Volume 20 Issue 2
Apr.  2024
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TAN Zeyu, LI Tao, JIANG Jingzhe, HUANG Xiaolin, YANG Yukai. Observation of embryo and morphological development of larval, juvenile and young speckled snowmark clownfish (Amphiprion sp.)[J]. South China Fisheries Science, 2024, 20(2): 73-82. DOI: 10.12131/20230119
Citation: TAN Zeyu, LI Tao, JIANG Jingzhe, HUANG Xiaolin, YANG Yukai. Observation of embryo and morphological development of larval, juvenile and young speckled snowmark clownfish (Amphiprion sp.)[J]. South China Fisheries Science, 2024, 20(2): 73-82. DOI: 10.12131/20230119
shu

Observation of embryo and morphological development of larval, juvenile and young speckled snowmark clownfish (Amphiprion sp.)

  • 1.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

  • 2.

    College of Aquatic and Life Sciences, Dalian Ocean University, Dalian 116023, China

  • 3.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Research Exploitation & Utilization, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

More Information
  • Received Date: June 20, 2023
  • Revised Date: September 19, 2023
  • Accepted Date: November 25, 2023
  • Available Online: November 28, 2023
    Graphical Abstract
    • Abstract

  • In order to improve the artificial breeding technology of the speckled snowmark clownfish (Amphiprion sp.), we observed the breeding habits, embryonic development and post-embryonic development under artificial simulated natural conditions. The results show that the fertilized eggs were 1.9−2.1 mm in long diameter and 0.93−0.95 mm in short diameter. The embryonic development mainly went through sperm-egg stage, embryonic disc stage, oogenesis stage, blastocyst stage, protointestinal stage, neuroembryonic stage, organ formation stage and hatching stage. At the water temperature of (27±0.5) ℃, it took approximately 197 h for the fertilized egg to hatch out of the membrane. The newly hatched larvae had a total length of (4.415±0.412) mm, a transparent body, obvious fin membranes, and only a small portion of the yolk sac remained, which had been opened for feeding. Larval stage: At 9 days after hatching [DAH, body length of (6.813±0.835) mm)], the fin membrane disappeared and the fins formed, and the banding on the head appeared. Juvenile stage: at 21 DAH [Body length of (10.581±2.659 mm)], accompanied by scale formation on the body, the differentiation of body colour and stripe banding started to appear. Young stage: 35 DAH [Body length of (15.512±4.927) mm], four different strains with different body colour patterns were easily identified. In brief, as an artificially selected new variety, the most obvious feature during the post-embryonic development of speckled snowmark clownfish is formation and differentiation of body colour patterns such as stripe banding during juvenile stage.

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