Volume 17 Issue 6
Dec.  2021
Turn off MathJax
Article Contents
Abstract
References
Related Articles
WANG Nan, YIN Jiyuan, WANG Yingying, LI Yingying, WU Siyu, SHI Cunbin, LI Jiahao, CAO Jizhen, WANG Qing. Isolation, identification and biological characteristics of lactobacillus from grass carp[J]. South China Fisheries Science, 2021, 17(6): 74-84. DOI: 10.12131/20210039
Citation: WANG Nan, YIN Jiyuan, WANG Yingying, LI Yingying, WU Siyu, SHI Cunbin, LI Jiahao, CAO Jizhen, WANG Qing. Isolation, identification and biological characteristics of lactobacillus from grass carp[J]. South China Fisheries Science, 2021, 17(6): 74-84. DOI: 10.12131/20210039
shu

Isolation, identification and biological characteristics of lactobacillus from grass carp

  • 1.

    Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306, China

  • 2.

    Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs/Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Guangzhou 510380, China

More Information
  • Received Date: January 18, 2021
  • Revised Date: April 27, 2021
  • Accepted Date: May 11, 2021
  • Available Online: June 04, 2021
    Graphical Abstract
    • Abstract
  • As the problem of aquaculture diseases is growing, the harm of using antibiotics and drugs is more and more serious so the research on ecological control technology is an important way to achieve healthy aquaculture. In this study, eight lactobacillus strains were isolated from the intestinal tract of healthy grass carps (Ctenopharyngodon idella). The identification result indicates that all the eight strains were Lactobacillus plantarum. The results of biological characteristics evaluation show that L. plantarum strain Y190430 had a high growth rate, and it was sensitive to common antibiotics. In addition, compared with the standard strain and commercial lactobacillus, L. plantarum Y190430 represented better tolerance to environmental stress such as acid, alkali, salt and temperature. Besides, it had a higher acid production rate and inhibited the growth of pathogenic bacterias better. The results provide references for the research on ecological control of aquatic diseases.
    • FullText(HTML)
    • References (57)
  • [1]
    WANG P, JI J, ZHANG Y. Aquaculture extension system in China: development, challenges, and prospects[J]. Aquacult Rep, 2020, 17: 1-9.
    [2]
    农业农村部渔业渔政管理局. 2020中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2020: 1, 24.
    [3]
    朱文根, 李星浩, 饶刘瑜, 等. 感染草鱼呼肠孤病毒对肠道菌群多样性的影响[J]. 水生生物学报, 2019, 43(1): 109-116. doi: 10.7541/2019.014
    [4]
    祖国掌, 余为一, 李槿年, 等. 草鱼细菌性败血症的诊断及流行病学调查[J]. 淡水渔业, 2000, 30(5): 35-37. doi: 10.3969/j.issn.1000-6907.2000.05.017
    [5]
    BRUDESETH B E, WIULSRØD R, FREDRIKSEN B N, et al. Status and future perspectives of vaccines for industrialised fin-fish farming[J]. Fish Shellfish Immunol, 2013, 35(6): 1759-1768. doi: 10.1016/j.fsi.2013.05.029
    [6]
    WANG Q C, JI W, XU Z. Current use and development of fish vaccines in China[J]. Fish Shellfish Immunol, 2020, 96: 223-234. doi: 10.1016/j.fsi.2019.12.010
    [7]
    马丽, 吴金英, 高凇泽, 等. 海豚链球菌simA和pgmA真核表达质粒对尼罗罗非鱼免疫保护的研究[J]. 南方水产科学, 2020, 16(3): 38-46. doi: 10.12131/20190163
    [8]
    徐吟梅. 首个草鱼出血病活疫水产疫苗成功问世[J]. 现代渔业信息, 2011, 26(3): 73-74.
    [9]
    巩华. 水产疫苗添新军——嗜水气单胞菌败血症灭活疫苗获生产批文[J]. 海洋与渔业, 2012(3): 49-49. doi: 10.3969/j.issn.1672-4046.2012.03.045
    [10]
    钱冬, 陈月英, 沈锦玉, 等. 引起鱼类暴发性流行病的嗜水气单胞菌的血清型, 毒力及溶血性[J]. 微生物学报, 1995, 35(6): 460-464.
    [11]
    魏文娟, 赵姝, 王元, 等. 副溶血弧菌养殖对虾分离株耐药性及耐药基因分析[J]. 南方水产科学, 2020, 16(1): 9-16. doi: 10.12131/20190165
    [12]
    郭秀平, 刘毅辉, 潘厚军, 等. 我国水产药物残留与休药期研究现状概述[J]. 海洋与渔业, 2018(11): 76-78.
    [13]
    ZIELIŃSKA D, DŁUGOSZ E, ZAWISTOWSKA-DENIZIAK A. Functional properties of food origin lactobacillus in the gastrointestinal ecosystem: in vitro study[J]. Probiotics Antimicrob Proteins, 2019, 11(3): 820-829. doi: 10.1007/s12602-018-9458-z
    [14]
    HYRONIMUS B, LE MARREC C, SASSI A H, et al. Acid and bile tolerance of spore-forming lactic acid bacteria[J]. Int J Food Microbiol, 2000, 61(2/3): 193-197.
    [15]
    MIKULSKI D, JANKOWSKI J, MIKULSKA M, et al. Effects of dietary probiotic (Pediococcus acidilactici) supplementation on productive performance, egg quality, and body composition in laying hens fed diets varying in energy density[J]. Poult Sci, 2020, 99(4): 2275-2285. doi: 10.1016/j.psj.2019.11.046
    [16]
    KEWCHAROEN W, SRISAPOOME P. Probiotic effects of Bacillus spp. from Pacific white shrimp (Litopenaeus vannamei) on water quality and shrimp growth, immune responses, and resistance to Vibrio parahaemolyticus (AHPND strains)[J]. Fish Shellfish Immunol, 2019, 94: 175-189.
    [17]
    CHEN C C, LI J Y, ZHANG H X, et al. Effects of a probiotic on the growth performance, intestinal flora, and immune function of chicks infected with Salmonella pullorum[J]. Poult Sci, 2020, 99(11): 5316-5323. doi: 10.1016/j.psj.2020.07.017
    [18]
    陈凯, 朱璐丹, 谭宏亮, 等. 2株乳酸菌抑菌作用研究及安全性评价[J]. 南方水产科学, 2019, 15(5): 118-125. doi: 10.12131/20190012
    [19]
    FENG Z F, SONG X J, ZHAO L T, et al. Isolation of probiotics and their effects on growth, antioxidant and non-specific immunity of sea cucumber Apostichopus japonicus[J]. Fish Shellfish Immunol, 2020, 106: 1087-1094. doi: 10.1016/j.fsi.2020.08.049
    [20]
    BENBARA T, LALOUCHE S, DRIDER D et al. Lactobacillus plantarum S27 from chicken faeces as a potential probiotic to replace antibiotics: evidence[J]. Benef Microbes, 2020, 11(2): 163-173. doi: 10.3920/BM2019.0116
    [21]
    布坎南 R E, 吉本斯 N E. 伯杰细菌鉴定手册[M]. 中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组, 译. 北京: 科学出版社, 1984: 809.
    [22]
    YIN J Y, WANG Q, WANG Y Y, et al. Development of a simple and rapid reverse transcription-loopmediated isothermal amplification (RT-LAMP) assay for sensitive detection of tilapia lake virus[J]. J Fish Dis, 2019, 42(26): 817-824.
    [23]
    高艳侠, 张德锋, 可小丽, 等. 罗非鱼源无乳链球菌肠道拮抗芽孢杆菌的筛选及其生物学特性[J]. 微生物学报, 2019, 59(5): 926-938.
    [24]
    农村农业部渔业渔政管理局, 全国水产技术推广总站. 2020中国水生动物卫生状况报告[R]. 北京: 中国农业出版社, 2020: 3.
    [25]
    蒋魁, 徐力文, 苏友禄, 等. 2012年~2014年南海海水养殖鱼类病原菌哈维弧菌分离株的耐药性分析[J]. 南方水产科学, 2016, 12(6): 99-107. doi: 10.3969/j.issn.2095-0780.2016.06.013
    [26]
    朱世超, 钱卓真, 吴成业. 水产品中7种大环内酯类抗生素残留量的测定HPLC-MS/MS法[J]. 南方水产科学, 2012, 8(1): 54-60. doi: 10.3969/j.issn.2095-0780.2012.01.009
    [27]
    WANG Z, WANG L, CHEN Z, et al. In vitro evaluation of swine-derived Lactobacillus reuteri: probiotic properties and effects on intestinal porcine epithelial cells challenged with Enterotoxigenic Escherichia coli K88[J]. J Microbiol Biotechnol, 2016, 26(6): 1018-1025. doi: 10.4014/jmb.1510.10089
    [28]
    MOHAMMADIAN T, NASIRPOUR M, TABANDEH M R, et al. Administrations of autochthonous probiotics altered juvenile rainbow trout Oncorhynchus mykiss health status, growth performance and resistance to Lactococcus garvieae, an experimental infection[J]. Fish Shellfish Immunol, 2019, 86: 269-279. doi: 10.1016/j.fsi.2018.11.052
    [29]
    SHA Y J, WANG L, LIU M, et al. Effects of lactic acid bacteria and the corresponding supernatant on the survival, growth performance, immune response and disease resistance of Litopenaeus vannamei[J]. Aquaculture, 2016, 452: 28-36. doi: 10.1016/j.aquaculture.2015.10.014
    [30]
    LI C, REN Y C, JIANG S H, et al. Effects of dietary supplementation of four strains of lactic acid bacteria on growth, immune-related response and genes expression of the juvenile sea cucumber Apostichopus japonicus Selenka[J]. Fish Shellfish Immunol, 2018, 74: 69-75. doi: 10.1016/j.fsi.2017.12.037
    [31]
    De MELO P V, de OLIVEIRA C B, JÚNIOR A M, et al. How to select a probiotic? A review and update of methods and criteria[J]. Biotechnol Adv, 2018, 36(8): 2060-2076. doi: 10.1016/j.biotechadv.2018.09.003
    [32]
    MAN L L, XIANG D J. Characterization of a broad spectrum bacteriocin produced by Lactobacillus plantarum MXG-68 from inner Mongolia traditional fermented koumiss[J]. Folia Microbiol (Praha), 2019, 64(6): 821-834. doi: 10.1007/s12223-019-00697-0
    [33]
    王水泉, 包艳, 董喜梅, 等. 植物乳杆菌的生理功能及应用[J]. 中国农业科技导报, 2010, 12(4): 49-55. doi: 10.3969/j.issn.1008-0864.2010.04.10
    [34]
    刘佳琪, 高帅, 段可馨, 等. 鱼源植物乳杆菌表达IPNV VP2-VP3重组蛋白及其口服免疫程序[J]. 水产学报, 2017, 41(4): 622-627.
    [35]
    NEWAJ F A, AL-HARBI A H, AUSTIN B. Review: developments in the use of probiotics for disease control in aquaculture[J]. Aquaculture, 2014, 431: 1-11. doi: 10.1016/j.aquaculture.2013.08.026
    [36]
    CANDELA M, PERNA F, CARNEVALI P, et al. Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells: adhesion properties, competition against enteropathogens and modulation of IL-8 production[J]. Int J Food Microbiol, 2008, 125(3): 286-292. doi: 10.1016/j.ijfoodmicro.2008.04.012
    [37]
    FRAQUEZA M J. Antibiotic resistance of lactic acid bacteria isolated from dry-fermented sausages[J]. Int J Food Microbiol, 2015, 212(SI): 76-88.
    [38]
    阚刘刚, 赵丽杰, 李秀业, 等. 鸡沙门氏菌病的生物预防和控制研究进展[J]. 动物营养学报, 2018, 30(9): 3432-3443. doi: 10.3969/j.issn.1006-267x.2018.09.012
    [39]
    杨红玲, 孙云章, 叶继丹, 等. 2株鱼源乳酸菌的生物学特性研究[J]. 西北农林科技大学学报 (自然科学版), 2008, 36(8): 25-30.
    [40]
    杨媛媛, 王楠楠, 曹青, 等. 鲫肠道乳酸菌的分离及益生特性[J]. 水产学报, 2018, 42(10): 1596-1605.
    [41]
    赵芳, 李艳琴, 李彬春. 模拟人体胃肠道环境筛选益生乳杆菌[J]. 微生物学通报, 2016, 43(6): 1396-1403.
    [42]
    龙华. 温度对鱼类生存的影响[J]. 中山大学学报 (自然科学版), 2005, 44(S1): 254-257.
    [43]
    KAKELAR H M, BARZEGARI A, HANIFIAN S, et al. Isolation and molecular identification of Lactobacillus with probiotic potential from abomasums driven rennet[J]. Food Chem, 2019, 272: 709-714. doi: 10.1016/j.foodchem.2018.08.081
    [44]
    GHOLIZADEH P, MAHALLEI M, PORMOHAMMAD, et al. Microbial balance in the intestinal normal microbiome and its association with diabetes, obesity and allergic disease[J]. Microb Pathogenesis, 2019, 127: 48-55. doi: 10.1016/j.micpath.2018.11.031
    [45]
    KIYMACI M E, ALTANLAR N, GUMUSTAS M A, et al. Quorum sensing signals and related virulenc inhibition of Pseudomo nasaeruginosa by a potential probiotic strain's organic acid[J]. Microb Pathogenesis, 2018, 121: 190-197. doi: 10.1016/j.micpath.2018.05.042
    [46]
    WANG A R, RAN C, WANG Y B, et al. Use of probiotics in aquaculture of China: a review of the past decade[J]. Fish Shellfish Immunol, 2019, 86: 734-755. doi: 10.1016/j.fsi.2018.12.026
    [47]
    CAI Y, BENNO Y, NAKASE T, et al. Specific probiotic characterization of Weissella hellenica DS-12 isolated from flounder intestine[J]. J Gen Appl Microbiol, 1998, 44(5): 311-316. doi: 10.2323/jgam.44.311
    [48]
    WANG Y G, LEE K L, NAJIAH M, et al. A new bacterial white spot syndrome (BWSS) in cultured tiger shrimp Penaeus monodon and its comparison with white spot syndrome (WSS) caused by virus[J]. Dis Aquat Organ, 2000, 41(1): 9-18.
    [49]
    骆艺文, 郝志凯, 王印庚, 等. 一株引起刺参“腐皮综合征”的蜡样芽胞杆菌[J]. 水产科技情报, 2009, 36(2): 60-63. doi: 10.3969/j.issn.1001-1994.2009.02.011
    [50]
    VELMURUGAN S, PALANIKUMAR P, VELAYUTHANI P, et al. Bacterial white patch disease caused by Bacillus cereus, a new emerging disease in semi-intensive culture of Litopenaeus vannamei[J]. Aquaculture, 2015, 444(3): 49-54.
    [51]
    闫肃, 李慧敏, 张晓冬, 等. 不同食物来源乳酸细菌的安全性评价[J]. 食品与发酵工业, 2018, 44(5): 82-89.
    [52]
    李绍戊, 王荻, 刘红柏, 等. 鱼源嗜水气单胞菌多重耐药菌株整合子的分子特征[J]. 中国水产科学, 2013, 20(5): 1015-1022.
    [53]
    王冉, 刘铁铮, 王恬. 抗生素在环境中的转归及其生态毒性[J]. 生态学报, 2006, 26(1): 265-270. doi: 10.3321/j.issn:1000-0933.2006.01.032
    [54]
    乔毅. 江苏省沿海地区水产养殖主要致病菌耐药性研究[D]. 上海: 上海海洋大学, 2015: 1-96.
    [55]
    AQUILANTI L, GAROFALO C, OSIMANI A A, et al. Isolation and molecular characterization of anti biotic-resistant lactic acid bacteria from poultry and swine meat products[J]. J Food Protect, 2007, 70(3): 557-565. doi: 10.4315/0362-028X-70.3.557
    [56]
    ROJO-BEZARES B, SÁENZ Y, POETA P A, et al. Assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine[J]. Int J Food Microbiol, 2006, 111(3): 234-240. doi: 10.1016/j.ijfoodmicro.2006.06.007
    [57]
    GEVERS D, HUYS G, SWINGS J. In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other gram positive bacteria[J]. FEMS Microbiol Lett, 2003, 225(1): 125-130. doi: 10.1016/S0378-1097(03)00505-6
    • Related Articles

  • Related Articles

    [1]HU Xiaojuan, YANG Keng, WEN Guoliang, SU Haochang, XU Yunna, XU Chuangwen, XU Yu, XU Wujie, CAO Yucheng. Algicidal effect of bacteria CZBC1 on Microcystis aeruginosa in chloride type saline-alkali water[J]. South China Fisheries Science, 2024, 20(5): 169-175. DOI: 10.12131/20240142
    [2]Cover[J]. South China Fisheries Science, 2023, 19(4).
    [3]Cover[J]. South China Fisheries Science, 2023, 19(3).
    [4]cover[J]. South China Fisheries Science, 2022, 18(6).
    [5]cover[J]. South China Fisheries Science, 2022, 18(5).
    [6]cover[J]. South China Fisheries Science, 2022, 18(4).
    [7]cover[J]. South China Fisheries Science, 2022, 18(3).
    [8]WANG Hewei, ZHANG Zhe, MA Shengwei, CHEN Haigang, HUANG Zhifei, GONG Xiuyu, CAI Wengui, JIA Xiaoping. Effect of perfluorooctane sulfonate potassium on glutathione content and glutathione S-transferase activity of red sea bream[J]. South China Fisheries Science, 2012, 8(4): 23-28. DOI: 10.3969/j.issn.2095-0780.2012.04.004
    [9]CENG Shaokui, YANG Ping, CHEN Xiuhong. Study on the removal of fish odour and bitter from protein hydrolysates of tilapia by-products by microorganism fermentation[J]. South China Fisheries Science, 2009, 5(4): 58-63. DOI: 10.3969/j.issn.1673-2227.2009.04.011
    [10]CHEN Haigang, MA Shengwei, LIN Qin, GAN Juli, CAI Wengui, JIA Xiaoping. Effects of tributyltin chloride (TBTCl) on SOD activities, MDA contents and GPx activities in gill and liver of the black porgy (Sparus macrocephalus)[J]. South China Fisheries Science, 2009, 5(2): 23-27. DOI: 10.3969/j.issn.1673-2227.2009.02.004

  • Cited by

    Periodical cited type(3)

    1. 万树杰，陈新军. 基于机器学习的西南印度洋深海散射层声学资源密度预测. 上海海洋大学学报. 2024(06): 1357-1368 .
    2. 赵旺，陈旭，陈明强，黄星美，邓正华，温为庚，王江勇. 鸢乌贼为蛋白源的方斑东风螺人工配合饲料养殖研究. 广东农业科学. 2023(04): 115-122 .
    3. 田振中，樊丽花，董海隆. 融合随机森林与多变量灰色的道路交通事故预测模型研究. 警察技术. 2023(05): 78-81 .

    Other cited types(4)

Catalog

    Get Citation
    PDF
    XML
    Article views (877) PDF downloads (66) Cited by(7)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return