| Citation: |
ZHONG Zhanyou, DENG Hong, KOU Chunni, CHEN Weitao, WU Zhi, LI Yuefei, XIA Yuguo, LI Huifeng, LI Jie, ZHU Shuli. Research on fish diversity in Xijiang Rare Fish Provincial Nature Reserve based on environmental DNA technology[J]. South China Fisheries Science, 2025, 21(2): 47-58. DOI: 10.12131/20240173
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To better protect the fish resources in the Xijiang Rare Fish Provincial Nature Reserve, we used the environmental DNA (eDNA) technology to analyze the fish species composition and fish diversity at different cross sections of 18 sampling stations on the left, middle and right banks of the river section. Two molecular markers, i.e. COI and 12S rRNA, were used in this study. The results show that the two genes of 12S rRNA and COI were detected in 53 fish species, belonging to 48 genera, 12 families and 4 orders. Forty-seven and nineteen fish species were examined based on 12S rRNA and COI, respectively. In addition, 13 shared fish species were discovered by using the two gene markers. Alpha diversity analysis based on sequence abundance shows that the two markers unanimously supported the highest number of fish species and the highest richness of Chao 1 index on the left bank of the river section, indicating that the left bank appears to have higher fish diversity than the middle and right banks. Beta diversity analysis based on 12S rRNA shows obvious differences in the fish composition on the left, middle and right banks. The research provides basic data and technical support for the protection and dynamic monitoring of fish biodiversity in this nature reserve.
| [1] |
李淼, 许友伟, 孙铭帅, 等. 气候变化对海洋鱼类群落结构的影响研究进展[J]. 海洋科学, 2022, 46(7): 120-129.
|
| [2] |
HUANG M R, DING L Y, WANG J, et al. The impacts of climate change on fish growth: a summary of conducted studies and current knowledge[J]. Ecol Indic, 2020, 121: 106976.
|
| [3] |
MCKENZIE D J, GEFFROY B, FARRELL A P. Effects of global warming on fishes and fisheries[J]. J Fish Biol, 2021, 98(6): 1489-1492.
|
| [4] |
中国野生动物保护协会水生野生动物保护分会. 中国水生野生动物保护蓝皮书[M]. 北京: 海洋出版社, 2021: 3-6.
|
| [5] |
赵明, 赵梦迪, 马春艳, 等. 环境DNA在水域生态中的研究进展[J]. 中国水产科学, 2018, 25(4): 714-720.
|
| [6] |
舒璐, 林佳艳, 徐源, 等. 基于环境DNA宏条形码的洱海鱼类多样性研究[J]. 水生生物学报, 2020, 44(5): 1080-1086. doi: 10.7541/2020.125
|
| [7] |
李苗, 陈小勇. 环境DNA技术在鱼类生态学中的应用研究进展[J]. 生态学报, 2023, 43(17): 6951-6967.
|
| [8] |
YAO M, ZHANG S, LU Q, et al. Fishing for fish environmental DNA: ecological applications, methodological considerations, surveying designs, and ways forward[J]. Mol Ecol, 2022, 31(2022): 5132-5164.
|
| [9] |
DEINER K, WALSER J C, MÄCHLER E, et al. Choice of capture and extraction methods affect detection of freshwater biodiversity from environmental DNA[J]. Biol Conserv, 2015, 183(1): 53-63.
|
| [10] |
RUPPERT K M, KLINE R J, RAHMAN M S. Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: a systematic review in methods, monitoring, and applications of global eDNA[J]. Global Ecol Conserv, 2019, 17: e00547. doi: 10.1016/j.gecco.2019.e00547
|
| [11] |
SHU L, LUDWIG A, PENG Z G. Standards for methods utilizing environmental DNA for detection of fish species[J]. Genes-Basel, 2020, 11(3): 296. doi: 10.3390/genes11030296
|
| [12] |
秦传新, 左涛, 于刚, 等. 环境DNA在水生生态系统生物量评估中的研究进展[J]. 南方水产科学, 2020, 16(5): 123-128. doi: 10.12131/20190256
|
| [13] |
金珂, 张丽娟, 张伟, 等. 基于环境DNA宏条形码的太湖流域底栖动物监测与生态健康评价[J]. 中国环境监测, 2022, 38(1): 175-188.
|
| [14] |
OGRAM A, SAYLER G S, BARKAY T. The extraction and purification of microbial DNA from sediments[J]. J Microbiol Methods, 1987, 7(3): 57-66.
|
| [15] |
FICETOLA G F, MIAUD C, POMPANON F, et al. Species detection using environmental DNA from water samples[J]. Biol Lett, 2008, 4(4): 423-425. doi: 10.1098/rsbl.2008.0118
|
| [16] |
SIGSGAARD E E, NIELSEN I B, CARL H, et al. Seawater environmental DNA reflects seasonality of a coastal fish community[J]. Mar Biol, 2017, 164(6): 128. doi: 10.1007/s00227-017-3147-4
|
| [17] |
程如丽, 罗杨, 张玉凤, 等. 基于环境DNA技术的乌江干流梯级水电库区的鱼类多样性[J]. 水产学报, 2025, 49(3): 039310.
|
| [18] |
李萌, 尉婷婷, 史博洋, 等. 环境DNA技术在淡水底栖大型无脊椎动物多样性监测中的应用[J]. 生物多样性, 2019, 27(5): 480-490. doi: 10.17520/biods.2018227
|
| [19] |
周春花, 王蓉蓉, 王生, 等. 基于环境DNA宏条形码技术的赣江下游(南昌段)鱼类多样性[J]. 湖泊科学, 2023, 35(4): 1423-1440. doi: 10.18307/2023.0435
|
| [20] |
廖敏. 雅砻江锦屏一级库区鱼类夏秋季分布格局与环境因子关系初步研究[D]. 雅安: 四川农业大学, 2022: 76.
|
| [21] |
李捷, 李新辉, 谭细畅, 等. 广东肇庆西江珍稀鱼类省级自然保护区鱼类多样性[J]. 湖泊科学, 2009, 21(4): 556-562. doi: 10.3321/j.issn:1003-5427.2009.04.015
|
| [22] |
谭细畅, 李新辉, 林建志, 等. 基于水声学探测的两个广东鲂产卵群体繁殖生态的差异性[J]. 生态学报, 2009, 29(4): 1756-1762. doi: 10.3321/j.issn:1000-0933.2009.04.016
|
| [23] |
刘亚秋, 李新辉, 李跃飞, 等. 西江广东鲂 (Megalobrama terminalis) 繁殖生物学及繁殖策略[J]. 湖泊科学, 2021, 33(1): 232-241. doi: 10.18307/2021.0117
|
| [24] |
李跃飞, 李策, 朱书礼, 等. 基于单位补充量模型的西江广东鲂种群资源利用现状评价[J]. 水生生物学报, 2018, 42(5): 975-983.
|
| [25] |
ZHANG S, ZHAO J D, YAO M. A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish[J]. Methods Ecol Evol, 2020, 11(12): 1609-1625. doi: 10.1111/2041-210X.13485
|
| [26] |
OWEN S W, CREU P, MAGDALENA G, et al. DNA metabarcoding of littoral hard-bottom communities: high diversity and database gaps revealed by two molecular markers[J]. PeerJ, 2018, 6: e4705. doi: 10.7717/peerj.4705
|
| [27] |
郑慈英. 珠江鱼类志[M]. 北京: 科学出版社, 1989: 77-367.
|
| [28] |
张春光, 赵亚辉. 中国内陆鱼类物种与分布[M]. 北京: 科学出版社, 2016: 210-213.
|
| [29] |
CHAO A. Nonparametric estimation of the number of classes in a population[J]. Scand J Stat, 1984, 11(4): 265-270.
|
| [30] |
SHANNON C E. A mathematical theory of communication[J]. Bell Sys Tech J, 1948, 27(3): 379-423. doi: 10.1002/j.1538-7305.1948.tb01338.x
|
| [31] |
SIMPSON E H. Measurement of diversity[J]. Nature, 1949, 163(4148): 688. doi: 10.1038/163688a0
|
| [32] |
DIXON P. VEGAN, a package of R functions for community ecology[J]. J Veg Sci, 2003, 14(6): 927-930. doi: 10.1111/j.1654-1103.2003.tb02228.x
|
| [33] |
CHEN Y S, QU X, XIONG F, et al. Challenges to saving China's freshwater biodiversity: fishery exploitation and landscape pressures[J]. Ambio, 2020, 49: 926-938. doi: 10.1007/s13280-019-01246-2
|
| [34] |
REID A J, CARLSON A K, CREED I F, et al. Emerging threats and persistent conservation challenges for freshwater biodiversity[J]. Biol Rev, 2019, 94(3): 849-873. doi: 10.1111/brv.12480
|
| [35] |
COULTHARD S, JOHNSON D, MCGREGOR J A. Poverty, sustainability and human wellbeing: a social wellbeing approach to the global fisheries crisis[J]. Global Environ Change, 2011, 21(2): 453-463. doi: 10.1016/j.gloenvcha.2011.01.003
|
| [36] |
DUDGEON D. Multiple threats imperil freshwater biodiversity in the Anthropocene[J]. Curr Biol, 2019, 29(19): R960-R967. doi: 10.1016/j.cub.2019.08.002
|
| [37] |
张改, 武智, 朱书礼, 等. 清远水利枢纽建设对库区鱼类群落结构影响[J]. 生态科学, 2021, 40(2): 175-185.
|
| [38] |
帅方敏, 李新辉, 刘乾甫, 等. 珠江水系鱼类群落多样性空间分布格局[J]. 生态学报, 2017, 37(9): 3182-3192.
|
| [39] |
吴倩, 李潮, 高天扬, 等. 流溪河保护区鱼类群落结构及其时空变动[J]. 水产科学, 2020, 39(2): 234-244.
|
| [40] |
顾党恩, 牟希东, 罗渡, 等. 广东省主要水系罗非鱼的建群状况[J]. 生物安全学报, 2012, 21(4): 277-282.
|
| [41] |
李德越, 李荣辉, 吴志强, 等. 广西南宁大王滩水库鱼类物种组成及多样性分析[J]. 南方水产科学, 2018, 14(2): 110-117. doi: 10.3969/j.issn.2095-0780.2018.02.015
|
| [42] |
吴映明, 唐以杰, 黄更生. 广东饶平海山河口区红树林潮沟鱼类时空生态位[J]. 中山大学学报 (自然科学版) (中英文), 2024, 63(3): 71-79.
|
| [43] |
巴家文, 陈大庆. 三峡库区的入侵鱼类及库区蓄水对外来鱼类入侵的影响初探[J]. 湖泊科学, 2012, 24(2): 185-189. doi: 10.3969/j.issn.1003-5427.2012.02.003
|
| [44] |
朱书礼, 陈蔚涛, 武智, 等. 基于环境DNA技术的珠江中下游鱼类多样性初步研究[J]. 南方水产科学, 2024, 20(1): 120-129. doi: 10.12131/20230111
|
| [45] |
BYLEMANS J, GLEESON D M, LINTERMANS M, et al. Monitoring riverine fish communities through eDNA metabarcoding: determining optimal sampling strategies along an altitudinal and biodiversity gradient[J]. Metabarc Metagenome, 2018, 2: 1-12.
|
| [46] |
OKA S, DOI H, MIYAMOTO K, et al. Environmental DNA metabarcoding for biodiversity monitoring of a highly diverse tropical fish community in a coral reef lagoon: estimation of species richness and detection of habitat segregation[J]. Environ DNA, 2021, 3(1): 55-69.
|
| [47] |
陈治, 蔡杏伟, 张清凤, 等. 海南岛淡水鱼类环境DNA宏条形码参考数据库的初步构建及比较分析[J]. 南方水产科学, 2022, 18(3): 1-12. doi: 10.12131/20210339
|
| [48] |
COLLINS R A, BAKKER J, WANGENSTEEN O S, et al. Non-specific amplification compromises environmental DNA metabarcoding with COI[J]. Methods Ecol Evol, 2019, 10(11): 1985-2001. doi: 10.1111/2041-210X.13276
|
| [49] |
刘军, 赵良杰, 凡迎春, 等. 鱼类环境DNA研究中通用引物的筛选验证[J]. 淡水渔业, 2016, 46(1): 9-17. doi: 10.3969/j.issn.1000-6907.2016.01.002
|
| [50] |
蒋佩文, 李敏, 张帅, 等. 基于环境DNA宏条码和底拖网的珠江河口鱼类多样性[J]. 水生生物学报, 2022, 46(11): 1701-1711. doi: 10.7541/2022.2021.0265
|
| [51] |
DJURHUUS A, CLOSEK C J, KELLY R P, et al. Environmental DNA reveals seasonal shifts and potential interactions in a marine community[J]. Nature Commun, 2020, 11(1): 254. doi: 10.1038/s41467-019-14105-1
|
| [52] |
冯启新, 王金潮, 尤炳赞, 等. 广东鲂产卵场调查报告[J]. 淡水渔业, 1986(6): 1-5.
|
| [53] |
SANSOM B J, SASSOUBRE L M. Environmental DNA (eDNA) shedding and decay rates to model freshwater mussel eDNA transport in a river[J]. Environ Sci Technol, 2017, 51(24): 14244-14253. doi: 10.1021/acs.est.7b05199
|
| [54] |
PILLIOD D S, GOLDBERG C S, ARKLE R S, et al. Factors influencing detection of eDNA from a stream-dwelling amphibian[J]. Mol Ecol Resour, 2014, 14(1): 109-116. doi: 10.1111/1755-0998.12159
|
| [55] |
DEJEAN T, VALENTINI A, DUPARC A, et al. Persistence of environmental DNA in freshwater ecosystems[J]. PLoS One, 2011, 6(8): e23398. doi: 10.1371/journal.pone.0023398
|
| [56] |
STEWART K A. Understanding the effects of biotic and abiotic factors on sources of aquatic environmental DNA[J]. Biodivers Conserv, 2019, 28(5): 983-1001. doi: 10.1007/s10531-019-01709-8
|
| [57] |
BARNES M A, CHADDERTON W L, JERDE C L, et al. Environmental conditions influence eDNA particle size distribution in aquatic systems[J]. Environ DNA, 2021, 3(3): 643-653. doi: 10.1002/edn3.160
|
| [58] |
SAITO T, DOI H. Effect of salinity and water dilution on environmental DNA degradation in freshwater environments[J]. bioRxiv, 2021, 5(24): 445344.
|
| [59] |
STRICKLER K, FREMIER A K, GOLDBERG C S. Quantifying effects of UV-B, temperature, and pH on eDNA degradation in aquatic microcosms[J]. Biol Conserv, 2015, 183: 85-92. doi: 10.1016/j.biocon.2014.11.038
|
| [60] |
ELBRECHT V, VAMOS E E, MEISSNER K, et al. Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring[J]. Methods Ecol Evol, 2017, 8(10): 1265-1275. doi: 10.1111/2041-210X.12789
|
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