Untargeted metabolomics analysis of metabolic differences of crayfish (<i>Procambarus clarkii</i>)  meat with different diets

BAO Junjie; WANG Yongjie; CHEN Honglian; SUN Wen; ZHANG Jing; ZHOU Beibei

doi:10.12131/20230055

To provide references for improving the quality of crayfish (Procambarus clarkii) meat, we studied the differences in muscle metabolites of crayfish fed with two diets by a non-targeted metabolomic method using liquid chromatography mass spectrometry. Different metabolites in meat were screened by principal components analysis (PCA), cluster analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The results show that 27 significantly different metabolites were screened under the positive and negative ion modes. Compared with the control group (General feed), the contents of adenylsuccinic acid, phosphatidylcholine, quinate, psoralen, phosphatidylserine and glutamic in the experimental group (Fermented feed) increased significantly. According to KEGG pathway analysis, the top four pathways with the highest concentration of metabolites were histidine metabolism pathway, arginine proline metabolism pathway, protein digestion and absorption metabolism pathway, as well as -tRNA synthesis pathway. The results indicate that diets play a positive role in regulating amino acid metabolism, protein synthesis and assisting in the synthesis of aminoacyl-tRNA enzymes in organisms.

[1]	于秀娟, 郝向举, 党子乔, 等. 中国小龙虾产业发展报告 (2022)[J]. 中国水产, 2022(6): 47-54. doi: 10.3969/j.issn.1002-6681.2022.6.zhongguosc202206017
[2]	陈实, 吴旭干, 杨丰, 等. 配合饲料和传统饵料养殖脊尾白虾气味品质的比较[J]. 食品工业科技, 2020, 41(1): 189-194, 200.
[3]	CHANDRAN J, BELLAD A, RAMARAJAN M G, et al. Applications of quantitative metabolomics to revolutionize early diagnosis of inborn errors of metabolism in India[J]. Anal Sci Adv, 2021, 2(11/12): 546-563.
[4]	DUBUIS S, BAENKE F, SCHERBICHLER N, et al. Metabotypes of breast cancer cell lines revealed by non-targeted metabolomics[J]. Metab Eng, 2017, 43(Pt B): 173-186.
[5]	PAN Y, GU H W, LV Y, et al. Untargeted metabolomic analysis of Chinese red wines for geographical origin traceability by UPLC-QTOF-MS coupled with chemometrics[J]. Food Chem, 2022, 394: 133473. doi: 10.1016/j.foodchem.2022.133473
[6]	WU CH C, MA Y J, WANG D, et al. Integrated microbiology and metabolomics analysis reveal plastic mulch film residue affects soil microorganisms and their metabolic functions[J]. J Hazard Mater, 2022, 423(PB): 127258.
[7]	李玮, 贾婧怡, 李龙, 等. 核磁共振代谢组学技术鉴别天然奶油与人造奶油[J]. 食品科学, 2017, 38(12): 278-285.
[8]	高淑芳, 张金鹏, 施永海, 等. 基于LC-MS技术的海、淡水养殖刀鲚卵巢的代谢组学比较分析[J]. 南方水产科学, 2022, 18(3): 68-75. doi: 10.12131/20210185
[9]	张舒, 王长远, 冯玉超, 等. 气相色谱-质谱联用代谢组学技术分析不同产地稻米代谢物[J]. 食品科学, 2021, 42(8): 206-213.
[10]	林艳萍, 司端运, 刘昌孝. 液相色谱和质谱联用技术结合化学计量学应用于代谢组学的研究进展[J]. 分析化学, 2007(10): 1535-1540.
[11]	刘慧茹, 汪海洋, 王喆, 等. 基于代谢组学和斑马鱼模型探究西洋参抗疲劳的关键活性成分[J]. 药学学报, 2023, 58(4): 1024-1032.
[12]	张彦坤, 杨兵坤, 李航宇, 等. 饥饿胁迫下剑尾鱼肝脏代谢组学研究[J]. 四川动物, 2021, 40(6): 611-621. doi: 10.11984/j.issn.1000-7083.20210058
[13]	童铃, 金毅, 徐坤华, 等. 3种鲣鱼背部肌肉的营养成分分析及评价[J]. 南方水产科学, 2014, 10(5): 51-59. doi: 10.3969/j.issn.2095-0780.2014.05.008
[14]	虞为, 杨育凯, 林黑着, 等. 牛磺酸对花鲈生长性能、消化酶活性、抗氧化能力及免疫指标的影响[J]. 南方水产科学, 2021, 17(2): 78-86.
[15]	叶彬清, 陶宁萍, 王锡昌. 秋刀鱼肌肉营养成分分析及评价[J]. 营养学报, 2014, 36(4): 406-408.
[16]	邵俊杰, 钟立强, 朱昱璇, 等. 配合饲料和冰鲜鱼对大口黑鲈生长和品质的影响[J]. 水产科学, 2023, 42(1): 81-88. doi: 10.16378/j.cnki.1003-1111.21065
[17]	WEN D L, LIU Y, YU Q. Metabolomic approach to measuring quality of chilled chicken meat during storage[J]. Poultry Sci, 2020, 99(5): 2543-2554. doi: 10.1016/j.psj.2019.11.070
[18]	XU X Y, YANG H, XU Z, et al. The comparison of largemouth bass (Micropterus salmoides) fed trash fish and formula feeds: growth, flesh quality and metabolomics[J]. Front Nutr, 2022, 9: 966248. doi: 10.3389/fnut.2022.966248
[19]	ZHANG K K, AI Q H, MAI K S, et al. Effects of dietary hydroxyproline on growth performance, body composition, hydroxyproline and collagen concentrations in tissues in relation to prolyl 4-hydroxylaseα (I) gene expression of juvenile turbot (Scophthalmus maximus L. ) fed high plant protein diets[J]. Aquaculture, 2013, 404/405: 77-84. doi: 10.1016/j.aquaculture.2013.04.025
[20]	CARRAGHER J F, MÜHLHAUSLER B S, GEIER MS, et al. Effect of dietary ALA on growth rate, feed conversion ratio, mortality rate and breast meat omega-3 LCPUFA content in broiler chickens[J]. Anim Prod Sci, 2016, 56(5): 815-823. doi: 10.1071/AN14743
[21]	MURUGESAN A, HOLMSTEDT S, BROWN K C, et al. Design and synthesis of novel quinic acid derivatives: in vitro cytotoxicity and anticancer effect on glioblastoma[J]. Future Med Chem, 2020(21): 1891-1910.
[22]	李静, 陈冬梅, 陈明星. 亚麻木酚素小鼠体内抗氧化活性研究[J]. 中国粮油学报, 2012, 27(1): 48-52. doi: 10.3969/j.issn.1003-0174.2012.01.011
[23]	BENEDETTA C, LUISA D M, CARLA C, et al. Sphingolipids and the immune system[J]. Pharmacol Res, 2003, 47(5): 421-437. doi: 10.1016/S1043-6618(03)00051-3
[24]	SHIU W L, HUANG K R, HUNG J C, et al. Knockdown of zebrafish YY1a can downregulate the phosphatidylserine (PS) receptor expression, leading to induce the abnormal brain and heart development[J]. J Biomed Sci, 2016, 23(1): 31-33. doi: 10.1186/s12929-016-0248-1
[25]	RUBÉN D, MIRIAN P, MOHAMMED G, et al. A comprehensive review on lipid oxidation in meat and meat products[J]. Antioxidants, 2019, 8(10): 429. doi: 10.3390/antiox8100429
[26]	ZHENG X S, JI H W, ZHANG D, et al. The identification of three phospholipid species roles on the aroma formation of hot-air-dried shrimp (Litopenaeus vannamei) by gas chromatography-ion mobility spectrometry and gas chromatography-mass spectrometry[J]. Food Res Int, 2022, 162(PB): 112191.
[27]	RAO Z T, WANG S Q, WANG J Q. Protective effects of psoralidin on IL-1β-induced chondrocyte apoptosis.[J]. Mol Med Rep, 2018, 17(2): 3418-3424.
[28]	王晓艳, 李伟霞, 张辉, 等. 补骨脂及其主要成分对人正常肝细胞L02的损伤作用研究[J]. 中医研究, 2020, 33(4): 59-63. doi: 10.3969/j.issn.1001-6910.2020.04.24
[29]	刘刚, 冯美云, 张缨, 等. 补充苹果酸复合营养液对拳击运动员抗疲劳能力的影响[J]. 北京体育大学学报, 2009, 32(5): 47-49.
[30]	LI X Y, ZHENG S X, WU G Y. Nutrition and metabolism of glutamate and glutamine in fish[J]. Amino acids, 2020, 52(5): 671-691. doi: 10.1007/s00726-020-02851-2
[31]	JUN J, LONG Y, LI J Y, et al. Glutamate attenuates lipopolysaccharide-induced oxidative damage and mRNA expression changes of tight junction and defensin proteins, inflammatory and apoptosis response signaling molecules in the intestine of fish[J]. Fish Shellfish Immum, 2017, 70: 473-484. doi: 10.1016/j.fsi.2017.09.035
[32]	王珂雯, 徐雷, 徐贞贞, 等. 基于液相色谱-四极杆飞行时间质谱方法分析冰鲜鸡肉代谢标志物[J]. 食品科学, 2021, 42(16): 293-303. doi: 10.7506/spkx1002-6630-20200827-377
[33]	WANG Q C, XU Z, AI Q H. Arginine metabolism and its functions in growth, nutrient utilization, and immunonutrition of fish[J]. Anim Nutr, 2021, 7(3): 716-727. doi: 10.1016/j.aninu.2021.03.006
[34]	CHENG Z Y, CHEN S Q, AN M L, et al. Effects of replacing fish meal with soybean meal, with or without dietary arginine, on growth performance, immune indices and intestinal morphology of grouper, Epinephelus malabaricus[J]. Aquac Res, 2018, 49(9): 2954-2964. doi: 10.1111/are.13754
[35]	WANG B B, PAN X SH, JIA J, et al. Strategy and regulatory mechanisms of glutamate feeding to enhance astaxanthin yield in Xanthophyllomyces dendrorhous[J]. Enzyme Micro Tech, 2019, 125: 45-52. doi: 10.1016/j.enzmictec.2019.02.010

Cited by

Periodical cited type(5)

高铭悦，楚馨，刘思玉，姚嘉赟，蔺凌云，沈锦玉，潘晓艺. 大口黑鲈蛙虹彩病毒传代致弱株免疫效果评价. 水生生物学报. 2025(06): 23-33 .

黄仁善，钟纯怡，张帝，强俊，陶易凡，李岩，董亚伦，路思琪，徐跑. 不同大口黑鲈群体生长性能、肌肉营养成分、肠道形态结构及菌群组成的比较研究. 动物营养学报. 2024(02): 1158-1172 .

李硕，陈静妮，赵立宁，黄春萍，黄锦炉，王贵平，仲颖. 抗大口黑鲈蛙虹彩病毒卵黄抗体的制备及其间接ELISA检测方法的建立. 南方水产科学. 2024(02): 129-139 .

本站查看

傅建军，龚雅婷，朱文彬，强俊，张林兵，王兰梅，罗明坤，董在杰. 大口黑鲈微卫星多重PCR体系及3个群体的遗传分析. 水生生物学报. 2023(09): 1514-1522 .

张涛，周剑光，张林，甘金华，何力. 大口黑鲈形态特征、染色体核型及眼晶状体乳酸脱氢酶的电泳分析. 西北农业学报. 2023(10): 1503-1511 .

Other cited types(4)

Untargeted metabolomics analysis of metabolic differences of crayfish (Procambarus clarkii) meat with different diets

Abstract

References

Related Articles

Cited by

Periodical cited type(5)

Other cited types(4)

Catalog

Related

[1]	FENG Xue, DAI Xiaojie, FAN Jiangtao, CHEN Pimao. Seasonal variation of fishery resources in Wailingding marine ranching and adjacent waters[J]. South China Fisheries Science, 2023, 19(5): 32-38. DOI: 10.12131/20220308
[2]	JIA Chunyan, WANG Ke, LI Huifeng, GAO Lei, YANG Hao, LIU Shaoping, CHEN Daqing, DUAN Xinbin. Spatial distribution and density changes of fish resources in East Dongting Lake during early fishing ban period[J]. South China Fisheries Science, 2022, 18(3): 48-56. DOI: 10.12131/20210148
[3]	WU Zhi, LI Xinhui, LI Jie, CHEN Fangcan, ZHU Shuli. Acoustic survey of fish resources in Yantan Reservoir in the Red River[J]. South China Fisheries Science, 2017, 13(3): 20-25. DOI: 10.3969/j.issn.2095-0780.2017.03.003
[4]	YUAN Huarong, CHEN Pimao, QIN Chuanxin, LI Xiaoguo, ZHOU Yanbo, FENG Xue, YU Jing, SHU Liming, TANG Zhenzhao, TONG Fei. Seasonal variation of fish community structure in Zhelin Bay, the South China Sea[J]. South China Fisheries Science, 2017, 13(2): 26-35. DOI: 10.3969/j.issn.2095-0780.2017.02.004
[5]	LIAO Xiuli, HUANG Honghui, GONG Xiuyu, YU Shaomei. Seasonal variability in mixed layer depth in Nansha area in 2013[J]. South China Fisheries Science, 2015, 11(5): 67-75. DOI: 10.3969/j.issn.2095-0780.2015.05.008
[6]	ZOU Jianwei, CHEN Lifeng, LIN Jiangjin, LU Zhifu, YANG Bohua. Analysis on variation & distribution of center fishing ground for light falling-net in offshore of the South China Sea: based on statistics of fishery surveillance to Guangxi fishing vessels[J]. South China Fisheries Science, 2014, 10(4): 78-84. DOI: 10.3969/j.issn.2095-0780.2014.04.013
[7]	WU Zhi, TAN Xichang, LI Xinhui, TANG Yong. Acoustic monitoring on fish resources in Xijiang section of Pearl River during first closed fishing season[J]. South China Fisheries Science, 2014, 10(3): 24-28. DOI: 10.3969/j.issn.2095-0780.2014.03.004
[8]	MOU Hongmin, YAO Junjie, NI Chaohui, FANG Guizhen, AN Miao, MA Shan. Hydroacoustic survey of fish resource and spatial distribution in Hongfeng Lake[J]. South China Fisheries Science, 2012, 8(4): 62-69. DOI: 10.3969/j.issn.2095-0780.2012.04.010
[9]	SUN Dianrong, LI Yuan, WANG Xuehui. Seasonal changes of species composition and diversity of fishes in coastal waters of Hainan Island, China[J]. South China Fisheries Science, 2012, 8(1): 1-7. DOI: 10.3969/j.issn.2095-0780.2012.01.001
[10]	LIAO Rui, QU You-jun, GOU Xiao-wei. A review: influence of stocking density on fish welfareⅠ.mortality, growth, feeding and stress response[J]. South China Fisheries Science, 2006, 2(6): 76-80.

Untargeted metabolomics analysis of metabolic differences of crayfish (Procambarus clarkii) meat with different diets

Abstract

References

Related Articles

Cited by

Periodical cited type(5)

Other cited types(4)

Catalog

Related

Export File

Citation

Format

Content