Volume 18 Issue 6
Dec.  2022
Turn off MathJax
Article Contents
Abstract
References
MA Tengfei, WEN Bin, LIU Xin, MIAO Lin, GAO Jianzhong, CHEN Zaizhong. Molecular cloning and characterization in immune response of two C-type lectin genes (SaCD302 andSaMBL) from Symphysodon aequifasciatus[J]. South China Fisheries Science, 2022, 18(6): 35-43. DOI: 10.12131/20220018
Citation: MA Tengfei, WEN Bin, LIU Xin, MIAO Lin, GAO Jianzhong, CHEN Zaizhong. Molecular cloning and characterization in immune response of two C-type lectin genes (SaCD302 andSaMBL) from Symphysodon aequifasciatus[J]. South China Fisheries Science, 2022, 18(6): 35-43. DOI: 10.12131/20220018
shu

Molecular cloning and characterization in immune response of two C-type lectin genes (SaCD302 andSaMBL) from Symphysodon aequifasciatus

  • 1.

    Shanghai Ocean University/Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education/Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai, 201306, China

  • 2.

    Shanghai Ocean University/Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai 201306, China

More Information
  • Received Date: January 16, 2022
  • Revised Date: March 30, 2022
  • Accepted Date: May 06, 2022
  • Available Online: May 12, 2022
    Graphical Abstract
    • Abstract
  • Symphysodon aequifasciatus has a unique parental rearing behavior. In the previous study, two C-type lectins (CTLs) were found to be highly expressed in the skin of parent fish, but with different expression patterns. In order to investigate the difference in the potential immune functions of the specific CTLs, we cloned two CTL genes (SaCD302 and SaMBL), then simulated and analyzed their immune esponse patterns under pathological infection. The results show that the ORF region ofSaCD302 and SaMBL were 741 and 795 bp in length, encoding 246 and 264 amino acids (aa), respectively. The SaCD302 sequence contained a signal peptide (aa 1–29), a C-type lectin-like domain (CTLD) (aa 32–169) and a transmembrane domain (aa 184–206). Two low complexity regions (aa 32–92 and aa 104–121) and a CTLD (aa 137–263) were expressed in SaMBL sequence. Moreover, SaCD302 and SaMBL shared the highest similarity with that of Archocentrus centrarchus. SaCD302 was highly expressed in the head kidney, followed by the liver and gill. Compared with the intestine, liver, spleen and gonads, the skin had the higher expression of SaMBL. After Aeromonas hydrophila infection, SaCD302 expression was significantly upregulated in the spleen, head kidney, intestine and skin, whileSaMBL was only upregulated in the head kidney, intestine and skin, and these two genes were expressed in different temporal manners, indicating that they might play different roles in the immune defense of S. aequifasciatus.
    • FullText(HTML)
    • References (31)
  • [1]
    刘晓东, 陈再忠. 七彩神仙鱼皮肤色素细胞观察及类胡萝卜素组分分析[J]. 上海水产大学学报, 2008(3): 339-343.
    [2]
    何文辉, 张美琼, 鲍宝龙. 七彩神仙鱼人工繁殖主要水质条件的探讨[J]. 上海水产大学学报, 2001(1): 22-25.
    [3]
    鲁凯, 高建忠, 温彬, 等. 黑格尔 (Symphysodon discus Heckel) ×松石七彩神仙鱼 (Symphysodon discus Turquoise) 杂交后代的遗传变异[J]. 基因组学与应用生物学, 2019, 38(11): 4951-4960.
    [4]
    SATOH S, TANOUE H, MOHRI M. Costs and benefits of biparental mucus provisioning in discus fish (Symphysodon aequifasciatus)[J]. Ichthyol Res, 2018, 65(4): 510-514. doi: 10.1007/s10228-018-0636-5
    [5]
    SCHUTZ M, BARLOW G W. Young of the Midas cichlid get biologically active nonnutrients by eating mucus from the surface of their parents[J]. Fish Physiol Biochem, 1997, 16(1): 11-18. doi: 10.1007/BF00004536
    [6]
    CHONG K, JOSHI S, JIN L T, et al. Proteomics profiling of epidermal mucus secretion of a cichlid (Symphysodon aequifasciata) demonstrating parental care behavior[J]. Proteomics, 2010, 6(7): 2251-2258.
    [7]
    DRICKAMER K. C-type lectin-like domains[J]. Curr Opin Struct Biol, 1999, 9(5): 585-590. doi: 10.1016/S0959-440X(99)00009-3
    [8]
    DRICKAMER K. Evolution of Ca2+-dependent animal lectins[J]. Prog Nucl Acid Res Mol Biol, 1993, 45: 207-232.
    [9]
    谢建辉, 顾建新. C型凝集素[J]. 生命科学, 2011, 23(6): 555-562.
    [10]
    OSORIO F, REIS E S C. Myeloid C-type lectin receptors in pathogen recognition and host defense[J]. Immunity, 2011, 34(5): 651-664. doi: 10.1016/j.immuni.2011.05.001
    [11]
    VASTA G R, AHMED H, ODOM E W. Structural and functional diversity of lectin repertoires in invertebrates, protochordates and ectothermic vertebrates[J]. Curr Opin Struct Biol, 2004, 14(5): 617-630. doi: 10.1016/j.sbi.2004.09.008
    [12]
    ZELENSKY A N, GREADY J E. The C-type lectin-like domain superfamily[J]. FEBS J, 2010, 272(24): 6179-6217.
    [13]
    KERRIGAN A M, BROWN G D. C-type lectins and phagocytosis[J]. Immunobiology, 2009, 214(7): 562-575. doi: 10.1016/j.imbio.2008.11.003
    [14]
    CHEN S X, MA H L, SHI Y H, et al. Molecular and functional characterization of a novel CD302 gene from ayu (Plecoglossus altivelis)[J]. Fish Shellfish Immunol, 2016, 55: 140-148. doi: 10.1016/j.fsi.2016.05.022
    [15]
    KILPATRICK D C. Mannan-binding lectin: clinical significance and applications[J]. Biochim Biophys Acta Gen Subj, 2002, 1572(2/3): 401-413. doi: 10.1016/S0304-4165(02)00321-5
    [16]
    STUART L M, TAKAHASHI K, SHI L, et al. Mannose-binding lectin-deficient mice display defective apoptotic cell clearance but no autoimmune phenotype[J]. J Immunol, 2005, 174(6): 3220-3226. doi: 10.4049/jimmunol.174.6.3220
    [17]
    赵中利. MBL2基因与奶牛乳腺炎抗性研究[D]. 长春: 吉林大学, 2012: 6-7.
    [18]
    KHARDORI N, FAINSTEIN V. Aeromonas and Plesiomonas as etiological agents[J]. Annu Rev Microbiol, 1988, 42(1): 395-419. doi: 10.1146/annurev.mi.42.100188.002143
    [19]
    HOSSAIN S, HEO G J. Ornamental fish: a potential source of pathogenic and multidrug-resistant motile Aeromonas spp.[J]. Lett Appl Microbiol, 2021, 72(1): 2-12. doi: 10.1111/lam.13373
    [20]
    英娜, 王元, 秦搏, 等. 七彩神仙鱼肠炎病原菌的分离鉴定及药敏试验[J]. 水产科技情报, 2020, 47(5): 245-248.
    [21]
    ABD EL-GHANY N A, EL-KHATIB N R, SALAMA S S A. Causes of mortality in discus fish (Symphysodon) and trials for treatment[J]. Egypt J Aquac, 2014, 4: 1-12.
    [22]
    魏域玲, 温彬, 高建忠, 等. 七彩神仙鱼(Symphysodon haraldi) OTX2基因克隆及其表达分析[J]. 淡水渔业, 2021, 51(3): 13-19. doi: 10.3969/j.issn.1000-6907.2021.03.002
    [23]
    ROBINSON M J, SANCHO D, SLACK E C, et al. Myeloid C-type lectins in innate immunity[J]. Nat Immunol, 2006, 7(12): 1258-1265. doi: 10.1038/ni1417
    [24]
    郝少彦, 尹丝雨, 龚晨, 等. 一种凡纳滨对虾新的C型凝集素基因 (LvLc2) 的克隆及免疫应答特征[J]. 水产学报, 2021, 45(6): 948-957.
    [25]
    毕彩红. 松江鲈 (Trachidermus fasciatus) 两种C型凝集素的基因克隆表达与功能分析[D]. 济南: 山东大学, 2015: 54-55.
    [26]
    张艺榕, 李长红, 陈炯. 花鲈C型凝集素受体CD302基因的分子鉴定及其在哈维氏弧菌感染下的表达[J]. 农业生物技术学报, 2021, 29(3): 424-434.
    [27]
    HAO L, SUN L. Comparative analysis of the expression patterns of eight suppressors of cytokine signaling in tongue sole, Cynoglossus semilaevis[J]. Fish Shellfish Immunol, 2016, 55: 595-601. doi: 10.1016/j.fsi.2016.06.034
    [28]
    KANIA P W, SORENSEN R R, KOCH C, et al. Evolutionary conservation of mannan-binding lectin (MBL) in bony fish: identification, characterization and expression analysis of three bona fide collectin homologues of MBL in the rainbow trout (Onchorhynchus mykiss)[J]. Fish Shellfish Immunol, 2010, 29(6): 910-920. doi: 10.1016/j.fsi.2010.07.020
    [29]
    党云飞. 草鱼抗菌免疫基因筛选及补体凝集素途径基因功能研究[D]. 上海: 上海海洋大学, 2017: 48-50.
    [30]
    HUANG Z H, AIJUN M A, LEI J L. Progress in study on the skin mucus lectin in fish[J]. Zool Res, 2013, 34(6): 674-679.
    [31]
    吕爱军, 胡秀彩, 孙敬锋, 等. 鱼类皮肤免疫应答及蛋白质组学[J]. 水产科学, 2016, 35(3): 302-307.
    • Related Articles

  • Cited by

    Periodical cited type(0)

    Other cited types(1)

Catalog

    Get Citation
    PDF
    XML
    Article views (620) PDF downloads (56) Cited by(1)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return