Volume 19 Issue 4
Aug.  2023
Turn off MathJax
Article Contents
Abstract
References
Related Articles
LIU Jiaxing, GUO Huayang, ZHU Kecheng, LIU Baosuo, ZHANG Nan, XIAN Lin, ZHANG Dianchang. Effects of cysteine addition to low-fishmeal diets on metabolism of lipid and protein in juvenile Trachinotus ovatus[J]. South China Fisheries Science, 2023, 19(4): 116-125. DOI: 10.12131/20230030
Citation: LIU Jiaxing, GUO Huayang, ZHU Kecheng, LIU Baosuo, ZHANG Nan, XIAN Lin, ZHANG Dianchang. Effects of cysteine addition to low-fishmeal diets on metabolism of lipid and protein in juvenile Trachinotus ovatus[J]. South China Fisheries Science, 2023, 19(4): 116-125. DOI: 10.12131/20230030
shu

Effects of cysteine addition to low-fishmeal diets on metabolism of lipid and protein in juvenile Trachinotus ovatus

  • 1.

    College of Fisheries and Life Science , Dalian Ocean University, Dalian 116023, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 3.

    Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China

More Information
  • Received Date: March 01, 2023
  • Revised Date: March 29, 2023
  • Accepted Date: April 12, 2023
  • Available Online: April 16, 2023
    Graphical Abstract
    • Abstract
  • To investigate the effect of cysteine on the metabolic disorders of juvenile Trachinotus ovatus [(10.05±0.05) g] caused by low fishmeal, we prepared a basal pomfret diet by using plant and animal proteins (Chicken meal, soybean protein concentrate, fermented soybean meal, etc.) as partial substitutes for fishmeal, and then added 0 (Group C0, control group), 0.30% (Group C1), 0.60% (Group C2), 0.90% (Group C3) and 1.20% (Group C4) cysteine to make five isonitrogenous and isoenergetic diets. The results of a 56-day feeding trial show that: 1) Cysteine enhanced the protein synthesis and metabolism ability of T. ovatus by activating the S6K/PI3K/TOR/4E-BP1 pathway. Supplementation with 0.6%−0.9% cysteine up-regulated the mRNA levels of ribosomal protein S6 kinase (S6K), target of rapamycin (TOR), phosphoinositide 3-kinase (PI3K) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) in muscle, increased total protein (TP) in muscle and serum as well as muscle crude protein content, reduced serum ammonia (SA) and urea nitrogen (UN) in muscle and liver, promoting protein deposition in muscle. 2) Supplementation with 0.6%−0.9% cysteine inhibited lipid anabolism by down-regulating the expression level of peroxisome proliferator-activated receptors gamma (PPARγ) in muscle, decreasing the transcript level and enzyme activity level of Acetyl-CoA carboxylase (ACC) and fatty acid synthetase (FAS), while it up-regulated the expression level of peroxisome proliferator activated receptors-alpha (PPARα) in muscle, resulting in high hormone-sensitive lipase (HSL) and carnitine palmitoyl transferase 1 (CPT1) expression with increasing enzyme activity, which in turn promoted β-oxidation of fatty acids in muscle, reducing protein consumption due to catabolism for energy supply. Thus, protein deposition in muscle is promoted.
    • FullText(HTML)
    • References (40)
  • [1]
    李宁宇, 刘利平, 华雪铭, 等. 豆粕影响日本鳗鲡黑仔鳗饲料中发酵豆粕对鱼粉的替代效果: 生长、抗氧化能力以及生化指标[J]. 海洋渔业, 2020, 42(3): 352-364. doi: 10.3969/j.issn.1004-2490.2020.03.011
    [2]
    冯建, 王萍, 何娇娇, 等. 发酵豆粕替代鱼粉对大黄鱼幼鱼生长性能、体成分、血清生化指标及肝脏组织形态的影响[J]. 动物营养学报, 2016, 28(11): 3493-3502. doi: 10.3969/j.issn.1006-267x.2016.11.016
    [3]
    GUIMARÃES I G, PEZZATO L E, BARROS M M. Amino acid availability and protein digestibility of several protein sources for Nile tilapia, Oreochromis niloticus[J]. Aquac Nutr, 2008, 14(5): 396-404. doi: 10.1111/j.1365-2095.2007.00540.x
    [4]
    崔锡帅. 鸡肉粉、黑水虻幼虫粉和乙醇梭菌蛋白替代鱼粉对暗纹东方鲀生长性能、蛋白代谢及相关基因表达的影响[D]. 上海: 上海海洋大学, 2022: 25.
    [5]
    XIE S C, ZHOU Q C, ZHANG X S, et al. Effect of dietary replacement of fish meal with low-gossypol cottonseed protein concentrate on growth performance and expressions of genes related to protein metabolism for swimming crab (Portunus trituberculatus)[J]. Aquaculture, 2022, 549: 737820. doi: 10.1016/j.aquaculture.2021.737820
    [6]
    HE Y F, CHI S Y, TAN B P, et al. dl-Methionine supplementation in a low-fishmeal diet affects the TOR/S6K pathway by stimulating ASCT2 amino acid transporter and insulin-like growth factor-I in the dorsal muscle of juvenile cobia (Rachycentron canadum)[J]. Brit J Nutr, 2019, 122(7): 734-744. doi: 10.1017/S0007114519001648
    [7]
    de MOURA L B, DIÓGENES A F, CAMPELO D A V, et al. Nutrient digestibility, digestive enzymes activity, bile drainage alterations and plasma metabolites of meagre (Argyrosomus regius) feed high plant protein diets supplemented with taurine and methionine[J]. Aquaculture, 2019, 511(15): 734231.
    [8]
    LI S H, LUO X, LIAO Z B, et al. Additional supplementation of sulfur-containing amino acids in the diets improves the intestinal health of turbot fed high-lipid diets[J]. Fish Shellfish Immunol, 2022, 130: 368-379. doi: 10.1016/j.fsi.2022.09.015
    [9]
    RICHARD N, COLEN R, ARAGÃO C. Supplementing taurine to plant-based diets improves lipid digestive capacity and amino acid retention of Senegalese sole (Solea senegalensis) juveniles[J]. Aquaculture, 2017, 468(1): 94-101.
    [10]
    ESPE M, RUOHONEN K, EL-MOWAFI A. Effect of taurine supplementation on the metabolism and body lipid-to-protein ratio in juvenile Atlantic salmon (Salmo salar)[J]. Aquac Res, 2012, 43(3): 349-360. doi: 10.1111/j.1365-2109.2011.02837.x
    [11]
    LIU J X, ZHU K C, GUO H Y, et al. Effects of cysteine addition to low-fishmeal diets on the growth, anti-oxidative stress, intestine immunity, and Streptococcus agalactiae resistance in juvenile golden pompano (Trachinotus ovatus)[J]. Front Immunol, 2022, 13: 1066936. doi: 10.3389/fimmu.2022.1066936
    [12]
    ELAHI U, WANG J, MA Y B, et al. The response of broiler chickens to dietary soybean meal reduction with glycine and cysteine inclusion at marginal sulfur amino acids (SAA) deficiency[J]. Animals, 2020, 10(9): 1686. doi: 10.3390/ani10091686
    [13]
    CANDEBAT C L, STEPHENS F, BOOTH M A, et al. Adequate levels of dietary sulphur amino acids impart improved liver and gut health in juvenile yellowtail kingfish (Seriola lalandi)[J]. Brit J Nutr, 2022, 129(8): 1289-1312.
    [14]
    NORDRUM S, KROGDAHL Å, RØSJØ C, et al. Effects of methionine, cysteine and medium chain triglycerides on nutrient digestibility, absorption of amino acids along the intestinal tract and nutrient retention in Atlantic salmon (Salmo salar L.) under pair-feeding regime[J]. Aquaculture, 2000, 186(3): 341-360.
    [15]
    GAO J, LIU M J, GUO H Y, et al. ROS Induced by Streptococcus agalactiae activate inflammatory responses via the TNF-α/NF-κB signaling pathway in golden pompano Trachinotus ovatus (Linnaeus, 1758)[J]. Antioxidants, 2022, 11(9): 1809. doi: 10.3390/antiox11091809
    [16]
    LIU J X, GUO H Y, ZHU K C, et al. Effects of exogenous taurine supplementation on the growth, antioxidant capacity, intestine immunity, and resistance against Streptococcus agalactiae in juvenile golden pompano (Trachinotus ovatus) fed with a low-fishmeal diet[J]. Front Immunol, 2022, 13: 1036821. doi: 10.3389/fimmu.2022.1036821
    [17]
    LIU M J, GUO H Y, ZHU K C, et al. Effects of acute ammonia exposure and recovery on the antioxidant response and expression of genes in the Nrf2-Keap1 signaling pathway in the juvenile golden pompano (Trachinotus ovatus)[J]. Aquat Toxicol, 2021, 240: 105969. doi: 10.1016/j.aquatox.2021.105969
    [18]
    LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method[J]. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262
    [19]
    QIN Y W, HE C Q, GENG H Y, et al. Muscle nutritive metabolism changes after dietary fishmeal replaced by cottonseed meal in golden pompano (Trachinotus ovatus)[J]. Metabolites, 2022, 12(7): 576. doi: 10.3390/metabo12070576
    [20]
    ZHOU C P, HUANG Z, LIN H Z, et al. Effects of dietary leucine on glucose metabolism, lipogenesis and insulin pathway in juvenile golden pompano Trachinotus ovatus[J]. Aquac Rep, 2021, 19: 100626. doi: 10.1016/j.aqrep.2021.100626
    [21]
    LIU M J, GUO H Y, LIU B, et al. Gill oxidative damage caused by acute ammonia stress was reduced through the HIF-1α/NF-κb signaling pathway in golden pompano (Trachinotus ovatus)[J]. Ecotox Environ Safe, 2021, 222: 112504. doi: 10.1016/j.ecoenv.2021.112504
    [22]
    MÉTAYER S, SEILIEZ I, COLLIN A, et al. Mechanisms through which sulfur amino acids control protein metabolism and oxidative status[J]. J Nutr Biochem, 2008, 19(4): 207-215. doi: 10.1016/j.jnutbio.2007.05.006
    [23]
    ZHANG H, LUO Y, LU D L, et al. Diacylglycerol oil reduces fat accumulation and increases protein content by inducing lipid catabolism and protein metabolism in Nile tilapia (Oreochromis niloticus)[J]. Aquaculture, 2019, 510: 90-99. doi: 10.1016/j.aquaculture.2019.05.035
    [24]
    TONG S L, WANG L, KALHORO H, et al. Effects of supplementing taurine in all-plant protein diets on growth performance, serum parameters, and cholesterol 7α-hydroxylase gene expression in black sea bream, Acanthopagrus schlegelii[J]. J World Aquac Soc, 2020, 51(4): 990-1001. doi: 10.1111/jwas.12611
    [25]
    EL DALY E S. Protective effect of cysteine and vitamin E, Crocus sativus and Nigella sativa extracts on cisplatin-induced toxicity in rats[J]. J Pharm Belg, 1998, 53(2): 87-93.
    [26]
    DONATO D C Z, SAKOMURA N K, SILVA E P, et al. Manipulation of dietary methionine+cysteine and threonine in broilers significantly decreases environmental nitrogen excretion[J]. Animal, 2016, 10(6): 903-910. doi: 10.1017/S175173111500289X
    [27]
    周乐, 常晨城, 王宇, 等. PI3K/Akt/mTOR信号通路调控牛细胞生长作用的研究进展[J]. 饲料研究, 2022, 45(9): 138-142.
    [28]
    徐稳. 日粮补充亮氨酸调控IUGR仔猪蛋白质代谢及胰岛素信号通路相关基因的机制研究[D]. 南京: 南京农业大学, 2017: 69.
    [29]
    冯文荣, 冯伟, 张明胤, 等. 中华绒螯蟹4E-BP1基因的克隆、表达特征及其在蜕壳中的作用[J]. 中国水产科学, 2022, 29(12): 1714-1727.
    [30]
    TIAN S J, WU Y, YUAN J, et al. Replacement of dietary fishmeal by cottonseed protein concentrate on growth performance, feed utilization and protein metabolism of large yellow croaker Larimichthys crocea[J]. Aquac Rep, 2022, 26: 101313.
    [31]
    HAO Q, WANG L L, ZHANG M H, et al. Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway[J]. Brit J Nutr, 2021, 128(10): 1-12.
    [32]
    SHI X C, JIN A, SUN J, et al. The protein-sparing effect of α-lipoic acid in juvenile grass carp, Ctenopharyngodon idellus: effects on lipolysis, fatty acid β-oxidation and protein synthesis[J]. Brit J Nutr, 2018, 120(9): 977-987. doi: 10.1017/S000711451800226X
    [33]
    WOLBER F M, MCGRATH M, JACKSON F, et al. Cysteic acid in dietary keratin is metabolized to glutathione and liver taurine in a rat model of human digestion[J]. Nutrients, 2016, 8(2): 104. doi: 10.3390/nu8020104
    [34]
    LIU X, DENG H Y, XU Q Q, et al. Effects of tea tree essential oil supplementation in low fish meal diet on growth, lipid metabolism, anti-oxidant capacity and immunity of largemouth bass (Micropterus salmoides)[J]. Aquac Rep, 2022, 27: 101380. doi: 10.1016/j.aqrep.2022.101380
    [35]
    史晓晨. 硫辛酸对草鱼脂质代谢、蛋白质代谢及其抗氧化能力影响的研究[D]. 咸阳: 西北林科技大学, 2018: 45.
    [36]
    张幸开. 维生素A对肉牛肌内脂肪沉积及ACC/HSL、PPARγ基因表达的影响[D]. 咸阳: 西北农林科技大学, 2005: 35-45.
    [37]
    WANG X X, BAI F K, NIU X J, et al. The lipid-lowering effect of dietary taurine in orange-spotted groupers (Epinephelus coioides) involves both bile acids and lipid metabolism[J]. Front Mar Sci, 2022, 9: 859428. doi: 10.3389/fmars.2022.859428
    [38]
    HAJ-YASEIN N N, BERG O, JERNERÉN F, et al. Cysteine deprivation prevents induction of peroxisome proliferator-activated receptor gamma-2 and adipose differentiation of 3T3-L1 cells[J]. BBA-Mol Cell Biol L, 2017, 1862(6): 623-635.
    [39]
    郭亚男, 杨茹艳, 张芷毓, 等. Mstn基因干扰通过上调基因Cpt1b促进肌间脂肪的β氧化[J]. 生物工程学报, 2022, 38(8): 3076-3089.
    [40]
    SHEN J, SUN B F, YU C, et al. Choline and methionine regulate lipid metabolism via the AMPK signaling pathway in hepatocytes exposed to high concentrations of nonesterified fatty acids[J]. J Cell Biochem, 2020, 121(8/9): 3667-3678.
    • Related Articles

  • Related Articles

    [1]WANG Shaomin, MA Zhenhua, HU Jing, WANG Wenfei, MAO Fukao, BAI Zemin, WANG Lixian. Hydrodynamic performance of a square-type submersible net cage under combined wind, wave and current conditions[J]. South China Fisheries Science, 2025, 21(2): 1-13. DOI: 10.12131/20240245
    [2]FU Zhiguo, DING Guolin, DUAN Jinghui, YAO Yunpeng, WANG Lin. Calculation method of hydrodynamic load factors for aquaculture platform netting[J]. South China Fisheries Science. DOI: 10.12131/20240279
    [3]JIANG Shuxia, KONG Xianghong, HUANG Xiaoshuang, YE Xuchang, CAO Daomei. Study on hydrodynamic characteristics and flow field visualization of multi-blade controllable otter board based on CFD[J]. South China Fisheries Science, 2024, 20(5): 136-148. DOI: 10.12131/20240095
    [4]LIU Jingbin, TANG Hao, XU Liuxiong, SUN Qiuyang, LIU Wei, YIN Liqiang, ZHANG Feng. Evaluation of scale effect on hydrodynamic force of V-shaped otter board based on CFD[J]. South China Fisheries Science, 2022, 18(5): 128-137. DOI: 10.12131/20210355
    [5]HAO Yuxin, WAN Rong, ZHOU Cheng, YE Xuchang, GUAN Qinglong, ZHANG Xiaoxian. Hydrodynamic performance of Argentine shortfin squid (Illex argentinus) bottom trawl[J]. South China Fisheries Science, 2022, 18(5): 118-127. DOI: 10.12131/20210343
    [6]SUI Liuyang, HUANG Xiaohua, LIU Haiyang, HU Yu, YUAN Taiping, WANG Shaomin, TAO Qiyou. Effects of mooring pattern on dynamic characteristics of a deep-water aquaculture cage[J]. South China Fisheries Science, 2021, 17(4): 98-108. DOI: 10.12131/20210049
    [7]WANG Shaomin, YUAN Taiping, YANG Xieqiu, TAO Qiyou, SHEN Wei, HU Yu, HUANG Xiaohua. Frequency domain analysis of hydrodynamic characteristics of mariculture ship with truss and plate frame hybrid structure[J]. South China Fisheries Science, 2021, 17(4): 82-90. DOI: 10.12131/20210021
    [8]LI Jie, YAN Lei, YANG Bingzhong, ZHANG Peng. Numerical simulation on untrammeled settlement process of falling-net[J]. South China Fisheries Science, 2017, 13(4): 105-114. DOI: 10.3969/j.issn.2095-0780.2017.04.013
    [9]LIU Jian, HUANG Hongliang, WU Yue, RAO Xin, LI Lingzhi, CHEN Shuai, YANG Jialiang, ZHOU Bin. Model test of hydrodynamic characteristics of two types of vertical cambered slotted otter boards[J]. South China Fisheries Science, 2015, 11(1): 68-74. DOI: 10.3969/j.issn.2095-0780.2015.01.010
    [10]LIU Lili, WAN Rong, WANG Xijie, WANG Sijie, WANG Yunzhong. Analysis of effects of fishery stock enhancement based on system dynamics model[J]. South China Fisheries Science, 2012, 8(1): 16-23. DOI: 10.3969/j.issn.2095-0780.2012.01.003

  • Cited by

    Periodical cited type(1)

    1. 李明. 七轴浇铸机器人的运动学建模和仿真技术. 现代制造技术与装备. 2024(02): 215-217 .

    Other cited types(3)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(4)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return