Volume 17 Issue 5
Sep.  2021
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ZHANG Xueyan, QIN Xiaoming, LIN Haisheng, CAO Wenhong, ZHENG Huina, GAO Jialong, ZHANG Chaohua. Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells[J]. South China Fisheries Science, 2021, 17(5): 118-125. DOI: 10.12131/20210071
Citation: ZHANG Xueyan, QIN Xiaoming, LIN Haisheng, CAO Wenhong, ZHENG Huina, GAO Jialong, ZHANG Chaohua. Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells[J]. South China Fisheries Science, 2021, 17(5): 118-125. DOI: 10.12131/20210071
shu

Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells

  • 1.

    College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China

  • 2.

    Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution/National Research and Development Branch Center for Shellfish Processing (Zhanjiang)/South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhanjiang 524088, China

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  • Received Date: March 03, 2021
  • Revised Date: March 21, 2021
  • Accepted Date: April 27, 2021
  • Available Online: May 09, 2021
    Graphical Abstract
    • Abstract
  • In this study, TM4 Sertoli cells were injured by triptolide (TP) to construct a germ cell injury model, so as to explore the protective effect of oyster (Crassostrea hongkongensis) hydrolyzed ultrafiltration fractions on TP-induced oxidative damage of TM4 Sertoli cells in mice (Mus musculus). Besides, the molecular mass distribution and trace metal element content of the oyster hydrolyzed ultrafiltration fractions were detected, and the cell viability, GSH content, MDA content and ROS level of TM4 cells induced by TP by ultrafiltration fractions were compared. The results show that the oyster hydrolyzed ultrafiltration fractions are rich in trace metal elements such as copper, zinc, manganese and selenium; most small molecules and macromolecular substances were separated by ultrafiltration; compared with the model group, ultrafiltration fractions improved the cell viability of TM4 cells induced by TP. The cell viability of <3, 3–5 and 5–10 ku ultrafiltration fractions was higher than that of >10 ku ultrafiltration fraction; <3 ku ultrafiltration fraction could prevent TP-induced oxidative stress damage in TM4 cells, reduce the production of intracellular reactive oxygen species and lipid peroxidation, and enhance the antioxidant activity of TM4 cells.
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    • References (39)
  • [1]
    WILLEM O, IAN C, SILKE D, et al. Infertility and the provision of infertility medical services in developing countries[J]. Hum Reprod Update, 2008, 14(6): 605-621. doi: 10.1093/humupd/dmn042
    [2]
    JAROW J P, SHARLIP I D, BELKER A M, et al. Best practice policies for male infertility[J]. Fertil Steril, 2002, 77(5): 873-882. doi: 10.1016/S0015-0282(02)03105-9
    [3]
    LEE P A, HOUK C P. Cryptorchidism[J]. Curr Opin Endocrinol, 2013, 20(3): 210-216. doi: 10.1097/MED.0b013e32835ffc7d
    [4]
    LI X, YAO Z, YANG D, et al. Cyanidin-3-O-glucoside restores spermatogenic dysfunction in cadmium-exposed pubertal mice via histone ubiquitination and mitigating oxidative damage[J]. J Hazard Mater, 2019, 387: 121706.
    [5]
    BUDIN S B, KHO J H, LEE J H, et al. Low-dose nicotine exposure induced the oxidative damage of reproductive organs and altered the sperm characteristics of adolescent male rats[J]. Malays J Med Sci, 2017, 24(6): 50-57.
    [6]
    NI F D, HAO S L, YANG W X. Molecular insights into hormone regulation via signaling pathways in Sertoli cells: with discussion on infertility and testicular tumor[J]. Gene, 2020, 753: 144812. doi: 10.1016/j.gene.2020.144812
    [7]
    ZHAO Y, ZHANG P, GE W, et al. Alginate oligosaccharides improve germ cell development and testicular microenvironment to rescue busulfan disrupted spermatogenesis[J]. Theranostics, 2020, 10(7): 3308-3324. doi: 10.7150/thno.43189
    [8]
    CHEN Y, WANG J, CHEN X, et al. Microcystin-leucine arginine mediates apoptosis and engulfment of Leydig cell by testicular macrophages resulting in reduced serum testosterone levels[J]. Aquat Toxicol, 2018, 199: 116-126. doi: 10.1016/j.aquatox.2018.03.018
    [9]
    BISHT S, FAIQ M, TOLAHUNASE M, et al. Oxidative stress and male infertility[J]. Nat Rev Urol, 2017, 14(8): 470-485. doi: 10.1038/nrurol.2017.69
    [10]
    WANG B, MA L, TAO X, et al. Triptolide, an active component of the Chinese herbal remedy Tripterygium wilfordii Hook. f., inhibits production of nitric oxide by decreasing inducible nitric oxide synthase gene transcription[J]. Arthritis Rheum, 2004, 50(9): 2995-3003. doi: 10.1002/art.20459
    [11]
    徐颖, 樊媛芳, 赵元, 等. 近40年雷公藤生殖毒性研究概述[J]. 中国中药杂志, 2019, 44(16): 3406-3414.
    [12]
    HUYNH P N, HIKIM A P S, WANG C, et al. Long-term effects of triptolide on spermatogenesis, epididymal sperm function, and fertility in male rats[J]. J Androl, 2000, 21(5): 689-699.
    [13]
    WANG Y, GUO S H, SHANG X J, et al. Triptolide induces Sertoli cell apoptosis in mice via ROS/JNK-dependent activation of the mitochondrial pathway and inhibition of Nrf2-mediated antioxidant response[J]. Acta Pharmacologica Sinica, 2018, 39(2): 311-327. doi: 10.1038/aps.2017.95
    [14]
    代春美, 廖晓宇, 叶祖光. 海洋中药牡蛎的化学成分、药理活性及开发应用[J]. 天然产物研究与开发, 2016, 28(3): 471-474, 437.
    [15]
    叶知秋. 海鲜食疗养生法[M]. 长春: 吉林科学出版社, 2004: 98-100.
    [16]
    陈必链, 王绍钊, 吴松刚. 牡蛎水提取氨基酸的研究[J]. 适用技术市场, 1998(4): 25-26.
    [17]
    HAO G, CAO W, HAO J, et al. In vitro antioxidant activity and in vivo anti-fatigue effects of oyster (Ostrea plicatula Gmelin) peptides prepared using neutral proteinase[J]. Food Sci Technol Res, 2013, 19(4): 623-631. doi: 10.3136/fstr.19.623
    [18]
    李婉, 曹文红, 章超桦, 等. 牡蛎酶解产物的组成特点及其体外免疫活性[J]. 食品工业科技, 2017, 38(16): 35-42.
    [19]
    MIAO J, LIAO W, KANG M, et al. Anti-fatigue and anti-oxidant activities of oyster (Ostrea rivularis) hydrolysate prepared by compound protease[J]. Food Funct, 2018, 9(12): 6577-6585. doi: 10.1039/C8FO01879K
    [20]
    吕佳桐, 林海生, 秦小明, 等. 牡蛎及其酶解产物抗皮肤光老化的初步研究[J]. 南方水产科学, 2021, 17(1): 91-100. doi: 10.12131/20200141
    [21]
    邱娟, 沈建东, 翁凌, 等. 利用牡蛎制备ACE抑制肽的工艺优化[J]. 食品科学, 2017, 38(16): 165-172. doi: 10.7506/spkx1002-6630-201716026
    [22]
    邓燕群. 牡蛎活性肽及其复合物降血糖作用的研究[D]. 汕头: 汕头大学, 2015: 27-30.
    [23]
    ZHANG C, LI X, JING X, et al. Protective effects of oyster extract against hepatic tissue injury in alcoholic liver diseases[J]. J Ocean Univ China, 2014, 13(2): 262-270. doi: 10.1007/s11802-014-2449-0
    [24]
    张雪妍, 秦小明, 高加龙, 等. 牡蛎酶解工艺优化及其酶解产物对小鼠睾酮分泌的影响[J]. 广东海洋大学学报, 2019, 39(3): 96-102. doi: 10.3969/j.issn.1673-9159.2019.03.013
    [25]
    陈悦, 李路, 闫朝阳, 等. 小分子牡蛎多肽对雄性小鼠性功能的影响[J]. 基因组学与应用生物学, 2019, 38(1): 109-116.
    [26]
    LI S, SONG Z, LIU T, et al. Polysaccharide from Ostrea rivularis attenuates reproductive oxidative stress damage via activating Keap1-Nrf2/ARE pathway[J]. Carbohydr Polym, 2018: 321-331.
    [27]
    黄艳球. 牡蛎肉及其酶解产物对半去势雄性大鼠性功能的影响[D]. 湛江: 广东海洋大学, 2019: 12-24.
    [28]
    LI W, XU C, ZHANG C, et al. The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate[J]. RSC Adv, 2019, 9(56): 32854-32863. doi: 10.1039/C9RA04255E
    [29]
    MA B, ZHANG J, ZHU Z M, et al. Aucubin, a natural iridoid glucoside, attenuates oxidative stress-induced testis injury by inhibiting JNK and CHOP activation via Nrf2 up-regulation[J]. Phytomedicine, 2019, 64: 153057. doi: 10.1016/j.phymed.2019.153057
    [30]
    MA B, ZHANG J, ZHU Z M, et al. Luteolin ameliorates testis injury and blood-testis barrier disruption through the Nrf2 signaling pathway and by upregulating Cx43[J]. Mol Nutr Food Res, 2019, 63(10): e1800843. doi: 10.1002/mnfr.201800843
    [31]
    SKORACKA K, EDER P, DOBROWOLSKA A. Diet and nutritional factors in male (in) fertility: underestimated factors[J]. J Clin Med, 2020, 9(5): 1400. doi: 10.3390/jcm9051400
    [32]
    林海生, 秦小明, 章超桦, 等. 中国沿海主要牡蛎养殖品种的营养品质和风味特征比较分析[J]. 南方水产科学, 2019, 15(2): 110-120. doi: 10.12131/20180226
    [33]
    CHEN D, LIU Z, HUANG W, et al. Purification and characterisation of a zinc-binding peptide from oyster protein hydrolysate[J]. J Func Food, 2013, 5(2): 689-697. doi: 10.1016/j.jff.2013.01.012
    [34]
    ZHOU C, HU J, MA H, et al. Antioxidant peptides from corn gluten meal: orthogonal design evaluation[J]. Food Chem, 2015, 187(15):270-278.
    [35]
    SADIK N A H. Effects of diallyl sulfide and zinc on testicular steroidogenesis in cadmium-treated male rats[J]. J Biochem Mol Toxicol, 2010, 22(5): 345-353.
    [36]
    POURHASSANALI N, ROSHAN-MILANI S, KHERADMAND F, et al. Zinc attenuates ethanol-induced Sertoli cell toxicity and apoptosis through caspase-3 mediated pathways[J]. Reprod Toxicol, 2016, 61: 97-103. doi: 10.1016/j.reprotox.2016.03.041
    [37]
    刘姝, 王淑军, 房耀维, 等. 发酵法制备太平洋牡蛎水解液的功能特性及其抗氧化活性[J]. 水产科学, 2013, 32(11): 641-645. doi: 10.3969/j.issn.1003-1111.2013.11.003
    [38]
    林海生. 牡蛎蛋白肽的酶法制备及其改善小鼠学习记忆功能的研究[D]. 湛江: 广东海洋大学, 2013: 25-32.
    [39]
    MORIMOTO H, IWATA K, OGONUKI N, et al. ROS are required for mouse spermatogonial stem cell self-renewal[J]. Cell Stem Cell, 2013, 12(6): 774-786. doi: 10.1016/j.stem.2013.04.001
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