Effects of low salinity pressure on biological tissue and immunity enzymes activities of <i>Sipunculus nudus</i>

LI Junwei; HU Ruiping; CHEN Suwen; GUO Yongjian; ZHU Changbo; LI Ting; XIE Xiaoyong; SU Jiaqi

doi:10.12131/20210022

In order to understand the response of the biological and physiological indicators of Sipunculus nudus to low salinity, we evaluated the effects of low salinity pressure on the survival rate, osmotic pressure, biological tissue and immunity enzymes activities of S. nudus which were cultured for 96 h in water with abrupt salinity drop of 0 (S30), 5 (S25), 10 (S20), 15 (S15), 20 (S10) and 25 (S5). The results show that: 1) S. nudus could tolerate the salinity drop of 10, but would die when the salinity dropped over 15. 2) The coelomic fluid of S. nudus increased with the decrease of water salinity significantly, and there was a significant positive correlation between the body mass and coelomic fluid volumn (R²>0.96). 3) The osmotic pressure and body protein contents decreased with the decrease of water salinity (P<0.05). 4) In the low salinity treatments, the connective tissue of longitudinal and circumferential muscle fibers of the body wall were thin, and the staining of columnar cells was lighter. For the tentacle, its adhesion ability was weaker as the epithelial cells became larger. 5) SOD and CAT activities in the groups of S30 and S25 were relatively stable, while the body SOD and coelomic fluid CAT in the groups of S10 and S15 first decreased and then increased; however, the CAT of coelomic fluid in the groups of S10 and S15 first increased and then decreased. SOD and CAT activities in the coelomic fluid had strong varation. In summary, low salinity pressure can affect the survival rate, osmotic pressure, biological tissue and immunity enzymes activities of S. nudus significantly.

[1]	LIU Y, WANG W N, WANG A L, et al. Effects of dietary vitamin E supplementation on antioxidant enzyme activities in Litopenaeus vannamei (Boone, 1931) exposed to acute salinity changes[J]. Aquaculture, 2007, 265(1): 351-358.
[2]	ANNI I S A, BIANCHINI A, BARCAROLLI I F, et al. Salinity influence on growth, osmoregulation and energy turnover in juvenile pompano Trachinotus marginatus Cuvier 1832[J]. Aquaculture, 2016, 455: 63-72. doi: 10.1016/j.aquaculture.2016.01.010
[3]	ADRIANOV A V, MAIOROVA A S. Reproduction and development of common species of peanut worms (Sipuncula) from the Sea of Japan[J]. Rus J Mar Biol, 2010, 36(1): 1-15. doi: 10.1134/S1063074010010013
[4]	周红, 李凤鲁, 王玮. 星虫动物门中国动物志无脊椎动物第46卷[M]. 北京: 科学出版社, 2007: 15-113.
[5]	李俊伟, 朱长波, 颉晓勇, 等. 方格星虫的繁育、养殖及研究开发进展[J]. 南方水产科学, 2014, 10 (5): 94-98. doi: 10.3969/j.issn.2095-0780.2014.05.014
[6]	曾志南, 刘伟斌, 林向阳, 等. 光裸方格星虫初期海球幼体对温度和盐度的耐受试验[J]. 福建水产, 2010, 32(1): 14-18. doi: 10.3969/j.issn.1006-5601.2010.01.007
[7]	代悦, 王庆恒, 陈桂侬, 等. 光裸星虫对盐度和温度的耐受性研究[J]. 水产科学, 2009, 28 (10): 563-566. doi: 10.3969/j.issn.1003-1111.2009.10.003
[8]	杨红生, 周毅, 张涛. 刺参生物学: 理论与实践[M]. 北京: 科学出版社, 2014: 30-41.
[9]	BURG M B. Macromolecular crowding as a cell volume sensor[J]. Cell Physiol Biochem, 2000, 10 (5/6): 251-256.
[10]	KULTZ D, CHAKRAVARTY D. Hyperosmolality in the form of elevated NaCl but not urea causes DNA damage in murine kidney cells[J]. Proc Nat Acad Sci USA, 2001, 98(4): 1999-2004. doi: 10.1073/pnas.98.4.1999
[11]	屈亮, 庄平, 章龙珍, 等. 盐度对俄罗斯鲟幼鱼血清渗透压、离子含量及鳃丝 Na⁺/K⁺-ATP 酶活力的影响[J]. 中国水产科学, 2010, 17(2): 243-251.
[12]	包杰, 姜宏波, 董双林, 等. 红刺参和青刺参耗氧率与排氨率的比较研究[J]. 水产学报, 2013, 37 (11): 1589-1696.
[13]	庚辰帆, 田燚, 张宇鹏, 等. 低盐急性胁迫对仿刺参相关生理指标的影响[J]. 中国水产科学, 2015, 22 (4): 666-674.
[14]	MENG X L, DONG Y W, DONG S L, et al. Mortality of the sea cucumber, Apostichopus japonicus Selenka, exposed to acute salinity decrease and related physiological responses: osmo regulation and heat shock protein expression[J]. Aquaculture, 2011, 316 (1/2/3/4): 88-92.
[15]	邢坤, 杨红生. 刺参血淋巴细胞形态和表面结构观察[J]. 海洋科学, 2008, 32 (6): 52-54.
[16]	黄洪艳. 裸体方格星虫(Sipunculus nudus)体腔液细胞分类及超微结构[D]. 湛江: 广东海洋大学, 2006: 3-36.
[17]	LIU Y X, ZHOU D Y, LIU Z Q, et al. Structural and biochemical changes in dermis of sea cucumber (Stichopus japonicus) during autolysis in response to cutting the body wall[J]. Food Chem, 2018, 240: 1254-1261. doi: 10.1016/j.foodchem.2017.08.071
[18]	李二超, 陈立桥, 曾嶒, 等. 不同盐度下饵料蛋白质含量对凡纳滨对虾生长、体成分和肝胰组织结构的影响[J]. 水产学报, 32(3): 425-433.
[19]	韩晓琳, 高保全, 王好锋, 等. 低盐胁迫对三疣梭子蟹鳃和肝胰腺显微结构及家系存活的影响[J]. 渔业科学进展, 2014, 35(1): 104-110. doi: 10.3969/j.issn.1000-7075.2014.01.015
[20]	ROSS S W, DALTON D A, KRAMER S, et al. Physiological (antioxidant) responses of estuarine fishes to variability in dissolved oxygen[J]. Comp Biochem Physiol C, 2001, 130(3): 289-303.
[21]	徐永健, 孙彬. 盐度胁迫对大海马幼体生长、组分及酶活力的影响[J]. 海洋与湖沼, 2012, 43 (6): 1279-1285. doi: 10.11693/hyhz201206036036
[22]	张晨捷, 张艳亮, 高权新, 等. 低盐胁迫对黄姑鱼幼鱼肝脏抗氧化功能的影响[J]. 南方水产科学, 2015, 11(4): 59-64. doi: 10.3969/j.issn.2095-0780.2015.04.009
[23]	ZHANG M, LI L, LIU Y, et al. Effects of a sudden drop in salinity on Scapharca subcrenata antioxidant defenses and metabolism determined using LCMS non-targeted metabolomics[J]. Sci Rep, 2020, 10(1): 7324. doi: 10.1038/s41598-020-63293-0
[24]	胡静, 叶乐, 吴开畅, 等. 急性盐度胁迫对克氏双锯鱼幼鱼血清皮质醇浓度和Na⁺-K⁺-ATP酶活性的影响[J]. 南方水产科学, 2016, 12 (2): 116-120. doi: 10.3969/j.issn.2095-0780.2016.02.017
[25]	叶建生, 王兴强, 马甡, 等. 盐度突变对凡纳滨对虾非特异性免疫因子的影响[J]. 海洋水产研究, 2008, 29 (1): 38-43.
[26]	张玉玉, 王春琳, 李来国. 长蛸的盐度耐受性及盐度胁迫对其血细胞和体内酶活力的影响[J]. 台湾海峡, 2010, 29 (4): 452-459.
[27]	郑慧, 李彬, 荣小军, 等. 盐度和溶解氧对刺参非特异性免疫酶活性的影响[J]. 渔业科学进展, 2014, 35(1): 118-123. doi: 10.3969/j.issn.1000-7075.2014.01.017
[28]	季延滨, 于雯雯, 孙金辉, 等. 盐度骤降对南美白对虾仔虾抗氧化机能的影响[J]. 天津农学院学报, 2008, 15(4): 19-23. doi: 10.3969/j.issn.1008-5394.2008.01.007
[29]	沈晔, 王兴强, 曹梅, 等. 脊尾白虾对低盐胁迫响应的转录组学分析[J]. 南方水产科学, 2020, 16 (5): 19-32. doi: 10.12131/20190267
[30]	LI Q, LI W Q, LIANG X F, et al. Gill transcriptome analysis reveals the molecular response to the acute low-salinity stress in Cyclina sinensis[J]. Aquacult Rep, 2021, 19: 100564.
[31]	何鹏, 江世贵, 李运东, 等. 斑节对虾GLUT1基因cDNA的克隆与表达分析[J]. 南方水产科学, 2019, 15(2): 72-82. doi: 10.12131/20180264

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Effects of low salinity pressure on biological tissue and immunity enzymes activities of Sipunculus nudus

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