Volume 17 Issue 5
Sep.  2021
Turn off MathJax
Article Contents
Abstract
References
Related Articles
Jiaqi SU, Huaping ZHU, Changbo ZHU, Bo ZHANG, Ting LI, Suwen CHEN. Effects of salinity and Na+/K+ ratio on survival and histological structure of Litopenaeus vannamei[J]. South China Fisheries Science, 2021, 17(5): 45-53. DOI: 10.12131/20210011
Citation: Jiaqi SU, Huaping ZHU, Changbo ZHU, Bo ZHANG, Ting LI, Suwen CHEN. Effects of salinity and Na+/K+ ratio on survival and histological structure of Litopenaeus vannamei[J]. South China Fisheries Science, 2021, 17(5): 45-53. DOI: 10.12131/20210011
shu

Effects of salinity and Na+/K+ ratio on survival and histological structure of Litopenaeus vannamei

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Development & Utilization, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecological Environment, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

More Information
  • Received Date: January 07, 2021
  • Revised Date: April 01, 2021
  • Accepted Date: April 27, 2021
  • Available Online: July 14, 2021
    Graphical Abstract
    • Abstract
  • By using method of experimental ecology, we studied the effects of salinity (2, 4, 8, 12, 16) and Na+/K+ (27, 50, 75, 100, 125 and 150) ratio on 72 h survival rate, hepatopancreas and muscle structure of Litopenaeus vannamei juveniles with body mass of (1.35 ± 0.37) g under experimental condition. The results show that survival rate of L. vannamei decreased with increasing salinity when the Na+/K+ ratio was above 75. In addition, when the water salinity was over 8, the survival rate increased with decreasing Na+/K+ ratio. The survival rate of L. vannamei was above 66.67% when the Na+/K+ ratio was 27−75. When the Na+/K+ ratio was 100, the half lethal time (LT50) of L. vannamei was 69.78 and 60.15 h at salinity of 12 and 16, respectively. When the Na+/K+ ratio was 125, the LT50 at salinity of 8, 12 and 16 was 76.23, 62.61 and 49.10 h, respectively. When the Na+/K+ ratio was 150, the LT50 at salinity of 4, 8, 12 and 16 was 87.24, 68.65, 59.4 and 39.95 h, respectively. Based on the two-factor variance analysis, the 72 h survival rate of L. vannamei was significantly influenced by salinity, Na+/K+ ratio and their interactions (P<0.001). Histopathology observation reveals that high Na+/K+ ratio can cause abnormal histological change in shrimp muscle and hepatopancreas, such as cell vacuolation or autolysis, larger intercellular space, fuzzy and disordered boundary between tissues. In general, potassium deficiency can aggravate the tissue injury of L. vannamei with higher salinity.
    • FullText(HTML)
    • References (37)
  • [1]
    刘永新, 方辉, 来琦芳, 等. 我国盐碱水渔业现状与发展对策[J]. 中国工程科学, 2016, 18(3): 74-78. doi: 10.3969/j.issn.1009-1742.2016.03.013
    [2]
    梁利群, 任波, 常玉梅, 等. 中国内陆咸(盐碱)水资源及渔业综合开发利用[J]. 中国渔业经济, 2013, 31(4): 138-145. doi: 10.3969/j.issn.1009-590X.2013.04.021
    [3]
    王芸, 李正, 段亚飞, 等. 红景天提取物对凡纳滨对虾抗氧化系统及抗低盐度胁迫的影响[J]. 南方水产科学, 2018, 14(1): 9-19. doi: 10.3969/j.issn.20950780.2018.01.002
    [4]
    农业农村部渔业渔政管理局. 2020中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2020: 21-30.
    [5]
    曾凡勇, 罗坤, 栾生, 等. 凡纳滨对虾在氯化物型盐碱水养殖环境下不同家系间生长、存活性能分析[J]. 中国水产科学, 2018, 25(2): 308-315.
    [6]
    朱长波, 董双林. 对虾内陆养殖的现状、存在的问题及其展望[J]. 南方水产, 2005, 1(5): 63-69.
    [7]
    刘泓宇, 张新节, 谭北平, 等. 饲料钾水平对低盐井水养殖凡纳滨对虾生长及生理特性的影响[J]. 中国水产科学, 2014, 21(2): 320-329.
    [8]
    JESCOVITCH L N, PINE H J, ROY L A, et al. Growth of Pacific white shrimp, Litopenaeus vannamei, cultured on inland low salinity pond soils[J]. N Am J Aquacult, 2018, 80(3): 273-277. doi: 10.1002/naaq.10031
    [9]
    WUDTISIN I, BOYD C E. Possible potassium and magnesium limitations for shrimp survival and production in low-salinity, pond waters in Thailand[J]. J World Aquacult Soc, 2011, 42(6): 766-777. doi: 10.1111/j.1749-7345.2011.00530.x
    [10]
    BOYD C A, BOYD C E, ROUSE D B. Potassium budget for inland, saline water shrimp ponds in Alabama[J]. Aquacult Eng, 2007, 36(1): 45-50. doi: 10.1016/j.aquaeng.2006.06.002
    [11]
    LI E C, WANG X D, CHEN K, et al. Physiological change and nutritional requirement of Pacific white shrimp Litopenaeus vannamei at low salinity[J]. Rev Aquacult, 2017, 9(1): 57-75. doi: 10.1111/raq.12104
    [12]
    ZHU C B, DONG S L, WANG F, et al. Effects of Na/K ratio in seawater on growth and energy budget of juvenile Litopenaeus vannamei[J]. Aquaculture, 2004, 234(1/2/3/4): 485-496.
    [13]
    LIU H Y, TAN B P, YANG J F, et al. Effects of aqueous Na/K and dietary K on growth and physiological characters of the Pacific white shrimp, Litopenaeus vannamei, reared in low-salt well water[J]. Aquacult Res, 2016, 47(2): 540-553. doi: 10.1111/are.12513
    [14]
    VALENZUELA-MADRIGAL I E, VALENZUELA-QUIÑÓNEZ W, ESPARZA-LEAL H M, et al. Effects of ionic composition on growth and survival of white shrimp Litopenaeus vannamei culture at low-salinity well water[J]. Rev Biol Mar Oceanog, 2017, 52(1): 103-112. doi: 10.4067/S0718-19572017000100008
    [15]
    PATHAK M S, REDDY A K, KULKAMI M V, et al. Histological alterations in the hepatopancreas and growth performance of Pacific white shrimp (Litopenaeus vannamei, Boone 1931) reared in potassium fortified inland saline ground water[J]. Int J Curr Microbiol App Sci, 2018, 7(4): 3531-3542. doi: 10.20546/ijcmas.2018.704.398
    [16]
    刘存歧, 王军霞, 张亚娟, 等. 盐碱地渗水盐度与钠钾比对凡纳滨对虾生长的影响[J]. 应用生态学报, 2008, 19(6): 1337-1342.
    [17]
    ZHU C B, DONG S L, WANG F. The interaction of salinity and Na/K ratio in seawater on growth, nutrient retention and food conversion of juvenile Litopenaeus vannamei[J]. J Shellfish Res, 2007, 25(1): 107-112.
    [18]
    魏杰, 曹希全, 任永丽, 等. 宽口裂腹鱼消化系统解剖和组织学观察[J]. 南方水产科学, 2020, 16(1): 120-126. doi: 10.12131/20180262
    [19]
    HONKANEN J O, REES C B, KUKKONEN J V K, et al. Temperature determines the rate at which retene affects trout embryos, not the concentration that is toxic[J]. Aquat Toxicol, 2020, 222: 105471. doi: 10.1016/j.aquatox.2020.105471
    [20]
    RAMIREZA J L, MUTURIA E J, BARLETTA A B F, et al. The Aedes aegypti IMD pathway is a critical component of the mosquito antifungal immune response[J]. Dev Comp Immunol, 2019, 95: 1-9. doi: 10.1016/j.dci.2018.12.010
    [21]
    王倩, 刘利平, 陈文银, 等. 钠、钾离子浓度及比例对鳗鲡早期发育的影响[J]. 上海海洋大学学报, 2015, 24(6): 834-840. doi: 10.12024/jsou.20150301370
    [22]
    PILLARD D A, DUFRESNE D L, CAUDLE D D, et al. Predicting the toxicity of major ions in seawater to mysid shrimp (Mysidopsis bahia), sheepshead minnow (Cyprinodon variegatus), and inland silverside minnow (Menidia beryllina)[J]. Environ Toxicol Chem, 2000, 19(1): 183-191. doi: 10.1002/etc.5620190122
    [23]
    FORSBERG, J A, DORSETT P W, NEILL W H. Survival and growth of red drum Sciaenops ocellatus in saline groundwaters of West Texas[J]. J World Aquacult Soc, 1996, 27(4): 462-474. doi: 10.1111/j.1749-7345.1996.tb00631.x
    [24]
    PRANGNELL D I, FOTEDAR R. Effect of sudden salinity change on Penaeus latisulcatus Kishinouye osmoregulation, ionoregulation and condition in inland saline water and potassium-fortified inland saline water[J]. Comp Biochem Phys A, 2006, 145(4): 449-457. doi: 10.1016/j.cbpa.2006.08.029
    [25]
    ROY L A, DAVOS D A, SAOID I P, et al. Effects of varying levels of aqueous potassium and magnesium on survival, growth, and respiration of the Pacific white shrimp, Litopenaeus vannamei, reared in low salinity waters[J]. Aquaculture, 2007, 262(2/3/4): 461-469.
    [26]
    PEREZ-VELAZQUEZ M, DAVIS D A, ROY L A, et al. Effects of water temperature and Na+: K+ ratio on physiological and production parameters of Litopenaeus vannamei reared in low salinity water[J]. Aquaculture, 2012, 342/343(1): 13-17.
    [27]
    刘慧玲, 陈荣, 王成桂, 等. 不同盐度条件下氨氮和亚硝酸盐对墨吉明对虾的急性毒性试验[J]. 江苏农业科学, 2019, 47(1): 155-158.
    [28]
    LIN Y C, CHEN J C. Acute toxicity of ammonia on Litopenaeus vannamei Boone juveniles at different salinity levels[J]. J Exp Mar Biol Ecol, 2001, 259(1): 109-119. doi: 10.1016/S0022-0981(01)00227-1
    [29]
    LIN Y C, CHEN J C. Acute toxicity of nitrite on Litopenaeus vannamei (Boone) juveniles at different salinity levels[J]. Aquaculture, 2003, 224(1/2/3/4): 193-201.
    [30]
    PARTRIDGE G J, LYMBERY A J. The effect of salinity on the requirement for potassium by barramundi (Lates calcarifer) in saline groundwater[J]. Aquaculture, 2008, 278(1/2/3/4): 164-170.
    [31]
    TANTULO U, FOTEDAR R. Osmo and ionic regulation of black tiger prawn (Penaeus monodon Fabricius 1798) juveniles exposed to K+ deficient inland saline water at different salinities[J]. Comp Biochem Phys A, 2007, 146(2): 208-214. doi: 10.1016/j.cbpa.2006.10.020
    [32]
    赵光远, 王荃. 高钾血症和低钾血症危象[J]. 中国小儿急救医学, 2020, 27(8): 572-576. doi: 10.3760/cma.j.issn.1673-4912.2020.08.003
    [33]
    KNOCHEL J P, SCHLEIN E M. On the mechanism of rhabdomyolysis in potassium depletion[J]. J Clin Invest, 1972, 51(7): 1750-1758. doi: 10.1172/JCI106976
    [34]
    陆敏, 徐荣, 王迎春. 高频超声心动图观察低血钾对兔心脏结构及功能的影响[J]. 实验动物与比较医学, 2010, 30(6): 424-427. doi: 10.3969/j.issn.1674-5817.2010.06.007
    [35]
    杨明, 孙盛明, 傅洪拓, 等. 低氧和复氧对日本沼虾抗氧化酶活力及组织结构的影响[J]. 中国水产科学, 2019, 26(3): 493-503.
    [36]
    陶易凡, 强俊, 王辉, 等. 低pH胁迫对克氏原螯虾鳃和肝胰腺酶活力及组织结构的影响[J]. 中国水产科学, 2016, 23(6): 1279-1289.
    [37]
    梁忠秀, 李健, 李吉涛, 等. 塔玛亚历山大藻毒素及其对中国对虾的急性毒性[J]. 中国水产科学, 2014, 21(6): 1260-1267.
    • Related Articles

  • Related Articles

    [1]LI Yu, HUANG Jiansheng, CHEN Youming, WEN Zhenwei, OU Guanghai, HUANG Jianpeng, JIANG Xintao, XIE Ruitao, MA Qian, CHEN Gang. Effect of low temperature stress on antioxidant stress, apoptosis and histological structure of gills in cobia (Rachycentron canadum)[J]. South China Fisheries Science, 2023, 19(3): 68-77. DOI: 10.12131/20220227
    [2]CHEN Jie, HE Yang, DAI Xuping, WANG Jun, QING Chuanjie, LI Rui. Histological observation and innate immune barrier study of head kidney of Pelteobagrus vachelli[J]. South China Fisheries Science, 2021, 17(1): 82-90. DOI: 10.12131/20200119
    [3]WANG Jian, ZENG Benhe, XU Zhaoli, ZHANG Bianbian, LIU Haiping, WANG Wanliang, WANG Jinlin, ZHOU Jianshe, HUANG Liping. Effect of dietary protein level on digestive enzyme activity and histological structure in intestine and liver of juvenile Schizopygopsis younghusbandi[J]. South China Fisheries Science, 2019, 15(6): 112-119. DOI: 10.12131/20190107
    [4]MA Dingchang, YE Liuhe, XU Aiyu, PAN Gan, LONG Cheng. A histological study of Tylorrhynchus heterochaetus[J]. South China Fisheries Science, 2014, 10(4): 58-63. DOI: 10.3969/j.issn.2095-0780.2014.04.010
    [5]LIU Zaijun, CEN Jianwei, LI Laihao, YANG Xianqing, HAO Shuxian, WEI Ya, ZHOU Wanjun. Thoughts and prospect of comprehensive utilization of tilapia blood[J]. South China Fisheries Science, 2012, 8(2): 76-80. DOI: 10.3969/j.issn.2095-0780.2012.02.012
    [6]WANG Jingxiang, LI Jia′er, OU Youjun, WANG Yongcui. Ultrastructure of peripheral blood cells of highfin grouper Cromileptes altivelis[J]. South China Fisheries Science, 2011, 7(5): 50-54. DOI: 10.3969/j.issn.2095-0780.2011.05.008
    [7]HU Lingling, LI Jia'er, OU Youjun, YU Na, WANG Gang. Study on microstructure of head-kidney and spleen of Oplegnathus fasciatus[J]. South China Fisheries Science, 2010, 6(3): 41-45. DOI: 10.3969/j.issn.1673-2227.2010.03.008
    [8]LAO Haihua, YE Xing, ZOU Weimin, BAI Junjie, TAN Aiping. Detection of infectious spleen and kidney necrosis virus (ISKNV) of mandarin fish (Siniperca chuatsi) by nested PCR[J]. South China Fisheries Science, 2009, 5(4): 69-72. DOI: 10.3969/j.issn.1673-2227.2009.04.013
    [9]SU Youlu, XU Liwen, FENG Juan, GUO Zhixun, WANG Jiangyong. Study on the morphology of peripheral blood cells of juvenile cobia Rachycentron canadum[J]. South China Fisheries Science, 2007, 3(1): 48-53.
    [10]HUANG Jian-hua, MA Zhi-ming, ZHOU Fa-lin, YE Le, JIANG Shi-gui. Study on anatomy structure and histology of the ovary of wild Penaeus monodon(Fabricius) from the north of South China Sea[J]. South China Fisheries Science, 2005, 1(3): 49-53.

  • Cited by

    Periodical cited type(2)

    1. 安余梁，祁峰，母锐敏，马桂霞. 微藻除磷机理及其工艺模式的研究进展. 工业水处理. 2025(02): 10-17 .
    2. 马林，李春艳，李明泽，罗鑫，丁子元，孟睦涵，徐林通，姜巨峰. 天津市碳汇渔业潜力研究及未来展望. 海洋湖沼通报(中英文). 2024(04): 81-88 .

    Other cited types(2)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(4)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return