Volume 18 Issue 3
Jun.  2022
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GAO Shufang, ZHANG Jinpeng, SHI Yonghai, YUAN Xincheng, LIU Qigen. Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry[J]. South China Fisheries Science, 2022, 18(3): 68-75. DOI: 10.12131/20210185
Citation: GAO Shufang, ZHANG Jinpeng, SHI Yonghai, YUAN Xincheng, LIU Qigen. Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry[J]. South China Fisheries Science, 2022, 18(3): 68-75. DOI: 10.12131/20210185
shu

Metabonomics analysis of ovaries of Coilia nasus in seawater and freshwater based on liquid chromatography-mass spectrometry

  • 1.

    Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education/Centre for Research on Environmental Ecology and Fish Nutrition, Ministry of Agriculture and Rural Affairs/Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China

  • 2.

    Shanghai Fisheries Research Institute/Shanghai Fisheries Technology Extension Station, Shanghai 200433, China

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  • Received Date: June 23, 2021
  • Revised Date: October 08, 2021
  • Accepted Date: November 03, 2021
  • Available Online: November 09, 2021
    Graphical Abstract
    • Abstract
  • In order to clarify the effects of seawater and freshwater on the ovary development of Coilia nasus, we analyzed their differences by using non-targeted metabolomics, and compared with database of KEGG directly to find out the corresponding metabolic pathways, then analyzed its causes. The results show that a total of 47 metabolites had significant difference between the two groups (P<0.05, FC>1, VIP>1). Compared with the seawater group, the most significant differences in the expression were carbocyclic thromboxane A2, Galactosylceramide, and their differences were 10.40 and 2.78 times, respectively. The cortisol in the ovarian tissue of the freshwater group increased by 1.61 times. According to the analysis on KEGG metabolic pathways of 47 different metabolites, the biosynthesis of aminoacyl-tRNA and pyridine metabolic pathways changed in the seawater and freshwater environments significantly (P<0.05). The biosynthesis pathway of cortisol, aminoyl-tRNA, pyrimidine metabolism pathway and sphingo-lipid metabolism pathway may be related to the ovarian development during the reproductive migration of C. nasus.
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