Volume 18 Issue 3
Jun.  2022
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CHEN Xiaolei, LI Min, CHEN Zuozhi, ZHANG Jun, ZHANG Shuai, QI Zhanhui, XU Shannan. Preliminary metabacording dietary analysis of Diaphus splendidus in South China Sea[J]. South China Fisheries Science, 2022, 18(3): 22-29. DOI: 10.12131/20210199
Citation: CHEN Xiaolei, LI Min, CHEN Zuozhi, ZHANG Jun, ZHANG Shuai, QI Zhanhui, XU Shannan. Preliminary metabacording dietary analysis of Diaphus splendidus in South China Sea[J]. South China Fisheries Science, 2022, 18(3): 22-29. DOI: 10.12131/20210199
shu

Preliminary metabacording dietary analysis of Diaphus splendidus in South China Sea

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory for Sustainable Utilization of Open-Sea Fishery, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

  • 3.

    Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China

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  • Received Date: July 18, 2021
  • Revised Date: September 21, 2021
  • Accepted Date: October 07, 2021
  • Available Online: October 17, 2021
    Graphical Abstract
    • Abstract
  • Dietary analysis of fish is an important part of fish biology and ecology as well as the basis of fishery resources development. In this study, the gastric DNA of Diaphus splendidus was extracted, and the mitochondrial cytochrome c oxidase subunit I (COI) was amplified as a molecular marker. The food composition was identified by high-throughput sequencing. The results show that a total of 34 species belonged to 5 phyla, 7 classes, 11 orders, 18 families and 29 genera. The diet of D. splendidus mainly included Ostracoda, Copepoda, Amphipoda, Fish, Jellyfish, Euphausiacea, Gastropod and Polychaeta. Ostracoda, Copepoda and Amphipoda were the dominant groups in its food composition, which is basically consistent with the previous research based on traditional morphological identification, except for jellyfish that was identified only by this study. In conclusion, DNA metabarcoding method is suitable for the food analysis of D. splendidus in the South China Sea, and has higher identification potential than the food analysis based on morphology.
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    • References (39)
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