Volume 18 Issue 3
Jun.  2022
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SI Mengru, LI Yundong, YANG Qibin, JIANG Song, YANG Lishi, HUANG Jianhua, JIANG Shigui, ZHOU Falin. Characterization and expression analysis of PP2C from Penaeus monodon under acute low salt and ammonia nitrogen stress[J]. South China Fisheries Science, 2022, 18(3): 76-85. DOI: 10.12131/20210193
Citation: SI Mengru, LI Yundong, YANG Qibin, JIANG Song, YANG Lishi, HUANG Jianhua, JIANG Shigui, ZHOU Falin. Characterization and expression analysis of PP2C from Penaeus monodon under acute low salt and ammonia nitrogen stress[J]. South China Fisheries Science, 2022, 18(3): 76-85. DOI: 10.12131/20210193
shu

Characterization and expression analysis of PP2C from Penaeus monodon under acute low salt and ammonia nitrogen stress

  • 1.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 3.

    Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China

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  • Received Date: July 04, 2021
  • Revised Date: August 31, 2021
  • Accepted Date: October 08, 2021
  • Available Online: October 25, 2021
    Graphical Abstract
    • Abstract
  • Protein phosphatase 2C family protein is a class of protein phosphatases that plays an important role in stress resistance. However, there are few studies in crustaceans. In this study, we cloned and obtained the full-length cDNA of protein phosphatase 2C (PmPP2C) with c-type domain in Penaeus monodon by rapid amplification of cDNA ends (RACE). The full length of ORF was 2 079 bp, encoding 692 amino acids. The real-time fluorescence quantitative results show that PmPP2C was expressed in all the tested tissues. The expression was the highest in hepatopancreas and gill tissues, followed by thoracic nerve, testis and muscle. The expression of PmPP2C in hepatopancreas and gill was first down-regulated and then up-regulated under 96 h acute low salt stress. The expression of PmPP2C in hepatopancreas and gill showed a downward-upward-downward trend under 96 h acute ammonia nitrogen stress. The results indicate that PmPP2C can be involved in the response of P. monodon to acute low salt and ammonia nitrogen stress, suggesting that it may play an important role in the immune defense against environmental stress.
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