Volume 17 Issue 6
Dec.  2021
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JIA Puyuan, GUO Huayang, ZHU Kecheng, LIU Baosuo, GUO Liang, ZHANG Nan, JIANG Shigui, ZHANG Dianchang. Cryopreservation of sperm of Acanthopagrus latus[J]. South China Fisheries Science, 2021, 17(6): 58-65. DOI: 10.12131/20210083
Citation: JIA Puyuan, GUO Huayang, ZHU Kecheng, LIU Baosuo, GUO Liang, ZHANG Nan, JIANG Shigui, ZHANG Dianchang. Cryopreservation of sperm of Acanthopagrus latus[J]. South China Fisheries Science, 2021, 17(6): 58-65. DOI: 10.12131/20210083
shu

Cryopreservation of sperm of Acanthopagrus latus

  • 1.

    College of Ocean, Hebei Agricultural University, Qinhuangdao 066003, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 3.

    Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China

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  • Received Date: March 18, 2021
  • Revised Date: May 27, 2021
  • Accepted Date: June 20, 2021
  • Available Online: June 29, 2021
    Graphical Abstract
    • Abstract
  • Acanthopagrus latus is one of the important breeding species in China, whose sperm cryopreservation can provide technical supports for its breeding, prevent the decline of its germplasm resources and maintain its sustainable development of the breeding industry effectively. In this experiment, we screened the diluent, glucose concentration, antifreeze type and concentration as well as dilution ratio and cooling procedure of the spermatozoa of A. latus. The results show that when the sperm were diluted with MPRS solution containing 10 g·L−1 glucose and 5% ethylene glycol at the ratio of 1∶2, balanced at 4 ℃ for 30 min, fumigated 5 cm on the liquid nitrogen surface for 5 min, and finally stored in liquid nitrogen for 2 h, the frozen sperm had the best motility after thawing in 37 ℃ water bath, and the sperm motility, motility time and life span could reach (85.170±3.66)%, (9.16±70) s and (94.297±9.55) s, respectively.
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    • References (41)
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