Volume 18 Issue 5
Oct.  2022
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SU Wenxiao, DENG Yiqin, ZANG Shujun, WANG Qian, LIN Ziyang, FENG Juan. Effects of cbpD gene on virulence and related biological characteristics of Vibrio alginolyticus[J]. South China Fisheries Science, 2022, 18(5): 81-90. DOI: 10.12131/20220025
Citation: SU Wenxiao, DENG Yiqin, ZANG Shujun, WANG Qian, LIN Ziyang, FENG Juan. Effects of cbpD gene on virulence and related biological characteristics of Vibrio alginolyticus[J]. South China Fisheries Science, 2022, 18(5): 81-90. DOI: 10.12131/20220025
shu

Effects of cbpD gene on virulence and related biological characteristics of Vibrio alginolyticus

  • 1.

    Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resource Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 3.

    Tianjin Agricultural University, Tianjin 300392, China

  • 4.

    Ocean Collage, Hainan University, Haikou 570228, China

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  • Received Date: January 23, 2022
  • Revised Date: February 28, 2022
  • Accepted Date: March 10, 2022
  • Available Online: March 25, 2022
    Graphical Abstract
    • Abstract
  • In order to study the effect of the chitin binding protein D (CbpD) on the virulence and related biological characteristics of Vibrio alginolyticus, we constructed the cbpD gene deletion mutant strain of V. alginolyticus ZJ-T by homologous recombination technique. Then we compared the virulence to Danio rerio and other related physiological processes between the wild type strain and the cbpD gene mutant strain, including the growth ability, motility, extracellular protease secretion activity, hemolytic activity and antibiotic sensitivity, biofilm formation, hydrogen peroxide and copper ion resistance as well as ion absorption capacity. The results show that due to the absence of cbpD gene, the swimming motility, swarming motility and extracellular protease activity all reduced, while the resistance to copper ion of the mutant strain strengthened, and its virulence toD. rerio was weakened. However, the deletion of cbpD gene did not affect the growth of the strain in nutrient-rich medium, biofilm formation, hemolytic activity, stress response to H2O2, uptake and utilization of iron and sensitivity to most antibiotics. In conclusion, the results indicate that cbpD may promote the virulence by positively regulating the motility, extracellular protease secretion activity of V. alginolyticus.
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    • References (40)
  • [1]
    REN C, HU C, JIANG X, et al. Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems[J]. Mol Cell Probe, 2013, 27(3): 164-168.
    [2]
    LI X, ZHANG C, WEI F, et al. Bactericidal activity of a holin-endolysin system derived from Vibrio alginolyticus phage HH109[J]. Microb Pathogenesis, 2021, 159: 105135. doi: 10.1016/j.micpath.2021.105135
    [3]
    ZUO Y, ZHAO L, XU X, et al. Mechanisms underlying the virulence regulation of new Vibrio alginolyticus ncRNA Vvrr1 with a comparative proteomic analysis[J]. Emerg Microbes Infec, 2019, 8(1): 1604-1618. doi: 10.1080/22221751.2019.1687261
    [4]
    MOHAMAD N, ROSELI F A M, AZMAI M N A, et al. Natural concurrent infection of Vibrio harveyi and V. alginolyticus in cultured hybrid groupers in Malaysia[J]. J Aquat Anim Health, 2019, 31(1): 88-96. doi: 10.1002/aah.10055
    [5]
    YANG B, ZHAI S, LI X, et al. Identification of Vibrio alginolyticus as a causative pathogen associated with mass summer mortality of the Pacific oyster (Crassostrea gigas) in China[J]. Aquaculture, 2021, 535: 736363. doi: 10.1016/j.aquaculture.2021.736363
    [6]
    FAHMY N M, HAMED E sayed A E. Isolation and characterization of Vibrio alginolyticus strain HAT3 causing skin ulceration disease in cultured sea cucumber Holothuria atra (Jaeger, 1833)[J]. Egypt J Aquat Res, 2022, 48(1): 75-81.
    [7]
    ZHAO Z, LIU J, DENG Y, et al. The Vibrio alginolyticus T3SS effectors, Val1686 and Val1680, induce cell rounding, apoptosis and lysis of fish epithelial cells[J]. Virulence, 2018, 9(1): 318-330. doi: 10.1080/21505594.2017.1414134
    [8]
    王俊霖, 招茵, 苏茵茵, 等. 溶藻弧菌T3SS exsD基因敲除突变株构建及其表型特征[J]. 广东海洋大学学报, 2021, 41(5): 35-43. doi: 10.3969/j.issn.1673-9159.2021.05.005
    [9]
    BUNPA S, CHAICHANA N, TENG J L L, et al. Outer membrane protein A (OmpA) is a potential virulence factor of Vibrio alginolyticus strains isolated from diseased fish[J]. J Fish Dis, 2020, 43(2): 275-284. doi: 10.1111/jfd.13120
    [10]
    CAI S, CHENG H, PANG H, et al. AcfA is an essential regulator for pathogenesis of fish pathogen Vibrio alginolyticus[J]. Vet Microbiol, 2018, 213: 35-41. doi: 10.1016/j.vetmic.2017.11.016
    [11]
    HUANG L, GUO L, XU X, et al. The role of RpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses[J]. J Fish Dis, 2019, 42(5): 703-712. doi: 10.1111/jfd.12972
    [12]
    GAVIARD C, COSETTE P, JOUENNE T, et al. LasB and CbpD virulence factors of Pseudomonas aeruginosa carry multiple post-translational modifications on their lysine residues[J]. J Proteome Res, 2019, 18(3): 923-933.
    [13]
    王晓辉. 海洋细菌DL-6几丁质酶和几丁质结合蛋白的生化性质与功能研究[D]. 大连: 大连理工大学, 2016: 14.
    [14]
    ASKARIAN F, UCHIYAMA S, MASSON H, et al. The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection[J]. Nat Commun, 2021, 12(1): 1230. doi: 10.1038/s41467-021-21473-0
    [15]
    HUANG X, CHEN C, REN C, et al. Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51[J]. Biofouling, 2018, 34(1): 1-14. doi: 10.1080/08927014.2017.1400020
    [16]
    le ROUX F, BINESSE J, SAULNIER D, et al. Construction of a Vibrio splendidus mutant lacking the metalloprotease gene vsm by use of a novel counterselectable suicide vector[J]. Appl Environ Microb, 2007, 73(3): 777-784. doi: 10.1128/AEM.02147-06
    [17]
    NGUYEN A N, DISCONZI E, CHARRIÈRE G M, et al. csrb gene duplication drives the evolution of redundant regulatory pathways controlling expression of the major toxic secreted metalloproteases in Vibrio tasmaniensis LGP32[J]. MSphere, 2018, 3(6): e00582-18.
    [18]
    VAL M E, SKOVGAARD O, DUCOS-GALAND M, et al. Genome engineering in Vibrio cholerae: a feasible approach to address biological issues[J]. PLOS Genet, 2012, 8(1): e1002472. doi: 10.1371/journal.pgen.1002472
    [19]
    DENG Y, CHEN C, ZHAO Z, et al. The RNA chaperone Hfq is involved in colony morphology, nutrient utilization and oxidative and envelope stress response in Vibrio alginolyticus[J]. PLOS ONE, 2016, 11(9): e0163689. doi: 10.1371/journal.pone.0163689
    [20]
    VAZQUEZ S, MERINO L, MACCORMACK W, et al. Protease-producing psychrotrophic bacteria isolated from Antarctica[J]. Polar Biol, 1995, 15(2): 131-135.
    [21]
    邓益琴, 陈偿, 苏友禄, 等. 溶藻弧菌ZJ-T小RNA srvg23535基因突变株的构建及其功能初探[J]. 微生物学通报, 2019, 46(4): 829-841.
    [22]
    SPAN E A, MARIETTA M A. The framework of polysaccharide monooxygenase structure and chemistry[J]. Curr Opin Struc Biol, 2015, 35: 93-99. doi: 10.1016/j.sbi.2015.10.002
    [23]
    BHOWMICK R, GHOSAL A, DAS B, et al. Intestinal adherence of Vibrio cholerae involves a coordinated interaction between colonization factor GbpA and mucin[J]. Infect Immun, 2008, 76(11): 4968-4977. doi: 10.1128/IAI.01615-07
    [24]
    CHAUDHURI S, BRUNO J C, ALONZO F, et al. Contribution of chitinases to Listeria monocytogenes pathogenesis[J]. Appl Environ Microb, 2010, 76(21): 7302-7305. doi: 10.1128/AEM.01338-10
    [25]
    FREDERIKSEN R F, PASPALIARI D K, LARSEN T, et al. Bacterial chitinases and chitin-binding proteins as virulence factors[J]. Microbiology, 2013, 159(Pt5): 833-847.
    [26]
    JOSENHANS C, SUERBAUM S. The role of motility as a virulence factor in bacteria[J]. Int J Med Microbiol, 2002, 291(8): 605-614. doi: 10.1078/1438-4221-00173
    [27]
    ZHOU S, TU X, PANG H, et al. A T3SS regulator mutant of Vibrio alginolyticus affects antibiotic susceptibilities and provides significant protection to Danio rerio as a live attenuated vaccine[J]. Front Cell Infect Mi, 2020, 10: 183. doi: 10.3389/fcimb.2020.00183
    [28]
    CAO X, WANG Q, LIU Q, et al. Identification of a LuxO-regulated extracellular protein Pep and its roles in motility in Vibrio alginolyticus[J]. Microb Pathogenesis, 2011, 50(2): 123-131. doi: 10.1016/j.micpath.2010.12.003
    [29]
    ZHOU Z, PANG H, DING Y, et al. VscO, a putative T3SS chaperone escort of Vibrio alginolyticus, contributes to virulence in fish and is a target for vaccine development[J]. Fish Shellfish Immunol, 2013, 35(5): 1523-1531. doi: 10.1016/j.fsi.2013.08.017
    [30]
    WATNICK P I, LAURIANO C M, KLOSE K E, et al. The absence of a flagellum leads to altered colony morphology, biofilm development and virulence in Vibrio cholerae O139[J]. Mol Microbiol, 2001, 39(2): 223-235. doi: 10.1046/j.1365-2958.2001.02195.x
    [31]
    李莹玉, 贺小贤, 蒋合阳, 等. 组氨酸激酶BaeS对溶藻弧菌毒力因子和环境应激的作用[J]. 陕西科技大学学报, 2022, 40(1): 57-64.
    [32]
    KIM I H, KIM S Y, PARK N Y, et al. Cyclo-(L-Phe-L-Pro), a quorum-sensing signal of Vibrio vulnificus, induces expression of hydroperoxidase through a ToxR-LeuO-HU-RpoS signaling pathway to confer resistance against oxidative stress[J]. Infect Immun, 2018, 86(9): e00932-17.
    [33]
    KARLIN K D. Metalloenzymes, structural motifs, and inorganic models[J]. Science, 1993, 261(5122): 701-708. doi: 10.1126/science.7688141
    [34]
    HODGKINSON V, PETRIS M J. Copper homeostasis at the host-pathogen interface[J]. J Biol Chem, 2012, 287(17): 13549-13555. doi: 10.1074/jbc.R111.316406
    [35]
    VANHOVE A S, RUBIO T P, NGUYEN A N, et al. Copper homeostasis at the host Vibrio interface: lessons from intracellular Vibrio transcriptomics[J]. Environ Microbiol, 2016, 18(3): 875-888. doi: 10.1111/1462-2920.13083
    [36]
    KONG W, HUANG L, SU Y, et al. Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis[J]. Antonie Van Leeuwenhoek, 2015, 107(5): 1197-1206. doi: 10.1007/s10482-015-0411-9
    [37]
    AFFANDI T, MCEVOY M M. Mechanism of metal ion-induced activation of a two-component sensor kinase[J]. Biochem J, 2019, 476(1): 115-135. doi: 10.1042/BCJ20180577
    [38]
    BURRIDGE L, WEIS J S, CABELLO F, et al. Chemical use in salmon aquaculture: a review of current practices and possible environmental effects[J]. Aquaculture, 2010, 306(1/2/3/4): 7-23.
    [39]
    RATTANAMA P, THOMPSON J R, KONGKERD N, et al. Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi[J]. PLOS ONE, 2012, 7(2): e32523. doi: 10.1371/journal.pone.0032523
    [40]
    HALL-STOODLEY L, STOODLEY P. Biofilm formation and dispersal and the transmission of human pathogens[J]. Trends Microbiol, 2005, 13(1): 7-10. doi: 10.1016/j.tim.2004.11.004
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