Volume 21 Issue 1
Feb.  2025
Turn off MathJax
Article Contents
Abstract
References
Related Articles
SHEN Chuyan, ZHANG Zhe, CHEN Haigang, ZHANG Linbao, WANG Xuefeng. Effects of LH crude oil and No.0 diesel oil emulsion on hepatopancreatic antioxidant enzyme activity and related functional gene expression in Litopenaeus vannamei[J]. South China Fisheries Science, 2025, 21(1): 118-130. DOI: 10.12131/20240153
Citation: SHEN Chuyan, ZHANG Zhe, CHEN Haigang, ZHANG Linbao, WANG Xuefeng. Effects of LH crude oil and No.0 diesel oil emulsion on hepatopancreatic antioxidant enzyme activity and related functional gene expression in Litopenaeus vannamei[J]. South China Fisheries Science, 2025, 21(1): 118-130. DOI: 10.12131/20240153
shu

Effects of LH crude oil and No.0 diesel oil emulsion on hepatopancreatic antioxidant enzyme activity and related functional gene expression in Litopenaeus vannamei

  • 1.

    College of Fisheries, Guangdong Ocean University, Zhanjiang 554088, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Marine Ranching, Ministry of Agriculture and Rural Affairs/Scientific Observing and Experimental Station of South China Sea Fishery Resources and Environment, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment/Scientific Observation and Research Field Station of Pearl River Estuary Ecosystem, Guangzhou 510300, China

More Information
  • Received Date: July 03, 2024
  • Revised Date: September 20, 2024
  • Accepted Date: October 22, 2024
  • Available Online: November 18, 2024
    Graphical Abstract
    • Abstract

  • Marine oil spills cause multiple threats to the growth and development of marine organisms in coastal areas. To investigate the toxic effects of LH crude oil and No.0 diesel emulsion on the hepatopancreas of Litopenaeus vannamei, we divided LH crude oil and No.0 diesel into a dispersant control group (Group D, 0 μg·L−1), a low concentration group (Group A, 25 μg·L−1), a medium concentration group (Group B, 50 μg·L−1), and a high concentration group (Group C, 250 μg·L−1). Then we measured the changes of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione peroxidase (GPx), glutathione S-transferase (GST) and glutathione (GSH), as well as the expression of related functional genes SOD, CAT, GPx, lysozyme (LZM), apoptosis factor (caspase2), peroxiredoxin (Prx), elongation factor (EF) and prophenoloxidase (proPO) on the 15th day. The results show that: 1) Both LH crude oil and No.0 diesel oil emulsions stress caused changes in the antioxidant system and related functional genes in the hepatopancreas of L. vannamei, and the effects of No.0 diesel oil were more significant than that of LH crude oil. 2) The integrated biomarker response (IBR) index showed a dose-effect and time-effect pattern, rising with increasing stress concentration and prolonged time. 3) The results of principal component analysis (PCA) show that LH crude oil and No.0 diesel oil significantly affected the antioxidant enzyme activities and the relative expression of caspase2. However, there was a difference in the biomarkers sensitivity to crude oil and diesel oil. The study reveals that LH crude oil and No.0 diesel oil emulsion significantly affect the hepatopancreatic antioxidant enzyme activities and the expression of related functional genes in L. vannamei, affecting its normal physiological functions. The results provides fundamental data support for assessing the toxic effects of petroleum pollution on L. vannamei.

    • FullText(HTML)
    • References (53)
  • [1]
    祁璇, 朱晓婧, 高倩倩, 等. 原油与柴油泄露影响海洋飞沫气溶胶中重金属与多环芳烃分布的实验室模拟研究[J]. 复旦学报 (自然科学版), 2024, 63(1): 122-133.
    [2]
    LINDÉN O. Biological effects of oil on early development of the Baltic herring Clupea harengus membras[J]. Mar Biol, 1978, 45: 273-282. doi: 10.1007/BF00390611
    [3]
    李井懿, 童一帆, 程千卉, 等. 海水中原油对双齿围沙蚕 (Perinereis aibuhitensis) 的急性毒性效应及其体内抗氧化酶活性的影响[J]. 环境科学研究, 2018, 31(11): 1972-1978.
    [4]
    张喆, 陈海刚, 温为庚, 等. 0# 柴油和流花原油对斑节对虾 (Penaeus monodon) 幼体的急性毒性效应[J]. 生态毒理学报, 2017, 12(6): 242-251. doi: 10.7524/AJE.1673-5897.20160620001
    [5]
    李佩潼. 分散剂作用下原油对海洋藻类的急性毒性研究[D]. 青岛: 青岛理工大学, 2016: 36-47.
    [6]
    肖雅元, 李纯厚, 徐姗楠, 等. 水溶性石油烃组分对翡翠贻贝的毒性效应研究[J]. 农业环境科学学报, 2012, 31(3): 598-606.
    [7]
    HAGGER J A, JONES M B, LEONARD D P, et al. Biomarkers and integrated environmental risk assessment: are there more questions than answers?[J]. Integr Environ Assess Manage, 2006, 2(4): 312-329. doi: 10.1002/ieam.5630020403
    [8]
    蒋凤华, 高伟, 赵美丽, 等. 原油污染对栉孔扇贝抗氧化酶活性的影响[J]. 海洋科学, 2012, 36(7): 28-33.
    [9]
    XIA B, CHEN B J, SUN X M, et al. Toxicological effects of crude oil: integrated biomarker responses in the hepatopancreas of clam Ruditapes philippinarum[J]. Asian J Chem, 2014, 26(12): 3631-3638.
    [10]
    BELIAEFF B, BURGEOT T. Integrated biomarker response: a useful tool for ecological risk assessment[J]. Environ Toxicol Chem, 2002, 21(6): 1316-1322.
    [11]
    WANG Y J, CHEN C Z, LI P, et al. Chronic toxic effects of waterborne mercury on silver carp (Hypophthalmichthys molitrix) larvae[J]. Water, 2022, 14(11): 1774. doi: 10.3390/w14111774
    [12]
    JIANG M, LI L, LI Y R, et al. Oxidative stress in shellfish Sinonovacula constricta exposed to the water accommodated fraction of zero sulfur diesel oil and pinghu crude oil[J]. Arch Environ Con Tox, 2017, 73(2): 294-300. doi: 10.1007/s00244-017-0391-z
    [13]
    农业农村部渔业渔政管理局, 全国水产技术推广总站, 中国水产学会. 2024中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2024: 22-34.
    [14]
    刘丽诗, 陈义烘. 凡纳滨对虾环境胁迫响应信号通路研究进展[J]. 广东畜牧兽医科技, 2024, 49(1): 1-10.
    [15]
    黄南建, 陈海刚, 张林宝, 等. 南海LH原油和0# 柴油对3种海水增养殖生物的急性毒性研究[J]. 南方水产科学, 2015, 11(2): 72-78. doi: 10.3969/j.issn.2095-0780.2015.02.010
    [16]
    WANG P H, WAN D H, CHEN Y G, et al. Characterization of four novel caspases from Litopenaeus vannamei (Lvcaspase2-5) and their role in WSSV infection through dsRNA-mediated gene silencing[J]. PLoS One, 2013, 8(12): e80418. doi: 10.1371/journal.pone.0080418
    [17]
    LIU X L, XI Q Y, YANG L, et al. The effect of dietary Panax ginseng polysaccharide extract on the immune responses in white shrimp, Litopenaeus vannamei[J]. Fish Shellfish Immunol, 2011, 30(2): 495-500. doi: 10.1016/j.fsi.2010.11.018
    [18]
    WANG Y C, CHANG P S, CHEN H Y. Tissue expressions of nine genes important to immune defence of the Pacific white shrimp Litopenaeus vannamei[J]. Fish Shellfish Immunol, 2007, 23(6): 1161-1177.
    [19]
    LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method[J]. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262
    [20]
    SANCHEZ W, BURGEOT T, PORCHER J M. A novel "Integrated Biomarker Response" calculation based on reference deviation concept[J]. Environ Sci Pollut R, 2013, 20(5): 2721-2725. doi: 10.1007/s11356-012-1359-1
    [21]
    BRANDTS I, TELES M, GONÇALVES A P, et al. Effects of nanoplastics on Mytilus galloprovincialis after individual and combined exposure with carbamazepine[J]. Sci Total Environ, 2018, 643: 775-784.
    [22]
    李豫, 黄建盛, 陈有铭, 等. 低温胁迫对军曹鱼幼鱼血清生化指标、肝脏抗氧化酶活性及凋亡相关基因表达量的影响[J]. 广东海洋大学学报, 2022, 42(5): 18-26. doi: 10.3969/j.issn.1673-9159.2022.05.003
    [23]
    RUPPERT E E, FOX R S, BARNES R D. Invertebrate zoology: a functional evolutionary approach[M]. 7th ed. Belmont (Calif.): Brooks/Cole Thomson learning, 2004: 662-664.
    [24]
    李磊, 刘金殿, 黄士林, 等. 基于综合生物标志物响应评价苯并芘对脊尾白虾 (Exopalaemon carinicauda) 的毒性效应[J]. 海洋与湖沼, 2018, 49(2): 444-451.
    [25]
    王琪, 杨超超, 明艳, 等. 菲胁迫对泥鳅肝脏氧化毒性及可逆性研究[J]. 延安大学学报 (自然科学版), 2023, 42(4): 61-68.
    [26]
    杨涛, 陈海刚, 蔡文贵, 等. 翡翠贻贝内脏团抗氧化防御系统对菲胁迫的生物响应[J]. 应用与环境生物学报, 2011, 17(4): 504-508.
    [27]
    董冉, 蒋玫, 李磊, 等. 0# 柴油水溶性成分对黑鲷肝脏、鳃、肌肉组织中抗氧化酶活性的影响[J]. 海洋渔业, 2016, 38(2): 190-197. doi: 10.3969/j.issn.1004-2490.2016.02.010
    [28]
    姜招峰, 杨翰仪. 氧自由基对CAT、SOD和GPX的氧化修饰研究[J]. 北京联合大学学报 (自然科学版), 2003(3): 12-17.
    [29]
    王桂燕, 李锋, 史济月, 等. 四氯乙烯胁迫对草鱼抗氧化酶活性的影响及其机理[J]. 中国环境科学, 2014, 34(6): 1579-1585.
    [30]
    李传慧. 生物标志物法研究石油胁迫对半滑舌鳎的毒性效应[D]. 青岛: 中国海洋大学, 2012: 34-38.
    [31]
    李传慧, 陈碧鹃, 崔毅, 等. 胜利原油对半滑舌鳎幼鱼肝脏过氧化物酶、溶菌酶和鳃丝Na+-K+-ATPase活力的影响[J]. 海洋环境科学, 2011, 30(5): 681-685. doi: 10.3969/j.issn.1007-6336.2011.05.017
    [32]
    张先勇, 刁晓平, 王轶, 等. 苯并[a]芘对马氏珠母贝肝组织抗氧化酶活性的影响[J]. 生态毒理学报, 2011, 6(2): 171-175.
    [33]
    任加云, 潘鲁青, 苗晶晶. 苯并(a)芘和苯并(k)荧蒽混合物对栉孔扇贝毒理学指标的影响[J]. 环境科学学报, 2006(7): 1180-1186. doi: 10.3321/j.issn:0253-2468.2006.07.023
    [34]
    董泽元. 谷胱甘肽过氧化物酶模拟物的底物识别与催化机理研究[D]. 长春: 吉林大学, 2006: 9-10.
    [35]
    HOU J, LI L, XUE T, et al. Hepatic positive and negative antioxidant responses in zebrafish after intraperitoneal administration of toxic microcystin-LR[J]. Chemosphere, 2015, 120: 729-736.
    [36]
    邢镇. 高温胁迫下长牡蛎 (Crassostrea gigas) 氧化应激响应和评价研究[D]. 大连: 大连海洋大学, 2024: 4-6.
    [37]
    贾晓平, 林钦, 蔡文贵, 等. 原油和燃油对南海重要海水增养殖生物的急性毒性试验[J]. 水产学报, 2000, 24(1): 32-36.
    [38]
    KIM J H, RHEE J S, LEE J S, et al. Effect of cadmium exposure on expression of antioxidant gene transcripts in the river pufferfish, Takifugu obscurus (Tetraodontiformes)[J]. Comp Biochem Physiol C, 2010, 152(4): 473-479.
    [39]
    冉凤霞, 金文杰, 端智卓玛, 等. Zn2+ 胁迫对花斑裸鲤抗氧化关键基因表达和抗氧化酶活性的影响[J]. 大连海洋大学学报, 2021, 36(3): 406-413.
    [40]
    JIAO W Y, HAN Q, XU Y M, et al. Impaired immune function and structural integrity in the gills of common carp (Cyprinus carpio L.) caused by chlorpyrifos exposure: through oxidative stress and apoptosis[J]. Fish Shellfish Immunol, 2019, 86: 239-245.
    [41]
    YANG J, GONG Y F, CAI J Z, et al. Chlorpyrifos induces apoptosis and autophagy in common carp lymphocytes by influencing the TCR γ-dependent PI3K/AKT/JNK pathway[J]. Fish Shellfish Immunol, 2020, 99: 587-593. doi: 10.1016/j.fsi.2020.02.039
    [42]
    朱含开, 吴兆毅, 赵庆顺, 等. 五氯酚暴露诱导斑马鱼胚胎细胞凋亡相关基因的变化及caspase-2基因克隆和系统进化分析[J]. 生态毒理学报, 2008(4): 356-362.
    [43]
    CHEN E, PROESTOU G, BOURBEAU D, et al. Rapid up-regulation of peptide elongation factor EF-1α protein levels is an immediate early event during oxidative stress-induced apoptosis[J]. Exp Cell Res, 2000, 259(1): 140-148. doi: 10.1006/excr.2000.4952
    [44]
    TAHIR A, SECOMBES C J. The effects of diesel oil-based drilling mud extracts on immune responses of rainbow trout[J]. Arch Environ Con Tox, 1995, 29(1): 27-32. doi: 10.1007/BF00213083
    [45]
    李明帅. 盐碱胁迫对中华绒螯蟹生理功能的影响[D]. 哈尔滨: 东北农业大学, 2022: 16-31.
    [46]
    ZHUANG Y Y, YANG F F, XU D H, et al. Spliced leader-based analyses reveal the effects of polycyclic aromatic hydrocarbons on gene expression in the copepod Pseudodiaptomus poplesia[J]. Aquat Toxicol, 2017, 183: 114-126. doi: 10.1016/j.aquatox.2016.12.014
    [47]
    FRANCESCO R, MARIA E G. Oxidative pathways of chemical toxicity and oxidative stress biomarkers in marine organisms[J]. Mar Environ Res, 2014, 93: 106-117.
    [48]
    凌晨, 张美东, 沙航, 等. 低氧胁迫对鲢抗氧化酶活性及SODs基因表达的影响[J]. 淡水渔业, 2021, 51(3): 53-59. doi: 10.3969/j.issn.1000-6907.2021.03.008
    [49]
    XU K H, ZHANG Y D, HUANG Y M, et al. Toxicological effects of microplastics and phenanthrene to zebrafish (Danio rerio)[J]. Sci Total Environ, 2021, 757: 143730. doi: 10.1016/j.scitotenv.2020.143730
    [50]
    LU Y P, ZHANG X X, ZHENG P H, et al. Effects of microcystin-LR on behavior, histopathology, oxidative stress, non-specific immunity and gene expression of red claw crayfish (Cherax quadricarinatus)[J]. Aquac Rep, 2023, 33: 101805. doi: 10.1016/j.aqrep.2023.101805
    [51]
    郭子好, 杨志刚, 刘志伟, 等. 中华绒螯蟹 (Eriocheir sinensis) 延伸因子EF-2基因全长cDNA克隆及表达[J]. 海洋与湖沼, 2012, 43(6): 1239-1246. doi: 10.11693/hyhz201206030030
    [52]
    VRINDA S, ABDULAZIZ A, ABHILASH K S, et al. Neuroendocrine and immunotoxicity of polyaromatic hydrocarbon, chrysene in crustacean post larvae[J]. Ecotoxicology, 2019, 28(8): 964-972. doi: 10.1007/s10646-019-02094-2
    [53]
    TU H T, SILVESTRE F, WANG N, et al. A multi-biomarker approach to assess the impact of farming systems on black tiger shrimp (Penaeus monodon)[J]. Chemosphere, 2010, 81(10): 1204-1211.
    • Related Articles

  • Related Articles

    [1]ZHU Shuli, CHEN Weitao, WU Zhi, XIA Yuguo, YANG Jiping, LI Yuefei, LI Jie. Preliminary investigation of fish diversity in middle and lower reaches of Pearl River based on environmental DNA technology[J]. South China Fisheries Science, 2024, 20(1): 120-129. DOI: 10.12131/20230111
    [2]XIE Yufang, WU Peng, LIU Yong, XIAO Yayuan, TANG Guanglong, WANG Teng, LIN Lin, LI Chunhou. Study on habitat suitability of Coilia mystus in Pearl River Estuary, China[J]. South China Fisheries Science, 2023, 19(1): 22-29. DOI: 10.12131/20220029
    [3]LI Hongting, ZHANG Shuai, ZOU Keshu, CHEN Zuozhi, CHEN Xiaolei, JIANG Peiwen, CAO Yiting, LI Min. Establishment and optimization of environmental DNA extraction method from water of Pearl River Estuary[J]. South China Fisheries Science, 2022, 18(3): 30-37. DOI: 10.12131/20210304
    [4]CHEN Zhi, CAI Xingwei, ZHANG Qingfeng, LI Gaojun, MA Chunlai, SHEN Zhixin. Preliminary construction and comparative analysis of environmental DNA metabarcoding reference database of freshwater fishes in Hainan Island[J]. South China Fisheries Science, 2022, 18(3): 1-12. DOI: 10.12131/20210339
    [5]XIONG Pengli, CHEN Zuozhi, HOU Gang, ZHANG Shuai, QIU Yongsong, FAN Jiangtao, XU Shannan. Decadal change in biological traits of Collichthys lucidus in Pearl River Estuary[J]. South China Fisheries Science, 2021, 17(6): 31-38. DOI: 10.12131/20210072
    [6]YAN Yali, ZHANG Nan, GUO Huayang, GUO Liang, ZHU Kecheng, LIU Baosuo, ZHANG Dianchang. Species identification and phylogenetic relationship in Siganidae based on DNA barcoding[J]. South China Fisheries Science, 2019, 15(1): 100-105. DOI: 10.12131/20180083
    [7]SHUAI Fangmin, LI Zhiquan, LIU Guowen, LI Xinhui, LI Yuefei, YANG Jiping, LI Jie. Resource status of Japanese eel (Anguilla japonica) in the Pearl River Estuary[J]. South China Fisheries Science, 2015, 11(2): 85-89. DOI: 10.3969/j.issn.2095-0780.2015.02.012
    [8]GAO Yuan, LAI Zini, WANG Chao, PANG Shixun, WEI Taili, XIE Wenping, YANG Wanling. Community characteristics of zooplankton in Pearl River Estuary in summer of 2006[J]. South China Fisheries Science, 2008, 4(1): 10-15.
    [9]WANG Di, LIN Zhaojin. Spatial and temporal variations of fish community structure in the Pearl River Estuary waters[J]. South China Fisheries Science, 2006, 2(4): 37-45.
    [10]YANG Lin, ZHANG Xufeng, ZHANG Peng, TAN Yongguang. Composition of by-catch of shrimping beam trawl in the Pearl River Estuary, China[J]. South China Fisheries Science, 2005, 1(1): 27-34.

  • Cited by

    Periodical cited type(11)

    1. 郭瑶杰，万武波，王海山，叶乐，陈治. 3组常用鱼类eDNA宏条形码通用引物对三亚水环境样品的物种检出效果比较. 南方水产科学. 2025(01): 66-76 . 本站查看
    2. 薛向平，孙朝徽，刘霞，惠筠，李学军，李秀启，司飞. 基于COI基因的黄河河南-山东段鲤群体遗传结构分析. 淡水渔业. 2025(02): 43-52 .
    3. 朱书礼，陈蔚涛，武智，夏雨果，杨计平，李跃飞，李捷. 基于环境DNA技术的珠江中下游鱼类多样性初步研究. 南方水产科学. 2024(01): 120-129 . 本站查看
    4. 于耀鲜，邢冉冉，邓婷婷，王琳，卢思远，王宏勋，王丽梅，陈颖. 基于重组酶介导等温扩增-CRISPR/Cas12a的青鱼物种快速鉴定研究. 食品安全质量检测学报. 2024(04): 40-48 .
    5. 徐颖琪，梁绪虹，陈新军，宋成辉，彭祖焜，王丛丛. 基于COⅠ基因构建西北太平洋常见鱼类DNA条形码参考数据库. 上海海洋大学学报. 2024(04): 823-835 .
    6. 张浩博，王晓艳，陈治，钟兰萍，高天翔. 基于环境DNA metabarcoding的舟山及其邻近海域鱼类空间分布格局的初步研究. 水产学报. 2024(08): 125-138 .
    7. 李筱芹，吴开阳，倪达富，杨丽亚，鲁桃秀，张连博，邓华堂，吴彤飞，何荣超，付梅，姚维志，吕红健. 基于环境DNA技术的梯级水坝对长江上游重要支流鱼类多样性的影响研究——以綦江为例. 生态学报. 2024(19): 8865-8883 .
    8. 陈炳耀，信誉，路方婷，孙婧，刘杉，李敏，吴斌，王崇瑞，张衡，周玉香，白玲. 长江江豚监测现状及展望. 中国环境监测. 2023(02): 1-10 .
    9. 陈治，蔡杏伟，申志新，张清凤，李芳远，谷圆，李高俊，赵光军，王镇江. 海南岛淡水鱼类eDNA宏条形码COⅠ通用引物的筛选. 渔业科学进展. 2023(06): 40-57 .
    10. 刘乙蒙，刘洋，刘必林，俞晔伟，宋成辉，王丛丛. Cyt b和12S rRNA基因条形码在灯笼鱼科鱼类物种鉴定中的应用. 中国水产科学. 2023(09): 1112-1126 .
    11. 蒋佩文，李敏，张帅，陈作志，徐姗楠. 基于环境DNA宏条码和底拖网的珠江河口鱼类多样性. 水生生物学报. 2022(11): 1701-1711 .

    Other cited types(5)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(16)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return