ZHANG Zhe, GONG Xiuyu, LAN Lili, WANG Xuefeng, MA Shengwei, CHEN Haigang, ZHANG Linbao. Cloning and expression profiling of Cyp1a gene in Lutjanus argentimaculatus under 4-bromodiphenyl ether (BDE-3) and decabromodiphenyl ether (BDE-209) exposure[J]. South China Fisheries Science, 2022, 18(4): 54-64. DOI: 10.12131/20210271
Citation: ZHANG Zhe, GONG Xiuyu, LAN Lili, WANG Xuefeng, MA Shengwei, CHEN Haigang, ZHANG Linbao. Cloning and expression profiling of Cyp1a gene in Lutjanus argentimaculatus under 4-bromodiphenyl ether (BDE-3) and decabromodiphenyl ether (BDE-209) exposure[J]. South China Fisheries Science, 2022, 18(4): 54-64. DOI: 10.12131/20210271

Cloning and expression profiling of Cyp1a gene in Lutjanus argentimaculatus under 4-bromodiphenyl ether (BDE-3) and decabromodiphenyl ether (BDE-209) exposure

More Information
  • Received Date: September 21, 2021
  • Revised Date: November 03, 2021
  • Accepted Date: November 30, 2021
  • Available Online: December 13, 2021
  • Cytochrome P450 enzymes (CYPs) are encoded by P450 genes, in which cytochrome P450 1A (Cyp1a) genes mainly involve in biotransformation and metabolism of numerous xenobiotics. In this study, we cloned the Cyp1a gene from Lutjanus argentimaculatus, and investigated its tissue expression pattern. In addition, we evaluated different concentrations (10, 50 and 250 μg·L−1) of BDE-3 and BDE-209 on Cyp1a gene profile and 7-ethoxyresorufin-O-deethylase (EROD) activity in liver of L. argentimaculatus. The results show that the full length of Cyp1a cDNA was 2 540 bp with 1 566 open reading frame encoding 521 amino acids. The sequence homology of L. argentimaculatus CYP1A was the highest (92.69%) with that of Lateolabrax maculatus. Phylogenetic analysis results indicate that CYP1A was closely aligned with Sander lucioperca protein. Cyp1a transcripts were most abundant in liver, followed by brain and gill, but lowest in muscle. 10 μg·L−1 of BDE-3 and BDE-209 showed no effects on both Cyp1a expression and EROD activity, while high concentrations (50 and 250 μg·L−1) of BDE-3 down-regulated both of them significantly in a concentration-dependent manner on 7th-15th day. In contrast, exposure to 50 and 250 μg·L−1 of BDE-209 resulted in increasing of hepatic Cyp1a level and EROD activity. Moreover, Cyp1a genes levels and EROD activities showed a good correlation. High concentrations of BDE-3 and BDE-209 can affect Cyp1a gene expression in liver of L. argentimaculatus in different manners.
  • [1]
    LU K, SONG Y, ZENG R. The role of cytochrome P450-mediated detoxification in the insect adaptation to xenobiotics[J]. Curr Opin Insect Sci, 2021, 43: 103-107. doi: 10.1016/j.cois.2020.11.004
    [2]
    LAU I C K, FEYEREISEN R, NELSON D R, et al. Analysis and preliminary characterisation of the cytochrome P450 monooxygenases from Frankia sp. EuI1c (Frankia inefficax sp. )[J]. Arch Biochem Biophys, 2019, 669: 11-21. doi: 10.1016/j.abb.2019.05.007
    [3]
    MILLER J C, HOLLATZ A J, SCHULER M A. P450 variations bifurcate the early terpene indole alkaloid pathway in Catharanthus roseus and Camptotheca acuminate[J]. Phytochemistry, 2021, 183: 112626. doi: 10.1016/j.phytochem.2020.112626
    [4]
    YANG T, LI T, FENG X, et al. Multiple cytochrome P450 genes: conferring high levels of permethrin resistance in mosquitoes, Culex quinquefasciatus[J]. Sci Rep, 2021, 11(1): 9041. doi: 10.1038/s41598-021-88121-x
    [5]
    NELSON D R. Cytochrome P450 diversity in the tree of life[J]. Biochim Biophys Acta Proteins Proteom, 2018, 1866(1): 141-154. doi: 10.1016/j.bbapap.2017.05.003
    [6]
    张文领, 牟希东, 胡隐昌, 等. 福寿螺细胞色素P450 基因CYP3192A1 的克隆与表达分析[J]. 南方水产科学, 2017, 13(1): 66-75. doi: 10.3969/j.issn.2095-0780.2017.01.009
    [7]
    HAN J, KIM D, KIM H, et al. Genome-wide identification of 52 cytochrome P450 (CYP) genes in the copepod Tigriopus japonicus and their B[α]P-induced expression patterns[J]. Comp Biochem Physiol D, 2017, 23: 49-57.
    [8]
    高锴, 闫佩, 檀翠玲, 等. 虹鳟鱼鳃及肝脏多种CYP1 基因表达模式作为生物标志物监测海河水污染状况[J]. 环境科学, 2015, 36(10): 3878-3883.
    [9]
    PENG F, HARDY E M, BÉRANGER R, et al. Human exposure to PCBs, PBDEs and bisphenols revealed by hair analysis: a comparison between two adult female populations in China and France[J]. Environ Pollut, 2020, 267: 115425. doi: 10.1016/j.envpol.2020.115425
    [10]
    DA C, WANG R, XIA L, et al. Sediment records of polybrominated diphenyl ethers (PBDEs) in Yangtze River Delta of Yangtze River in China[J]. Mar Pollut Bull, 2021, 160: 111714.
    [11]
    LIU B, SONG N, JIANG T, et al. Polybrominated diphenyl ethers in surface sediments from fishing ports along the coast of Bohai Sea, China[J]. Mar Pollut Bull, 2021, 164: 112037. doi: 10.1016/j.marpolbul.2021.112037
    [12]
    YUAN J, SUN X, CHE S, et al. AhR-mediated CYP1A1 and ROS overexpression are involved in hepatotoxicity of decabromodiphenyl ether (BDE-209)[J]. Toxicol Lett, 2021, 352: 26-33. doi: 10.1016/j.toxlet.2021.09.008
    [13]
    LI Y, MA F, LI Z, et al. Exposure to 4-bromodiphenyl ether during pregnancy blocks testis development in male rat fetuses[J]. Toxicol Lett, 2021, 342: 38-49. doi: 10.1016/j.toxlet.2021.02.004
    [14]
    YAO Y, WANG B, HE Y, et al. Fate of 4-bromodiphenyl ether (BDE3) in soil and the effects of co-existed copper[J]. Environ Pollut, 2020, 261: 114214. doi: 10.1016/j.envpol.2020.114214
    [15]
    李嘉伟, 尹晓宇, 周旖旎, 等. 五溴联苯醚(BDE-99)和羟基五溴联苯醚(5-OH-BDE-99)经由THRβ影响斑马鱼胚胎眼部色素的沉着[J]. 生态毒理学报, 2020, 15(5): 181-188.
    [16]
    THORNTON L M, PATH E M, NYSTROM G S, et al. Embryo-larval BDE-47 exposure causes decreased pathogen resistance in adult male fathead minnows (Pimephales promelas)[J]. Fish Shellfish Immunol, 2018, 80: 80-87. doi: 10.1016/j.fsi.2018.05.059
    [17]
    王余江, 樊琳, 陈创奇, 等. 视黄酸和多溴联苯醚联合暴露对斑马鱼运动行为的影响[J]. 生态毒理学报, 2019, 14(2): 260-267. doi: 10.7524/AJE.1673-5897.20180206002
    [18]
    YANG J, ZHAO H, CHAN K M. Toxic effects of polybrominated diphenyl ethers (BDE 47 and 99) and localization of BDE-99-induced cyp1a mRNA in zebrafish larvae[J]. Toxicol Rep, 2017, 4: 614-624. doi: 10.1016/j.toxrep.2017.11.003
    [19]
    SØFTELAND L, PETERSEN K, STAVRUM A, et al. Hepatic in vitro toxicity assessment of PBDE congeners BDE47, BDE153 and BDE154 in Atlantic salmon (Salmo salar L. )[J]. Aquat Toxicol, 2011, 105(3/4): 246-263.
    [20]
    BOON J P, ZANDEN J J, LEWIS W E, et al. The expression of CYP1A, vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses[J]. Mar Environ Res, 2002, 54(3-5): 719-724. doi: 10.1016/S0141-1136(02)00127-7
    [21]
    MUYOT F B, MAGISTRADO M L, MUYOT M C, et al. Growth performance of the mangrove red snapper (Lutjanus argentimaculatus) in freshwater pond comparing two stocking densities and three feed types[J]. Philippine J Fish, 2021, 28(1): 1-7.
    [22]
    CHEN H, ZHANG Z, ZHANG L, et al. Effects of di-n-butyl phthalate on gills- and liver-specific EROD activities and CYP1A levels in juvenile red snapper (Lutjanus argentimaculatus)[J]. Comp Biochem Physiol C, 2020, 232: 108757.
    [23]
    KUMAR S, STECHER G, TAMURA K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Mol Biol Evol, 2016, 33(7): 1870-1874. doi: 10.1093/molbev/msw054
    [24]
    余铭恩, 郑榕辉, 张玉生. 3种海洋鱼类肝微粒体EROD活性的测定[J]. 生态学报, 2014, 34(19): 5416-5424.
    [25]
    LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262
    [26]
    CHAUBE R, RAWAT A, INBARAJ R M, et al. Cloning and characterization of estrogen hydroxylase (cyp1a1 and cyp1b1) genes in the stinging catfish Heteropneustes fossilis and induction of mRNA expression during final oocyte maturation[J]. Comp Biochem Physiol A, 2021, 253: 110863. doi: 10.1016/j.cbpa.2020.110863
    [27]
    ARUKWE A. Complementary DNA cloning, sequence analysis and differential organ expression of β-naphthoflavone-inducible cytochrome P4501A in Atlantic salmon (Salmo salar)[J]. Comp Biochem Physiol C, 2002, 133(4): 613-624.
    [28]
    WOO S J, CHUNG J K. Cytochrome P450 1 enzymes in black rockfish, Sebastes schlegelii: molecular characterization and expression patterns after exposure to benzo[a]pyrene[J]. Aquat Toxicol, 2020, 226: 105566. doi: 10.1016/j.aquatox.2020.105566
    [29]
    COCCI P, MOSCONI G, PALERMO F A. Effects of 4-nonylphenol on hepatic gene expression of peroxisome proliferator-activated receptors and cytochrome P450 isoforms (CYP1A1 and CYP3A4) in juvenile sole (Solea solea)[J]. Chemosphere, 2013, 93(6): 1176-1181. doi: 10.1016/j.chemosphere.2013.06.058
    [30]
    梁秋芳, 董小燕, 冯平. CYP2D 亚家族基因及其进化机制研究进展[J]. 广西师范大学学报 (自然科学版), 2021, 39(5): 58-63.
    [31]
    BURKIAN V, ZAMARATSKAIA G, SAKALLI S, et al. Tissue-specific expression and activity of cytochrome P450 1A and 3A in rainbow trout (Oncorhynchus mykiss)[J]. Toxicol Lett, 2021, 341: 1-10. doi: 10.1016/j.toxlet.2021.01.011
    [32]
    RUSNI S, SASSA M, TAKEHANA Y, et al. Correlation between cytochrome P450 1A (cyp1a) mRNA expression and ambient phenanthrene and pyrene concentration in Javanese medaka Oryzias javanicus[J]. Fish Sci, 2020, 86: 605-613. doi: 10.1007/s12562-020-01428-y
    [33]
    KIM R, KIM B, HWANG D, et al. Evaluation of biomarker potential of cytochrome P450 1A (CYP1A) gene in the marine medaka, Oryzias melastigma exposed to water-accommodated fractions (WAFs) of Iranian crude oil[J]. Comp Biochem Physiol C, 2013, 157(2): 172-182.
    [34]
    PETRULIS J R, CHEN G, BENN S, et al. Application of the ethoxyresorufin-O-deethylase (EROD) assay to mixtures of halogenated aromatic compounds[J]. Environ Toxicol, 2001, 16(2): 177-184. doi: 10.1002/tox.1022
    [35]
    ROY M A, SANT K E, VENEZIA O L, et al. The emerging contaminant 3, 3'-dichlorobiphenyl (PCB-11) impedes Ahr activation and Cyp1a activity to modify embryotoxicity of Ahr ligands in the zebrafish embryo model (Danio rerio)[J]. Environ Pollut, 2019, 254: 113027. doi: 10.1016/j.envpol.2019.113027
    [36]
    SMITH E M, IFTIKAR F I, HIGGINS S, et al. In vitro inhibition of cytochrome P450-mediated reactions by gemfibrozil, erythromycin, ciprofloxacin and fluoxetine in fish liver microsomes[J]. Aquat Toxicol, 2012, 109: 259-266. doi: 10.1016/j.aquatox.2011.08.022
    [37]
    DAR S A, GORA A H, BHAT I A, et al. Studies of anthelminthic benzimidazole derivatives on cytochrome P450 1A (CYP1A) dependent detoxification mechanism in Labeo rohita[J]. Aquaculture, 2017, 481: 79-84. doi: 10.1016/j.aquaculture.2017.08.015
    [38]
    迟潇, 陈碧娟, 孙雪梅, 等. 基于IBR模型研究BDE-47和BDE-153对半滑舌鳎的毒性效应[J]. 生态毒理学报, 2020, 15(4): 192-202.
    [39]
    VEN L T M V, KUIL T, LEONARDS P E G, et al. A 28-day oral dose toxicity study in Wistar rats enhanced to detect endocrine effects of decabromodiphenyl ether (decaBDE)[J]. Toxicol Lett, 2008, 179(1): 6-14. doi: 10.1016/j.toxlet.2008.03.003
    [40]
    WANG B, WANG H, XIAO D, et al. In vitro effects of brominated flame retardants, selected metals and their mixtures on ethoxyresorufin-O-deethylase activity in Mossambica tilapia liver[J]. Ecotoxicol Environ Saf, 2018, 161: 350-355. doi: 10.1016/j.ecoenv.2018.05.084
    [41]
    黄志斐, 马胜伟, 张喆, 等. BDE3胁迫对翡翠贻贝 (Perna viridis) SOD、MDA和GSH的影响[J]. 南方水产科学, 2012, 8(5): 25-30. doi: 10.3969/j.issn.2095-0780.2012.05.004
    [42]
    XIE Z, LU G, QI P. Effects of BDE-209 and its mixtures with BDE-47 and BDE-99 on multiple biomarkers in Carassius auratus[J]. Environ Toxicol Pharmacol, 2014, 38(2): 554-561. doi: 10.1016/j.etap.2014.08.008
    [43]
    YANG J, ZHU J, CHAN K M. BDE-99, but not BDE-47, is a transient aryl hydrocarbon receptor agonist in zebrafish liver cells[J]. Toxicol Appl Pharmacol, 2016, 305: 203-215. doi: 10.1016/j.taap.2016.06.023
    [44]
    SHARIFIAN S, HOMAEI A, KAMRANI E, et al. New insights on the marine cytochrome P450 enzymes and their biotechnological importance[J]. Int Biol Macromol, 2020, 142: 811-821. doi: 10.1016/j.ijbiomac.2019.10.022
    [45]
    SÁNCHEZ-OCAMPO E M, AZUELA G E, SALAS M S, et al. Alterations in viability and CYP1A1 expression in SH SY5Y cell line by pollutants present in Madín Dam, Mexico[J]. Sci Total Environ, 2020, 719: 137500. doi: 10.1016/j.scitotenv.2020.137500
    [46]
    LI Z, ZHONG L, MU W, et al. Effects of chronic exposure to tributyltin on tissue specific cytochrome P450 1 regulation in juvenile common carp[J]. Xenobiotica, 2016, 46(6): 511-515. doi: 10.3109/00498254.2015.1092618
    [47]
    CAPPELLETTI N, SPERANZA E, TATONE L, et al. Bioaccumulation of dioxin-like PCBs and PBDEs by detritus-feeding fish in the Rio de la Plata estuary, Argentina[J]. Environ Sci Pollut Res Int, 2015, 22(9): 7093-7100. doi: 10.1007/s11356-014-3935-z
    [48]
    KUIPER R V, BERGMAN Å, VOS J G, et al. Some polybrominated diphenyl ether (PBDE) flame retardants with wide environmental distribution inhibit TCDD-induced EROD activity in primary cultured carp (Cyprinus carpio) hepatocytes[J]. Aquat Toxicol, 2004, 68(2): 129-139. doi: 10.1016/j.aquatox.2004.03.005
    [49]
    WHAL M, LAHNI B, GUENTHER R, et al. A technical mixture of 2, 2', 4, 4'-tetrabromo diphenyl ether (BDE47) and brominated furans triggers aryl hydrocarbon receptor (AhR) mediated gene expression and toxicity[J]. Chemosphere, 2008, 73: 209-215. doi: 10.1016/j.chemosphere.2008.05.025
    [50]
    MERSON R R, KARCHNER S I, HAHN M E. Interaction of fish aryl hydrocarbon receptor paralogs (AHR1 and AHR2) with the retinoblastoma protein[J]. Aquat Toxicol, 2009, 94(1): 47-55. doi: 10.1016/j.aquatox.2009.05.015
    [51]
    ROY N K, CANDELMO A, DELLATORRE M, et al. Characterization of AHR2 and CYP1A expression in Atlantic sturgeon and shortnose sturgeon treated with coplanar PCBs and TCDD[J]. Aquat Toxicol, 2018, 197: 19-31. doi: 10.1016/j.aquatox.2018.01.017
  • Related Articles

    [1]CAI Runji, PENG Xiaohong, YE Shuangfu, ZHANG Tianchen, GAO Yuefang, LYU Junlin. Automatic counting of shrimp larvae based on probability density map model generated from front end and back end[J]. South China Fisheries Science, 2025, 21(1): 173-184. DOI: 10.12131/20240212
    [2]XU Bo, YUAN Hongchun. Research on fish feeding intensity classification model based on axial feature calibration and temporal segment network[J]. South China Fisheries Science, 2024, 20(6): 145-154. DOI: 10.12131/20240200
    [3]Lihao TONG, Xiangyu WU, Liangfu HUANG, Jun ZENG, Yaohua SHI, Xianming TANG. Correlation analysis of light intensity and growth, photosynthetic pigment, color value of Betaphycus gelatinae[J]. South China Fisheries Science, 2021, 17(5): 79-85. DOI: 10.12131/20200256
    [4]CHEN Jian, GUO Dan, ZHAI Ziqing, YU Dahui, BAI Lirong. Correlation analysis of morphological traits and body mass traits of Coelomactra antiquata[J]. South China Fisheries Science, 2021, 17(1): 45-51. DOI: 10.12131/20200180
    [5]ZHENG Xing, LIN Siqi, YANG Shouguo, ZHANG Xingzhi, VASQUEZ Herbert Ely, GU Zhifeng, WANG Aimin. Change and correlation analysis of pigment contents and color value during growth of Chlorella vulgaris[J]. South China Fisheries Science, 2021, 17(1): 32-38. DOI: 10.12131/20200076
    [6]LU Han, LUO Yongkang, SHI Ce, FENG Ligeng. Correlation between impedance and freshness indicators of silver carp (Hypophthalmichthys molitrix) during 0 ℃ storage[J]. South China Fisheries Science, 2012, 8(5): 80-85. DOI: 10.3969/j.issn.2095-0780.2012.05.012
    [7]CHEN Jinling, LAI Qiuming, SU Shuye, KE Yangyong. Study on variation characteristics and correlation analysis of major ecological factors in intensive shrimp ponds[J]. South China Fisheries Science, 2012, 8(4): 49-56. DOI: 10.3969/j.issn.2095-0780.2012.04.008
    [8]LIU Chaoyang, WANG Yingeng, SUN Xiaoqing. Correlation between bacteria associated with trash fish and diseases of cultured turbot(Scophthalmus maximus)[J]. South China Fisheries Science, 2009, 5(5): 44-51. DOI: 10.3969/j.issn.1673-2227.2009.05.008
    [9]LIU Chaoyang, WANG Yingeng, SUN Xiaoqing. Correlation between bacteria in feed pellets and diseases of cultured turbot Scophthalmus maximus[J]. South China Fisheries Science, 2009, 5(4): 13-21. DOI: 10.3969/j.issn.1673-2227.2009.04.003
    [10]WU Fengxia, LI Chunhou, DAI Ming. Application of artificial neural networks ocean sciences[J]. South China Fisheries Science, 2009, 5(1): 75-80. DOI: 10.3969/j.issn.1673-2227.2009.01.013
  • Cited by

    Periodical cited type(9)

    1. 史银魁,俞立雄,周雪,高雷,朱峰跃,杨锦毅,陈大庆,王珂,段辛斌. 禁渔初期长江宜昌-城陵矶江段鱼类资源时空分布特征. 水生生物学报. 2024(04): 546-558 .
    2. 张丽媛,熊清海,田敏,刘淑伟,王慧,曲品,蒋荣明,杨剑虹. 阳宗海鱼类群落结构及变化. 云南农业大学学报(自然科学). 2024(02): 60-69 .
    3. 史艳萍,屈婵娟,许旺,周春花,梁璐,王宇翔,任文伟,戴年华,徐晓娟,黎栩霞,计勇,吴小平,金斌松. 基于环境DNA宏条形码的青岚湖自然保护区鱼类组成及分布特征. 中国环境监测. 2024(03): 235-246 .
    4. 曹寿清,杨思雅,胡江春,杨慕静. 泸沽湖小口裂腹鱼人工驯养繁育试验. 水产养殖. 2024(07): 44-47 .
    5. 张航,梁智策,匡晨亿,周婷,廖传松,苑晶,郭传波,刘家寿. 基于水声学和渔获物调查的洱海鱼类资源时空分布特征. 水生生物学报. 2024(12): 2029-2041 .
    6. 贾春艳,王珂,李慧峰,高雷,杨浩,刘绍平,陈大庆,段辛斌. 禁渔初期东洞庭湖鱼类资源的空间分布与密度变化. 南方水产科学. 2022(03): 48-56 . 本站查看
    7. 王书献,张胜茂,戴阳,王永进,隋江华,朱文斌. 利用声呐数据提取磷虾捕捞深度方法研究. 南方水产科学. 2021(04): 91-97 . 本站查看
    8. 梁祥,薛绍伟,武智,王静,张建斌,赖宝衡,胡智光. 云龙水库鱼类资源声学评估. 西南农业学报. 2021(09): 2057-2062 .
    9. 徐川,胡正春. 核电厂冷源安全渔业资源声学调查. 电力安全技术. 2020(10): 55-59 .

    Other cited types(2)

Catalog

    Article views (588) PDF downloads (35) Cited by(11)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return