Volume 17 Issue 5
Sep.  2021
Turn off MathJax
Article Contents
Abstract
References
Related Articles
Qingzhao LIAO, Fuyan CHEN, Ya QIN, Chanchan HUANG, Luodong HUANG, Peihong SHEN. Effects of feeding Candida ethanolica GXU01 on growth, immunity and intestinal flora of tilapia[J]. South China Fisheries Science, 2021, 17(5): 10-17. DOI: 10.12131/20200258
Citation: Qingzhao LIAO, Fuyan CHEN, Ya QIN, Chanchan HUANG, Luodong HUANG, Peihong SHEN. Effects of feeding Candida ethanolica GXU01 on growth, immunity and intestinal flora of tilapia[J]. South China Fisheries Science, 2021, 17(5): 10-17. DOI: 10.12131/20200258
shu

Effects of feeding Candida ethanolica GXU01 on growth, immunity and intestinal flora of tilapia

  • 1.

    Guangxi University, Nanning 530005, China

  • 2.

    Guangxi Academy of Fishery Sciences, Nanning 530021, China

More Information
  • Received Date: December 16, 2020
  • Revised Date: March 15, 2021
  • Accepted Date: March 22, 2021
  • Available Online: April 11, 2021
    Graphical Abstract
    • Abstract
  • To explore the probiotic potential of Candida ethanolica GXU01 in tilapia culture and to find ecological and friendly feeding bacteria for tilapia sustainable cultivation, we used C. ethanolica GXU01 as feed-additive to feed tilapia. Then we determined the growth performance, intestinal digestive enzymes, serum non-specific immune indexes and intestinal microbial community structure, and challenged the tilapia with Streptococcus agalactiae, so as to comprehensively evaluate the effects of C. ethanolica GXU01 on the growth and immunity of tilapia. The results show that the growth performance, digestive enzyme activity, serum lysozyme activity and complement C3 content of tilapia could be significantly improved by feeding C. ethanolica GXU01 (P<0.05). The abundance of Fusobacteria, Cetobacterium and Akkermansia in the intestinal tract of tilapia increased significantly and those of Cyanobacteria decreased significantly after feed of C. ethanolica GXU01. In the challenge test, the survival rate of tilapia fed with diet containing C. ethanolica GXU01 increased by 26.66%. The study shows that C. ethanolica GXU01 promotes the intestinal digestive ability and immune resistance of tilapia.
    • FullText(HTML)
    • References (37)
  • [1]
    WANG M, LU M. Tilapia polyculture: a global review[J]. Aquacult Res, 2016, 47(8): 2 363-2 374. doi: 10.1111/are.12708
    [2]
    ZHU J, GAN X, AO Q, et al. Basal polarization of the immune responses to Streptococcus agalactiae susceptible and resistant tilapia (Oreochromis niloticus)[J]. Fish Shellfish Immunol, 2018, 75: 336-345. doi: 10.1016/j.fsi.2018.01.022
    [3]
    LIU L, LI Y W, HE R Z, et al. Outbreak of Streptococcus agalactiae infection in barcoo grunter, Scortum barcoo (McCulloch & Waite), in an intensive fish farm in China[J]. J Fish Dis, 2014, 37(12): 1 067-1 072. doi: 10.1111/jfd.12187
    [4]
    DAWOOD M A O, KOSHIO S, AAGELES E M. Beneficial roles of feed additives as immunostimulants in aquaculture: a review[J]. Reuv Aquacult, 2018, 10(4): 950-974. doi: 10.1111/raq.12209
    [5]
    REYES B M, TOVAR R D, ASCENCIO V F, et al. Effects of dietary supplementation with probiotic live yeast Debaryomyces hansenii on the immune and antioxidant systems of leopard grouper Mycteroperca rosacea infected with Aeromonas hydrophila[J]. Aquacult Res, 2011, 42(11): 1676-1686. doi: 10.1111/j.1365-2109.2010.02762.x
    [6]
    ABDEL T M. Interactive effects of dietary protein and live bakery yeast, Saccharomyces cerevisiae on growth performance of Nile tilapia, Oreochromis niloticus (L.) fry and their challenge against Aeromonas hydrophila infection[J]. Aquacult Int, 2012, 20(2): 317-331. doi: 10.1007/s10499-011-9462-8
    [7]
    SONMEZ A Y. Evaluating two different additive levels of fully autolyzed yeast, Saccharomyces cerevisiae, on rainbow trout (Oncorhynchus mykiss) growth performance, liver histology and fatty acid composition[J]. Turk J Fish Aquat Sc, 2017, 17(2): 379-385.
    [8]
    AYIKU S, SHEN J, TAN B, et al. Effects of dietary yeast culture on shrimp growth, immune response, intestinal health and disease resistance against Vibrio harveyi[J]. Fish Shellfish Immunol, 2020, 102: 286-295. doi: 10.1016/j.fsi.2020.04.036
    [9]
    MOHSEN A T, MAMDOUH A A M, MAALY A M. Use of live baker's yeast, Saccharomyces cerevisiae, in practical diet to enhance the growth performance of galilee tilapia, Sarotherodon galilaeus (L.), and its resistance to environmental copper toxicity[J]. J World Aquacult Soc, 2010, 41: 1-5.
    [10]
    RUGGIRELLO M, NUCERA D, CANNONI M, et al. Antifungal activity of yeasts and lactic acid bacteria isolated from cocoa bean fermentations[J]. Food Res Int, 2019, 115: 519-525. doi: 10.1016/j.foodres.2018.10.002
    [11]
    XING X, WANG Y, HUO N, et al. Candida ethanolica strain Y18 enhances aroma of Shanxi aged-vinegar[J]. Food Sci Technol Res, 2018, 24(6): 1069-1081. doi: 10.3136/fstr.24.1069
    [12]
    COULIBALY W H, BOUATENIN K M J, KOUANE A K, et al. Use of non-Saccharomyces yeast strains as starter cultures to enhance fermented mango juice production[J]. Sci Afr, 2020, 7: 220-226.
    [13]
    FERNANDES T, CARVALLHO B F, MANTOVANI H C, et al. Identification and characterization of yeasts from bovine rumen for potential use as probiotics[J]. J Appl Microbiol, 2019, 127(3): 845-855. doi: 10.1111/jam.14350
    [14]
    INOUE S, SUZUKI U K, KOMORI Y, et al. Fermentation of non-sterilized fish biomass with a mixed culture of film-forming yeasts and lactobacilli and its effect on innate and adaptive immunity in mice[J]. J Biosci Bioeng, 2013, 116(6): 682-687. doi: 10.1016/j.jbiosc.2013.05.022
    [15]
    SAPUTRA F, SHIU Y, CHEN Y, et al. Dietary supplementation with xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus)[J]. Fish Shellfish Immunol, 2016, 58: 397-405. doi: 10.1016/j.fsi.2016.09.046
    [16]
    ULLAH A, ZUBERI A, AHMAD M, et al. Dietary administration of the commercially available probiotics enhanced the survival, growth, and innate immune responses in Mori (Cirrhinus mrigala) in a natural earthen polyculture system[J]. Fish Shellfish Immunol, 2018, 72: 266-272. doi: 10.1016/j.fsi.2017.10.056
    [17]
    TAN H Y, CHEN S, HU S. Improvements in the growth performance, immunity, disease resistance, and gut microbiota by the probiotic Rummeliibacillus stabekisii in Nile tilapia (Oreochromis niloticus)[J]. Fish Shellfish Immunol, 2019, 92: 265-275. doi: 10.1016/j.fsi.2019.06.027
    [18]
    BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976, 72: 248-254. doi: 10.1016/0003-2697(76)90527-3
    [19]
    JIN Y, TIAN L, ZENG S, et al. Dietary lipid requirement on non-specific immune responses in juvenile grass carp (Ctenopharyngodon idella)[J]. Fish Shellfish Immunol, 2013, 34(5): 1202-1208. doi: 10.1016/j.fsi.2013.01.008
    [20]
    FOYSAO M J, ALAM M, KAWSER A Q M R, et al. Meta-omics technologies reveals beneficiary effects of Lactobacillus plantarum as dietary supplements on gut microbiota, immune response and disease resistance of Nile tilapia (Oreochromis niloticus)[J]. Aquaculture, 2020, 520: 734-749.
    [21]
    SEWAKA M, TRULLAS C, CHOTIKO A, et al. Efficacy of synbiotic Jerusalem artichoke and Lactobacillus rhamnosus GG-supplemented diets on growth performance, serum biochemical parameters, intestinal morphology, immune parameters and protection against Aeromonas veronii in juvenile red tilapia (Oreochromis spp.)[J]. Fish Shellfish Immunol, 2019, 86: 260-268. doi: 10.1016/j.fsi.2018.11.026
    [22]
    IWASHITA M K P, NAKANDAKARE I B, TERHUNE J S, et al. Dietary supplementation with Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus oryzae enhance immunity and disease resistance against Aeromonas hydrophila and Streptococcus iniae infection in juvenile tilapia Oreochromis niloticus[J]. Fish Shellfish Immunol, 2015, 43(1): 60-66. doi: 10.1016/j.fsi.2014.12.008
    [23]
    CHEN H, LI J, YAN L, et al. Subchronic effects of dietary selenium yeast and selenite on growth performance and the immune and antioxidant systems in Nile tilapia Oreochromis niloticus[J]. Fish Shellfish Immunol, 2020, 97: 283-293. doi: 10.1016/j.fsi.2019.12.053
    [24]
    BROWN M R, BARRETT S M, VOLKMAN J K, et al. Biochemical composition of new yeasts and bacteria evaluated as food for bivalve aquaculture[J]. Aquaculture, 1996, 143(3/4): 341-360. doi: 10.1016/0044-8486(96)01286-0
    [25]
    TUKMECHI A, RAHMATI A H R, MANAFFAR R, et al. Dietary administration of beta-mercapto-ethanol treated Saccharomyces cerevisiae enhanced the growth, innate immune response and disease resistance of the rainbow trout, Oncorhynchus mykiss[J]. Fish Shellfish Immunol, 2011, 30(3): 923-928. doi: 10.1016/j.fsi.2011.01.016
    [26]
    LARA F M, OLVERA N M A, GUZMAN M B E, et al. Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus)[J]. Aquaculture, 2003, 216: 193-201. doi: 10.1016/S0044-8486(02)00277-6
    [27]
    ABU E N M, YOUNIS N A, ABUBAKR H O, et al. Efficacy of dietary yeast cell wall supplementation on the nutrition and immune response of Nile tilapia[J]. Egypt J Aquatic Res, 2018, 44(4): 333-341. doi: 10.1016/j.ejar.2018.11.001
    [28]
    TOVAR R D, ZAMBONINO I J, CAHU C, et al. Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development[J]. Aquaculture, 2004, 234(1/2/3/4): 415-427.
    [29]
    TOVAR D, ZAMBONINO J, CAHU C, et al. Effect of live yeast incorporation in compound diet on digestive enzyme activity in sea bass (Dicentrarchus labrax) larvae[J]. Aquaculture, 2002, 204(1/2): 113-123.
    [30]
    BRATTGJERD S, EVENSEN O, LAUVE A. Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity[J]. Immunology, 1994, 83(2): 288-294.
    [31]
    YUAN X, LIU W, LIANG C, et al. Effects of partial replacement of fish meal by yeast hydrolysate on complement system and stress resistance in juvenile Jian carp (Cyprinus carpio var. Jian)[J]. Fish Shellfish Immunol, 2017, 67: 312-321. doi: 10.1016/j.fsi.2017.06.028
    [32]
    BUTT R L, VOLKOFF H. Gut microbiota and energy homeostasis in fish[J]. Front Endocrinol, 2019, 10(9): 1-5.
    [33]
    XIA Y, CAO J, WANG M, et al. Effects of Lactococcus lactis subsp. lactis JCM5805 on colonization dynamics of gut microbiota and regulation of immunity in early ontogenetic stages of tilapia[J]. Fish Shellfish Immunol, 2019, 86: 53-63. doi: 10.1016/j.fsi.2018.11.022
    [34]
    FERGUSON R M W, MERRIFIELD D L, HARPER G M, et al. The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus)[J]. J Appl Microbiol, 2010, 109(3): 851-862. doi: 10.1111/j.1365-2672.2010.04713.x
    [35]
    KUEBUTORNYE F K A, WANG Z, LU Y, et al. Effects of three host-associated Bacillus species on mucosal immunity and gut health of Nile tilapia, Oreochromis niloticus and its resistance against Aeromonas hydrophila infection[J]. Fish Shellfish Immunol, 2020, 97: 83-95. doi: 10.1016/j.fsi.2019.12.046
    [36]
    LIU W, WANG W, RAN C, et al. Effects of dietary scFOS and lactobacilli on survival, growth, and disease resistance of hybrid tilapia[J]. Aquaculture, 2017, 470: 50-55. doi: 10.1016/j.aquaculture.2016.12.013
    [37]
    ZHANG T, LI Q Q, CHENG L, et al. Akkermansia muciniphila is a promising probiotic[J]. Microb Biotechnol, 2019, 12(6): 11-13.
    • Related Articles

  • Related Articles

    [1]LI Yu, HUANG Jiansheng, CHEN Youming, WEN Zhenwei, OU Guanghai, HUANG Jianpeng, JIANG Xintao, XIE Ruitao, MA Qian, CHEN Gang. Effect of low temperature stress on antioxidant stress, apoptosis and histological structure of gills in cobia (Rachycentron canadum)[J]. South China Fisheries Science, 2023, 19(3): 68-77. DOI: 10.12131/20220227
    [2]CHEN Jie, HE Yang, DAI Xuping, WANG Jun, QING Chuanjie, LI Rui. Histological observation and innate immune barrier study of head kidney of Pelteobagrus vachelli[J]. South China Fisheries Science, 2021, 17(1): 82-90. DOI: 10.12131/20200119
    [3]WANG Jian, ZENG Benhe, XU Zhaoli, ZHANG Bianbian, LIU Haiping, WANG Wanliang, WANG Jinlin, ZHOU Jianshe, HUANG Liping. Effect of dietary protein level on digestive enzyme activity and histological structure in intestine and liver of juvenile Schizopygopsis younghusbandi[J]. South China Fisheries Science, 2019, 15(6): 112-119. DOI: 10.12131/20190107
    [4]MA Dingchang, YE Liuhe, XU Aiyu, PAN Gan, LONG Cheng. A histological study of Tylorrhynchus heterochaetus[J]. South China Fisheries Science, 2014, 10(4): 58-63. DOI: 10.3969/j.issn.2095-0780.2014.04.010
    [5]LIU Zaijun, CEN Jianwei, LI Laihao, YANG Xianqing, HAO Shuxian, WEI Ya, ZHOU Wanjun. Thoughts and prospect of comprehensive utilization of tilapia blood[J]. South China Fisheries Science, 2012, 8(2): 76-80. DOI: 10.3969/j.issn.2095-0780.2012.02.012
    [6]WANG Jingxiang, LI Jia′er, OU Youjun, WANG Yongcui. Ultrastructure of peripheral blood cells of highfin grouper Cromileptes altivelis[J]. South China Fisheries Science, 2011, 7(5): 50-54. DOI: 10.3969/j.issn.2095-0780.2011.05.008
    [7]HU Lingling, LI Jia'er, OU Youjun, YU Na, WANG Gang. Study on microstructure of head-kidney and spleen of Oplegnathus fasciatus[J]. South China Fisheries Science, 2010, 6(3): 41-45. DOI: 10.3969/j.issn.1673-2227.2010.03.008
    [8]LAO Haihua, YE Xing, ZOU Weimin, BAI Junjie, TAN Aiping. Detection of infectious spleen and kidney necrosis virus (ISKNV) of mandarin fish (Siniperca chuatsi) by nested PCR[J]. South China Fisheries Science, 2009, 5(4): 69-72. DOI: 10.3969/j.issn.1673-2227.2009.04.013
    [9]SU Youlu, XU Liwen, FENG Juan, GUO Zhixun, WANG Jiangyong. Study on the morphology of peripheral blood cells of juvenile cobia Rachycentron canadum[J]. South China Fisheries Science, 2007, 3(1): 48-53.
    [10]HUANG Jian-hua, MA Zhi-ming, ZHOU Fa-lin, YE Le, JIANG Shi-gui. Study on anatomy structure and histology of the ovary of wild Penaeus monodon(Fabricius) from the north of South China Sea[J]. South China Fisheries Science, 2005, 1(3): 49-53.

  • Cited by

    Periodical cited type(2)

    1. 安余梁，祁峰，母锐敏，马桂霞. 微藻除磷机理及其工艺模式的研究进展. 工业水处理. 2025(02): 10-17 .
    2. 马林，李春艳，李明泽，罗鑫，丁子元，孟睦涵，徐林通，姜巨峰. 天津市碳汇渔业潜力研究及未来展望. 海洋湖沼通报(中英文). 2024(04): 81-88 .

    Other cited types(2)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(4)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return