Volume 19 Issue 6
Dec.  2023
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WU Xiaopeng, HUANG Minwei, CHEN Xiaoying, PENG Kai, ZHAO Jichen, ZHONG Ping, LIU Fengkun, ZHANG Yehui, HUANG Wen. Transcriptome analysis of metamorphosis stage of Holothuria leucospilota[J]. South China Fisheries Science, 2023, 19(6): 84-96. DOI: 10.12131/20230105
Citation: WU Xiaopeng, HUANG Minwei, CHEN Xiaoying, PENG Kai, ZHAO Jichen, ZHONG Ping, LIU Fengkun, ZHANG Yehui, HUANG Wen. Transcriptome analysis of metamorphosis stage of Holothuria leucospilota[J]. South China Fisheries Science, 2023, 19(6): 84-96. DOI: 10.12131/20230105
shu

Transcriptome analysis of metamorphosis stage of Holothuria leucospilota

  • 1.

    College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China

  • 2.

    Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

  • 3.

    Collaborative Innovation Center of Aquatic Sciences, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

  • 4.

    College of Life Science and Technology, Jinan University, Guangzhou 510632, China

  • 5.

    Sericulture & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

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  • Received Date: May 18, 2023
  • Revised Date: July 17, 2023
  • Accepted Date: July 20, 2023
  • Available Online: August 06, 2023
    Graphical Abstract
    • Abstract

  • Development of sea cucumber larvae needs to go through stages of auricularia, doliolaria, pentactula and juvenile. High mortality rate from metamorphosis and development of planktonic larvae to attachment stage of seedlings is a common problem in tropical sea cucumbers breeding. However, the gene regulation mechanism underlying the metamorphosis development of tropical sea cucumber is still unclear. In this study, we chose larval Holothuria leucospilota from four periods of early auricularia (A), mid auricularia (B), late auricularia (C) and doliolaria (D) as samples for high-throughput transcriptome sequencing to investigate the molecular mechanism underlying its metamorphosis development. The results show that a total of 83.6 GB Raw reads were generated, and 93 528 Unigenes were obtained by splicing. Pairwise comparisons between adjacent groups of the four sequencing libraries show that the number of genes with significant differential expression in A_vs_B, B_vs_C and C_vs_D were 17 732, 11 757 and 11 319, respectively. GO function enrichment shows that differential genes were mainly enriched in GO functions related to cell growth, such as molecular function and catalytic activity. In addition, KEGG pathways were analyzed, and the results show that the differential genes were significantly enriched in pathways related to cell differentiation, proliferation and apoptosis, such as PI3K-Akt, cell cycle and cancer pathways. Among them, the enrichment frequency of the pathway in cancer significantly increased during the metamorphosis process, which indicates a key role in the transformation of larval growth and development mode. The screened differentially expressed genes and predicted functional information can lay a foundation for the research on the regulatory mechanism of growth and development, artificial breeding and molecular improvement application of H. leucospilota.

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  • [1]
    中国科学院中国动物志编辑委员会等. 中国动物志 棘皮动物门 海参纲[M]. 北京: 科学出版社, 1997: 109-110.
    [2]
    姚雪梅. 热带海参在我国南方沿海地区的增养殖前景[J]. 水产文摘, 2004(4): 2-5.
    [3]
    HUANG W, HUO D, YU Z H, et al. Spawning, larval development and juvenile growth of the tropical sea cucumber Holothuria leucospilota[J]. Aquaculture, 2018, 488: 22-29. doi: 10.1016/j.aquaculture.2018.01.013
    [4]
    于宗赫, 黄文, 马文刚, 等. 牟氏角毛藻和海洋红酵母对玉足海参浮游幼体发育、生长及成活率的影响[J]. 水产学报, 2021, 45(12): 2003-2010.
    [5]
    郑杰, 宋志远, 吴海涛, 等. 响应面法优化海参体壁自溶条件及其影响因素分析[J]. 食品安全质量检测学报, 2018, 9(22): 5981-5986.
    [6]
    FERGUSON C E, BENNETT N J, KOSTKA W, et al. The tragedy of the commodity is not inevitable: indigenous resistance prevents high-value fisheries collapse in the Pacific islands[J]. Global Environ Change, 2022, 73: 102477. doi: 10.1016/j.gloenvcha.2022.102477
    [7]
    HAWAS U W, ABOU EL-KASSEM L T, SHAHER F M, et al. Sulfated triterpene glycosides from the Saudi Red Sea cucumber Holothuria atra with antioxidant and cytotoxic activities[J]. Thalassas: An Int J Mar Sci, 2021, 37(2): 817-824. doi: 10.1007/s41208-021-00305-4
    [8]
    ZHAO Y C, XUE C H, ZHANG T T, et al. Saponins from sea cucumber and their biological activities[J]. J Agric Food Chem, 2018, 66(28): 7222-7237. doi: 10.1021/acs.jafc.8b01770
    [9]
    LU Z Q, SUN N, DONG L, et al. Production of bioactive peptides from sea cucumber and its potential health benefits: a comprehensive review[J]. J Agric Food Chem, 2022, 70(25): 7607-7625. doi: 10.1021/acs.jafc.2c02696
    [10]
    LI C L, ZHAO W, QIN C X, et al. Comparative transcriptome analysis reveals changes in gene expression in sea cucumber (Holothuria leucospilota) in response to acute temperature stress[J]. Comp Biochem Physiol D, 2021, 40: 100883.
    [11]
    PURCELL S W, MERCIER A, CONAND C, et al. Sea cucumber fisheries: global analysis of stocks, management measures and drivers of overfishing[J]. Fish Fish, 2013, 14(1): 34-59. doi: 10.1111/j.1467-2979.2011.00443.x
    [12]
    谭春明, 赵旺, 马振华, 等. 红腹海参消化道指标、组织学和酶活性的季节变化[J]. 南方水产科学, 2022, 18(5): 39-45.
    [13]
    RAKAJ A, FIANCHINI A, BONCAGNI P, et al. Spawning and rearing of Holothuria tubulosa: a new candidate for aquaculture in the Mediterranean region[J]. Aquac Res, 2018, 49(1): 557-568. doi: 10.1111/are.13487
    [14]
    RAKAJ A, FIANCHINI A, BONCAGNI P, et al. Artificial reproduction of Holothuria polii: a new candidate for aquaculture[J]. Aquaculture, 2019, 498: 444-453. doi: 10.1016/j.aquaculture.2018.08.060
    [15]
    HU C Q, LI H P, XIA J J, et al. Spawning, larval development and juvenile growth of the sea cucumber Stichopus horrens[J]. Aquaculture, 2013, 404: 47-54.
    [16]
    冯永勤, 翁文明, 方再光, 等. 糙海参苗种规模化繁育技术研究[J]. 水产科学, 2021, 40(5): 750-756.
    [17]
    黄星美, 赵旺, 李长林, 等. 盐度对玉足海参浮游幼体生长的影响[J]. 南方水产科学, 2022, 18(3): 111-117.
    [18]
    宋汝浩, 胡瑞芹, 李根芳, 等. 基于转录组学技术对斑马鱼肝脏组织低氧胁迫影响的研究[J]. 南方水产科学, 2022, 18(6): 60-68.
    [19]
    ZHAO J C, CHEN X Y, HE Z H, et al. Transcriptome analysis provides new insights into host response to Hepatopancreatic necrosis disease in the black tiger shrimp Penaeus monodon[J]. J Ocean Univ China, 2021, 20: 1183-1194. doi: 10.1007/s11802-021-4744-x
    [20]
    范嗣刚, 杨文燕, 黄皓, 等. 不同生长速率花鲈肌肉转录组分析及生长相关基因筛选[J]. 广东海洋大学学报, 2022, 42(5): 9-17.
    [21]
    LIN X Y, DENG S Z, LIU X D, et al. Comparative transcriptome analysis of Phascolosoma esculenta under different salinities[J]. Aquac Fish, 2020, 5(6): 323-330. doi: 10.1016/j.aaf.2020.06.013
    [22]
    BYRNE M, KOOP D, STRBENAC D, et al. Transcriptomic analysis of sea star development through metamorphosis to the highly derived pentameral body plan with a focus on neural transcription factors[J]. DNA Res, 2020, 27(1): dsaa007. doi: 10.1093/dnares/dsaa007
    [23]
    CONACO C, NEVEU P, ZHOU H J, et al. Transcriptome profiling of the demosponge Amphimedon queenslandica reveals genomewide events that accompany major life cycle transitions[J]. BMC Genomics, 2012, 13: 1-19. doi: 10.1186/1471-2164-13-1
    [24]
    BORAH B K, HAUZEL L, RENTHLEI Z, et al. Photic and non-photic regulation of growth, development, and metamorphosis in giant tree frog (Rhacophorus maximus) tadpoles[J]. Biol Rhythm Res, 2018, 49(6): 955-967.
    [25]
    HODIN J, HEYLAND A, MERCIER A, et al. Culturing echinoderm larvae through metamorphosis[J]. Methods Cell Biol, 2019, 150: 125-169.
    [26]
    陈雪峰, 王春琳, 顾志敏, 等. 罗氏沼虾(Macrobrachium rosenbergii)卵巢发育不同时期转录组分析[J]. 海洋与湖沼, 2019, 50(2): 398-408.
    [27]
    YIN J Y, LI Z B, PAN C H, et al. Understanding the internal differences behind unsynchronized growth in sea cucumber Holothuria leucospilota by integration of transcriptomic and metabolomic data[J]. Aquac Rep, 2023, 32: 101688.
    [28]
    ZAKERI Z, LOCKSHIN R A. Cell death during development[J]. J Immunol Methods, 2002, 265(1/2): 3-20.
    [29]
    NANUT M P, FONOVIĆ U P, JAKOŠ T, et al. The role of cysteine peptidases in hematopoietic stem cell differentiation and modulation of immune system function[J]. Front Immunol, 2021, 12: 680279. doi: 10.3389/fimmu.2021.680279
    [30]
    XIE J, SUN Y, CAO Y, et al. Transcriptomic and metabolomic analyses provide insights into the growth and development advantages of triploid Apostichopus japonicus[J]. Mar Biotechnol, 2022, 24(1): 151-162. doi: 10.1007/s10126-022-10093-4
    [31]
    ASADI M, TAGHIZADEH S, KAVIANI E, et al. Caspase-3: structure, function, and biotechnological aspects[J]. Biotechnol Appl Biochem, 2022, 69(4): 1633-1645. doi: 10.1002/bab.2233
    [32]
    MCCOMB S, CHAN P K, GUINOT A, et al. Efficient apoptosis requires feedback amplification of upstream apoptotic signals by effector caspase-3 or-7[J]. Sci Adv, 2019, 5(7): eaau9433. doi: 10.1126/sciadv.aau9433
    [33]
    CHAMBON J P, SOULE J, POMIES P, et al. Tail regression in Ciona intestinalis (Prochordate) involves a caspase-dependent apoptosis event associated with ERK activation[J]. Development, 2002, 129(13): 3105-3114.
    [34]
    AIELLO D, PATEL K, LASAGNA E. The myostatin gene: an overview of mechanisms of action and its relevance to livestock animals[J]. Anim Genet, 2018, 49(6): 505-519. doi: 10.1111/age.12696
    [35]
    HOOGAARS W M H, JASPERS R T. Past, present, and future perspective of targeting myostatin and related signaling pathways to counteract muscle atrophy[J]. Muscle Atrophy, 2018, 1088: 153-206.
    [36]
    杨慧荣, 曾泽乾, 杨炎, 等. 鱼类肌肉生长抑制素myostatin研究进展[J]. 中山大学学报(自然科学版)(中英文), 2022, 61(5): 1-8.
    [37]
    陈素华, 吴杨平, 陈爱华, 等. 文蛤CDK1基因在选育与自然群体早期生长阶段中的表达特征[J]. 中国水产科学, 2020, 27(9): 1042-1051.
    [38]
    MOMOSE T, DERELLE R, HOULISTON E. A maternally localised Wnt ligand required for axial patterning in the cnidarian Clytia hemisphaerica[J]. Development, 2008, 135: 2105-2113. doi: 10.1242/dev.021543
    [39]
    TEO R, MÖHRLEN F, PLICKERT G, et al. An evolutionary conserved role of Wnt signaling in stem cell fate decision[J]. Dev Biol, 2006, 289(1): 91-99. doi: 10.1016/j.ydbio.2005.10.009
    [40]
    江红霞, 刘慧芬, 马晓, 等. 转录组测序筛选克氏原螯虾卵巢发育、免疫和生长相关基因[J]. 水产学报, 2021, 45(3): 396-414.
    [41]
    JERE S W, HOURELD N N, ABRAHAMSE H. Role of the PI3K/AKT (mTOR and GSK3β) signalling pathway and photobiomodulation in dia-betic wound healing[J]. Cytokine Growth Factor Rev, 2019, 50: 52-59.
    [42]
    TANG B L. Neuroprotection by glucose-6-phosphate dehydrogenase and the pentose phosphate pathway[J]. J Cell Biochem, 2019, 120(9): 14285-14295.
    [43]
    丁丹, 潘宝平, 王玉梅, 等. 青蛤 (Cyclina sinensis) AP-1基因的克隆及在鳗弧菌 (Vibrio anguillarum) 侵染下的表达分析[J]. 海洋与湖沼, 2018, 49(1): 192-197.
    [44]
    BACKES T M, RÖSSLER O G, HUI X, et al. Stimulation of TRPV1 channels activates the AP-1 transcription factor[J]. Biochem Pharmacol, 2018, 150: 160-169. doi: 10.1016/j.bcp.2018.02.008
    [45]
    马清花, 陈雪妍, 卫唯, 等. 青海湖裸鲤AP-1基因的克隆与表达分析[J]. 基因组学与应用生物学, 2020, 39(7): 2964-2971.
    [46]
    CHEUNG C H A, CHANG Y C, LIN T Y, et al. Anti-apoptotic proteins in the autophagic world: an update on functions of XIAP, Survivin, and BRUCE[J]. J Biomed Sci, 2020, 27(1): 1-10. doi: 10.1186/s12929-019-0592-z
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