Sequencing of whole genome of Bacillus velezensis LG37 and screening of inorganic nitrogen metabolism candidate genes

It has been found that Bacillus velezensis can assimilate inorganic nitrogen efficiently. However, the underlying mechanism of inorganic nitrogen assimilation remains enigmatic. In order to elucidate the mechanism, we sequenced the complete genome of LG37 by PacBio RS II and Illumina HiSeq 2000, and then annotated and analyzed the sequence by the database of NR, KEGG, eggNOG, GO and CARD. Finally, we screened the genes related to inorganic nitrogen metabolism by local Blast+. The results show that: 1) The genome contained one circular chromosomal with a size of 3 929 697 bp and a GC-content of 46.5%. Gene prediction and annotation was performed to acquire a total of 3 854 protein-coding genes, 86 tRNA genes and 27 rRNA genes. 2) A total of 94 inorganic nitrogen metabolism candidate genes were screened by local Blast+. These genes were involved into coding sensing protein, transcriptional regulator, transporter, oxidoreductase and assimilator, etc.. In conclusion, the whole genome sequencing and data analysis of LG37 provide data at gene level and theoretical basis for functional study and application of Bacillus in reducing inorganic nitrogen in aquaculture water.