ZHANG Xueyan, QIN Xiaoming, LIN Haisheng, CAO Wenhong, ZHENG Huina, GAO Jialong, ZHANG Chaohua. Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells[J]. South China Fisheries Science, 2021, 17(5): 118-125. DOI: 10.12131/20210071
Citation: ZHANG Xueyan, QIN Xiaoming, LIN Haisheng, CAO Wenhong, ZHENG Huina, GAO Jialong, ZHANG Chaohua. Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells[J]. South China Fisheries Science, 2021, 17(5): 118-125. DOI: 10.12131/20210071

Protective effect of hydrolyzed ultrafiltration fractions from oyster (Crassostrea hongkongensis) on oxidative damage of TM4 Sertoli cells

More Information
  • Received Date: March 03, 2021
  • Revised Date: March 21, 2021
  • Accepted Date: April 27, 2021
  • Available Online: May 09, 2021
  • In this study, TM4 Sertoli cells were injured by triptolide (TP) to construct a germ cell injury model, so as to explore the protective effect of oyster (Crassostrea hongkongensis) hydrolyzed ultrafiltration fractions on TP-induced oxidative damage of TM4 Sertoli cells in mice (Mus musculus). Besides, the molecular mass distribution and trace metal element content of the oyster hydrolyzed ultrafiltration fractions were detected, and the cell viability, GSH content, MDA content and ROS level of TM4 cells induced by TP by ultrafiltration fractions were compared. The results show that the oyster hydrolyzed ultrafiltration fractions are rich in trace metal elements such as copper, zinc, manganese and selenium; most small molecules and macromolecular substances were separated by ultrafiltration; compared with the model group, ultrafiltration fractions improved the cell viability of TM4 cells induced by TP. The cell viability of <3, 3–5 and 5–10 ku ultrafiltration fractions was higher than that of >10 ku ultrafiltration fraction; <3 ku ultrafiltration fraction could prevent TP-induced oxidative stress damage in TM4 cells, reduce the production of intracellular reactive oxygen species and lipid peroxidation, and enhance the antioxidant activity of TM4 cells.
  • [1]
    WILLEM O, IAN C, SILKE D, et al. Infertility and the provision of infertility medical services in developing countries[J]. Hum Reprod Update, 2008, 14(6): 605-621. doi: 10.1093/humupd/dmn042
    [2]
    JAROW J P, SHARLIP I D, BELKER A M, et al. Best practice policies for male infertility[J]. Fertil Steril, 2002, 77(5): 873-882. doi: 10.1016/S0015-0282(02)03105-9
    [3]
    LEE P A, HOUK C P. Cryptorchidism[J]. Curr Opin Endocrinol, 2013, 20(3): 210-216. doi: 10.1097/MED.0b013e32835ffc7d
    [4]
    LI X, YAO Z, YANG D, et al. Cyanidin-3-O-glucoside restores spermatogenic dysfunction in cadmium-exposed pubertal mice via histone ubiquitination and mitigating oxidative damage[J]. J Hazard Mater, 2019, 387: 121706.
    [5]
    BUDIN S B, KHO J H, LEE J H, et al. Low-dose nicotine exposure induced the oxidative damage of reproductive organs and altered the sperm characteristics of adolescent male rats[J]. Malays J Med Sci, 2017, 24(6): 50-57.
    [6]
    NI F D, HAO S L, YANG W X. Molecular insights into hormone regulation via signaling pathways in Sertoli cells: with discussion on infertility and testicular tumor[J]. Gene, 2020, 753: 144812. doi: 10.1016/j.gene.2020.144812
    [7]
    ZHAO Y, ZHANG P, GE W, et al. Alginate oligosaccharides improve germ cell development and testicular microenvironment to rescue busulfan disrupted spermatogenesis[J]. Theranostics, 2020, 10(7): 3308-3324. doi: 10.7150/thno.43189
    [8]
    CHEN Y, WANG J, CHEN X, et al. Microcystin-leucine arginine mediates apoptosis and engulfment of Leydig cell by testicular macrophages resulting in reduced serum testosterone levels[J]. Aquat Toxicol, 2018, 199: 116-126. doi: 10.1016/j.aquatox.2018.03.018
    [9]
    BISHT S, FAIQ M, TOLAHUNASE M, et al. Oxidative stress and male infertility[J]. Nat Rev Urol, 2017, 14(8): 470-485. doi: 10.1038/nrurol.2017.69
    [10]
    WANG B, MA L, TAO X, et al. Triptolide, an active component of the Chinese herbal remedy Tripterygium wilfordii Hook. f., inhibits production of nitric oxide by decreasing inducible nitric oxide synthase gene transcription[J]. Arthritis Rheum, 2004, 50(9): 2995-3003. doi: 10.1002/art.20459
    [11]
    徐颖, 樊媛芳, 赵元, 等. 近40年雷公藤生殖毒性研究概述[J]. 中国中药杂志, 2019, 44(16): 3406-3414.
    [12]
    HUYNH P N, HIKIM A P S, WANG C, et al. Long-term effects of triptolide on spermatogenesis, epididymal sperm function, and fertility in male rats[J]. J Androl, 2000, 21(5): 689-699.
    [13]
    WANG Y, GUO S H, SHANG X J, et al. Triptolide induces Sertoli cell apoptosis in mice via ROS/JNK-dependent activation of the mitochondrial pathway and inhibition of Nrf2-mediated antioxidant response[J]. Acta Pharmacologica Sinica, 2018, 39(2): 311-327. doi: 10.1038/aps.2017.95
    [14]
    代春美, 廖晓宇, 叶祖光. 海洋中药牡蛎的化学成分、药理活性及开发应用[J]. 天然产物研究与开发, 2016, 28(3): 471-474, 437.
    [15]
    叶知秋. 海鲜食疗养生法[M]. 长春: 吉林科学出版社, 2004: 98-100.
    [16]
    陈必链, 王绍钊, 吴松刚. 牡蛎水提取氨基酸的研究[J]. 适用技术市场, 1998(4): 25-26.
    [17]
    HAO G, CAO W, HAO J, et al. In vitro antioxidant activity and in vivo anti-fatigue effects of oyster (Ostrea plicatula Gmelin) peptides prepared using neutral proteinase[J]. Food Sci Technol Res, 2013, 19(4): 623-631. doi: 10.3136/fstr.19.623
    [18]
    李婉, 曹文红, 章超桦, 等. 牡蛎酶解产物的组成特点及其体外免疫活性[J]. 食品工业科技, 2017, 38(16): 35-42.
    [19]
    MIAO J, LIAO W, KANG M, et al. Anti-fatigue and anti-oxidant activities of oyster (Ostrea rivularis) hydrolysate prepared by compound protease[J]. Food Funct, 2018, 9(12): 6577-6585. doi: 10.1039/C8FO01879K
    [20]
    吕佳桐, 林海生, 秦小明, 等. 牡蛎及其酶解产物抗皮肤光老化的初步研究[J]. 南方水产科学, 2021, 17(1): 91-100. doi: 10.12131/20200141
    [21]
    邱娟, 沈建东, 翁凌, 等. 利用牡蛎制备ACE抑制肽的工艺优化[J]. 食品科学, 2017, 38(16): 165-172. doi: 10.7506/spkx1002-6630-201716026
    [22]
    邓燕群. 牡蛎活性肽及其复合物降血糖作用的研究[D]. 汕头: 汕头大学, 2015: 27-30.
    [23]
    ZHANG C, LI X, JING X, et al. Protective effects of oyster extract against hepatic tissue injury in alcoholic liver diseases[J]. J Ocean Univ China, 2014, 13(2): 262-270. doi: 10.1007/s11802-014-2449-0
    [24]
    张雪妍, 秦小明, 高加龙, 等. 牡蛎酶解工艺优化及其酶解产物对小鼠睾酮分泌的影响[J]. 广东海洋大学学报, 2019, 39(3): 96-102. doi: 10.3969/j.issn.1673-9159.2019.03.013
    [25]
    陈悦, 李路, 闫朝阳, 等. 小分子牡蛎多肽对雄性小鼠性功能的影响[J]. 基因组学与应用生物学, 2019, 38(1): 109-116.
    [26]
    LI S, SONG Z, LIU T, et al. Polysaccharide from Ostrea rivularis attenuates reproductive oxidative stress damage via activating Keap1-Nrf2/ARE pathway[J]. Carbohydr Polym, 2018: 321-331.
    [27]
    黄艳球. 牡蛎肉及其酶解产物对半去势雄性大鼠性功能的影响[D]. 湛江: 广东海洋大学, 2019: 12-24.
    [28]
    LI W, XU C, ZHANG C, et al. The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate[J]. RSC Adv, 2019, 9(56): 32854-32863. doi: 10.1039/C9RA04255E
    [29]
    MA B, ZHANG J, ZHU Z M, et al. Aucubin, a natural iridoid glucoside, attenuates oxidative stress-induced testis injury by inhibiting JNK and CHOP activation via Nrf2 up-regulation[J]. Phytomedicine, 2019, 64: 153057. doi: 10.1016/j.phymed.2019.153057
    [30]
    MA B, ZHANG J, ZHU Z M, et al. Luteolin ameliorates testis injury and blood-testis barrier disruption through the Nrf2 signaling pathway and by upregulating Cx43[J]. Mol Nutr Food Res, 2019, 63(10): e1800843. doi: 10.1002/mnfr.201800843
    [31]
    SKORACKA K, EDER P, DOBROWOLSKA A. Diet and nutritional factors in male (in) fertility: underestimated factors[J]. J Clin Med, 2020, 9(5): 1400. doi: 10.3390/jcm9051400
    [32]
    林海生, 秦小明, 章超桦, 等. 中国沿海主要牡蛎养殖品种的营养品质和风味特征比较分析[J]. 南方水产科学, 2019, 15(2): 110-120. doi: 10.12131/20180226
    [33]
    CHEN D, LIU Z, HUANG W, et al. Purification and characterisation of a zinc-binding peptide from oyster protein hydrolysate[J]. J Func Food, 2013, 5(2): 689-697. doi: 10.1016/j.jff.2013.01.012
    [34]
    ZHOU C, HU J, MA H, et al. Antioxidant peptides from corn gluten meal: orthogonal design evaluation[J]. Food Chem, 2015, 187(15):270-278.
    [35]
    SADIK N A H. Effects of diallyl sulfide and zinc on testicular steroidogenesis in cadmium-treated male rats[J]. J Biochem Mol Toxicol, 2010, 22(5): 345-353.
    [36]
    POURHASSANALI N, ROSHAN-MILANI S, KHERADMAND F, et al. Zinc attenuates ethanol-induced Sertoli cell toxicity and apoptosis through caspase-3 mediated pathways[J]. Reprod Toxicol, 2016, 61: 97-103. doi: 10.1016/j.reprotox.2016.03.041
    [37]
    刘姝, 王淑军, 房耀维, 等. 发酵法制备太平洋牡蛎水解液的功能特性及其抗氧化活性[J]. 水产科学, 2013, 32(11): 641-645. doi: 10.3969/j.issn.1003-1111.2013.11.003
    [38]
    林海生. 牡蛎蛋白肽的酶法制备及其改善小鼠学习记忆功能的研究[D]. 湛江: 广东海洋大学, 2013: 25-32.
    [39]
    MORIMOTO H, IWATA K, OGONUKI N, et al. ROS are required for mouse spermatogonial stem cell self-renewal[J]. Cell Stem Cell, 2013, 12(6): 774-786. doi: 10.1016/j.stem.2013.04.001
  • Related Articles

    [1]XU Bo, YUAN Hongchun. Research on fish feeding intensity classification model based on axial feature calibration and temporal segment network[J]. South China Fisheries Science, 2024, 20(6): 145-154. DOI: 10.12131/20240200
    [2]QIAN Cheng, ZHANG Jiapeng, TU Xueying, LIU Huang, QIAO Gan, LIU Shijing. Turbot fish egg recognition and counting method based on CBAM-UNet[J]. South China Fisheries Science, 2024, 20(6): 132-144. DOI: 10.12131/20240123
    [3]ZHANG Hongjiao, ZHANG Cunxi, WANG Rui, WANG Ke, QIAO Qian. Freshness recognition of small yellow croaker based on image processing and improved DenseNet network[J]. South China Fisheries Science, 2024, 20(3): 133-142. DOI: 10.12131/20230241
    [4]GONG Yuanjin, YANG Shujie. Dynamic water surface environment perception SLAM algorithm based on visual semantics and point-line fusion for unmanned surface vessels[J]. South China Fisheries Science, 2024, 20(3): 120-132. DOI: 10.12131/20240019
    [5]YUAN Yongming, SHI Pei. Study on fish movement monitoring method based on image processing[J]. South China Fisheries Science, 2018, 14(5): 109-114. DOI: 10.3969/j.issn.2095-0780.2018.05.014
    [6]LI Laihao, SUN Bolun, ZHAO Donghao. Research progress in detection and preparation methods for tetrodotoxin[J]. South China Fisheries Science, 2018, 14(3): 126-132. DOI: 10.3969/j.issn.2095-0780.2018.03.016
    [7]TANG Xianming, WANG Yingrui, LIU Cui, CHEN Fuxiao, YIN Yi, LIU Tao. Prelimary study of segment culture of Kappaphycus alvarezii[J]. South China Fisheries Science, 2014, 10(2): 36-41. DOI: 10.3969/j.issn.2095-0780.2014.02.005
    [8]HUANG Chunli, HUANG He, LIU Wenxia, GAO Ping, HUANG Guofang, LI Zhiqing, CHENG Hong, LUO Lin. Research progress on residual toxicity and detection methods of melamine[J]. South China Fisheries Science, 2011, 7(3): 76-80. DOI: 10.3969/j.issn.2095-0780.2011.03.014
    [9]ZHAO Fei, ZOU Weimin. Application of LAMP in the rapid detection of aquatic animal pathogens[J]. South China Fisheries Science, 2007, 3(2): 71-75.
    [10]WANG Li, LIN Hong, CAO Limin. Detection of aminoglycosides in foods of animal origin[J]. South China Fisheries Science, 2006, 2(1): 76-79.
  • Cited by

    Periodical cited type(6)

    1. 徐菲,徐开达,张洪亮,卢占晖,周永东,李羽如,叶莹莹,马文静,金梓慧. 浙江岱衢洋海域春秋季游泳动物群落结构及生物量粒径谱特征. 海洋与湖沼. 2025(01): 165-174 .
    2. Zhisen LUO,Murong YI,Xiaodong YANG,Xiao CHEN,Jinxi WANG,Changping JIANG,Fengming LIU,Konglan LUO,Xiongbo HE,Hung-Du LIN,Bin KANG,Yunrong YAN. Checklist of marine fishes in the Beibu Gulf: fish classification, resource protection, and biodiversity challenge. Journal of Oceanology and Limnology. 2025(01): 232-247 .
    3. 李诗佳,施利燕,钟俊生,赵路路. 长江口南部水域春、秋季鱼类群落结构比较. 上海海洋大学学报. 2024(01): 135-149 .
    4. 李淼,许友伟,孙铭帅,范江涛,李佳俊,张魁,陈作志. 拉尼娜事件前后北部湾鱼类群落结构变化研究. 南方水产科学. 2023(02): 1-11 . 本站查看
    5. Chenyu Song,Zhong Tu,Na Song. Discordant patterns of genetic variation between mitochondrial and microsatellite markers in Acanthogobius ommaturus across the coastal areas of China. Acta Oceanologica Sinica. 2023(04): 72-80 .
    6. 王鑫,李昌文,徐加涛,李士虎,宋可心,唐佳伟,马晓娜,冯志华. 灌河口海域水生动物群落结构及分布特征. 江苏海洋大学学报(自然科学版). 2023(04): 29-41 .

    Other cited types(7)

Catalog

    Article views (882) PDF downloads (41) Cited by(13)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return