WANG Jialin, YANG Yingming, YANG Qian, WANG Na, CHEN Songlin. Effects of sex steroid hormones (estradiol and testosterone) on growth traits of female, male and pseduo-male Chinese tongue sole (Cynoglossus semilaevis)[J]. South China Fisheries Science, 2021, 17(4): 27-34. DOI: 10.12131/20210030
Citation: WANG Jialin, YANG Yingming, YANG Qian, WANG Na, CHEN Songlin. Effects of sex steroid hormones (estradiol and testosterone) on growth traits of female, male and pseduo-male Chinese tongue sole (Cynoglossus semilaevis)[J]. South China Fisheries Science, 2021, 17(4): 27-34. DOI: 10.12131/20210030

Effects of sex steroid hormones (estradiol and testosterone) on growth traits of female, male and pseduo-male Chinese tongue sole (Cynoglossus semilaevis)

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  • Received Date: January 14, 2021
  • Revised Date: March 28, 2021
  • Accepted Date: April 06, 2021
  • Available Online: April 15, 2021
  • A variety of fish, including Chinese tongue sole (Cynoglossus semilaevis), have sexual size dimorphism, and this growth dimorphism may be related to the interaction of sex steroid hormones and growth axis-related genes. For the early stage of this study, transcriptomics analysis has found that sex steroid hormones might be involved in the growth regulation of Chinese tongue sole. In order to explore the effect of sex steroid hormones on the growth difference of different sexes of Chinese tongue sole, we injected 16-month-old female, male and pseudo-male fish with estradiol and testosterone hormones, and measured the growth hormone 1 (gh1), insulin-like growth factor 1 (igf1) and suppressor of cytokine signaling 3 (socs3) for quantitative analysis. The results reveal that the two sex steroid hormones had inhibitory effects on the growth performance of individuals of different sexes of Chinese tongue sole. After the treatment, the expressions of gh1 and igf1 in the brain and liver showed an overall downward trend, and the expression of socs3 in the muscle and liver increased significantly, indicating that the growth performance of fish had declined. By exploring the effects of sex steroid hormones on the growth performance of different sexes of Chinese tongue sole, we provide references for the in-depth study of sex steroid hormones in the regulation of fish sex size abnormalities.
  • [1]
    梅洁, 桂建芳. 鱼类性别异形和性别决定的遗传基础及其生物技术操控[J]. 中国科学: 生命科学, 2014, 44(12): 1198-1212.
    [2]
    柳学周, 庄志猛, 马爱军, 等. 半滑舌鳎繁殖生物学及繁育技术研究[J]. 海洋水产研究, 2005, 26(5): 9-16.
    [3]
    陈松林, 李仰真, 张静, 等. 半滑舌鳎快速生长及高雌性家系的筛选[J]. 水产学报, 2013, 37(4): 481-488.
    [4]
    JI X S, LIU H W, CHEN S L, et al. Growth differences and dimorphic expression of growth hormone (GH) in female and male Cynoglossus semilaevis after male sexual maturation[J]. Mar Genom, 2011, 4(1): 9-16. doi: 10.1016/j.margen.2010.11.002
    [5]
    季相山, 陈松林, 马洪雨, 等. 半滑舌鳎养殖群体中自然性逆转伪雄鱼的发现[J]. 水产学报, 2010, 34(2): 322-327.
    [6]
    WEHRENBERG W B, GIUSTINA A. Basic counterpoint: mechanisms and pathways of gonadal steroid modulation of growth hormone secretion[J]. Endocr Rev, 1992, 13(2): 299-308.
    [7]
    代向燕, 张玮, 卓子见, 等. 鱼类生长的神经内分泌调控[J]. 中国科学: 生命科学, 2014, 44(12): 1213-1226.
    [8]
    MA Q, LIU S, ZHUANG Z, et al. Genomic structure, polymorphism and expression analysis of the growth hormone (GH) gene in female and male half-smooth tongue sole (Cynoglossus semilaevis)[J]. Gene, 2012, 493(1): 92-104. doi: 10.1016/j.gene.2011.11.015
    [9]
    NELSON L E, SHERIDAN M A. Regulation of somatostatins and their receptors in fish[J]. Gen Comp Endocrinol, 2005, 142(12): 117-133.
    [10]
    STUDZINSKI A L, ALMEIDA D V, LANES CARLOS F C, et al. SOCS1 and SOCS3 are the main negative modulators of the somatotrophic axis in liver of homozygous GH-transgenic zebrafish (Danio rerio)[J]. Gen Comp Endocrinol, 2009, 161(1): 67-72. doi: 10.1016/j.ygcen.2008.10.008
    [11]
    KHAN M G M, GHOSH A, VARIYA B, et al. Hepatocyte growth control by SOCS1 and SOCS3[J]. Cytokine, 2019, 121: 154733. doi: 10.1016/j.cyto.2019.154733
    [12]
    殷艳蓉, 袁炜, 贺明, 等. 17β-雌二醇通过SOCS3抑制泡沫细胞形成的机制初探[J]. 新疆医科大学学报, 2019, 42(12): 1531-1537. doi: 10.3969/j.issn.1009-5551.2019.12.005
    [13]
    GABRIELSSON B G, CARMIGNAC D F, FLAVELL D M, et al. Steroid regulation of growth hormone (GH) receptor and GH-binding protein messenger ribonucleic acids in the rat[J]. Narnia, 1995, 136(1): 209-217.
    [14]
    DOMENÉ H M, MARÍN G, SZTEIN J, et al. Estradiol inhibits growth hormone receptor gene expression in rabbit liver[J]. Mol Cell Endocrinol, 1994, 103(1/2): 81-87.
    [15]
    BRIAN J H, CHRISTINA M D, STEVEN B C, et al. Non-genomic convergent and divergent signalling of rapid responses to aldosterone and estradiol in mammalian colon[J]. Steroids, 2002, 67(6): 483-491. doi: 10.1016/S0039-128X(01)00169-6
    [16]
    HAZEM A H, WILLIAM J E, ALLEN T, et al. Estrogen and androgen elicit growth hormone release via dissimilar patterns of hypothalamic neuropeptide secretion[J]. Steroids, 2001, 66(2): 71-80. doi: 10.1016/S0039-128X(00)00168-9
    [17]
    MANDIKI S N M, BABIAK I, BOPOPI J M, et al. Effects of sex steroids and their inhibitors on endocrine parameters and gender growth differences in Eurasian perch (Perca fluviatilis) juveniles[J]. Steroids, 2005, 70(2): 85-94. doi: 10.1016/j.steroids.2004.10.009
    [18]
    王凌宇, 齐飘飘, 陈敏, 等. 性类固醇激素对黄颡鱼雌雄生长二态性的影响[J]. 水生生物学报, 2020, 44(2): 379-388. doi: 10.7541/2020.046
    [19]
    马细兰, 张勇, 陈勇智, 等. 性类固醇激素E2、MT对尼罗罗非鱼 (Oreochromis niloticus) 雌、雄生长差异的影响[J]. 海洋与湖沼, 2015, 46(6): 1487-1493.
    [20]
    WANG J, ZHOU J L, YANG Q, et al. Effects of 17 α-methyltestosterone on the transcriptome, gonadal histology and sex steroid hormones in Pseudorasbora parva[J]. Theriogenology, 2020, 155(10): 88-97.
    [21]
    WANG N, WANG R, CHEN S L. Transcriptomics analysis revealing candidate networks and genes for the body size sexual dimorphism of Chinese tongue sole (Cynoglossus semilaevis)[J]. Funct Integr Genomics, 2018, 18(10): 327-339.
    [22]
    刘洋, 陈松林, 高峰涛, 等. 半滑舌鳎性别特异微卫星标记的SCAR转化及其应用[J]. 农业生物技术学报, 2014, 22(6): 787-792. doi: 10.3969/j.issn.1674-7968.2014.06.015
    [23]
    CLEVELAND B M, WEBER G M. Effects of sex steroids on expression of genes regulating growth-related mechanisms in rainbow trout (Oncorhynchus mykiss)[J]. Gen Comp Endocrinol, 2015, 216: 103-115. doi: 10.1016/j.ygcen.2014.11.018
    [24]
    YU Y M, DOMENÉ H M, SZTEIN J, et al. Developmental changes and differential regulation by testosterone and estradiol of growth hormone receptor expression in the rabbit[J]. Eur J Endocrinol, 1996, 135(5): 583-590. doi: 10.1530/eje.0.1350583
    [25]
    BORSKI R J, TSAI W, DEMOTT F R, et al. Regulation of somatic growth and the somatotropic axis by gonadal steroids: primary effect on insulin-like growth factor I gene expression and secretion[J]. Endocrinology, 1996, 137(8): 3253-3259. doi: 10.1210/endo.137.8.8754747
    [26]
    HOLLOWAY A C, LEATHERLAND J F. Effect of gonadal steroid hormones on plasma growth hormone concentrations in sexually immature rainbow trout, Oncorhynchus mykiss[J]. Gen Comp Endocrinol, 1997, 105(2): 246-254. doi: 10.1006/gcen.1996.6826
    [27]
    REINECKE M. Insulin-like growth factors and fish reproduction[J]. Biol Reprod, 2010, 82(4): 656-661. doi: 10.1095/biolreprod.109.080093
    [28]
    PÉREZ S J, SIMÓ M P, NAYA C F, et al. Somatotropic axis regulation unravels the differential effects of nutritional and environmental factors in growth performance of marine farmed fishes[J]. Front Endocrinol (Lausanne), 2008, 9(10): 682-689.
    [29]
    CLEVELAND B M, WEBER G M. Effects of steroid treatment on growth, nutrient partitioning, and expression of genes related to growth and nutrient metabolism in adult triploid rainbow trout (Oncorhynchus mykiss)[J]. Domest Anim Endocrinol, 2016, 56(10): 1-12.
    [30]
    KANAKARAJU K, RUAIRI C R, KIERA M, et al. Estrogen-mediated gut microbiome alterations influence sexual dimorphism in metabolic syndrome in mice[J]. Microbiome, 2018, 6(1): 20-25. doi: 10.1186/s40168-018-0404-9
    [31]
    MEINHARDT U J, HO K Y. Modulation of growth hormone action by sex steroids[J]. Clin Endocrinol (Oxf), 2006, 65(4): 413-422. doi: 10.1111/j.1365-2265.2006.02676.x
    [32]
    MARTYNIUK C J, BISSEGGER S, LANGLOIS V S. Current perspectives on the androgen 5 alpha-dihydrotestosterone (DHT) and 5 alpha-reductases in teleost fishes and amphibians[J]. Gen Comp Endocrinol, 2013, 194(12): 264-274.
    [33]
    PERI A, DANZA G, BENVENUTI S, et al. New insights on the neuroprotective role of sterols and sex steroids: the seladin-1/DHCR24 paradigm[J]. Front Neuroendocrin, 2009, 30(2): 119-129. doi: 10.1016/j.yfrne.2009.03.006
    [34]
    LUCIANI P, DELEDDA C, ROSATI F, et al. Seladin-1 is a fundamental mediator of the neuroprotective effects of estrogen in human neuroblast long-term cell cultures[J]. Endocrinology, 2008, 149(9): 4256-4266. doi: 10.1210/en.2007-1795
    [35]
    NELSON P S, CLEGG N, ARNOLD H, et al. The program of androgen-responsive genes in neoplastic prostate epithelium[J]. Proc Nat Acad Sci USA, 2002, 99(18): 11890-11895. doi: 10.1073/pnas.182376299
    [36]
    MENDEZ P, WANDOSELL F, GARCIA S, et al. Cross-talk between estrogen receptors and insulin-like growth factor-I receptor in the brain: cellular and molecular mechanisms[J]. Front Neuroendocrin, 2006, 27(4): 391-403. doi: 10.1016/j.yfrne.2006.09.001
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