Volume 20 Issue 4
Aug.  2024
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YANG Yu, ZHENG Wenwen, YU Wenbing, XU Yingjie, ZHANG Xing, SONG Xuehong, QIN Fenju. Study on bacteriostatic activity of nanocerium dioxide against two aquatic pathogenic Vibrio spp.[J]. South China Fisheries Science, 2024, 20(4): 144-153. DOI: 10.12131/20240046
Citation: YANG Yu, ZHENG Wenwen, YU Wenbing, XU Yingjie, ZHANG Xing, SONG Xuehong, QIN Fenju. Study on bacteriostatic activity of nanocerium dioxide against two aquatic pathogenic Vibrio spp.[J]. South China Fisheries Science, 2024, 20(4): 144-153. DOI: 10.12131/20240046
shu

Study on bacteriostatic activity of nanocerium dioxide against two aquatic pathogenic Vibrio spp.

  • 1.

    School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou 215009, China

  • 2.

    School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China

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  • Received Date: March 07, 2024
  • Revised Date: May 06, 2024
  • Accepted Date: May 21, 2024
  • Available Online: May 31, 2024
    Graphical Abstract
    • Abstract

  • Nanocerium dioxide (nCeO2) is an important new type of rare earth metal oxide with high oxidation resistance. In order to develop new antimicrobial agents witch are efficient, environmentally friendly and non-resistant, we obtained nCeO2 from cerium nitrate hexahydrate by high temperature calcination. We sudied the in vitro antibacterial activity of nCeO2 on two kinds of aquatic pathogens (Vibrio vulnificus and V. parahaemolyticus), and verified the antibacterial effect of nCeO2 by in vivo experiment of Macrobrachium rosenbergii. The results show that: 1) The obtained nCeO2 was relatively pure. The particle diameter was 25.03 nm, the Zeta potential (mV) was −5.65, and the dispersion in the solution was relatively uniform. 2) The minimum inhibitory concentrations (MIC) of nCeO2 against V. vulnificus and V. parahaemolyticus were 128 and 256 μg·mL−1, respectively, and the minimum bactericidal concentrations (MBC) all were 512 μg·mL−1. The diameter of the inhibition ring was 21.50 mm for V. vulnificus and 17.42 mm for V. parahaemolyticus. 3) The three concentrations of nCeO2 (1/2 MIC, 1 MIC and 2 MIC) all significantly improved the extracellular AKP activity, relative conductivity of bacterial solution, nucleic acid content and soluble protein content in the extracellular (p<0.05). 4) The nCeO2 supplementation in the feed significantly reduced the mortality of M. rosenbrei in V. vulnificus and V. parahaemolyticus infection models (p<0.05). The results indicate that nCeO2 has a good inhibitory effect on aquatic pathogens V. vulnificus and V. parahaemolyticus, and the antibacterial action may be related to its destruction of cell wall membrane integrity and cell membrane permeability.

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    • References (40)
  • [1]
    庞广富. 基于PLC物联网的自动化仪表在水产养殖中水质检测的应用[J]. 中国农业资源与区划, 2023, 44(8): 133, 144.
    [2]
    邓益琴. 水产动物弧菌病及其生物防治研究进展[J]. 大连海洋大学学报, 2023, 38(4): 553-563.
    [3]
    BERNÁLDEZ-SARABIA J, LIZáRRAGA-PARTIDA M L, HERNÁNDEZ-LÓPEZ E L, et al. Distribution of pathogenic vibrios and Vibrio spp. in the water column and sediment samples from the southern Gulf of Mexico[J]. Mar Pollut Bull, 2021, 173(Pt B): 113116.
    [4]
    彭钟琴, 黄璐. 水产品中创伤弧菌检测方法的研究进展[J]. 食品安全导刊, 2021(36): 190-192. doi: 10.3969/j.issn.1674-0270.2021.36.spaqdk202136067
    [5]
    孙一享, 常洪军, 杨行鑫, 等. 水产动物副溶血弧菌病及其噬菌体防治研究进展[J]. 微生物学通报, 2023, 50(8): 3620-3634.
    [6]
    BAKER-AUSTIN C, OLIVER J D. Vibrio vulnificus[J]. Trends Microbiol, 2020, 28(1): 81-82. doi: 10.1016/j.tim.2019.08.006
    [7]
    CHOI G, CHOI S H. Complex regulatory networks of virulence factors in Vibrio vulnificus[J]. Trends Microbiol, 2022, 30(12): 1205-1216. doi: 10.1016/j.tim.2022.05.009
    [8]
    陈媛媛, 王亚男, 刘士朋. 副溶血性弧菌致病性和检测方法研究进展[J]. 河北医药, 2022, 44(22): 3491-3495. doi: 10.3969/j.issn.1002-7386.2022.22.034
    [9]
    STEPHEN INBARAJ B, CHEN B H. An overview on recent in vivo biological application of cerium oxide nanoparticles[J]. Asian J Pharm Sci, 2020, 15(5): 558-575. doi: 10.1016/j.ajps.2019.10.005
    [10]
    ZHANG M Z, ZHANG C, ZHAI X Y, et al. Antibacterial mechanism and activity of cerium oxide nanoparticles[J]. Sci China Mater, 2019, 62(11): 1727-1739. doi: 10.1007/s40843-019-9471-7
    [11]
    ALPASLAN E, GEILICH B M, YAZICI H, et al. PH-controlled cerium oxide nanoparticle inhibition of both gram-positive and gram-negative bacteria growth[J]. Sci Rep, 2017, 7(1): 45859. doi: 10.1038/srep45859
    [12]
    高晓华, 张明辉. 40种中草药及其联合应用对凡纳滨对虾源副溶血弧菌的体外抑菌作用[J]. 甘肃农业大学学报, 2023, 58(2): 23-31, 41.
    [13]
    祁子麟, 陈咏琪, 蔡延渠, 等. 藤茶提取物及活性成分对创伤弧菌的体外抑菌活性及作用机制[J]. 广东药科大学学报, 2023, 39(1): 95-99.
    [14]
    刘静, 谢朋飞, 蔡延渠. 茶皂素对临床常见致病菌的抗菌活性及作用机制研究[J]. 广东药科大学学报, 2021, 37(5): 35-41.
    [15]
    陈雪琴, 赵圆圆, 张珍, 等. 肉桂精油的化学成分分析及其对沙门氏菌细胞膜损伤机制的研究[J]. 食品与发酵工业, 2022, 48(14): 24-32.
    [16]
    孔嘉明, 戴习林, 黎兰诗, 等. 副溶血弧菌对罗氏沼虾肝胰腺和鳃组织中呼吸相关酶活性及抗氧化酶基因表达的影响[J]. 南方农业学报, 2021, 52(12): 3294-3302. doi: 10.3969/j.issn.2095-1191.2021.12.012
    [17]
    YILMAZ S, YILMAZ E, DAWOOD M A O, et al. Probiotics, prebiotics, and synbiotics used to control vibriosis in fish: a review[J]. Aquaculture, 2022(547): 737514.
    [18]
    WITT L S, SPICER J O, BURD E, et al. Evaluation of clinicians' knowledge and use of minimum inhibitory concentration values[J]. Braz J Infect Dis, 2021, 25(6): 101656. doi: 10.1016/j.bjid.2021.101656
    [19]
    PARVEKAR P, PALASKAR J, METGUD S, et al. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus[J]. Biomater invest Dent, 2020, 7(1): 105-109.
    [20]
    QUIROZ-GUZMÁN E, MORREEUW Z P, PEÑA-RODRÍGUEZ A, et al. Flavonoid-enriched extract of Agave lechuguilla bagasse as a feed supplement to prevent vibriosis in Pacific white shrimp Penaeus vannamei[J]. Aquaculture, 2023, 562: 738867. doi: 10.1016/j.aquaculture.2022.738867
    [21]
    VINU D, GOVINDARAJU K, VASANTHARAJA R, et al. Biogenic zinc oxide, copper oxide and selenium nanoparticles: preparation, characterization and their anti-bacterial activity against Vibrio parahaemolyticus[J]. J. Nanostruct Chem, 2021, 11(2): 271-286. doi: 10.1007/s40097-020-00365-7
    [22]
    陈秀霞, 池洪树, 许斌福, 等. 创伤弧菌浸泡感染对孔雀鱼的影响[J]. 福建畜牧兽医, 2019, 41(2): 11-14. doi: 10.3969/j.issn.1003-4331.2019.02.002
    [23]
    张坤. 大黄鱼主要病原菌的分离鉴定与抗菌复方开发[D]. 厦门: 集美大学, 2016: 24.
    [24]
    CHEN D D, GUO L F, YI C, et al. Hepatopancreatic transcriptome analysis and humoral immune factor assays in red claw crayfish (Cherax quadricarinatus) provide insight into innate immunomodulation under Vibrio parahaemolyticus infection[J]. Ecotoxicol Environ Saf, 2021, 217: 112266. doi: 10.1016/j.ecoenv.2021.112266
    [25]
    QIN F J, SHEN T, YANG H X, et al. Dietary nano cerium oxide promotes growth, relieves ammonia nitrogen stress, and improves immunity in crab (Eriocheir sinensis)[J]. Fish Shellfish Immunol, 2019, 92: 367-376. doi: 10.1016/j.fsi.2019.06.019
    [26]
    芦平, 刘欢, 肖苗, 等. 原儿茶酸对副溶血弧菌的抑菌和减毒作用[J]. 现代食品科技, 2022, 38(5): 278-285.
    [27]
    XIA H, YANG H J, YAN N, et al. Bacteriostatic effects of phage F23s1 and its endolysin on Vibrio parahaemolyticus[J]. J Basic Microbiol, 2022, 62(8): 963-974. doi: 10.1002/jobm.202200056
    [28]
    PÉREZ P, RIBAS J C. Cell wall analysis[J]. Methods, 2004, 33(3): 245-251. doi: 10.1016/j.ymeth.2003.11.020
    [29]
    王意, 张博涵, 陆一鸣, 等. 黄芩提取物对嗜水气单胞菌抑菌活性和机制的研究[J]. 陕西科技大学学报, 2019, 37(6): 73-78, 104. doi: 10.3969/j.issn.1000-5811.2019.06.012
    [30]
    KANG S, FANHUASHI, XINYANGHAN, et al. Antibacterial activity and mechanism of lactobionic acid against Pseudomonas fluorescens and Methicillin-resistant Staphylococcus aureus and its application on whole milk[J]. Food Control, 2020, 108: 106876. doi: 10.1016/j.foodcont.2019.106876
    [31]
    杨维琴, 钱亮亮, 张宗艺, 等. 黄连提取物抗副溶血弧菌活性成分的鉴定及其作用机理[J]. 中国食品添加剂, 2022, 33(4): 181-187.
    [32]
    IQBAL N, ANASTASIOU A, ASLAM Z, et al. Interrelationships between the structural, spectroscopic, and antibacterial properties of nanoscale (<50 nm) cerium oxides[J]. Sci Rep, 2021, 11(1): 20875. doi: 10.1038/s41598-021-00222-9
    [33]
    WAGNER B M. Crossroads of the basic sciences-the cell membrane[J]. Hum Pathol, 1984, 15(11): 1007. doi: 10.1016/S0046-8177(84)80241-5
    [34]
    EOM S H, LEE D S, JUNG Y J, et al. The mechanism of antibacterial activity of phlorofucofuroeckol-A against methicillin-resistant Staphylococcus aureus[J]. Appl Microbiol Biotechnol, 2014, 98(23): 9795-9804. doi: 10.1007/s00253-014-6041-8
    [35]
    KRISHNAMURTHI V R, ROGERS A, PEIFER J, et al. Microampere electric current causes bacterial membrane damage and two-way leakage in a short period of time[J]. Appl Environ Microbiol, 2020, 86(16): e01015-20.
    [36]
    蔡延渠, 李苑新, 鲁湘鄂. 诃子叶提取物对水产养殖常见致病性弧菌的抗菌作用研究[J]. 饲料研究, 2022, 45(21): 78-81.
    [37]
    ZAMANI K, ALLAH-BAKHSHI N, AKHAVAN F, el al. Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginos[J]. BMC Biotechnol, 2021, 21(1): 68.

    ZAMANI K, ALLAH-BAKHSHI N, AKHAVAN F, el al. Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginos[J]. BMC Biotechnol, 2021, 21(1): 68.
    [38]
    孙元琛, 徐冰洁, 曹艺筹, 等. 饲料中添加纳米氧化铈对氨氮与嗜水气单胞菌胁迫下中华绒螯蟹的保护效应[J]. 南方水产科学, 2022, 18(3): 94-101.
    [39]
    REDLING K. Rare earth elements in agriculture with emphasis on animal husbandry[J]. Ludwig-Maximilians-Universität München, 2006: 325.
    [40]
    PAEMEL M V , DIERICK N , JANSSENS G, et al. Selected trace and ultratrace elements: biological role, content in feed and requirements in animal nutrition-elements for risk assessment[J]. Efsa Supporting Publ, 2010, 7(7): 68E.
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