Volume 19 Issue 6
Dec.  2023
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YANG Zhigang, JIANG Qingqing, FANG Yucheng, CHEN Aqin, CHENG Yongxu, WANG Aimin. Synergistic effects of pH and phenanthrene on toxicity of juvenile Chinese mitten crab (Eriocheir sinensis)[J]. South China Fisheries Science, 2023, 19(6): 142-149. DOI: 10.12131/20230068
Citation: YANG Zhigang, JIANG Qingqing, FANG Yucheng, CHEN Aqin, CHENG Yongxu, WANG Aimin. Synergistic effects of pH and phenanthrene on toxicity of juvenile Chinese mitten crab (Eriocheir sinensis)[J]. South China Fisheries Science, 2023, 19(6): 142-149. DOI: 10.12131/20230068
shu

Synergistic effects of pH and phenanthrene on toxicity of juvenile Chinese mitten crab (Eriocheir sinensis)

  • 1.

    Shanghai Ocean University/Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China

  • 2.

    Shanghai Ocean University/Center for Research on Environmental Ecology and Fish Nutrion (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai 201306, China

  • 3.

    Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306, China

  • 4.

    College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224000, China

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  • Received Date: April 03, 2023
  • Revised Date: May 31, 2023
  • Accepted Date: June 28, 2023
  • Available Online: July 06, 2023
    Graphical Abstract
    • Abstract

  • In order to explore the synergistic effects of acidification and environmental pollutant phenanthrene (PHE) on Chinese mitten crabs (Eriocheir sinensis), we carried out a 14-day joint exposure experiment of E. sinensis [(15.0±2.3) g] to different pH (5.5, 6.5, 7.8) and PHE (0, 50 µg·L−1). The results show that: 1) Under the synergistic treatment of pH 5.5×PHE 50 on E. sinensis, the hepatopancreas and gill tissues were severely damaged; the shape of the hepatopancreatic tubules changed; a large number of vacuoles appeared in the atrophy of the hepatic duct cells. The gill axis was enlarged; the cuticle of the gill filament was damaged; the local basement membrane was ruptured. However, pH 6.5×PHE 50 did not significantly damage the hepatopancreas and gill tissues of E. sinensis. 2) PHE treatment significantly reduced the contents of Gly and LDL-C in hepatopancreas of juvenile E. sinensis, indicating that PHE could decrease the energy metabolism level of E. sinensis. Compared with the control group, pH 5.5×PHE 50 inhibited the contents of TG, Gly, HDL-C and LDL-C, while pH 6.5×PHE 50 significantly increased TG content. 3) Real-time fluorescent quantitative PCR experiment shows that AHR, ARNT and CYP1A1 increased significantly with the decrease of pH, and reached the highest values at pH 5.5. The results reveal that the combining effect of acidification and PHE changes the energy metabolism of E. sinensis, and pH 5.5 significantly aggravates the toxic effect of PHE on the hepatopancreas tissue structure of E. sinensis, as well as its energy metabolism.

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