Volume 18 Issue 4
Aug.  2022
Turn off MathJax
Article Contents
Abstract
References
Related Articles
ZHANG Zhe, GONG Xiuyu, LAN Lili, WANG Xuefeng, MA Shengwei, CHEN Haigang, ZHANG Linbao. Cloning and expression profiling of Cyp1a gene in Lutjanus argentimaculatus under 4-bromodiphenyl ether (BDE-3) and decabromodiphenyl ether (BDE-209) exposure[J]. South China Fisheries Science, 2022, 18(4): 54-64. DOI: 10.12131/20210271
Citation: ZHANG Zhe, GONG Xiuyu, LAN Lili, WANG Xuefeng, MA Shengwei, CHEN Haigang, ZHANG Linbao. Cloning and expression profiling of Cyp1a gene in Lutjanus argentimaculatus under 4-bromodiphenyl ether (BDE-3) and decabromodiphenyl ether (BDE-209) exposure[J]. South China Fisheries Science, 2022, 18(4): 54-64. DOI: 10.12131/20210271
shu

Cloning and expression profiling of Cyp1a gene in Lutjanus argentimaculatus under 4-bromodiphenyl ether (BDE-3) and decabromodiphenyl ether (BDE-209) exposure

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment/Scientific Observing and Experimental Station of South China Sea Fishery Resource and Environment, Ministry of Agriculture and Rural Affairs/Scientific Observation and Research Field Station of Pearl River Estuary Ecosystem, Guangzhou 510300, China

  • 2.

    Guangzhou Customs Technology Center, Guangzhou 510623, China

  • 3.

    Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China

  • 4.

    College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China

More Information
  • Received Date: September 21, 2021
  • Revised Date: November 03, 2021
  • Accepted Date: November 30, 2021
  • Available Online: December 13, 2021
    Graphical Abstract
    • Abstract
  • Cytochrome P450 enzymes (CYPs) are encoded by P450 genes, in which cytochrome P450 1A (Cyp1a) genes mainly involve in biotransformation and metabolism of numerous xenobiotics. In this study, we cloned the Cyp1a gene from Lutjanus argentimaculatus, and investigated its tissue expression pattern. In addition, we evaluated different concentrations (10, 50 and 250 μg·L−1) of BDE-3 and BDE-209 on Cyp1a gene profile and 7-ethoxyresorufin-O-deethylase (EROD) activity in liver of L. argentimaculatus. The results show that the full length of Cyp1a cDNA was 2 540 bp with 1 566 open reading frame encoding 521 amino acids. The sequence homology of L. argentimaculatus CYP1A was the highest (92.69%) with that of Lateolabrax maculatus. Phylogenetic analysis results indicate that CYP1A was closely aligned with Sander lucioperca protein. Cyp1a transcripts were most abundant in liver, followed by brain and gill, but lowest in muscle. 10 μg·L−1 of BDE-3 and BDE-209 showed no effects on both Cyp1a expression and EROD activity, while high concentrations (50 and 250 μg·L−1) of BDE-3 down-regulated both of them significantly in a concentration-dependent manner on 7th-15th day. In contrast, exposure to 50 and 250 μg·L−1 of BDE-209 resulted in increasing of hepatic Cyp1a level and EROD activity. Moreover, Cyp1a genes levels and EROD activities showed a good correlation. High concentrations of BDE-3 and BDE-209 can affect Cyp1a gene expression in liver of L. argentimaculatus in different manners.
    • FullText(HTML)
    • References (51)
  • [1]
    LU K, SONG Y, ZENG R. The role of cytochrome P450-mediated detoxification in the insect adaptation to xenobiotics[J]. Curr Opin Insect Sci, 2021, 43: 103-107. doi: 10.1016/j.cois.2020.11.004
    [2]
    LAU I C K, FEYEREISEN R, NELSON D R, et al. Analysis and preliminary characterisation of the cytochrome P450 monooxygenases from Frankia sp. EuI1c (Frankia inefficax sp. )[J]. Arch Biochem Biophys, 2019, 669: 11-21. doi: 10.1016/j.abb.2019.05.007
    [3]
    MILLER J C, HOLLATZ A J, SCHULER M A. P450 variations bifurcate the early terpene indole alkaloid pathway in Catharanthus roseus and Camptotheca acuminate[J]. Phytochemistry, 2021, 183: 112626. doi: 10.1016/j.phytochem.2020.112626
    [4]
    YANG T, LI T, FENG X, et al. Multiple cytochrome P450 genes: conferring high levels of permethrin resistance in mosquitoes, Culex quinquefasciatus[J]. Sci Rep, 2021, 11(1): 9041. doi: 10.1038/s41598-021-88121-x
    [5]
    NELSON D R. Cytochrome P450 diversity in the tree of life[J]. Biochim Biophys Acta Proteins Proteom, 2018, 1866(1): 141-154. doi: 10.1016/j.bbapap.2017.05.003
    [6]
    张文领, 牟希东, 胡隐昌, 等. 福寿螺细胞色素P450 基因CYP3192A1 的克隆与表达分析[J]. 南方水产科学, 2017, 13(1): 66-75. doi: 10.3969/j.issn.2095-0780.2017.01.009
    [7]
    HAN J, KIM D, KIM H, et al. Genome-wide identification of 52 cytochrome P450 (CYP) genes in the copepod Tigriopus japonicus and their B[α]P-induced expression patterns[J]. Comp Biochem Physiol D, 2017, 23: 49-57.
    [8]
    高锴, 闫佩, 檀翠玲, 等. 虹鳟鱼鳃及肝脏多种CYP1 基因表达模式作为生物标志物监测海河水污染状况[J]. 环境科学, 2015, 36(10): 3878-3883.
    [9]
    PENG F, HARDY E M, BÉRANGER R, et al. Human exposure to PCBs, PBDEs and bisphenols revealed by hair analysis: a comparison between two adult female populations in China and France[J]. Environ Pollut, 2020, 267: 115425. doi: 10.1016/j.envpol.2020.115425
    [10]
    DA C, WANG R, XIA L, et al. Sediment records of polybrominated diphenyl ethers (PBDEs) in Yangtze River Delta of Yangtze River in China[J]. Mar Pollut Bull, 2021, 160: 111714.
    [11]
    LIU B, SONG N, JIANG T, et al. Polybrominated diphenyl ethers in surface sediments from fishing ports along the coast of Bohai Sea, China[J]. Mar Pollut Bull, 2021, 164: 112037. doi: 10.1016/j.marpolbul.2021.112037
    [12]
    YUAN J, SUN X, CHE S, et al. AhR-mediated CYP1A1 and ROS overexpression are involved in hepatotoxicity of decabromodiphenyl ether (BDE-209)[J]. Toxicol Lett, 2021, 352: 26-33. doi: 10.1016/j.toxlet.2021.09.008
    [13]
    LI Y, MA F, LI Z, et al. Exposure to 4-bromodiphenyl ether during pregnancy blocks testis development in male rat fetuses[J]. Toxicol Lett, 2021, 342: 38-49. doi: 10.1016/j.toxlet.2021.02.004
    [14]
    YAO Y, WANG B, HE Y, et al. Fate of 4-bromodiphenyl ether (BDE3) in soil and the effects of co-existed copper[J]. Environ Pollut, 2020, 261: 114214. doi: 10.1016/j.envpol.2020.114214
    [15]
    李嘉伟, 尹晓宇, 周旖旎, 等. 五溴联苯醚(BDE-99)和羟基五溴联苯醚(5-OH-BDE-99)经由THRβ影响斑马鱼胚胎眼部色素的沉着[J]. 生态毒理学报, 2020, 15(5): 181-188.
    [16]
    THORNTON L M, PATH E M, NYSTROM G S, et al. Embryo-larval BDE-47 exposure causes decreased pathogen resistance in adult male fathead minnows (Pimephales promelas)[J]. Fish Shellfish Immunol, 2018, 80: 80-87. doi: 10.1016/j.fsi.2018.05.059
    [17]
    王余江, 樊琳, 陈创奇, 等. 视黄酸和多溴联苯醚联合暴露对斑马鱼运动行为的影响[J]. 生态毒理学报, 2019, 14(2): 260-267. doi: 10.7524/AJE.1673-5897.20180206002
    [18]
    YANG J, ZHAO H, CHAN K M. Toxic effects of polybrominated diphenyl ethers (BDE 47 and 99) and localization of BDE-99-induced cyp1a mRNA in zebrafish larvae[J]. Toxicol Rep, 2017, 4: 614-624. doi: 10.1016/j.toxrep.2017.11.003
    [19]
    SØFTELAND L, PETERSEN K, STAVRUM A, et al. Hepatic in vitro toxicity assessment of PBDE congeners BDE47, BDE153 and BDE154 in Atlantic salmon (Salmo salar L. )[J]. Aquat Toxicol, 2011, 105(3/4): 246-263.
    [20]
    BOON J P, ZANDEN J J, LEWIS W E, et al. The expression of CYP1A, vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses[J]. Mar Environ Res, 2002, 54(3-5): 719-724. doi: 10.1016/S0141-1136(02)00127-7
    [21]
    MUYOT F B, MAGISTRADO M L, MUYOT M C, et al. Growth performance of the mangrove red snapper (Lutjanus argentimaculatus) in freshwater pond comparing two stocking densities and three feed types[J]. Philippine J Fish, 2021, 28(1): 1-7.
    [22]
    CHEN H, ZHANG Z, ZHANG L, et al. Effects of di-n-butyl phthalate on gills- and liver-specific EROD activities and CYP1A levels in juvenile red snapper (Lutjanus argentimaculatus)[J]. Comp Biochem Physiol C, 2020, 232: 108757.
    [23]
    KUMAR S, STECHER G, TAMURA K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Mol Biol Evol, 2016, 33(7): 1870-1874. doi: 10.1093/molbev/msw054
    [24]
    余铭恩, 郑榕辉, 张玉生. 3种海洋鱼类肝微粒体EROD活性的测定[J]. 生态学报, 2014, 34(19): 5416-5424.
    [25]
    LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262
    [26]
    CHAUBE R, RAWAT A, INBARAJ R M, et al. Cloning and characterization of estrogen hydroxylase (cyp1a1 and cyp1b1) genes in the stinging catfish Heteropneustes fossilis and induction of mRNA expression during final oocyte maturation[J]. Comp Biochem Physiol A, 2021, 253: 110863. doi: 10.1016/j.cbpa.2020.110863
    [27]
    ARUKWE A. Complementary DNA cloning, sequence analysis and differential organ expression of β-naphthoflavone-inducible cytochrome P4501A in Atlantic salmon (Salmo salar)[J]. Comp Biochem Physiol C, 2002, 133(4): 613-624.
    [28]
    WOO S J, CHUNG J K. Cytochrome P450 1 enzymes in black rockfish, Sebastes schlegelii: molecular characterization and expression patterns after exposure to benzo[a]pyrene[J]. Aquat Toxicol, 2020, 226: 105566. doi: 10.1016/j.aquatox.2020.105566
    [29]
    COCCI P, MOSCONI G, PALERMO F A. Effects of 4-nonylphenol on hepatic gene expression of peroxisome proliferator-activated receptors and cytochrome P450 isoforms (CYP1A1 and CYP3A4) in juvenile sole (Solea solea)[J]. Chemosphere, 2013, 93(6): 1176-1181. doi: 10.1016/j.chemosphere.2013.06.058
    [30]
    梁秋芳, 董小燕, 冯平. CYP2D 亚家族基因及其进化机制研究进展[J]. 广西师范大学学报 (自然科学版), 2021, 39(5): 58-63.
    [31]
    BURKIAN V, ZAMARATSKAIA G, SAKALLI S, et al. Tissue-specific expression and activity of cytochrome P450 1A and 3A in rainbow trout (Oncorhynchus mykiss)[J]. Toxicol Lett, 2021, 341: 1-10. doi: 10.1016/j.toxlet.2021.01.011
    [32]
    RUSNI S, SASSA M, TAKEHANA Y, et al. Correlation between cytochrome P450 1A (cyp1a) mRNA expression and ambient phenanthrene and pyrene concentration in Javanese medaka Oryzias javanicus[J]. Fish Sci, 2020, 86: 605-613. doi: 10.1007/s12562-020-01428-y
    [33]
    KIM R, KIM B, HWANG D, et al. Evaluation of biomarker potential of cytochrome P450 1A (CYP1A) gene in the marine medaka, Oryzias melastigma exposed to water-accommodated fractions (WAFs) of Iranian crude oil[J]. Comp Biochem Physiol C, 2013, 157(2): 172-182.
    [34]
    PETRULIS J R, CHEN G, BENN S, et al. Application of the ethoxyresorufin-O-deethylase (EROD) assay to mixtures of halogenated aromatic compounds[J]. Environ Toxicol, 2001, 16(2): 177-184. doi: 10.1002/tox.1022
    [35]
    ROY M A, SANT K E, VENEZIA O L, et al. The emerging contaminant 3, 3'-dichlorobiphenyl (PCB-11) impedes Ahr activation and Cyp1a activity to modify embryotoxicity of Ahr ligands in the zebrafish embryo model (Danio rerio)[J]. Environ Pollut, 2019, 254: 113027. doi: 10.1016/j.envpol.2019.113027
    [36]
    SMITH E M, IFTIKAR F I, HIGGINS S, et al. In vitro inhibition of cytochrome P450-mediated reactions by gemfibrozil, erythromycin, ciprofloxacin and fluoxetine in fish liver microsomes[J]. Aquat Toxicol, 2012, 109: 259-266. doi: 10.1016/j.aquatox.2011.08.022
    [37]
    DAR S A, GORA A H, BHAT I A, et al. Studies of anthelminthic benzimidazole derivatives on cytochrome P450 1A (CYP1A) dependent detoxification mechanism in Labeo rohita[J]. Aquaculture, 2017, 481: 79-84. doi: 10.1016/j.aquaculture.2017.08.015
    [38]
    迟潇, 陈碧娟, 孙雪梅, 等. 基于IBR模型研究BDE-47和BDE-153对半滑舌鳎的毒性效应[J]. 生态毒理学报, 2020, 15(4): 192-202.
    [39]
    VEN L T M V, KUIL T, LEONARDS P E G, et al. A 28-day oral dose toxicity study in Wistar rats enhanced to detect endocrine effects of decabromodiphenyl ether (decaBDE)[J]. Toxicol Lett, 2008, 179(1): 6-14. doi: 10.1016/j.toxlet.2008.03.003
    [40]
    WANG B, WANG H, XIAO D, et al. In vitro effects of brominated flame retardants, selected metals and their mixtures on ethoxyresorufin-O-deethylase activity in Mossambica tilapia liver[J]. Ecotoxicol Environ Saf, 2018, 161: 350-355. doi: 10.1016/j.ecoenv.2018.05.084
    [41]
    黄志斐, 马胜伟, 张喆, 等. BDE3胁迫对翡翠贻贝 (Perna viridis) SOD、MDA和GSH的影响[J]. 南方水产科学, 2012, 8(5): 25-30. doi: 10.3969/j.issn.2095-0780.2012.05.004
    [42]
    XIE Z, LU G, QI P. Effects of BDE-209 and its mixtures with BDE-47 and BDE-99 on multiple biomarkers in Carassius auratus[J]. Environ Toxicol Pharmacol, 2014, 38(2): 554-561. doi: 10.1016/j.etap.2014.08.008
    [43]
    YANG J, ZHU J, CHAN K M. BDE-99, but not BDE-47, is a transient aryl hydrocarbon receptor agonist in zebrafish liver cells[J]. Toxicol Appl Pharmacol, 2016, 305: 203-215. doi: 10.1016/j.taap.2016.06.023
    [44]
    SHARIFIAN S, HOMAEI A, KAMRANI E, et al. New insights on the marine cytochrome P450 enzymes and their biotechnological importance[J]. Int Biol Macromol, 2020, 142: 811-821. doi: 10.1016/j.ijbiomac.2019.10.022
    [45]
    SÁNCHEZ-OCAMPO E M, AZUELA G E, SALAS M S, et al. Alterations in viability and CYP1A1 expression in SH SY5Y cell line by pollutants present in Madín Dam, Mexico[J]. Sci Total Environ, 2020, 719: 137500. doi: 10.1016/j.scitotenv.2020.137500
    [46]
    LI Z, ZHONG L, MU W, et al. Effects of chronic exposure to tributyltin on tissue specific cytochrome P450 1 regulation in juvenile common carp[J]. Xenobiotica, 2016, 46(6): 511-515. doi: 10.3109/00498254.2015.1092618
    [47]
    CAPPELLETTI N, SPERANZA E, TATONE L, et al. Bioaccumulation of dioxin-like PCBs and PBDEs by detritus-feeding fish in the Rio de la Plata estuary, Argentina[J]. Environ Sci Pollut Res Int, 2015, 22(9): 7093-7100. doi: 10.1007/s11356-014-3935-z
    [48]
    KUIPER R V, BERGMAN Å, VOS J G, et al. Some polybrominated diphenyl ether (PBDE) flame retardants with wide environmental distribution inhibit TCDD-induced EROD activity in primary cultured carp (Cyprinus carpio) hepatocytes[J]. Aquat Toxicol, 2004, 68(2): 129-139. doi: 10.1016/j.aquatox.2004.03.005
    [49]
    WHAL M, LAHNI B, GUENTHER R, et al. A technical mixture of 2, 2', 4, 4'-tetrabromo diphenyl ether (BDE47) and brominated furans triggers aryl hydrocarbon receptor (AhR) mediated gene expression and toxicity[J]. Chemosphere, 2008, 73: 209-215. doi: 10.1016/j.chemosphere.2008.05.025
    [50]
    MERSON R R, KARCHNER S I, HAHN M E. Interaction of fish aryl hydrocarbon receptor paralogs (AHR1 and AHR2) with the retinoblastoma protein[J]. Aquat Toxicol, 2009, 94(1): 47-55. doi: 10.1016/j.aquatox.2009.05.015
    [51]
    ROY N K, CANDELMO A, DELLATORRE M, et al. Characterization of AHR2 and CYP1A expression in Atlantic sturgeon and shortnose sturgeon treated with coplanar PCBs and TCDD[J]. Aquat Toxicol, 2018, 197: 19-31. doi: 10.1016/j.aquatox.2018.01.017
    • Related Articles

  • Related Articles

    [1]LI Jiangtao, ZHANG Yanqiu, ZHANG Hong, LIU Chun, QIU Xiaolong, CHEN Ming, FANG Junchao, QIU Xiaotong, LIN Li, LYU Xiaojing. Effects of density stress on swimming behavior and muscle energy metabolism of Micropterus salmoides[J]. South China Fisheries Science, 2024, 20(2): 102-110. DOI: 10.12131/20230176
    [2]BAO Junjie, WANG Yongjie, CHEN Honglian, SUN Wen, ZHANG Jing, ZHOU Beibei. Untargeted metabolomics analysis of metabolic differences of crayfish (Procambarus clarkii)  meat with different diets[J]. South China Fisheries Science, 2023, 19(5): 104-112. DOI: 10.12131/20230055
    [3]CHEN Zhizhao, ZHU Tao, LEI Caixia, JIANG Peng, DU Jinxing, ZHU Junjie, SONG Hongmei, LI Shengjie. Effects on growth and hepatic glucose metabolism of grass carp fed with high dietary carbohydrates[J]. South China Fisheries Science, 2023, 19(5): 75-85. DOI: 10.12131/20230020
    [4]LIU Jiaxing, GUO Huayang, ZHU Kecheng, LIU Baosuo, ZHANG Nan, XIAN Lin, ZHANG Dianchang. Effects of cysteine addition to low-fishmeal diets on metabolism of lipid and protein in juvenile Trachinotus ovatus[J]. South China Fisheries Science, 2023, 19(4): 116-125. DOI: 10.12131/20230030
    [5]CHEN Li, XU Jiaxin, LI Liujia, ZHAO Chengfa, LONG Xiaowen. Effects of dietary fishmeal replacement by Periplaneta americana meal on biochemical indexes, disease resistance and metabolomics of juvenile Oncorhynchus mykiss[J]. South China Fisheries Science, 2023, 19(4): 86-97. DOI: 10.12131/20220208
    [6]LIU Guangxin, DONG Yanjun, ZHAO Lijuan, DENG Yiqin, CHENG Changhong, MA Hongling, JIANG Jianjun, FENG Juan, GUO Zhixun, LIN Li. Sequencing of whole genome of Bacillus velezensis LG37 and screening of inorganic nitrogen metabolism candidate genes[J]. South China Fisheries Science, 2022, 18(3): 57-67. DOI: 10.12131/20210149
    [7]ZHANG Guilin, ZHANG Yating, JIANG Hong, LIU Zhen, MAO Xiangzhao. Metabolic engineering synthesis of neoxanthin, a key precursor of fucoxanthin[J]. South China Fisheries Science, 2022, 18(2): 57-65. DOI: 10.12131/20210316
    [8]SUN Caiyun, DONG Hongbiao, WANG Wenhao, LI Yong, GU Qunhong, DUAN Yafei, ZHANG Jiasong, XU Xiaodong. Effects of glycerol monolaurate on lipid metabolism of Lateolabrax maculatus[J]. South China Fisheries Science, 2021, 17(1): 67-75. DOI: 10.12131/20200130
    [9]CHEN Zicong, CHEN Pimao, YUAN Huarong, FENG Xue, TONG Fei, ZHANG Haoming. Study on respiratory metabolism changes of juvenile Penaeus monodon following strenuous activity[J]. South China Fisheries Science, 2020, 16(4): 75-83. DOI: 10.12131/20200017
    [10]LIU Yong, SHI Kuntao, ZHANG Shaohua, YUAN Yongdang. Advancement of respiratory metabolism study in bivalve mollusus[J]. South China Fisheries Science, 2007, 3(4): 65-69.

  • Cited by

    Periodical cited type(20)

    1. 田思泉，柳晓雪，花传祥，王寅，杜涣洋. 南海渔业资源状况及其管理挑战. 上海海洋大学学报. 2024(03): 786-798 .
    2. 刘子凯，许友伟，蔡研聪，孙铭帅，张魁，陈作志. 基于长度数据的南海北部深水金线鱼资源评估. 南方水产科学. 2024(04): 24-33 . 本站查看
    3. 张曼，王雪辉，王淼娣，杜飞雁，孙典荣，王亮根，王跃中，许柳雄，邱永松. 基于长度贝叶斯生物量估算法的北部湾带鱼资源评估. 海洋学报. 2022(01): 11-21 .
    4. 李亚男，杨炳忠，张鹏，李杰，王腾，晏磊. 南海北部拖网对蓝圆鲹的选择性研究. 南方水产科学. 2022(03): 170-176 . 本站查看
    5. 史登福，张魁，蔡研聪，许友伟，孙铭帅，徐姗楠，朱江峰，陈作志. 数据有限条件下珠江口棘头梅童鱼资源状况评估. 海洋渔业. 2022(04): 435-445 .
    6. 崔明远，田思泉，麻秋云，范青松. 基于单位补充量模型的浙江南部海域蓝圆鲹资源评价. 水产科学. 2022(05): 727-737 .
    7. 王薇，陈国宝，牛麓连. 不同捕捞方式下南海北部海域鲹类渔场的时空分布. 广东海洋大学学报. 2022(06): 74-80 .
    8. 粟丽，陈作志，张魁，许友伟，邱永松. 基于底拖网调查数据的渔业资源质量状况评价体系构建——以北部湾为例. 广东海洋大学学报. 2021(01): 10-16 .
    9. 邓裕坚，易木荣，李波，刘思杓，邱康文，沈春燕，何雄波，颜云榕. 北部湾春季多齿蛇鲻生物学特征及其年际变化. 渔业科学进展. 2021(02): 36-44 .
    10. 何雄波，李波，王锦溪，易木荣，康斌，颜云榕. 不同时期北部湾日本带鱼营养生态位差异. 应用生态学报. 2021(02): 683-690 .
    11. 王开立，陈作志，许友伟，孙铭帅，王欢欢，蔡研聪，张魁，徐姗楠. 南海北部近海蓝圆鲹渔业生物学特征研究. 海洋渔业. 2021(01): 12-21 .
    12. 吴新燕，梁宏伟，罗相忠，沙航，邹桂伟. 不同月龄长丰鲢形态性状对体质量的影响. 南方水产科学. 2021(03): 62-69 . 本站查看
    13. 史登福，许友伟，孙铭帅，黄梓荣，陈作志，张魁. 广东海洋渔业资源可捕量评估. 海洋渔业. 2021(05): 521-531 .
    14. 熊朋莉，陈作志，侯刚，张帅，邱永松，范江涛，徐姗楠. 珠江河口棘头梅童鱼生物学特征的年代际变化. 南方水产科学. 2021(06): 31-38 . 本站查看
    15. 朱书礼，李跃飞，武智，李捷，夏雨果，杨计平，李新辉. 基于体长频率数据的西江封开段赤眼鳟可捕规格与资源保护研究. 南方水产科学. 2020(04): 1-7 . 本站查看
    16. 洪小帆，张俊，江艳娥，蔡研聪，杨玉滔，王欢欢，李纲，陈作志. 南海西沙群岛琛航岛犬牙锥齿鲷生物学特征. 生态学杂志. 2020(10): 3320-3331 .
    17. 史登福，张魁，蔡研聪，耿平，许友伟，孙铭帅，陈作志. 南海北部带鱼群体结构及生长、死亡和性成熟参数估计. 南方水产科学. 2020(05): 51-59 . 本站查看
    18. 崔明远，陈伟峰，戴黎斌，麻秋云. 浙江南部海域蓝圆鲹生长异质性及死亡特征. 中国水产科学. 2020(12): 1427-1437 .
    19. 李忠炉，张文旋，何雄波，颜云榕. 南海北部湾秋季蓝圆鲹与竹筴鱼的摄食生态及食物竞争. 广东海洋大学学报. 2019(03): 79-86 .
    20. 王言丰，余景，陈丕茂，于杰，刘祝楠. 北部湾灯光罩网渔场时空分布与海洋环境关系分析. 热带海洋学报. 2019(05): 68-76 .

    Other cited types(13)

Catalog

    Get Citation
    PDF
    XML
    Article views (588) PDF downloads (35) Cited by(33)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return