Volume 17 Issue 5
Sep.  2021
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Yuting WANG, Rongxiang ZHOU, Jihong LI, Yao ZHANG, Tingting ZHOU, Wencai CHEN, Yun PENG, Manli TANG, Guizhen MA, Jianhe XU. Isolation and identification of vibrio resistant photosynthetic bacteria and degradation of nitrite nitrogen and ammonia nitrogen[J]. South China Fisheries Science, 2021, 17(5): 26-33. DOI: 10.12131/20210016
Citation: Yuting WANG, Rongxiang ZHOU, Jihong LI, Yao ZHANG, Tingting ZHOU, Wencai CHEN, Yun PENG, Manli TANG, Guizhen MA, Jianhe XU. Isolation and identification of vibrio resistant photosynthetic bacteria and degradation of nitrite nitrogen and ammonia nitrogen[J]. South China Fisheries Science, 2021, 17(5): 26-33. DOI: 10.12131/20210016
shu

Isolation and identification of vibrio resistant photosynthetic bacteria and degradation of nitrite nitrogen and ammonia nitrogen

  • 1.

    School of Environmental and Chemical Engineering, Jiangsu Ocean University, Lianyungang 252000, China

  • 2.

    School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang 222000, China

  • 3.

    Yongqing County Agricultural and Rural Bureau of Plantation Technology Extension Station, Langfang 065000, China

  • 4.

    School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222000, China

More Information
  • Received Date: January 11, 2021
  • Revised Date: March 08, 2021
  • Accepted Date: April 01, 2021
  • Available Online: April 18, 2021
    Graphical Abstract
    • Abstract
  • In this study, photosynthetic bacteria (PSB) were isolated and purified from marine environmental samples from different areas by double-layer plate coating method and scribing method. Vibrio parahaemolyticus, V. vulnificus and V. anguillarum were used as control. The inhibition of marine photosynthetic bacteria was determined by Oxford cup method, and the degradation of nitrite nitrogen (NO2 −-N) and ammonia nitrogen (NH4 +-N) by different strains was determined by naphthalene ethylenediamine hydrochloride spectrophotometry and indophenol blue spectrophotometry. The results show that three strains of photosynthetic bacteria were isolated from 30 sea water and mud samples, and the P-3 strain isolated from the seawater samples of Cheniushan Island in Lianyungang had strong inhibitory effect on three kinds of Vibrio, especially for V. anguillarum, with the inhibition zone diameter of 5.3 mm. The results show that all the three photosynthetic bacteria have certain ability to degrade NO2 −-N and NH4 +-N, and P-3 strain had the strongest ability. The degradation rates of P-3 strain were 89.68% and 94.98% respectively, when being cultured in the medium containing 50 mg·L−1 NH4 +-N and NO2 −-N for 4 d. P-3 strain was identified as Rhodopseudomonas palustris by morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis.
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