al. First report of Nocardia infection in cultured Japanese eel, Anguilla japonica[J]. J Fish Dis, 2018, 41(12): 1921-1927. doi: 10.1111/jfd.12882
    [14]
    HOLT J G, KRIEG N R, SNEATH P H A. Bergey's manual of determinative bacteriology[M]. 9th ed. Baltimore: Lippincott Williams & Wilkins, 1993: 175-225.
    [15]
    顾兵, 张政, 李玉萍, 等. 半数致死量及其计算方法概述[J]. 中国职业医学, 2009, 36(6): 507-508,511.
    [16]
    CHAN P P, LOWE T M. tRNAscan-SE: searching for tRNA genes in genomic sequences[J]. Methods Mol Biol, 2019, 1962: 1-14.
    [17]
    ALCOCK B P, RAPHENYA A R, LAU T T Y, et al. CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database[J]. Nucleic Acids Res, 2020, 48(D1): D517-D525.
    [18]
    LIU B, ZHENG D D, ZHOU S Y, et al. VFDB 2022: a general classification scheme for bacterial virulence factors[J]. Nucleic Acids Res, 2022, 50(D1): D912-D917. doi: 10.1093/nar/gkab1107
    [19]
    WANG F, WANG X G, LIU C, et al. Transparent tiger barb Puntius tetrazona, a fish model for in vivo analysis of nocardial infection[J]. Vet Microbiol, 2017, 211: 67-73. doi: 10.1016/j.vetmic.2017.10.003
    [20]
    吴迪, 阮泽超, 王跃斌, 等. 小黄鱼 (Larimichthys polyactis) 鰤鱼诺卡氏菌的分离及鉴定[J]. 海洋与湖沼, 2023, 54(4): 1182-1190.
    [21]
    张美超, 邓玉婷, 赵飞, 等. 一株鳢源鰤诺卡氏菌致病性与全基因组分析[J]. 微生物学通报, 2022, 49(6): 2193-2211.
    [22]
    吕丽丽, 梅飞, 曹守林, 等. 加州鲈源鰤鱼诺卡氏菌的分离鉴定及致病性[J]. 微生物学通报, 2021, 48(12): 4765-4775.
    [23]
    YASUIKE M, NISHIKI I, IWASAKI Y, et al. Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: the first reference genome sequence of the fish pathogenic Nocardia species[J]. PLoS One, 2017, 12(3): e0173198. doi: 10.1371/journal.pone.0173198
    [24]
    HAN H J, KWAK M J, HA S M, et al. Genomic characterization of Nocardia seriolae strains isolated from diseased fish[J]. Microbiologyopen, 2019, 8(3): e00656. doi: 10.1002/mbo3.656
    [25]
    朱志东, 吕莉, 邓剑壕, 等. 鱼类诺卡氏菌病的研究进展[J]. 水产养殖, 2018, 39(1): 48-52. doi: 10.3969/j.issn.1004-2091.2018.01.009
    [26]
    陈言峰, 范祖游, 王子涛, 等. 水产动物诺卡氏菌病防控技术的研究进展[J]. 安徽农业科学, 2015, 43(13): 170-172. doi: 10.3969/j.issn.0517-6611.2015.13.059
    [27]
    WENG T T, CHEN G Q, LI N, et al. Identifying the in vivo-induced antigenic genes is a strategy to develop DNA vaccine against Nocardia seriolae in hybrid snakehead (Channa maculata ♀ × Channa argus ♂)[J]. Fish Shellfish Immunol, 2024, 147: 109410. doi: 10.1016/j.fsi.2024.109410
    [28]
    CAO S L, CHANG J J, YUE X Z, et al. Potential virulence factors of Nocardia seriolae AHLQ20-01 based on whole-genome analysis and its pathogenicity to largemouth bass (Micropterus salmoides)[J]. J Fish Dis, 2023, 46(4): 333-345. doi: 10.1111/jfd.13747
    [29]
    GEBICKA L, KRYCH-MADEJ J. The role of catalases in the prevention/promotion of oxidative stress[J]. J Inorg Biochem, 2019, 197: 110699. doi: 10.1016/j.jinorgbio.2019.110699
    [30]
    HOU S Y, WANG W J, CHEN G Q, et al. Identification of a secreted superoxide dismutase (SOD) from Nocardia seriolae which induces apoptosis in fathead minnow (FHM) cells[J]. J Fish Dis, 2021, 44(1): 63-72. doi: 10.1111/jfd.13268
    [31]
    罗愿, 邓玉婷, 赵飞, 等. 9株鱼源鰤诺卡氏菌生物学特征和致病性比较[J]. 微生物学通报, 2021, 48(8): 2733-2749.
    [32]
    KHAN A, SARKAR D. Nitrate reduction pathways in mycobacteria and their implications during latency[J]. Microbiology, 2012, 158(Pt 2): 301-307.
    [33]
    谢龙祥. 结核分枝杆菌表观遗传与耐药新机理研究[D]. 重庆: 西南大学, 2017: 109.
    [34]
    PANDEY R, RODRIGUEZ G M. IdeR is required for iron homeostasis and virulence in Mycobacterium tuberculosis[J]. Mol Microbiol, 2014, 91(1): 98-109. doi: 10.1111/mmi.12441
    [35]
    GONZALEZ-CARRILLO C, MILLAN-SAUCEDA C, LOZANO-GARZA H G, et al. Geno-mic changes associated with the loss of Nocardia brasiliensis virulence in mice after 200 in vitro passages[J]. Infect Immun, 2016, 84(9): 2595-2606. doi: 10.1128/IAI.00329-16
    • Related Articles

Catalog

    Get Citation
    PDF
    XML
    Article views (38) PDF downloads (0) Cited by()
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return