Volume 18 Issue 3
Jun.  2022
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GAO Shufang, ZHANG Jinpeng, SHI Yonghai, YUAN Xincheng, LIU Qigen. Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry[J]. South China Fisheries Science, 2022, 18(3): 68-75. DOI: 10.12131/20210185
Citation: GAO Shufang, ZHANG Jinpeng, SHI Yonghai, YUAN Xincheng, LIU Qigen. Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry[J]. South China Fisheries Science, 2022, 18(3): 68-75. DOI: 10.12131/20210185
shu

Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry

  • 1.

    Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education/Centre for Research on Environmental Ecology and Fish Nutrition, Ministry of Agriculture and Rural Affairs/Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China

  • 2.

    Shanghai Fisheries Research Institute/Shanghai Fisheries Technology Extension Station, Shanghai 200433, China

More Information
  • Received Date: June 23, 2021
  • Revised Date: October 08, 2021
  • Accepted Date: November 03, 2021
  • Available Online: November 09, 2021
    Graphical Abstract
    • Abstract
  • In order to clarify the effects of seawater and freshwater on the ovary development of Coilia nasus, we analyzed their differences by using non-targeted metabolomics, and compared with database of KEGG directly to find out the corresponding metabolic pathways, then analyzed its causes. The results show that a total of 47 metabolites had significant difference between the two groups (P<0.05, FC>1, VIP>1). Compared with the seawater group, the most significant differences in the expression were carbocyclic thromboxane A2, Galactosylceramide, and their differences were 10.40 and 2.78 times, respectively. The cortisol in the ovarian tissue of the freshwater group increased by 1.61 times. According to the analysis on KEGG metabolic pathways of 47 different metabolites, the biosynthesis of aminoacyl-tRNA and pyridine metabolic pathways changed in the seawater and freshwater environments significantly (P<0.05). The biosynthesis pathway of cortisol, aminoyl-tRNA, pyrimidine metabolism pathway and sphingo-lipid metabolism pathway may be related to the ovarian development during the reproductive migration of C. nasus.
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    • References (39)
  • [1]
    SU M, DUAN Z, SHI H, et al. The effects of salinity on reproductive development and egg and larvae survival in the spotted scat Scatophagus argus under controlled conditions[J]. Aquac Res, 2019, 50(7): 1782-1794. doi: 10.1111/are.14056
    [2]
    MOHANTY B, GUPTA K, BABU G, et al. Exposure to salinity stress cause ovarian disruption in a stenohaline freshwater teleost, Heteropneustes fossilis (Bloch, 1794)[J]. Aquac Res, 2020, 51(5): 1964-1972. doi: 10.1111/are.14548
    [3]
    PHAM H Q, KJØRSVIK E, NGUYEN A T, et al. Reproductive cycle in female Waigieu seaperch (Psammoperca waigiensis) reared under different salinity levels and the effects of dopamine antagonist on steroid hormone levels[J]. J Exp Mar Biol Ecol, 2010, 383(2): 137-145. doi: 10.1016/j.jembe.2009.12.010
    [4]
    PHAM H Q, NGUYEN A T, KJØRSVIK E, et al. Seasonal reproductive cycle of Waigieu seaperch (Psammoperca waigiensis)[J]. Aquac Res, 2012, 43(6): 815-830. doi: 10.1111/j.1365-2109.2011.02894.x
    [5]
    牛景彦, 刘占才. 影响鱼类性腺发育的生态因素研究[J]. 农业与技术, 2016, 36(16): 109.
    [6]
    杜学芳. 盐度对凡纳滨对虾繁殖及家系生长、存活的影响[D]. 上海: 上海海洋大学, 2013: 9-17.
    [7]
    吴旭干, 赵亚婷, 何杰, 等. 低盐度海水和淡水对中华绒螯蟹性腺发育及交配行为的影响[J]. 动物学杂志, 2013, 48(4): 555-561.
    [8]
    吴建辉, 王家启, 戴小杰, 等. 基于概率模型的长江口鱼类空间共现模式分析[J]. 南方水产科学, 2019, 15(1): 1-9. doi: 10.12131/20180112
    [9]
    ZHU G L, WANG L J, TANG W Q, et al. Identification of olfactory receptor genes in the Japanese grenadier anchovy Coilia nasus[J]. Genes Genomics, 2017, 39(5): 521-532. doi: 10.1007/s13258-017-0517-8
    [10]
    DUAN J R, ZHOU Y F, XU D P, et al. Ovary transcriptome profiling of Coilia nasus during spawning migration stages by Illumina sequencing[J]. Mar Genomics, 2015, 21: 17-19.
    [11]
    LI W X, ZOU H, WU S G, et al. Richness and diversity of helminth communities in the JapaneseI grenadier anchovy, Coilia nasus, during its anadromous migration in the Yangtze River, China[J]. J Parasitol, 2012, 98(3): 449-452. doi: 10.1645/GE-2983.1
    [12]
    LI W X, SONG R, WU S G, et al. Seasonal occurrence of helminths in the anadromous fish Coilia nasus (Engraulidae): parasite indecators of fish migratory movements[J]. J Parasitol, 2011, 97(2): 192-196. doi: 10.1645/GE-2621.1
    [13]
    ZHANG H, WU G, XIE P, et al. Role of body size and temporal hydrology in the dietary shifts of shortjaw tapertail anchovy Coi-lia brachygnathus (Actinopterygii, Engraulidae) in a large floodplain lake[J]. Hydrobiologia, 2013, 703(1): 247-256. doi: 10.1007/s10750-012-1370-z
    [14]
    代培, 严燕, 朱孝彦, 等. 长江刀鲚国家级水产种质资源保护区(安庆段)刀鲚资源现状[J]. 中国水产科学, 2020, 27(11): 1267-1276.
    [15]
    SHEN H, GU R, XU G, et al. In-depth transcriptome analysis of Coilia ectenes, an important fish resource in the Yangtze River: de novo assembly, gene annotation[J]. Mar Genomics, 2015, 23: 15-17. doi: 10.1016/j.margen.2015.03.002
    [16]
    鲜博, 高建操, 徐钢春, 等. 盐度对刀鲚生长、抗氧化应激和渗透压调节能力的影响[J]. 海洋湖沼通报, 2020(2): 152-159.
    [17]
    王武. 鱼类增养殖学[M]. 北京: 中国农业出版社, 2000: 193.
    [18]
    XU G, DU F, LI Y, et al. Integrated application of transcripto-mics and metabolomics yields insights into population-asynchronous ovary development in Coilia nasus[J]. Sci Rep, 2016, 6(1): 1-11. doi: 10.1038/s41598-016-0001-8
    [19]
    YIN D, LIN D, YING C, et al. Metabolic mechanisms of Coilia nasus in the natural food intake state during migration[J]. Genomics, 2020, 112(5): 3294-3305. doi: 10.1016/j.ygeno.2020.05.027
    [20]
    ZHAO H J, XU J K, YAN Z H, et al. Microplastics enhance the developmental toxicity of synthetic phenolic antioxidants by disturbing the thyroid function and metabolism in developing zebrafish[J]. Environ Int, 2020, 140: 105750. doi: 10.1016/j.envint.2020.105750
    [21]
    DING J, HUANG Y, LIU S, et al. Toxicological effects of nano-and micro-polystyrene plastics on red tilapia: are larger plastic particles more harmless?[J]. J Hazard Mater, 2020, 396: 122693. doi: 10.1016/j.jhazmat.2020.122693
    [22]
    ZHANG H, LIU Y, ZHOU L, et al. Metabonomic insights into the sperm activation mechanisms in ricefield eel (Monopterus albus)[J]. Genes, 2020, 11(11): 1259. doi: 10.3390/genes11111259
    [23]
    HUANG Y, ZHANG Y, ZHENG J, et al. Metabolic profiles of fish nodavirus infection in vitro: RGNNV induced and exploited cellular fatty acid synthesis for virus infection[J]. Cell Microbiol, 2020, 22(9): e13216.
    [24]
    OZEN G, NOREL X. Prostanoids in the pathophysiology of human coronary artery[J]. Prostag Oth Lipid M, 2017, 133: 20-28.
    [25]
    YAN H, ZHANG M Z, WONG G, et al. Mechanisms of U46619-induced contraction in mouse intrarenal artery[J]. Clin Exp Pharmacol Physiol, 2019, 46(7): 643-651. doi: 10.1111/1440-1681.13087
    [26]
    SMYTH E M. Thromboxane and the thromboxane receptor in cardiovascular disease[J]. Clin Lipidol, 2010, 5(2): 209-219. doi: 10.2217/clp.10.11
    [27]
    GU W, MADRID D M D, YANG G, et al. Unaltered influenza disease outcomes in swine prophylactically treated with α-galactosylceramide[J]. Dev Comp Immunol, 2021, 114: 103843. doi: 10.1016/j.dci.2020.103843
    [28]
    WANG T, LIU F, TIAN G, et al. Lineage/species-specific expansion of the Mx gene family in teleosts: differential expression and modulation of nine Mx genes in rainbow trout Oncorhynchus mykiss[J]. Fish Shellfish Immunol, 2019, 90: 413-430. doi: 10.1016/j.fsi.2019.04.303
    [29]
    HOEHN K, MARIEB E N. Human anatomy & physiology[M]. San Francisco: Benjamin Cummings, 2010: 1064-1094.
    [30]
    SOPINKA N M, CAPELLE P M, SEMENIUK C A D, et al. Glucocorticoids in fish eggs: variation, interactions with the environment, and the potential to shape offspring fitness[J]. Physiol Biochem Zool, 2017, 90(1): 15-33. doi: 10.1086/689994
    [31]
    MOUSA M A, IBRAHIM M G, KORA M F, et al. Experimental studies on the reproduction of the thin-lipped mullet, Liza ramada[J]. Egypt J Aquat Biol Fish, 2018, 22(3): 125-138. doi: 10.21608/ejabf.2018.9455
    [32]
    CUI W, MA A, WANG X, et al. Myo-inositol enhances the low-salinity tolerance of turbot (Scophthalmus maximus) by modula-ting cortisol synthesis[J]. Biochem Biophys Res Commun, 2020, 526(4): 913-919. doi: 10.1016/j.bbrc.2020.04.004
    [33]
    胡静, 叶乐, 吴开畅, 等. 急性盐度胁迫对克氏双锯鱼幼鱼血浆皮质醇浓度和 Na+-K+-ATP 酶活性的影响[J]. 南方水产科学, 2016, 12(2): 116-120. doi: 10.3969/j.issn.2095-0780.2016.02.017
    [34]
    SARAVANAN M, RAMESH M, PETKAM R, et al. Influence of environmental salinity and cortisol pretreatment on gill Na+/K+-ATPase activity and survival and growth rates in Cyprinus carpio[J]. Aquac Rep, 2018, 11: 1-7. doi: 10.1016/j.aqrep.2018.04.002
    [35]
    KWON N H, FOX P L, KIM S. Aminoacyl-tRNA synthetases as therapeutic targets[J]. Nat Rev Drug Discov, 2019, 18(8): 629-650. doi: 10.1038/s41573-019-0026-3
    [36]
    GOMEZ M A R, IBBA M. Aminoacyl-tRNA synthetases[J]. RNA, 2020, 26(8): 910-936. doi: 10.1261/rna.071720.119
    [37]
    GARAVITO M F, NARVÁEZ-ORTIZ H Y, ZIMMERMANN B H. Pyrimidine metabolism: dynamic and versatile pathways in pathogens and cellular development[J]. J Genet Genomics, 2015, 42(5): 195-205. doi: 10.1016/j.jgg.2015.04.004
    [38]
    LAVIEU G, SCARLATTI F, SALA G, et al. Regulation of autophagy by sphingosine kinase 1 and its role in cell survival during nutrient starvation[J]. J Biol Chem, 2006, 281(13): 8518-8527. doi: 10.1074/jbc.M506182200
    [39]
    HANNUN Y A, OBEID L M. The ceramide-centric universe of lipid-mediated cell regulation: stress encounters of the lipid kind[J]. J Biol Chem, 2002, 277(29): 25847-25850. doi: 10.1074/jbc.R200008200
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